Evaluation Of Moisture Content And Salts Concentration For Trametes versicolor Cellulase Production By

Solid State Fermentation Using Sugarcane Bagasse As Substrate

1
Granados-Baeza ,

1
Anzurez-Martnez ,

1
Garca-Maldonado ,

1
Moreno-Alvarez ,

1
Castillo-Cabrera ,

1
Surez-Arzate ,

M-J.
H-L.
J.
F-G.
J-V.
J-C.
E.
Guilbert-Garca1, L-W. Granados-Baeza2, A-B. Prez-Zapata2, A. Alvarez-Castillo1, M-A. Chagolla-Gaona1; 1Inst. Tecnolgico de Zacatepec,
Zacatepec, Morelos, Mexico, 2Univ. Aut. de Campeche, Mexico

Methodology
0.16
0.14

Trametes versicolor was

inoculated in liquid media culture
(3% malt extract, 1% yeast extract, 1%
glucose) @26C, 72 h, 150 rpm.

30 ml
0.12

10 g of sugarcane bagasse were

inoculated with 10 ml of biomass
suspension and 30 mL of water.
Moisture content studied were
60%, 70% and 80%. Saline
solution concentration was
analyzed, adding 10, 15, 20, 25,
30 and 35 mL

Enzyme extract was obtained

using 0.05 M pH 4.8 citrate buffer,
30 min, 4C

0.08

Content of
Salts

Mean

Grouping

10
15
20
25
30
35

9
9
9
9
9
9

0.0416764
0.04273963
0.04284758
0.06156885
0.10990565
0.0560634

A
B
C
D
E
F

20 ml
35 ml
10 ml

Analysis for moisture content for cellulase production by Trametes versicolor

15 ml

Content of
Moisture

Mean

Grouping

60%

0.1050109

0.04

70%

0.14181942

0.02

80%

0.20251432

0.06

ANOVA of salts content for the production of cellulases by Trametes versicolor

Amount of saline solution added

Effect of salt solution

additioned content in the
production of cellulases by
Trametes versicolor.

Degress of
Freedom

Source

Sum of Square

Content of
Salts

0.01763017

Error

4.0913x10-3

12

1.3020x10-3

Total

0.021754317

17

4.8280x10-3

Mean Square

P Evaluate

0.00352603

3.8248053

0.00912475

ANOVA of moisture content for the production of cellulases by Trametes versicolor

Source

Sum of
Squarers

Degress of
Freedom

Mean Square

P Evaluated

Moisture

0.02814768

0.01407384

11.8297

0.00098127

Error
Total

5.74x10-3
0.03388

6
8

6.44x10-3
0.0305173

0.25

0.2

80 %

0.15
70 %

0.1

60 %

0.05

Enzyme production was

determined by calculating amount
of glucose released in mg/mL

25 ml

0.1

Objective
The objective of this work was to evaluate the
moisture content and salts concentration for
Trametes versicolor cellulase production by
solid state fermentation, using sugarcane
bagasse as substrate.

mg. of glucose/mL

Due to depletion of petroleum fuel and the

pollution they cause, bioethanol represents
and interesting alternative. The amount of
linocellulosic waste represents an important
source for obtention of fermentable sugars.
Two steps are required in different
conditions: first, lignocellulosic material
must be degraded; the use of cellulases for
this step is interesting because this process
is environmentally friendly and by the lack
of by-products. The second step is the
fermentation to ethanol. The first step can
be carried out by chemical methods or using
enzymes, like cellulases. The study of
cellulases has a high importance because
there is currently a great interest in using
these enzymes for various industrial
processes such as waste treatment, the
conversion of vegetable waste into fertilizer
or feed, or new processes in the textiles or
the manufacture of detergents, as well as in
brewing and wine, pulp and paper, and
biofuels production (Ghose, 1987). A
method for obtaining cellulases is by solid
state fermentation (SSF), which offers a
number of economic advantages over
conventional submerged fermentation
processes due to low humidity levels and a
high productivity (Aguilar et al., 2001).
Fungi are the preferred organisms due to its
ability to grow in such conditions.

Results

mg. of glucose/mL

Introduction

Analysis for salts content for cellulase production by Trametes versicolor

Moisture Content

Effect of moisture content in the

production of cellulases by
Trametes versicolor

Conclusions
The salts concentration and moisture content can
affect both the microbial growth and cellulase
production, as well as impact on cost at large
scale. This study set up parameters to be included
in further studies for cellulase production.
References
Ghose, t. k. (1987). Measurement of cellulase activities. Pure & App!. Chem.
59(2):260-264
Aguilar, C.N., Augur, C., Favela-Torres, E., Viniegra-Gonzlez, G. (2001) Induction and
repression patterns of fungal tannase in solid state and submerged cultures. Process
Biochem. 36(6) 565-570.