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Viscoelasticity of Chicken
Femoral Cartilage
Group 7
Priyanka Parajuli, Hemali Patel, Catherine Porter, Shri Rajan, Linh
Phan
INTRODUCTION:
VISCOELASTICITY
Viscosity
Fluid Properties
Gradual Deformation:
Time-dependent
Equation: = ()
Elasticity
Solid Properties
Instantaneous Deformation:
Time-independent
Equation: =(, )
http://wweb.uta.edu/faculty/ricard/Classes/KINE-3301/Notes/Lesson-
INTRODUCTION: ARTICULAR
CARTILAGE
Function:
Composition:
60-85% water by weight
15-22% type II collagen by weight
Various electrolytes (Na+, Cl-, Ca++)
Proteoglycans and chondrocytes
INTRODUCTION: ARTICULAR
CARTILAGE PROPERTIES
Inhomogeneous
Multiphasic Material
Anisotropic
Resistant to Compression
http://www.mdpi.com/2079-4983/3/4/799
ttp://www.spandidos-publications.com/etm/8/5/1357
INTRODUCTION: MAXWELL
MODEL
Spring and dashpot connected in series
Fluid viscoelastic model
Equation:
http://polymodmw.csi.muohio.edu/wp-
http://polymodmw.csi.muohio.edu/wpcontent/uploads/2013/04/Kelvin-Voigt.jpg
INTRODUCTION: STANDARD
SOLID MODEL
Combine Kelvin-Voigt solid model and Maxwell fluid model to derive complex
viscoelastic model
Equation:
http://polymodmw.csi.muohio.edu/wp-
INTRODUCTION:
INDENTATION
TESTS
Characterize mechanical properties of
cartilage outside the body
Stress-Relaxation Test
Indent material and maintain constant strain
Observe stress response of material
Viscoelastic material responds with initial high stress
and then decreases over time
Load-to-Failure Test
Constantly increasing stress
Last point on curve is rupture, failure strength
Viscoelastic materials typically have same ultimate
and failure strength
ps://www.bsbedge.com/astm/astmd6264d6264m-standard
Ozkaya et al., Fundamentals of biomechanics: Equilibrium, motion, and deformation
MOTIVATION:
Osteoarthritis, sports injuries, etc.
Current replacement grafts and surgical reconstructions often result
in complications and instability that worsen current conditions.
Dynamic environment of the human body requires physical
characterization of articular cartilage in addition to biological
characterization.
Mathematical modeling provides unique insight for further
treatment of cartilaginous injuries.
OBJECTIVE:
To assess the mechanical and viscoelastic
properties of chicken femoral cartilage
and model its viscoelastic behavior as a
mathematical equation.
MATERIALS
Step 1:
Preparation of
the Sample
METHODS: PREPARATION OF
THE SAMPLE
Clean
Chicken
Femur
Cartilage
Remove
periosteal
tissue with
sandpaper
Use iris
scissors
Fill Plumber
Putty in
Aluminum
Cylinder
Use putty to
prevent
leaking of
bone cement
Prepare Bone
Cement
Mix 1:1 ratio
of bone
cement and
self-curing
liquid
Desired
thickness:
toothpastelike
Pot the
Femur
Pour cement
in cylinder
until full
Position distal
side of femur
vertically in
bone cement
Allow cement
to solidify
Hold femur
straight while
cement cures
Keep cartilage
moist with
saline and
gauze
Mark Test
Locations
Use marker
to indicate
locations for
stress
relaxation
and load-tofailure tests
Cartilage
Placement
for Testing
Align cartilage
perpendicular
to the
indenter
Indenter is
2.5mm
Calibration
of Load Cell
Calibrate
15kN load cell
to 1.5kN total
load
Indentation
Relaxation
Remove load
Let cartilage
relax to initial
thickness
METHODS: LOAD-TO-FAILURE
TEST
Initial Thickness
Measurement
Use needle and
caliper to measure
thickness of
cartilage at marked
spot
Cartilage
Placement for
Testing
Align cartilage
perpendicular to
the indenter
Indenter is 2.5mm
Calibration of
Load Cell
Calibrate 15kN
load cell to
1.5kN total load
Indentation
Use MTS 858
MiniBionix to
indent until failure
Indent at
2mm/min. while
sampling at a rate
of 100Hz
Model:
axial displacement
Relaxation
Modulus:
tp://www.ncbi.nlm.nih.gov/pubmed/19615781
Wiechert
Parameters:
0.203
Correlation Coefficient
R=
R=0
R=
0.939
R=0
Stress Equation:
RESULTS: LOAD-TO-FAILURE
TEST
Group
Number
Group 6
Ultimate
Stress (Pa)
1.55E+07
Group 7
2.24E+07
Group 8
1.96E+07
Group 9
1.40E+07
RESULTS:
GROUP
ELASTIC
MODULI
Group
Number
Group 6
Elastic
Moduli (Pa)
2.42E+04
Group 7
8.37E+04
Group 8
2.05E+04
Group 9
3.92E+04
DISCUSSION
Difference
between
Stress-Relaxation Graphs
Various chicken cartilage samples
Different thicknesses
Different properties
Wiechert Model
Processing
R= 0.939 for our Wiechert model
Coefficients are dependent on
Trial and error method
Need to alter and per group manually
Not a standardized model-fitting tool
Possible Sources of
Variance:
Chicken age
Cartilage moisture
Chicken gender
Chicken physical activity and health
DISCUSSION (CONT.)
9th Degree Polynomial Best
Fit Line (MATLAB Curve
Fitting Tool)
Correlation Coefficient =
Correlation Coefficient =
DISCUSSION (CONT.)
Statistical Analysis
Mechanica
l
Properties
Failure
Stress
Elastic
Modulus
Average
(Pa)
1.79E+07
4.19E+04
Standard
Deviation
(Pa)
3.85E+06
2.90E+04
Thickness
measurement
Machine error
Bone alignment
Overlap of stressrelaxation and load-tofailure test locations
REFERENCES:
1. Ahearne, M., Siamantouras, E., Yang, Y., & Liu, K.-K. (2009). Mechanical
characterization of biomimetic membranes by micro-shaft poking. Journal of the
Royal Society Interface, 6(34), 471478. http://doi.org/10.1098/rsif.2008.0317
2. Bonifasi-Lista, C., Lakez, S. P., Small, M. S. and Weiss, J. A. (2005), Viscoelastic
properties of the human medial collateral ligament under longitudinal, transverse and
shear loading. J. Orthop. Res., 23: 6776. doi: 10.1016/j.orthres.2004.06.002
3. Kaufman, J. D., Miller, G. J., Morgan, E. F., & Klapperich, C. M. (2008). Time-dependent
mechanical characterization of poly(2-hydroxyethyl methacrylate) hydrogels using
nanoindentation and unconfined compression. Journal of Materials Research, 23(5),
14721481. http://doi.org/10.1557/JMR.2008.0185
4. Machiraju, C., Phan, A. -V., Pearsall, A. W., & Madanagopal, S. (2006). Viscoelastic
studies of human subscapularis tendon: Relaxation test and a Wiechert model.
Computer Methods and Programs in Biomedicine, 83(1), 29-33.
5. Ozkaya, N., Nordin, M., Goldsheyder, D., & Leger, D. (2012). Fundamentals of
biomechanics: Equilibrium, motion, and deformation (3rd ed.). New York: Springer.