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Medical Laboratory Machines: Atomic Absorption Spectrophotometer for Biochemistry and Rotary Microtome for Histology Presented by :
Medical Laboratory Machines:
Atomic Absorption
Spectrophotometer for
Biochemistry and Rotary
Microtome for Histology
Presented by :
Katelan Letren, Celine Changa, Crystal Debisingh,
Carlene Celestine, Kayla Gibbs, Zaeanne Joseph,
Helayne Ramoutar
What is the Atomic Absorption Spectrophotometer  Atomic-absorption (AA) spectroscopy uses the absorption of light to
What is the Atomic Absorption
Spectrophotometer
Atomic-absorption (AA) spectroscopy uses the
absorption of light to measure the concentration of gas-
phase atoms in chemistry analysis.
Samples are usually liquids or solids.
The analyte atoms or ions must be vaporized in a flame
or graphite furnace.
The analyte concentration is determined from the
amount of absorption.
Atomic Absorption Spectrophotometer
Atomic Absorption Spectrophotometer
Atomic Absorption Spectrophotometer
Atomic Absorption Spectrophotometer
Atomic Absorption Spectrophotometer Five main Components: 1.The light source 2. An "absorption cell” 3. A monochromator
Atomic Absorption Spectrophotometer
Five main Components:
1.The light source
2.
An "absorption cell”
3.
A monochromator
4.
A detector
5.
A display
MAINTENANCE OF ATOMIC ABSORPTION SPECTROPHOTOMETER Regular maintenance of instruments in any analytical laboratory must be on
MAINTENANCE OF ATOMIC ABSORPTION
SPECTROPHOTOMETER
Regular maintenance of instruments in any analytical
laboratory must be on a regular schedule
Four main areas of maintenance for the atomic absorption
spectrophotometers include:
General instrument maintenance
Gas supply maintenance
Flame component maintenance
Furnace component maintenance
MAINTENANCE OF ATOMIC ABSORPTION SPECTROPHOTOMETER Benefits of routine maintenance:  Instrument lifetime increased  Reduced lifetime
MAINTENANCE OF ATOMIC ABSORPTION
SPECTROPHOTOMETER
Benefits of routine maintenance:
Instrument lifetime increased
Reduced lifetime
Overall improvement on instrument performance
The overall integrity of the atomic absorption
spectrophotometer should be maintained regularly to
gain greater confidence in the analytical results
Care of Atomic Absorption Spectrophotometer  Dust and condensed vapors can accumulate on the case while
Care of Atomic Absorption
Spectrophotometer
Dust and condensed vapors can accumulate on the case while corrosive
liquids may spill on the instrument. However, to minimize damage, wipe
off the machine with a damp, soft cloth using water or a mild detergent
solution. Never use an organic solvent.
Clean the sample compartment windows and lamp with a soft tissue
moistened with a methanol or ethanol and water solution if they
accumulate dust particles or fingerprints. Noisy lamp signals and non-
reproducible analytic results are signs that the windows are not clean.
The remaining optical components are sealed, but they should not be
exposed to corrosive vapors or a dusty atmosphere. In laboratories
where high concentrations of dust or vapors are unavoidable, schedule a
yearly check by a service engineer to maintain the efficiency of optical
light transmission in the instrument. There is no need for an operator to
clean the sealed optical components.
What is a Rotary Microtome?  Microtomes are machines that cut extremely thin sections from a
What is a Rotary Microtome?
Microtomes are machines that cut extremely thin sections
from a sample for applications in histology or pathology. The
sample is analyzed using transmitted electron microscopy
(TEM) or light microscopy.
A microtome consists of a blade holding unit with a blade
holder and clamped blade, the object clamp, an advancing
mechanism, and a mechanism for adjusting section thickness.
The sample is cut upon the rotary action of a hand wheel
which advances the sample towards a blade. Rotary
microtomes are used with hard materials, such as samples
embedded in resins or paraffin. The thickness for manual
operation ranges from 0.5.µm to 60µm.
Rotary Microtome
Rotary Microtome
Steps on Using a Rotary Microtome A. Preparation of the Microtome B. Mounting the Block C.
Steps on Using a Rotary Microtome
A.
Preparation of the Microtome
B.
Mounting the Block
C.
Blade Preparation
D.
Sectioning
E.
Finished
Maintenance of the Rotary Microtome Warnings / Precautions:  Always remove the knife/blade before detaching the
Maintenance of the Rotary
Microtome
Warnings / Precautions:
Always remove the knife/blade before detaching the knife holder from
the instrument.
Always put the knives back into the knife case when not in use
Never place a knife anywhere with the cutting edge facing upwards and
never try to catch a falling knife
When using cleaners, comply with the safety instructions of the
manufacturer and the labor-safety regulations of your laboratory
When cleaning the outer surfaces, do not use xylene, scouring powders or
solvents containing acetone or xylene. Xylene or acetone will damage the
finished surfaces
Ensure that liquids do not enter the interior of the instrument during
cleaning
Maintenance of the Rotary Microtome A. Preparatory steps to follow before cleaning B. Instrument and outside
Maintenance of the Rotary
Microtome
A.
Preparatory steps to follow before cleaning
B.
Instrument and outside surface cleaning
C.
Cleaning the knife: Always wipe the knife from the back
of the knife to the cutting edge. NEVER wipe in the
opposite direction to avoid risk of injury.
D.
Universal cassette lamp cleaning
E.
Lubricating the instrument
Care of the Rotary Microtome 1. Lock wheels and guard and remove the blade from the
Care of the Rotary Microtome
1. Lock wheels and guard and remove the blade from the
blade holder
A. Use forceps or a magnet (NEVER FINGERS) to pick
up the blade.
B. Blades must be stored appropriately
2.
Remove the remaining tissue block
3.
Clean section waste tray with a dry brush
4.
Use appropriate cleaning reagents
5.
Proper disposal
6.
Make sure area is clean and free of sharp objects.
Videos of each machine AAS  http://www.youtube.com/watch?v=_KZjb9G3hB8  https://www.youtube.com/watch?v=HBegTB_WDxQ Rotary microtome  https://www.youtube.com/watch?v=KnMdSgd5mts  https://www.youtube.com/watch?v=ml4fBEmH8Sg
Videos of each machine
AAS
http://www.youtube.com/watch?v=_KZjb9G3hB8
https://www.youtube.com/watch?v=HBegTB_WDxQ
Rotary microtome
https://www.youtube.com/watch?v=KnMdSgd5mts
https://www.youtube.com/watch?v=ml4fBEmH8Sg