Escolar Documentos
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Therapy
Johns Hopkins Abx Guide (not free any
more)
Palm
o iSilo program
o Epocrates
Grampositives
Anti-staph PCNs
(nafcillin, methicillin,
oxacillins)
Imipenem
Meropene
m
Amoxicilli
n
Ampicillin
Macrolides
Penicillin G/V
Gramnegatives
Anti-pseudomonal PCNs
(pipercillin, ticarcillin,
carbenicillin)
Clindamycin
Linezolid
Fluoroquinolines
Vancomycin
Rifampin
Sulfonamides
Aztreona
m
Aminoglycosides
Tetracyclines
1st
Generation
Cephalosporin
s3rd
Generation
2nd
Generation
Beta-lactam ABX
o Penicillins
o Cephalosporins
o Aztreonam
o Carbapenems
Exception
o Vancomycin
Beta-lactam structure
Mechanism of Action
1.All beta-lactams bind penicillin-binding
proteins (PBPs)
2.All beta-lactams block transpeptidase
cross-linking of cell wall
3.Activate autolytic enzymes, causing
osmotic damage (bactericidal)
Beta-lactams:
1st mechanism of resistance
Beta-lactamase production (i.e. S. aureus)
We can get around this mode of resistance by
making beta-lactamase resistant penicillins (i.e.
nafcillin)
Beta-lactams:
2nd mechanism of resistance
Change the structure of PBPs
(i.e. Methicillin-Resistant S. Aureus)
Once bugs have changed their PBPs, we only
have one drug that will work, vancomycin.
Beta-lactams:
3rd mechanism of resistance
Efflux pump or change in porin structure:
Relevant for gram-negative bacteria
1st Generation
Drugs
o Penicillin G and V
Clinical use
o Narrow spectrum (mainly gram-positives)
Sensitive to beta-lactamases
o Means: on an exam, penicillin G or V is never the
answer for treating Staph
Exam questions:
o DOC for syphillis (benzathine penicillin),
o DOC in strep infections, especially to prevent
rheumatic fever
o DOC for susceptible pneumococci
2nd Generation
Drugs
o Nafcillin, Methicillin, Oxacillin, Cloxacillin,
Diclaxicillin
To overcome the beta-lactamase resistance,
these drugs were developed but they became so
narrow spectrum that they only clinically are
used for Staph.
These drugs created the superbug MRSA
o Beta-lactamase
o
3rd Generation
Drugs
o Aminopenicillins
Ampicillin
Amoxicillin
Clinical use
o Broad spectrum (gram positive and gram negatives, but NOT
beta-lactamase resistant)
Famous for treating:
H. flu and Listeria (ampicillin)
Lyme Disease (amox) DOC in peds and pregnancy
Enterococci
4th Generation
Drugs
o Anti-pseudomonal penicillins
Ticarcillin
Piperacillin
Carbenicillin
Clinical use
Pseudomonas
Synergistic effect when combined with aminoglycosides.
Parenteral penicillins usually combined with beta-lactamase
inhibitors
Pharmacokinetics of Penicillins
Rule: All penicillins are water soluble, except nafcillin.
Water soluble substances:
o Are excreted by the kidneys.
Means adjustments in renal failure and are potentially renal toxic
Toxicity
Rule: Penicillins cause allergies
o Come from fungal organisms
Means already immunogenic
Toxicity
Jarisch-Herxheimer reaction in Rx of syphilis
o Fever, chills, headache, myalgias, and
exacerbation of syphilitic cutaneous lesions
Ampicillin causes a famous maculopapular rash
when given to patients with infectious mono
(EBV).
