Practicum of Autonomic

Drugs
Ria Tustina Hendrayani (G0012179)

Why we use albino rabbits in this experiment?

The rabbit is most commonly used in eye irritancy tests, because it is
cheap,
easy to handle and has a large eye for assessing results.
It has fewer tears than other animals. It also has fewer eye pigment
so that the
effect
is easily
observed.differences which make the rabbit
But
there
are serious

eye a bad model for the human eye. These include the
following:

1. Unlike man, the rabbit has a nictitating membrane, or third eyelid. It

could be argued that this removes irritants from the eye, or, on the
other hand, serves as a trap for material beneath it.
2. The rabbit produces tears less effectively than people, so differences
in the degree and duration of contact of an irritant can be expected.
This may affect test results.
3. The acidity (pH) and buffering capacity of the aqueous humour in
the eyes of human beings and rabbits are different In humans, the pH
is 7,1 7,3 whilst in rabbits it is 8,2. It is thought this difference might
explain the particular susceptibility of the rabbit iris to chemical
inflammation.
4. The thickness, tissue structure and biochemistry of the rabbit and
human cornea are different. The thickness of the cornea in man is 0,51

In the pupil reflex, after the nerve fibers continue in

colliculus superior, nerves will end in pretektal area.
Intercalation neurons associated with the nucleus
Eidinger-Westphal (parasympathetic) on both sides led to
consensual light reflex. Efferent motor nerve of EidingerWestphal nucleus and oculomotor nerve (N.III) enter into

Pilocarpin (parasympathomimeticalkaloid plants)

Source
pharmacodynamic
pharmacokinetic
Indication, contraindication
pasologi

Atropin (parasympatholitik- muscarinic

antagonist)
Source
pharmacodynamic
pharmacokinetic

Ways of Working
calmin
g
shaving the rabbit
eyelashes

Measure and
observe
1. horizontal pupil
2. vertikal pupil
3. Blood vasa
4. corneal reflex
5. consensual reflex

Measuring pupil albino

rabbits (horizontally and
vertically), when
irradiated / not.

3 drops pilocarpin
HCl 2%

3 drops Atropin sulfat

2%

interpretation of results
The effects of
pilocarpine HCl

1. horizontal pupil
Miosis
2. vertikal pupil
3.Blood vasa
Vasodilatation
4.corneal reflex
+
5.consensual reflex

The effects of
atropine sulfate

1. horizontal pupil
mydriasis
2. vertikal pupil
3.Blood vasa vasoconstriction
4.corneal reflex
+
5.consensual reflex
-

the results table

Note: after drug intake, wait 2-3 minutes to get the effect of
drugs

Daftar Pustaka
Gilroy Jhon. Abnormalities of Pupillary
Light Reflex. In : Basic Neurology. Third
edition. New York : Mc Graw-Hill. 2000.
p26 27.
Iaapea. Draize Eye Irritancy Test. The
International Association Against
Painful Experiments on Animals. 2015.
Farmakologi FKUNS. Obat-obat yang
Berpengaruh pada Sistem Syaraf
Otonom. 2014

Thank You