Potential uses of Stem Cell

African Journal of Internal Medicine Vol. 1 (1), pp. 010- 016, 2012. International Scholars
Journals
Full Length Research Paper

Autologus MSC bone marrow stem cell and allogenic pancreatic stem cell for repair of beta
pancreatic cell in experimental diabetes mellitus
Purwati Armand1,2, Fedik. A. R2, Sony Wibisono1, Anas. P2, Eric. H2, Helen. S2, Deya. K2
1Department of Internal Medicine, Faculty of Medicine, Airlangga University Dr. Soetomo Hospital,
Surabaya, Indonesia.
2Stem Cell Laboratory, Institute of Tropical Diseases, Airlangga University, Surabaya, Indonesia.
*Corresponding author. E-mail: purwatipanpan@yahoo.com
Received 23 October, 2012; Accepted 19 December, 2012
Abstract
Alternative therapies in diabetes mellitus (DM) management include the use of stem cells. Stem cells
derived from the bone marrow and pancreatic cells from allogenic donors were used in this
experimental animal model to restore glucose control. DM was induced in Wistar rats with 50 mg/kg
alloxan. DM rats were divided into 4 treatment groups, Group 1 was transplanted with autologous bone
marrow derived mesenchymal stem cells (MSC) by intraperitoneally injection. Group 2 was given
allogenic pancreatic cells intraperitoneally. Group 3 was given insulin subcutaneously, and Group 4
served as control (no treated). The dosage was 200,000 cells/rat. Post therapy results in group 1
revealed significant decrease of blood sugar levels, an increase in insulin levels, and increased C
peptide levels. In group 2, there were more pronounced changes, improvements in glucose control
compared to group 1. Those receiving only insulin the levels of blood sugar decreased but less so
compared to those receiving MSC or pancreatic cells (p = 0.002). In a DM Wistar rat model the
intraperitoneal administration of pancreatic cells resulted in better restoration of glucose control than
intraperitoneally of bone marrow derived MSC, which in turn was better than only insulin.
Key words: Diabetes mellitus (DM), stem cell, allogenic, autologous.

Induced Monocytes derived HSCs (CD34+) with LPS accelerated Homing Rat-Bone Marrow Mesenchymal
(CD105) in Injured Pancreas
1,2Fedik A. Rantam, 1,3Purwati, 6Budi Setiawan, 3Sony Wibisono.,1,4Ferdiansyah.,1.5Joni Wahyuhadi., 1.4
Edward Mouly., 1.4 Dwikora N. Utomo., 1.4 Heri Suroto
1 Stem Cell Lab. Institute of Tropical Disease, Airlangga University Indonesia; 2Virology and Immunology Lab., Dep. of
Microbiology, Faculty of Veterinary Medicine, Airlangga University; 3 Tropic and Infection Division of Internal Medicine,
School of Medicine, Airlangga University; 4Regenerative Medicine, Department of Orthopedic, Dr. Soetomo Teaching
Hospital/ School of Medicine Airlangga University Surabaya; 5 Dep. of Neurosurgary, Dr. Soetomo Teaching Hospital/ School
of Medicine Airlangga University Surabaya; 6Animal Surgary, Animal Hospital, Faculty of Vet. Medicine Airlangga University,
Surabaya-Indonesia.
ABSTRACT
Investigating the function of combining induced rat monocytes derived bone marrow-haemopoietic stem cell (rat BM-HSCs)
with LPS and rat bone marrow- mesenchymal stem cell (rat BM-MSCs) was to use for acceleration of homing process
mechanism in injured pancreas. Mononucleate stem cells were isolated from aspirated whole rat BM using ficoll and
cultured in -MEM complete growth medium in 10 cm petridish. Two days, adherents cell after twice washing in petridish
were added -MEM growth medium and then mesenchymal cells were characterized using CD105 marker in third passage.
Then for haemopoietic Stem Cell (HSCs) were isolated from rat bone marrow with magnetic beads CD34+ and differentiated
in vitro then induced monocyte with LPS. Animal experiments were used 28 male wistar rat, and devided into 4 groups. After
transplantation with combined stem cells were analyzed the expression of pair box gen 4 (Pax4), pancreatic and duodenal
homeobox (Pdx1), c-Peptide used immunocytochemistry and immunohistochemistry, and secreted insulin, c-peptide
used indirect ELISA. Results showed that the expression of Pax4, Pdx1, c-peptide found in the surface membrane cell of
pancreatic cell, and secreted c-peptide and insuline showed significant P<0,05 in transplanted group 2,3 and 4, but in group
3 showed dominant of c-Peptide secretion in group transplanted with combined cell BM-MSCs and induced monocytes
HSCs than non combined cell. Conclusions suggested that combining of induced monocytes derived HSCs and rat BM-MSCs
have been accelerated homing MSCs into injured pancreatic tissue.
Key words: Induced monocyte derived HSCs, rat BM-MSCs, homing, injured pancreas