Cephalosporins
Mechanism of action and resistance:
o same as penicillins
Clinical use
o Gram positives
And a few gram negatives PEcK (Proteus, E. coli, Klebsiella)
Pharmacokinetics
o Do not enter CNS
Clinical use
o Gram negatives: HEN PEcKS (H. flu,
Enterobacter, Neisseria, Proteus, E. coli,
Klebsiella, Serratia)
Pharmacokinetics
o Do not enter CNS, except cefuroxime
Clinical use
o 1st generation + 2nd generation = 3rd generation (gram positive
and negative) +anaerobes
Pharmacokinetics
o Ceftriaxone is lipid soluble
Means good entry into CNS
Means metabolized and excreted into bowel
Can cause sludge in gallbladder
Boards:
o Ceftazidime for pseudomonaz
o Ceftriaxone for gonorrhea and meningitis
Clinical use
o 3rd Generation + more beta-lactamase
resistance
Toxicity
Same as penicillins
Disuliram-like reaction w/ ethanol
o In cephalosporins with a methylthiotetrazole
group, i.e. cefamandole, cefoperazone,
cefotetan
azole portion gives us the disulfiram-like reaction
Metronidazole
Aztreonam
Mechanism:
o Monobactam resistant to beta-lactamases
o Inhibits cell wall synthesis (same as
penicillins)
o Synergistic with aminoglycosides
Clinical use
o Gram negative rods only (pseudomonas)
Toxicity
o No cross-allergenicity w/ penicillins
Imipenem/cilastatin, Meropenem
Mechanism
o Carbapenems resistant to beta-lactamases
o Inhibits cell wall synthesis (same as penicillins)
o Cilastatin inhibits renal dihydropeptidase I which
decreased inactivation of imipenem in kidney.
Clinical use
o Decerebrate Antibiotics
Dont need to think about coverage, can work on almost
anything
Toxicity
o Imipenem famous for CNS toxicity (seizures)
o Meropenem has reduced risk of seizures
Vancomycin
Mechanism
o Inhibits cell wall mucopeptide formation by binding
o D-ala D-ala portion of cell wall precursors (USMLE TQ)
Resistance occurs when changed to D-ala D-lac
Clinical use
o Gram positive multidrug-resistant organisms
MRSA (IV)
C. difficile (PO)
Toxicity
o Nephro and ototoxic
o Red man syndrome with rapid infusion
Can prevent w/ antihistamine pretreatment
Grampositives
Anti-staph PCNs
(nafcillin, methicillin,
oxacillins)
Penicillin G/V
Imipenem
Meropene
m
Amoxicilli
n
Ampicillin
Gramnegatives
Anti-pseudomonal PCNs
(pipercillin, ticarcillin,
carbenicillin)
50s ribosome
Vancomycin
Nucleus
Aztreona
m
30s ribosome
1st
Generation
Cephalosporin
s3rd
Generation
2nd
Generation
Tetracyclines
Drugs
o Doxycycline
o Minocycline
o Demeclocycline
o Tetracycline
Mechanism
o Reversibly bind to the
Tetracyclines
Clinical use
o Very broad spectrum
o Important use for spirochetes and intracellular bugs
Rickettsial Infections
Chlamydia
Toxicity
o Chelators of divalent ions
Means they deposit in bones and teeth
Means contraindicated in pregnancy and in kids who are still growing
Means cant take with antacids or iron.
o GI distress
o Fanconis syndrome
o Photosensitivity
Boards:
o Doxycycline is lipid soluble; means good STDs and prostatitis
o Minocycline is very water soluble and enters all secretions, especially saliva;
means useful for meningococcus prophylaxis
o Demeclocycline inhibits the release of ADH; means can be used for SIADH
Aminoglycosides
Drugs
o Gentamycin, neomycin, amikacin, tobramycin, streptomycin
Mechanism
o Taken up by an oxygen dependent pump and bind to the 30S ribosomal unit
and Induce the binding of the wrong t-RNA-AA complex, resulting in the
synthesis of false proteins. (Bactericidal)
Aminoglycosides
Clinical use
o Gram negative aerobes only!
(pseudomonas)
o Synergistic w/ beta-lactams
o Neomycin for bowel surgery
o Tobramycin for Pseudomonas
Toxicity
o Amino (NH3) + glycoside (OH) makes
extremely polar
Means membrane penetration in a
bacteria is dependent on a special
oxygen pump and only covers gram
negative aerobes
Means renally excreted and renal toxic
Means can be trapped in inner ear and
is ototoxic
o Neuromuscular blockade
Macrolides
Drugs:
o Erythromycin
o Azithromycin
o Clarithromycin
Mechanism
o Inhibit protein synthesis
by blocking
translocation, bind to
50S ribosomal subunit
(resistance is through
methylation at binding
site)
Macrolides
Clinical use
o Same broad coverage as tetracyclines
o URIs and atypical pneumonias (Mycoplasma,
Legionella, Chlamydia)
o Neisseria
o Alternative for penicillin allergic patients
Toxicities
o Stimulate motilin receptor (erythromycin) causing GI
upset
o Lipid soluble, except azithromycin
Means P450 interactions (erythromycin is a famous inhibitor)
and liver problems (acute cholestatic hepatitis)
Clindamycin
Mechanism
o Blocks peptide bond formation at 50S
ribosomal subunit (bacteriostatic)
Clinical use
o Gram-positives and anaerobes
Means can easily cause C. diff colitis
Linezolid
Mechanism
o Linezolid binds on the 23S portion of the 50S subunit close to the
peptidyl transferase and chloramphenicol binding sites.
