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The cell culture is called a primary culture until it is subcultured for the
first time, after which it becomes a secondary culture.
The subsequent cell cultures are known as cell lines.
Since the primary cell culture is heterogenous, we go for selection or
cloning of cells for obtaining particular cells.
Cell cloning
Dilution cloning
Selective media
Flow chart for dilution cloning
Monolayer of cells
Remove the medium and add trypsin
(After few minutes the cells are in suspension)
Add medium to the cells
Count cells
Dilute cells to 10-100 cells/ml
Seed cells in a multi-well dish
Let the cells settle down again
Grow up clones for characterization
Select clone
Selective media
Cell separation
3. Fish cells have been utilised for determining karyotypes and other
aspects of cytogenetics such as chromosomal polymorphism and
speciation, chromosomal abnormalities and evolution.
8. Both primary cultures and established cell lines are also sensitive
and can be used in assay systems for screening aquatic pollutants
for cytotoxicity.
9. Fish cell cultures have been utilized for more detailed
investigations of the processes leading to the proliferation
and differentiation of tumours and tumour cells.
Fish cell cultures are also used for testing and evaluating
the effects of carcinogens such as the use of primary
cultures of fish hepatocytes for investigating carcinogenic
effects of dimethylnitrosamine, aflatoxin B1,
benzo(a)pyrene, and N-methyl-N-nitro-N-
nitrosoguanidine.