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Introduction to cells
Hemocytometer
(or counting chamber)
A specimen slide which is
used to determine the
concentration of cells in a
liquid sample, frequently
used to determine the
concentration of blood cells
a grid (an arrangement of
squares of different sizes) is
etched into the glass of the
hemocytometerallows for
an easy counting of cells.
Loading the
hemocytometer
Both the hemocytometer &
its coverslip should be clean.
Then place the pipette tip
with your sample into one of
the V-shaped wells & gently
expel the sample. The area
under the coverslip fills by
capillary action.
You can load two samples on
one hemocytometer
Counting the cells
Cells that are on the
line of a grid require
special attention. Cells
that touch the top and
right lines of a square
should not be counted,
cells on the bottom
and left side should be
counted using a
microscope.
To distinguish b/w dead &
viable cells