Escolar Documentos
Profissional Documentos
Cultura Documentos
METHODS
2nd Semester, Year 1, 2014
COURSE CONTENT
Understanding of the spectrophotometric
methods in the identification and structure
determination of organic compounds.
UV/VS, IR and FTIR, NMR, various
combinations of chromatographic-mass
spectrometry determinations
BOOKS RECOMMENDED
1. Spectrometric Identification of organic
compounds (by Silverstein et al, John Wiley &
Sons, INC.)
2. Spectroscopic Methods in Organic Chemistry (by
Williams and Fleming, McGraw-Hill Company)
3. Spectroscopic Methods in Organic Chemistry (by
Hesse et al, Thieme)
4. Organic Structural Spectroscopy (by Lamberiet
al, Prentice Hall)
Unit Objectives
At the end of this unit, you should be able to:
1. Explain the principles behind different
spectroscopic techniques.
2. Propose the expected spectroscopic
features of organic molecules.
3. Determine the structure of organic
compounds using different spectroscopic
methods.
What is spectroscopy??
From Wikipedia
Spectroscopy: The study of the interaction between
radiation and matter (i.e. molecules/atoms)
Spectrometry: The measurement of these interactions
Every molecule has it own unique set of spectra. While
similar molecules will have some spectra that look
similar or near identical, there will be one spectrum
that will distinguish them.
The trick is to know what pieces of the puzzle the
different methods reveal, as well as what their
limitations are.
Organic Spectroscopy
The study of the interaction of
electromagnetic radiation with organic
molecules.
Purpose: structure determination of
organic molecules.
Introduction
The full range of electromagnetic radiation
wavelengths is referred to as the electromagnetic
spectrum
Spectrum is the display of the energy level of an
e.m.r. as a function of wave number of
electromagnetic radiation energy.
The energy level of an e.m.r. may be expressed in
any of the following terms:
1 Absorbance (e.m.r. absorbed)
2 Transmittance (e.m.r. passed through)
3 Intensity (radiant power of e.m.r).