BACTERIAL GROWTH

JOSEPHINE C. ABRAZALDO

LEARNING OUTCOME
Describe the relationship among
metabolism,catabolism and anabolism
Group the bacteria as to their oxygen requirement
Group bacteria as their temperature requirement
NUTRITIONAL REQUIREMENT
EXPLAIN EXPONENTIAL GROWTH

GROWTH

Is the orderly increase in the sum of all the

components of an organism
Bacteria multiply by binary fission to produce
identical offspring.

Types of growth
Colonies: microscopic product of 20-30 cell
division of a single cell
Turbid suspension: Transformation of a clear
broth medium to a turbid suspension 107 - 10 9
cells per ml
Biofilm: growth is thinly spread over an inert
surface, nutrition is obtained from surrounding
fluid

Doubling time or
Generation time (td )
Time required for any bacterial strain to double
in number under a given set of growth
conditions
Vibrio cholera 13 mins.
V.cholerae can kill a man within 12 hrs where as
MTB takes months to develop

purposes of growing
bacteria in the laboratory

clinical perspective
industrial objective

TCBS: thiosulfate citrate bile

salt sucrose agar
APW: alkaline peptone water

TT Broth:Tetrathionate
broth for Salmonella

ENRICHMENT MEDIA
Promotes the growth of a particular organism by providing it
with the essential nutrients, and rarely contains inhibitory
substances to prevent the growth of normal competitors
SELECTIVE MEDIA

Used for growth of only selected microorganisms. Selection

by
Adding antibiotics, prevents the growth of other cells
Lacking amino acids
May contain stains and color indicators (EMB)

green sulfur
bacteria

purple sulfur
bacteria

The purple sulfur bacteria are a

group of Proteobacteria capable of
photosynthesis, collectively referred
to as purple bacteria. They are
anaerobic or microaerophilic, and
are often found in hot springs or
stagnant water.

C. Hydrogen Ion
concentration

Neutralophiles: grow best at a pH of 6.0 to 8.0

Acidophiles: have optima as low as pH 3.0
Alkaliphiles : have optima as high as pH 10.5

D. Ionic strength and

Osmotic pressure

Halophilic: Organism requiring high salt

concentration
Osmophilic: Organism requiring high osmotic
pressure

BACTERIAL
METABOLISM

obligate aerobes :

catalase, peroxidase,superoxide dismutase

obligate anaerobes:

lack the 3 enzymes

microaerophils : can use low amount of oxygen

they have superoxide dismutase

BACTERIAL VIABILITY

References: Jawetz Medical Microbiology 26th

edition
Murray Medical Microbiology 7th edition
Next topic for advance reading

Microbial death

Sterilization

Antimicrobial agents

Bacterial genetics