In vitro propagation of Curcuma longa (Turmeric)

INTRODUCTION
Gomathy1, M. Anbazhagan2* and K. Arumugam2 Results and
Turmeric (Curcuma longa
Linn. Syn. C. Tamil Nadu,India
Discussion
domesticaValet.) is one of Explants Selection and Shoot Induction
the important spicecrops. It Rhizomatous buds were found
is a rhizomatous perennial best explants for shoot induction
belonging to family on MS medium supplemented
Zingiberaceae(1). with different concentrations of
As a spice, turmeric is used
Materials and Methods BAP like 0.5, 1.0, 1.5, 2.0, 2.5,
for its colouring properties Curcuma longa and 3.0mg/l or Kn like 0.5, 1.0,
and aroma. Important Medium MS supplemented with IAA, IBA 2.0, 3.0mg/l individually. The
constituents of turmeric and NAA. more number of shoot induction
rhizomes arecurcumin (2-8%) Suplements with Cytokinin and Auxin was observed in two explants
and volatile oil (5-6%) Agar studied on MS medium
(2,4,5). supplemented with BAP 2.0mg/l +
Advent of in vitro techniques IAA 0.5mg/l.
has opened new avenues in Root Induction
improvement of crop The micro cuttingo f in vitro
plants.Tissue culture proliferated shoots were
techniques have been Conclusion implanted on half strength MS
References
standardized for many The more number of shoot medium supplemented
1. Shirgurkar, M.V., C.K.
horticultural crops. The in induction was observed in individually with IAA, IBA and NAA
John and R.S. Nadgauda.
vitropropagation of turmeric two explants studied on MS concentration of 1.0, 2.0, and
2001. Factors affecting in
was first attempted by [6] medium supplemented 3.0mg/l for root initiation. Among
vitro microrhizome
with BAP 2.0mg/l + IAA them, maximum number of root
production in turmeric.
0.5mg/l. formation (95%) was observed in
Plant Cell, Tissue and
the synergistic effect of medium
Organ Culture.64: 5-11.
both BAP + IAA at Using supplementedstandard
2. Panda, M.K., S. Mohanty,
with(1.0mg/l)IBA
E. Subudhi, L. Acharya and concentration 2.0 and
0.5mg/l respectively, were (12error.100.80
S. Nayak. 2007.
found best in regenerating Acclimatization of
Assessment of genetic
shoot tip explants. regenerated plants
stability of
The plantlets were taken out from
micropropagated plants of
the culture tubes when the roots
Curcuma longa by
were partially brown in colour and
cytophotometry and RAPD
root portion was washed with tap
analyses. International
water to remove the attached
Journal of Integrative
medium. Then they were
Biology, 1(3): 189-195.
transferred to pots containing