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Culture Media
In the history
Bacterial Media
Selective
Differential
Enriched
Selective Medium
Examples:
MacConkey agar
E. coli on EMB
Differential Medium
Permits the differentiation of organisms
that grow on the medium.
Reveals the presence of 2 or more similar
microorganisms by differences in the
appearance of their colonies.
Examples
Enriched Medium
Broth or solid medium containing rich
supply of special nutrients that promotes
the growth of fastidious organisms.
Prepared by adding extra nutrients to a
medium called nutrient agar.
BAP
CHOC
Remember
Examples
Fungal Media
Sabouraud agar
Sabouraud agar is used to culture fungi and
has a low pH that inhibits the growth of most
bacteria; also contains the antibiotic gentamicin
to specifically inhibit the growth of Gramnegative bacteria.
Hay infusion agar
Specific for the culturing of slime molds
(though not technically fungi).
Potato dextrose agar
PDA is used to culture of certain types of fungi.
Preparation of CM
Nutrient Broth
Dissolve 8 g of NB powder in 1000 ml of distilled
water in an Erlenmeyer flask. Mix and heat over
the magnetic stirrer until the medium becomes
clear or transparent.
Dispense 8 ml of the medium into sterile test tubes.
Immediately stopper the tubes completely.
Sterilize in the autoclave at 15 psi, 121 C for 15
mins.
Preparation of CM
Nutrient Agar
Dissolve
28 g of NA powder in 1000 l of
distilled water in an Erlenmeyer flask. Mix and
heat over the magnetic stirrer until the
medium becomes clear or transparent.
Sterilize in the autoclave at 15 psi, 121 C for
15 mins.
Dispensing the CM
NA plate
Lay
NA slant
Dispense
NA deep or butt
Dispense
Materials
petri dishes
test tubes
bunsen burners/alcohol lamps
wire inoculating loops
bottles of staining reagents
incubators
Importance of Using
Sterile Technique
Necessary to exclude all microorganisms from
a particular area, so that area will be sterile.
Media should remain sterile before inoculation.
Contaminants- unwanted microorganisms
Contaminatedif the sample contains
contaminants
Inoculating the NB
FINISHED!