Escolar Documentos
Profissional Documentos
Cultura Documentos
Technology
DNA Extraction
Chemical treatments cause
cells and nuclei to burst
The DNA is inherently
sticky,
sticky and can be pulled
out of the mixture
This is called spooling
DNA
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Spooled DNA
Cutting DNA
Electrophoresis
DNA can be
separated based on
size and charge
The phosphate groups
are negatively
charged
DNA is placed in a
gel and electricity is
run through
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Electrophoresis
Negative DNA moves toward
the positive end
Smaller fragments move
farther and faster
Electrophoresis
DNA
Sequencing
The result is
DNA fragments
of multiple
sizes with
colors that can
be identified
10
DNA Sequencing
After the gel separates
the resulting fragments
by size, we 'read' the
sequence from bottom to
top.
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Copying DNA
Polymerase Chain Reaction
Also called PCR
A method of making many
copies of a piece of DNA
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Copying DNA
The tube is cooled, and
DNA polymerase adds new
bases to the separated
strands
15
PCR
Cloning
CloneClone a member
of a group of
genetically
identical cells
May be produced
by asexual
reproduction
(mitosis)
17
Cloning organisms
A body cell from one
organism and an egg cell
from another are fused
The resulting cell divides
like a normal embryo
18
Cloning Dolly
19
Human Genome
Project
20
HGP Insights
Only 2% of human genome codes for
proteins (exons)
Other 98% (introns) are non-coding
Only about 20,000 to 25,000 genes
(expected 100,000)
Proteome organisms complete set of
proteins
About 8 million single nucleotide
polymorphisms (SNP) places where
humans differ by a single nucleotide
About of genome comes from
transposons (pieces of DNA that move
to different locations on chromosomes)
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Benefits of Human
Genome Project
Improvements in medical
prevention of disease, gene
therapies, diagnosis techniques
Production of useful protein
products for use in medicine,
agriculture, bioremediation and
pharmaceutical industries.
Improved bioinformatics using
computers to help in DNA
sequencing
23
Benefits of Genetic
Engineering
24
Biotechnology
25
Biotechnology
Provides:
Improved food products
Medical advances
An enhanced environment
26
Herbicide Resistant
Crops
+ CP4 EPSPS = Roundup
gene
Ready
Soybeans:
Corn:
Cotton:
Canola:
Roundup Ready
Roundup Ready, Liberty Link
BXN, Roundup Ready
Liberty Link, Roundup Ready
27
Biotechnology Breakthroughs
Insulin (1982)
First commercial biotech product
Reliable, inexpensive source of insulin
Rice
Enriched with beta-carotene and iron
Bananas
Containing edible hepatitis vaccine
28
Biotechnology Breakthroughs
Potatoes with higher solid
content
Garlic that lowers cholesterol
Fruits and vegetables that
reduce risks of cancer and heart
disease
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Environmental Benefits
Reduced pesticide use
Lower energy requirements
Cleaner water
Less soil erosion
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