PRP THERAPY : A MODERN

MEDICINE BOON FOR REPAIR,

RESTORATION AND
REJUVENATION
Dr. Suryakant Hayatnagarkar

Cryo Cell India Pvt. Ltd.

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 PLATELETS
The ubiquitous tiny blood cells derived
from megakaryocytes in bone marrow
2-3 microns in size, devoid of
nucleus,DNA
Contain dense granules and alpha granules

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 PLATELETS

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 PLATELETS

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 PLATELET DERIVED FACTORS
Tissue regeneration
Angiogenesis
Epithelialization
Proliferation and differentiation of osteoblasts
Synthesis of collagen
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 PLATELET DERIVED GROWTH
FACTORS
EGF Epidermal growth factor
PDGF Platelet derived growth factor
IGF Insulin like growth factor
VEGF Vascular endothelial growth f
TGF Transformative growth factor
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 PLATELET DERIVED GROWTH
FACTORS
KGF Keratinocyte growth factor
FGF Fibroblast growth factor
CTGF Connective tissue growth factor
TNF alpha Tumor necrosis factor
Various Interleukins
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 The Primary Growth Factors Role
in Soft Tissue Healing and Repair
PDGF
2 polypeptide chains
AB, BB and AA types (AB in human platelets)
Stored in alpha granules in platelets
TGF beta
Also polypeptide, multiple chains, TGF beta-1
most studied form
Found in platelets and bone, cartilage

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 Effect of PDGF on Tissues
Immediate (within 5 minutes)
Second messenger stimulation
Inflammatory response
Early (30min to 4 hours)
M-RNA stimulation, protein synthesis
Chemotaxis (draws cells to the area)
Late (4-24 hours)
Fibroblast mitosis

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 Effect of Platelet Growth
Factors on Tissues
It appears that through a complex series of
biochemical and cellular events, these growth
factors cause a rapid and sustained increase in
the number of fibroblasts in an area and then
through a poorly understood interaction among
factors modulates cellular activity to cause stem
cell maturation.

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 PRP THERAPY
What do these Growth Factors Do??
Chemotaxis
Directional movement in response to a chemical stimulus
Stems cells are attracted to the growth factors and migrate
into the area
Cell proliferation
Significant increase in cellular reproduction activity
Possibly even a systemc effect
Performance enhancing?

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 The most
natural
injectable

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 METHOD OF PREPARING PRP

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 USE OF PRP IN VARIOUS
DISCIPLINES
PRP therapy finds usage in most
of the medical disciplines and
scientists have used PRP in
treating varied diseases requiring
rapid repair and restoration.
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 USE OF PRP IN THIN
ENDOMETRIUM
Proper endometrium is one of the
main factors for pregnancy in assisted
reproductive technology treatments.
Estrogens, low dose aspirin, G-CSF
have been variously tried.

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 USE OF PRP IN THIN
ENDOMETRIUM
Intrauterine infusion of PRP is
used in endometrium with view
that the Growth factors provided
by platelets will help in restoring
deficient endometrial function.
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 METHOD OF USING PRP IN
ENDOMETRIUM
PRP is prepared from 15 ml blood
using double centrifugation and
final preparation of 0.5 ml is
infused on 10th and additionally
13th day of cycle.
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 SELECTION OF PATIENTS
Patients can be selected on
criteria of previous cycles
showing endometrial
development of less than 7 mm
even after other therapies.
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 METHOD OF USING PRP IN
REPEATED IMPLANTATION
FAILURE
Leila Nazari et al 2016
International journal of
reproductive Biomedicine

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 METHOD OF USING PRP IN
ENDOMETRIUM
Chronic endometritis and
endometriosis cause inflammatory
process. PRP gives anti-inflammatory
effect and cell proliferation effect.
PRP increases progesterone receptor
activity.
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 METHOD OF USING PRP IN
Ovaries
Early ovarian failure is baffling
problem which is not treatable
easily. Early menopause with
increased anti-mullerian activity is a
challenge in infertility management
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 METHOD OF USING PRP IN
Ovaries
PRP application in ovarian soft tissue
aims at cellular growth and
proliferation in ovaries. Published
series of 8 peri-menopausal women
showed return of ovarian activity in
three months.
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 METHOD OF USING PRP IN
Ovaries
Based on known and predictable
endothelial and vascular growth
activity with PRP application and
stimulation of stem cells results are
expected even in recent menopausal
ovaries.
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 METHOD OF USING PRP IN
Ovaries
Scientific evidence is still lacking but
can be gained by using PRP in large
group of patients and best group of
patients to select are those where
laparoscopy is indicated for any other
reason.
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 METHOD OF USING PRP IN
Ovaries
We can do a scientific study in all
those cases of laparoscopy and
prepare PRP before laparscopy to
inject during ovarin observation using
follicle aspiration needles used in IVF
for harvesting ova.
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 Thank You!

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