Normal Cervix

Taking the Sample

Liquid Based Cytology lab processing

Cytologic screening for cervical cancer

Cervical cancer screening has decreased morbidity and

mortality
Deaths from cervical cancer decreased from 26,000 to

less than 5,000 between 1941 and 1997

Pap smears are not perfect

For a high grade lesion, the sensitivity of a single pap

smear is only 60-80%

Estimated false negative rate is 30-50%
Requires adequate specimen collection
Requires adequate cytological review

Requires adequate patient and physician follow-up

10% of women with cervical cancer had inappropriate

follow-up.
Requires access to care
50% of women with cervical cancer were never

screened and 10% had not been screened within 5 years

of diagnosis.

Who to screen
Any woman with a cervix who has ever had sexual

activity.

When to screen
Start within 3 years of onset of sexual activity or by age

of 21, whichever is first.

Risk factors for cervical dysplasia
Early onset of sexual activity
Multiple sexual partners
Tobacco
Oral contraceptives

Screening frequency
Yearly until three consecutive normal pap smears, then

may decrease frequency to every three years

Annual screening for high-risk women is highly

recommend.

When to stop routine screening

Age 65 and adequate recent screening
Three consecutive normal pap smears
No abnormal pap smears in last 10 years
No history of cervical or uterine cancer

Hysterectomy for benign disease

Hysterectomy for invasive cervical cancer

Original Squamous Epithelium

Vagina and outer ectocervix
4 cell layers
Well-glycogenated (pink) unless atrophic

Columnar Epithelium
Upper and middle endo-cervical canal
Single layer of columnar cells arranged in

folds
Mucin producing (not true glands)

Squamous Metaplasia
Central ectocervix and proximal endocervical canal
Replacement of columnar cells by squamous epithelium
Progressive and stimulated by
Acidic environment with onset of puberty
Estrogen causing eversion of endocervix

Transformation Zone
Zone between original squamo-columnar junction and

the new squamo-columnar junction

Nabothian cysts visually identify the transformation

zone if present

Original Squamo-columnar Junction

Placement determined between 18-20 weeks gestation
Most often found on ectocervix
Can be found in vagina or vaginal fornices
Less apparent over time with maturation of

epithelium

New Squamo-columnar Junction

Border between squamous epithelium and columnar

epithelium
Found on ecto-cervix or in endo-cervical canal
Majority of cervical cancers and precursor lesions

arise in immature squamous metaplasia, i.e. the

leading edge of the squamo-columnar junction

Squamous Epithelium

Parabasal Cells

Intermediate Cells

Superficial

Cells

Endocervix

Endocervical Cells

Endometrial Cells

Non-Epithelial Cells

Lymphocytes

Polymorphs

sperms

Normal smear

Ectropion / Erosion
At puberty & pregnancy the endocervical cells are

pushed out to lie on the ectocervix

Normal

Ectropion

Wide Ectropion

Metaplasia
The endocervical cells are transformed into squamous

cells through the process of squamous metaplasia

Metaplastic Cells

CASE
52 years old woman came tho hospital for
routine
control with
Cc : vaginal discharge
Paps smear : pap grade III