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Entamoeba histolytica
Entamoeba histolytica
Morphology & Laboratory
Identification - trophozoites range 12 to
30 microns in diameter; nucleus has an
even distribution of peripheral chromatin
and a small, compact, centrally located
karyosome; cytoplasm is smooth and
granular; inclusions, if present, are red
blood cells; cysts range 10 to 20 microns in
diameter and contains four nuclei when
mature. Cigar-shaped chromatoid bars may
be present in some cysts.
Entamoeba hartmanni
Epidemiology
- similar to E.
histolytica
Formerly called the small race of
Entamoeba histolytica.
Technologists must be able to
differentiate this organism from E.
histolytica because E. hartmanni is
non-pathogenic.
Morphology & Laboratory
Identification - This organism is
morphologically similar to E.
histolytica. The difference lies in the
sizes of the respective organisms.
Trophozoites will measure less than 12
microns, while cysts will measure less
than 10 microns.
Entamoeba coli
Entamoeba gingivalis
Infective site - the mouth; the
organism thrives in diseased gums,
but is not considered a causal agent.
It is destroyed in stomach if
swallowed.
Transmission - contact with
fomites (drinking glasses, eating
utensils, etc.); kissing.
Morphology - resembles E.
histolytica, but has no cyst stage. It
is the only species which ingests
leucocytes.
Endolimax nana
Occurrence
Iodamoeba btschlii
Pathogenicity
- none.
Morphology - the cyst is often
called the iodine cyst due to the
presence of a large glycogen vacuole
which stains dark brown with iodine.
Trophozite is usually 9 to 14 m
long, ranging from 6-20 m.
Entamoeba polecki
- is a parasite of pigs and monkeys.
Rarely, it may infect humans. It can be
distinguished from E. histolytica in that
its cyst is consistently uninucleated. In
stained fecal smears, the nuclear
membrane andd kayosome are very
prominent.
Trophozoite
Size: 10 to 25m; usual range, 12-18m.
Motility: similar to that of Entamoeba
coli.
DIAGNOSIS
Diagnosis is made through stool examination.
Liquid stools will show trophozoites, while formed
stools will show cysts. Direct fecal smears may be
done to demonstrate trophozoites. Formalin-ether
concentration technique and iodine stain are useful
o differentiate species.
TREATMENT
No treatment is necessary because these
amebae do not cause disease.
EPIDEMIOLOGY
In single stool examinations of over 30,000
Filipinos, the prevalence of Entamoeba coli was
about 21% Endolimax nana about 9% and
Iodamoeba butschlii, 1%.