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Evaluation of cytotoxicity of novel
yamine conjugates on Breast cancer cell
Rohit Shrivastava
MSc Drug Development with Bio-
Breast cancer
• Breast cancer 2nd leading cause of death in
women today.
•
• Most common cancer affecting women.
•
• According to Am. Soc of Cancer, 465,000
women die of cancer annually.
•
• No single factor responsible for majority of
breast cancer. Some risk factors are-
1. Increasing age
2. Family history
3. Previous breast cancer
What are Polyamines?
• Polyamines – chemically active aliphatic amines.
•
• Found in food and also produced by gut
microflora.
•
• Putrescine, Spermidine & Spermine - common
polyamines.
•
• At, physiological pH carry positive charge.
•
• Involved in cell growth, proliferation &
transformation.
•
Effect of etoposide on MCF-7
120
100
% of control (Viable cells)
80
60
40
20
0
0 20 40 60 80 100
Concentration of Etoposide (µM)
MCF-7 were seeded at a seeding density of 5.4 x 105 left for attachment
for 4 hours and 44 hours growth then etoposide was added for an
Exposure time of 48 hours, n=1. All values are mean values.
At 100 µM concentration 73.91 % cells were viable.
Effect of Etoposide on MDA
TAX 30
Cytotoxicity of Etoposide on MDA TAX 30
120
100
% of control (Viable cells)
80
60
40
20
0
0 20 40 60 80 100
Concentration of Etoposide (µM)
MDA TAX 30 were seeded at a seeding density of 5.4 x 105 left for attachment
for 4 hours and 44 hours growth then etoposide was added for an
Exposure time of 48 hours, n=1. All values are mean values.
At,100 µM concentration 74.16 % cells were viable.
Future Plans
• As MCF-7 haven’t reached IC50 . In
next MTT assay planned to increase
concentration up to 200 µM.
•
• Alternative drug for positive control
– Doxorubicin (0-25 µM)
•
• Test the effect of Etoposide and
Doxorubicin in all cell lines.