Clinical
o Famous for treating gram-positive drug resistant bugs (MRSA,
and multidrug resistant pneumococcus)
Toxicity
o Usually well tolerated
o Thrombocytopenia
o MAOI (avoid tyramine containing food)
Quinupristin/Dalfopristin
Mechanism
o Protein synthesis inhibitors that bind the 50S
ribosomal subunit
Clinical use
o VRE
Toxicity
o P-450 inhibitor
Fluoroquinolones
Rifampin
Sulfonamides
Fluoroquinolones
Drugs
o Ciprofloxacin
o Gatifloxacin
o Levofloxacin
o Moxifloxacin
o Ofloxacin
Mechanism
o Inhibits DNA gyrase (topoisomerase II) (Bactericidal)
Fluoroquinolones
Clinical use
o Gram-negative rods of UTI and diarrhea
o Were 1st oral treatment of gram-negative sepsis
Means were overused, leading to resistance
Toxicity
o QT prolongation and arrhythmias
o Hypo/hyperglycemia
o Achilles tendon rupture or tendinitis has occurred rarely
Rifampin
Mechanism
o Inhibits DNA-dependent RNA polymerase
Clinical use
o TB (in combo and in prophylaxis)
o Famous for prophylaxis of meningococcus and H. flu
Toxicity
o Hepatotoxic
o Revs up P-450
o Rs:
RNA polymerase inhibitor
Revs up P-450
Red/orange body fluids
Sulfonamides
Mechanism
o Inhibits bacterial dihydropteroate synthase
by competing for binding sites with paminobenzoic acid (PABA), a precursor
required for bacterial synthesis of folic acid.
o Trimethoprim binds tightly to bacterial
dihydrofolate reductase. Synergistic with
sulfonamides.
Sulfonamides
Clinical use
o Resistance to sulfonamides is common
o PCP prophylaxis (PO) and treatment (IV)
TrimethoprimSulfamethoxazole, (TMP-SMX)
If sulfa allergy use pentamidine (antiprotozoal agent)
Toxicity
o Allergies (sulfa allergies, hemolytic anemia, SJS)
o Carried by albumin
Means can cause kernicterus
o Crystalluria
o Folic acid can be given to avoid some toxicities
Metronidazole
Mechanism
o Toxic metabolites
Means causes GI disturbance, glossitis (metallic taste in
mouth), urethritis
Clinical use
o Anaerobes
o G.E.T. on the Metro (Giardia, Entamoeba,
Trichomonas)
o C. diff colitis (PO)
Toxicity
o Metronidazole
Disulfiram-like reaction w/ ethanol
Mechanisms of Resistance
Gram-positives
Oddballs
o Staph/Strep
o Listeria
o Bacillus
o Clostridium
o Chlamydia (obligate
o Corynebacterium
intracellular)
o Rickettsia (obligate
intracellular)
o Mycobacterium (acid-fast)
o Treponema (spirochete)
o Borrelia (spirochete)
Grampositives
Gramnegatives
Cell Wall
50s ribosome
Nucleus
30s ribosome
Grampositives
Gramnegatives
Cell Wall
50s ribosome
Vancomycin
Nucleus
30s ribosome
Aztreona
m
Grampositives
Anti-staph PCNs
(nafcillin, methicillin,
oxacillins)
Cell Wall
Gramnegatives
Anti-pseudomonal PCNs
(pipercillin, ticarcillin,
carbenicillin)
Penicillin G/V
50s ribosome
Vancomycin
Nucleus
30s ribosome
Aztreona
m
Grampositives
Anti-staph PCNs
(nafcillin, methicillin,
oxacillins)
Cell Wall
Gramnegatives
Anti-pseudomonal PCNs
(pipercillin, ticarcillin,
carbenicillin)
Penicillin G/V
50s ribosome
Vancomycin
Nucleus
Aztreona
m
30s ribosome
1st
Generation
Cephalosporin
s3rd
Generation
2nd
Generation
Grampositives
Anti-staph PCNs
(nafcillin, methicillin,
oxacillins)
Penicillin G/V
Amoxicilli
n
Ampicillin
Gramnegatives
Anti-pseudomonal PCNs
(pipercillin, ticarcillin,
carbenicillin)
50s ribosome
Vancomycin
Nucleus
