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PEMBENTUKAN
URIN
Rahmatina B. Herman
Bagian Fisiologi
Fakultas Kedokteran Universitas Andalas
Urinesystem
Formation
The urinary
forms the
urine and carries it to the outside
that consists of:
- The kidneys as the urine
forming organs
- The structures that carry urine
from kidneys to the outside for
eliminating from the body
Three basic processes in urine
formation:
1. Filtration by glomerolus
Filtration By Glomerolus
Glomerular capillaries:
impermiabel to protein
Glomerular filtrates:
- protein-free
- concentration of materials that do not bind with
protein as same as in plasma
Filtration rate
of glomerular capillary >> other capillaries, because
of greater in:
- hydrostatic pressure
- glomerular filtration coefficient (Kf)
product of permeability and effective filtration
surface area of glomerular capillary
Afferent arteriole
Efferent arteriole
Capillary
pore
..Filtration By
Glomerolus
The factors governing filtration across
glomerular capillaries (GC) are the same
as all other capillaries
For each nephron:
- Glomerular filtration coefficient (Kf)
- Mean hydrostatic pressure in GC (PGC)
- Mean hydrostatic pressure in Bowmans
capsule (PT)
- Colloid osmotic pressure of plasma in GC ( GC)
- Colloid osmotic pressure of filtrate (T)
protein free
GC
Glomerular capillary
PT
Bowmans capsule
Changes in Kf
..Filtration
Filtration fraction:
- Fraction of plasma flowing through
glomeruli that is filtered into tubules
GFR
- Ratio of GFR to renal plasma flow (RPF)
RPF
- Normal: 0,20
it means: 20 % of plasma that enters glomeruli
is filtered by glomerular capillaries
Filterability
Filterability of solutes is
determined by:
- Size/ molecular weight (MW)
- Electrical charge:
Negative charge is more difficult
than positive charge, because
basement membran of
glomerular capillary consists
proteoglican with negative charge
..
Filterability
Filterability of substances by GC decreases
with increases MW
Substance
MW
Filterability
Water
Sodium
Glucose
Inulin
Myoglobin
Albumin
18
23
180
5.500
17.000
69.000
1,0
1,0
1,0
1,0
0,75
0,005
Passive transport:
- through intercellular space
- using carrier
Osmosis: water
Transport in Proximal
Tubules
Proximal tubule epithelial cells are highly
metabolic and have large numbers of mitochondria
to support potent active transport processes
Proximal tubule epithelial cells have extensive
brush border on the luminal side and also
extensive labyrinth of intercellular and basal
channels extensive surface area for rapid
transport
Epithelial brush border is loaded with protein
carrier molecules and a large number of sodium
ions secondary active transport (co-/ counter
transport)
So, it is the most active reabsorption process
Water moves across membrane by osmosis
Reabsorption in Proximal
Tubule
In the first half
of proximal tubule:
- sodium is reabsorbed by co-transport
along with glucose, amino acids, and
other solutes
- leaving behind solution that has
higher chloride concentration flow to
the second half of proximal tubule
Secretion in Proximal
Tubule
Proximal tubule is important site for
secretion of many substances that must
be rapidly removed from body, such as:
- organic acids and bases
- end product of metabolism
- many potentially harmful drugs or toxin
- para-aminohippuric acid (PAH)
Normal person can clear 90 % of PAH from
plasma flowing through kidneys and
excrete it into urine
So, PAH clearance can be used as index of
renal plasma flow (RPF)
..Transport in Loop of
Henle
The descending thin segment:
- Highly permeable to water
- Moderately permeable to most solutes,
including urea
and sodium
- impermeable to water
- reabsorption capacity is very low
- impermeable to water
- highly metabolic active reabsorption of
Na, Cl, K (25%)
- has Na-H counter transport mechanism
Transport
in
Distal
Tubules
The very first portion of distal tubule
forms part of juxtaglomerular complex
that provides feedback control of GFR
and blood flow in the same nephron
The next early part of distal tubule is
highly convoluted and has many of the
same reabsorptive characteristics of
the thick segment of ascending limb of
loop of Henle:
- avidly reabsorbs most of ions
including Na, Cl, K
- virtually impermeable to water and
urea
The secondin
half
of distal
tubule
and the
Transport
Late
Distal
Tubules
subsequent
cortical
collecting
tubule have
and
Cortical
Collecting
Tubule
Countercurrent Mechanism
Countercurrent mechanism produces
hyperosmotic renal medullary interstitium
concentrated urine
Countercurrent mechanism depends on special
anatomical arrangement of the loops of Henle and
vasa recta (specialized peritubular capillaries of
renal medulla)
Basic requirements for forming a concentrated
urine:
- High level of ADH increases permeability of distal
tubules and collecting ducts to water avidly reabsorb
water
- High osmolarity of renal medullary interstitial fluid
osmotic gradient necessary for water reabsorption to
occur in the
presence of high levels of ADH
..Countercurrent
Mechanism
Major factors that contribute to build up of solute
concentration into renal medulla:
1.
Active transport of Na+ and co-transport of K +,
Cl - and other ions out of thick limb into medullary
interstitium
2.
Active transport of ions from collecting ducts
into medullary interstitium
3. Passive diffusion of large amounts of urea from
inner medullary collecting ducts into medullary
interstitium
4. Diffusion of only small amounts of water from
medullary tubules into medullary interstitium, far
less then reabsorption of solutes into medullary
interstitium
Active
NaCl
Transpo
rt
0
+++++
Distal tubule
Cortical collecting
tubule
Inner medullary
+
+
Permeability
H2O NaCl
+++
++
0
0
+
ADH
+
ADH
+
Ure
a
+
0
+
0
0
++
Thank You