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Metodologia:
Library Preparation
1.Prepare one of the two types of libraries
(Figure 1) for SOLiD System sequencingfragment or mate-paired.
Bead Deposition
4.Deposit 3 modified beads onto a glass slide
(Figure 3). During bead loading, deposition
chambers enable you to segment a slide into
one, four, or eight sections.
Sequencing by Ligation
5.Primers hybridize to the
P1 adapter sequence on
the templated beads
(Figure 4).
6.A set of four
fluorescently labeled dibase probes compete for
ligation to the sequencing
primer.
7.Multiple cycles of
ligation, with the number
of cycles determining the
eventual read length.
8.Following a series of
ligation cycles, the
extension product is
removed and the
template is reset with a
primer complementary to
the n-1 position for a
second round of ligation
cycles.
Primer Reset
9.Five rounds of primer reset are completed
for each sequence tag (Figure 5). Through the
primer reset process, virtually every base is
interrogated in two independent ligation
reactions by two different primers.
Referencias:
http://langebio.cinvestav.mx/labsergen/
http://nxseq.bitesizebio.com/articles/sequencing-bysynthesis-explaining-the-illumina-sequencing-technology/
http://www.appliedbiosystems.com/absite/us/en/home/ap
plications-technologies/solid-next-generationsequencing/next-generation-systems/solid-sequencingchemistry.html?sa=t&rct=j&q=&esrc=s&source=web&cd=9
&ved=0CF0QFjAI&url=http%3A%2F%2Fwww3.appliedbiosy
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OLiDSystemSequencing%2FOverviewofSOLiDSequencingCh
emistry%2F&ei=L5BRUo64K4vS9QTj4C4DQ&usg=AFQjCNEp3FL_otaMG4Yp86AVwynEip8PIQ&s
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