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Biochemistry Departement
Medical Faculty Of Andalas
University
Padang
Oxygen Transport
AttheLungLevel
AttheTissueLevel
Hemoglobin
Each heme molecule is capable of binding
with 1 O2 molecule and each globin
molecule is capable of binding with 1 CO2
molecule.
So, each molecule of Hb can bind to either 4
molecules of O2 and 1 molecule of CO2
100 ml of blood has about 15 gm of Hb, at
Hct = 0.45
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Hb O2 HbO2
HbO2 O2 Hb(O2 ) 2
Hb(O2 ) 2 O2 Hb(O2 ) 3
Hb(O2 ) 3 O2 Hb(O2 ) 4
Equilibrium constants for different reactions
different
Binding of first O2 relatively low affinity
2nd, 3rd and 4th - much higher affinity
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Oxygen as Oxyhemoglobin
Each gram of Hb can store about 1.34 ml
of O2:
1 L of blood (150 gm of Hb) can store
about 208 ml of O2 Oxygen Capacity of
Hb.
With normal cardiac output, about 1040 ml
of O2 can be carried in blood per minute. (4
times of the metabolic demands).
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12
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O2 Saturation.
Units: percent.
Fraction or percentage of all the
hemoglobin binding sites that are
currently occupied by oxygen.
Oxygen Saturation of Hb
O 2 combined with Hb
SHbO2 (% saturation)
100
O 2 capacity of Hb
15
Temperature
[2,3-DPG]
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Exercise
Increase temperature
Increased PCO2 and
Decreased pH (acidosis)
2,3-DPG
2,3-DPG is a glycolytic intermediate
accumulates to uniquely high levels in RBCs
-Increased 2,3-DPG
-Decreased 2,3-DPG
right shift
left shift
Carbon Dioxide
Transport
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AttheTissueLevel
25
AttheLungLevel
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Bicarbonate in RBCs.
Carbonic anhydrase is present in RBCs
CO2 forms carbonic acid which
dissociates to H+ and HCO3CO 2 H 2 O
H 2 CO 3
H HCO 3
Carbonic Anhydrase
PercentofthetotalPaCO2:70%
PercentofthetotalPaCO2:23%
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Pelepasan CO2
Dilakukan oleh:
1. isositrat dehidrogenase
2. -ketoglutarat dehidrogenase
Pelepasan CO2 tidak mengkonsumsi
oksaloasetat.
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Siklus ATP/ADP
Siklus ATP/ADP
ATP
CO2
Pernapasan:
pembentukan energi
dari; - karbohidrat
- lemak
- protein
Penggunaan energi:
- biosintesis makromolekul
- kontraksi otot
- transpor ion aktif
- termogenesis
O2
ADP + Pi
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Fosforilasi Oksidatif
-oksidasi
ATP
O2
glukosa
Asetil KoA
SAS
2H
H 2O
rantai respirasi
ADP
Asam amino
mitokondria
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Resources
BIOEN
589:
Integrative
Physiology.
Download
24 jan 05.
Kennelly, PJ., Rodwell, V W. Proteins: Myoglobin & Hemoglobin. In:
Harpers Illustrated Biochemistry. 27th Ed. 41- 8.
Miliefsky, M. Respiratory System Ch.23. Download 24 Nov 10.
Sheardown, H. Blood Biochemistry. McMaster University. Download
20 Mei 07.
Irvin, CG. Respiratory Physiology. Lecture 4A CO2 Transport. In:
MEDICAL PHYSIOLOGY 30. Download 22 Jun 09.
Marks, DB., Marks, AD., Smith CM. Basic medical biochemistry: a
clinical approach. 1996. Dalam: B.U. Pendit, penerjemah. Biokimia
Kedokteran Dasar: Sebuah Pendekatan Klinis. Eds. J. Suyono., V.
Sadikin., L.I. Mandera. Jakarta: EGC, 2000
R.K. Murray, D.K. Granner, P.A. Mayes, V.W. Rodwell Harpers
Biochemistry. 27th ed. McGraw-Hill Companies, New York. 2006.
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