DETECTION OF FOOD SPOILAGE

MICROORGANISMS USING

METABOLOMICS
GUIDED BY:

PRESENTED BY:

Mrs. RESHMI RAJAN

LAKSHMI C B

Asst. Professor

S7 FT

Food Technology Dept.

INTRODUCTION
Metabolomics is a novel biochemical tool for
biological studies
It is a non-target approach used to detect,
identify and quantify all the metabolites of an
organism.
Metabolomics has been successfully applied to
different areas of food science.

OBJECTIVES
A brief study about:
Metabolomics
Metabolomics tools
Applications in food analysis

METABOLOMICS
Metabolomics is the study of
metabolites
Metabolites are the end products of cellular
processes and represent the ultimate
reflection of the response of biological
systems to genetic and environmental
change

It is a hypothesis generating tool that

makes use of different analytical

DETECTION INSTRUMENTS
Mass-spectrometry (MS) and nuclear magnetic
resonance (NMR) spectroscopy are the two main
detection methods
MS in unison with other advanced
separation approaches, has found
application in a wide diversity of applied
food science approaches.
Currently, These highly sensitive and reproducible
analytical instruments can analyse over 1000

APPLICATION IN FOOD
MICROBIAL DETECTION
These sophisticated tools are able to detect
metabolites produced during the early stages of
microbial growth
Usually a set metabolites is produced and the organisms
can be identified by analysing these sets
Set of 16 metabolites of Salmonella typhimurium was
detected from pork sample
Set of 16 metabolites of Botrytis allii was identified from
onion in its post harvest stage

OTHER APPLICATIONS
Toxicity assessment/toxicology
food component analysis
quality/authenticity assessment
food consumption monitoring

REFERENCES

Farhana R. Pinu,(2016). Early detection of food pathogens

and food spoilage microorganisms: Application of
metabolomics, Trends in Food Science & Technology(2016)

Djuro Josic1,2*, Dajana Gaso Sokac3, Martina Srajer

Gajdosik3, James Clifton4, (2008). MICROBIAL OMICS FOR
FOOD SAFETY, Journal of Hygienic Engineering and Design
116, Review paper, UDC 579.67

AC Kushalappa,1* A Vikram1 and GSV Raghavan2 (2008).

Metabolomics of headspace gas for diagnosing diseases of
fruits and vegetables after harvest, Stewart Postharvest
Review 2008

Max Rubner Conference 2011, Food Metabolomics, October

9-11, 2011, Karlsruhe, Germany

Al-Kharousi, Z. S., Guizani, N., Al-Sadi, A. M., Al-Bulushi, I.

M., & Shaharoona, B. (2016). Hiding in fresh fruits and
vegetables: opportunistic pathogens may cross