Escolar Documentos
Profissional Documentos
Cultura Documentos
Vanessa Aguilar
Project Plan
October 27, 2010
Meningitis
Cerebral spinal fluid
leak
Pseudomeningocele
Arachnoiditis
Epidural abscess
http://www.meningitistrust.ie/Meningitis.html
Current dural
replacement
market
History of dural
replacement
http://www.nlm.nih.gov/medlineplu
s/ency/imagepages/17146.htm
Gore-Tex (ePTFE)
Neuropatch (polyester
urethane)
Duragen (Collagen)
DuraSeal (PEG-based
spray)
Nasser, R. et al. Covidien
Tisseel (Fibrin/trombin
http://www.emmgraphics.com/projects/covidien/spineseal
solutions)
/pdfs/09_0924jallocase.pdf
http://www.medcompare.com/details/16911/DuragenDural-Graft-Matrix.html
Preclude (PTFE/
elastomeric
1. Stendel et al. J Neurosurg, 2008. 2. Cammisa et al, Spine.
fluoiropolymer)
2000
http://members.cox.net/injections/images/esi_images/roo
ts.jpg
DuraGen
(Integra Life Sciences)
Synthecel Dura
(Synthes)
DuraSeal
(Covidien)
Adherus
(HyperBranch)
Description
DuraGen/DuraGen
Plus is an innovative
matrix designed to
prevent peridural
fibrosis and adhesions
Cellulose
microbially grown
cellulose
PEG hydrogel
Synthetic surgical
sealant PEG
hydrogel blend
Properties
Collagen based
Added cellulose layer
for suturable
performance
100% synthetic
Water-tight sealant
to be applied during
cranial or spinal
surgeries for dura
repair
CE approved for
spinal applications
Advantages
Spray-use, easy to
use
Disadvantage
s
Very expensive
Timely to grow
Cannot be grown
mass-scale
Set up required
Synthetic
Can be
procoagulant
Nerve compression
may ocur1
Set up required
Synthetic
Can be
procoagulant
Model
Plan of Work
GOAL: To develop composite, dual-functioning materials that would
serve to encourage healthy cell growth, wound healing and inhibits
post-surgical scar tissue formation for neural applications. We aim to
develop an all-in-one product to replace dural tissue as well as
support healthy healing.
AIM 1: Develop and
AIM 2: Develop bilayer
characterize suturable biofunctionalized HAanti-adhesion film /
based film
foam
Biocompatible
Biocompatible
Bioabsorbable
Non-immunogenic
Non-immunogenic
Non cell-adhesive /
Dual functioning
Regenerative
cytotoxic
Inhibits protein
Anti-adhesive
Mechanically robust
absorption
Mechanically robust Cost effective
Watertight / sealing Clinically sized
Anti-fibrotic
Repositionable
Plan of Work
GOAL: To develop composite, dual-functioning materials that would
serve to encourage healthy cell growth, wound healing and inhibits
post-surgical scarred tissue formation for neural applications. We
aim to develop an all-in-one product to replace dural tissue as well as
support healty healling.
AIM 1: Develop and
AIM 2: Develop bilayer
characterize suturable biofunctionalized HAanti-adhesion film /
based film
foam
Biocompatible
Biocompatible
Bioabsorbable
Non-immunogenic
Non-immunogenic
Non cell-adhesive /
Dual functioning
Regenerative
cytotoxic
Inhibits protein
Anti-adhesive
Mechanically robust
absorption
Mechanically robust Cost effective
Watertight / sealing Clinically sized
Anti-fibrotic
Repositionable
Material Properties
Hyaluronic Acid
Alginate
http://www.madsci.org/posts/archives/apr2001/986571103.Bc.1.gif
Biocompatible
Bioabsorbable / non-immunogenic
(non-animal)
Very non-cell adhesive, polyanionic,
hydrophilic
Antifibrotic1 (1% HMW HA)
Pro-angiogenic
Shown to reduce adhesion formation
in animals and humans2
Clinically used to reduce adhesions:
Seprafilm, most effective and widely
used anti-adhesion barrier on the
market
Biocompatible
Low toxicity
Gels at physiological pH and
temperature
Very non-cell adhesive, polyanionic,
hydrophilic
Poorly immunogenic (depends on
alginate purification)3
1. Massie et al, The Spine Journal,2005.. 2. Zawaneh et al, Tissue Eng Part B 2008. 3 Dusseault et al. Wiley InterScience, 2005
www.biomedcentral.com
Results
Alginate
Alginate /GMHA
50
25 Adhesion
% Cell
0
Control
Cytotoxicity
1. Culture fibroblast
cells
3. Place Alginate / HA
film on cell medium
4. Wait 24
hours
5. Place Alginate / HA
film supernatant on top
of cells
4. Wait 24
hours
4. Stain coverslips with
calcein / ethidium to label live /
dead cells.
5. Evaluate
cytotoxicity
www.biomedcentral.com
Results
Cytotoxicity
100
90
80
70
60
50
% Cells
40
30
20
10
0
Control
Alginate
Alg-GMHA
Live
Alginate
Alginate /GMHA
Dead
Control
Antifibrotic studies
(using laminectomy model)
1. Collect the tissue
2. Dehydrate in ethanol
3. Acid decalcify
4. Wait for 3 days
5. Slice every 50 um
http://freepages.genealogy.rootsweb.ancestry.com/~gomery/gomorigeo.html
Watertight Studies
Manometer
Acknowledgments
Current Technologies
Autologous grafts
Pericranium or temporal fascia
Xenografts and allografts
Menengitis and Creutzfeldt-Jacobs Disease
Natural and syntethic materials
Gore-Tex (ePTFE)
Neuropatch (polyester urethane)
Duragen (Collagen)
DuraSeal (PEG-based spray)
Tisseel (Fibrin/trombin solutions)
Preclude ( PTFE/ elastomeric fluoiropolymer)
http://www.medcompare.com/details/16911/DuragenDural-Graft-Matrix.html
http://medgadget.com/archives/2005/04/duraseal.html
Results
Cytotoxicity
100
90
80
70
60
50
% Cells
40
30
20
10
0
Control
Alginate
Alg-GMHA
Live
Alginate
Alginate /GMHA
Dead
Alginate /GMHA/HA
Control