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Bergers disease
Kidney disease that occurs when an antibody called Immunoglobin A (IgA) lodges
in the kidney.
Result in local inflammation that, over time, may hamper the kidneys ability to
filter waste, excess water and electrolytes from the blood.
Studyblue.com
Image of a RTEC
Methodology
Renal Biopsy
(sample)
SA--Staining
Statistical Analysis
Nuclei stained with
DAPI
Stained with
fluorescein
isothiocyanate- or
Cy3-conjugated
IgG
Protein expression
of p16, p21, Col III
and FN
blocked and
incubated with
anti- -SMA or antiCol III antibody
together with anti
p16 or anti-p21
antibody
Methodology
SA--gal staining
Boiled in sodium citrate solution for 30 minutes then allowed to cool to room
temperature
Methodology
Methodology
To evaluate the association between p16 and p21 in RTECs and -smooth
muscle antibody (-SMA) and Col III in renal interstitium
Tissue sections were blocked and incubated with anti- -SMA (1:100 dilution) or
anti-Col III antibody (1:100 dilution) together with anti p16 (1:100 dilution) or
anti-p21 antibody (1:100 dilution) at 4 overnight
Samples were mounted with glycerol and visualized under a confocal scanning
microscope
Results
A linear correlation analysis revealed that the expression of senescenceassociated markers was associated significantly with blood pressure and
renal dysfunction but not with patient age, BMI, LDL cholesterol level, or 24h urinary protein value.
Percentages of p16 and p21- positive RTECs and SA--gal positivity were
associated closely with glomerular sclerosis, interstitial fibrosis, and vessel
lesion.
Conclusion