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TRANSLATION
GROUP 5 :
TRECY IVANI
(145090101111016)
NUR RAHMATTULLAH
RETNO P.
(145090101111017)
(145090101111020)
DEPARTMENT OF BIOLOGY
FACULTY OF MATHEMATICS AND NATURAL SCIENCES
UNIVERSITY OF BRAWIJAYA
MALANG
2016
OVERVIEW TRANSLATION
Component of Translation
tRNA
Ribosomal protein
and rRNA
mRN
A
Factor
Component of Translation
proteins:
Amino
Enzyme:
- IF-1, IF-2, IFacid
Peptidyl
3
(initiation
transferase
factors)
- EF-Tu
(elongation
Energy: ATP
factor Tu)
and GTP
- EF-G
(elongation
factor G)
- RF-1 dan RF2
(release
factor)
Structure of tRNA
Alberts et al (2008)
tRNA Charging
Alberts et al (2008)
Structure of rRNA
(Champbell, 2002)
Large subunit
Small subunit
(Champbell, 2002)
(Nierhaus, 2006)
Redundant Effect :
Each triplet codon only code for one AA
Each AA can be coded by several triplet codon
Translation Initiation
Initiation
bind
Shine-Dalgarno Sequence
The sequence interacts with the complementary sequence
in 16S rRNA in the small ribosomal subunit.
Shine-Dalgarno Sequence
1.
2.
3.
4.
fMet-tRNA.
mRNA.
Small ribosome.
Large ribosome.
Elongation Process
1. fMet-tRNA is bound to the AUG
codon at P site.
2. Next codon is positioned in the A
site.
3. Appropriate
amino-acyl
binds to the A site.
tRNA
Elongation Process
6. The bond between the amino acid
and tRNA at P site is broken.
7. A peptide bond is formed between
the free amino acid from the P
site and the one at the A site by:
Peptidyl Transferase
8. When a peptide bond is formed
the free tRNA is in site P and the
tRNA at site A has two amino
acids.
9. Ribosome moves
downstream (3)
one
codon
Elongation Process
Termination Process
Termination is signaled by STOP codon
Termination Process:
1. Stop codons Do Not code for amino
acids
2. Release Factors (RF) which looks like
tRNA binds to the A site
3. Peptide is cleaved by peptidyl
transferase at the P site
Termination Process
Termination Process
POLYRIBOSOME
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