Aztreona
m
30s ribosome
1st
Generation
Cephalosporin
s3rd
Generation
2nd
Generation
Grampositives
Anti-staph PCNs
(nafcillin, methicillin,
oxacillins)
Penicillin G/V
Imipenem
Meropene
m
Amoxicilli
n
Ampicillin
Gramnegatives
Anti-pseudomonal PCNs
(pipercillin, ticarcillin,
carbenicillin)
50s ribosome
Vancomycin
Nucleus
Aztreona
m
30s ribosome
1st
Generation
Cephalosporin
s3rd
Generation
2nd
Generation
Grampositives
Anti-staph PCNs
(nafcillin, methicillin,
oxacillins)
Imipenem
Meropene
m
Amoxicilli
n
Ampicillin
Macrolides
Penicillin G/V
Gramnegatives
Anti-pseudomonal PCNs
(pipercillin, ticarcillin,
carbenicillin)
Clindamycin
Linezolid
Vancomycin
Nucleus
Aztreona
m
Aminoglycosides
Tetracyclines
1st
Generation
Cephalosporin
s3rd
Generation
2nd
Generation
Grampositives
Anti-staph PCNs
(nafcillin, methicillin,
oxacillins)
Imipenem
Meropene
m
Amoxicilli
n
Ampicillin
Macrolides
Penicillin G/V
Gramnegatives
Anti-pseudomonal PCNs
(pipercillin, ticarcillin,
carbenicillin)
Clindamycin
Linezolid
Fluoroquinolines
Vancomycin
Rifampin
Sulfonamides
Aztreona
m
Aminoglycosides
Tetracyclines
1st
Generation
Cephalosporin
s3rd
Generation
2nd
Generation
fluoroquinolones, sulfonamides.
Toxicity in Pregnancy
Recommendation
Cautiona
Chloramphenicol
Caution at term
Fluoroquinolones
Caution
Clarithromycin
Teratogenicity in animals
Contraindicated
Ertapenem
Caution
Erythromycin
estolate
Imipenem/cilastatin
Cholestatic hepatitis
Contraindicated
Caution
Linezolid
Caution
Meropenem
Unknown
Caution
Metronidazole
Caution
Nitrofurantoin
Caution; contraindicated at
term
Quinupristin/dalfop
ristin
Sulfonamides
Unknown
Caution
Caution; contraindicated at
term
Tetracyclines
Contraindicated
Vancomycin
Unknown
Caution
Newbor
n
Adul
t
Practice Question
A 16-year-old high school cheerleader presents
with low grade fever, pleuritic pain and a nonproductive cough. A sample tube of her blood was
placed in ice, and "grains of sand" appeared in the
glass portion of the tube. Therapy should include
which of the following?
A. Ampicillin
B. Erythromycin
C. Oxygen and external cooling
D. Penicillin G
E. Ribavirin
Practice Question
A 58-year-old alcoholic man with multiple dental caries
develops a pulmonary abscess and is treated with
antibiotics. Several days later, he develops nausea,
vomiting, abdominal pain, and voluminous green diarrhea.
Which of the following antibiotics is most likely responsible
for this patient's symptoms?
A. Chloramphenicol
B. Clindamycin
C. Gentamicin
D. Metronidazole
E. Vancomycin
Practice Question
Which of the following organisms is most
likely to be implicated as a cause of
urethritis that persists after antibiotic therapy
for gonorrhea?
A. Actinomyces
B. Chlamydia
C. Mycobacteria
D. Nocardia
E. Rickettsia
Practice Question
A 33-year-old woman presents with fever, vomiting, severe
irritative voiding symptoms, and pronounced costovertebral
angle tenderness. Laboratory evaluation reveals
leukocytosis with a left shift; blood cultures indicate
bacteremia. Urinalysis shows pyuria, mild hematuria, and
gram-negative bacteria. Which of the following drugs would
best treat this patient's infection?
A. Ampicillin and gentamicin
B. Erythromycin
C. Gentamicin and vancomycin
D. Tetracycline
Practice Question