Chemical & Biological Investigations of

Oroxylum indicum L.
(Family- Bignoniaceae)
ID: PG14-12-15-013
Department of Pharmacy, State University Of Bangladesh

Introduction Results & Discussion Results & Discussion

Antioxidant Activity Antidepressant Activity

- Oroxylum indicum L. (Family- Bignoniaceae), commonly called midnight 200
Sodium thiosulphate induced sleeping time
Onset time(min)
horror, oroxylum, or Indian trumpet flower and locally called sona pata. Total phenolic content of different extractives

172
Total antioxident capacities of different Fraction 180
14.00 Sleeping time(min)

- Leaves are used as stomachic, carminative and flatulent. Leaf decoction is 160

134.33
11.76
12.00 140

116.33
given in treating rheumatic pain, ulcer, constipation, cough, and bronchitis. 10.90
120

Time(min)
140
123.29
- The crude methanolic extract of Oroxylum indicum and its petroleum ether 10.00
8.98
120 100

81.3
mg of GAE / gm of extractives

86
100.98

Antioxidant Level

69.7
100
(PET), carbon tetrachloride (CTC), Di-chloromethane (DCM) and aqueous 8.00
80
80

60

(AQ) soluble fractions of leaf of O. indicum were subjected to different 6.00

60
57.5
40

17.33

16.33
40
pharmacological and biological investigations which include- 20
21.44
10.47
20.92 20

4.00 3.05
0
0
BHT ASCORBIC MESF PESF CTCSF AQSF DCMSF
I(Negetive) II(Positive) III(MESF200) IV(MESF400)
ACID

 Antimicrobial screening. Antidiarrheal activity. 2.00

0.96
1.20
Different Extract Soluble Fraction
Methanol extract

 Brine shrimp lethality bioassay. Hypoglycemic activity. 0.00

CT PE ME DCM AQ
Central Analgesic Activity
 Antioxidant activity. Central analgesic activity. Different extractives and standard

326.4
350

Peripheral analgesic activity.

Comparison of % time elongation of crude extract
 Thrombolytic activity.
Anti-depressant activity.
300

 Membrane stabilizing activity. Brine Shrimp Lethality Bioassay

234.21
250

198.4
200

% elongation
LC50 values of the different extractives
60 STD

121.79
Aims of the study 150

118.7
54.954 MESF 1

87.35
MESF 2
50 100

71.4

41.69
30.88
50

8.89
3.07

2.73
40
LC50 values

0
00 min 30 min 60 min 90 min

To study the acute toxicity studies for both plant extracts. 30 STD & Crude Extract

To evaluate the in-vitro and in-vivo antioxidant activity.

 To evaluate the gastro protective, antioxidant and 20
Peripheral Analgesic Activity
anti- diabetic activities.
10
Statistical analysis of pharmacological results.
0.454 0.007 0.481 0.627 0.34 %inhibition of writhing
0
VS MESF PESF CTCSF DCMSF AQSF 50 45.61
different extractives
45

40

% of Inhibition of writhing
Plant preview Thrombolytic Activity
35

30
24.56
26.32

25

20

80.00 15

10
% of thrombolysis of different extracts
Kingdom: Plantae 70.00
66.98
5

Class: Magnoliophyta 60.00

STD
MESF 1
MESF 2

Order: Lamiales STD & Crude Extract

50.00
Family: Bignoniaceae
Genus: Oroxylum 40.00 Hypoglycemic Activity
% of clot lysis

Species: O. indicum 28.55

40 Plasma level of glucose of different groups of mice at different times
30.00
35

24.42 35
22.30

20.00
30
25.1
Blood glucose Level

25
21.8

10.00

4.09
17.5

20
1.86 1.78 CTL
STD
0.00
13.2

15
11.9
11.8

MESF PESF CTCSF DCMSF AQSF Blank SK MESF 1

10.1

MESF 2
9.3

8.9

10 7.3
Different extratives and standard
5.7
4.6

4.2
3.8

5 3.1
2.6
3

1.9
2

0
0min 25 min 60 min 120 min 180 min

Membrane Stabilizing Activity Time (Minutes)

Anti-diarrheal Activity Assay

% Inhibition of Haemolysis (Hypotonic Solution Induced) % inhibition of haemolysis (Heat-induced)
Anti-diarrheal effect of methanolic extract
250 242.32

90 88.35 78.26
84.15

80 80.00
60.87
200
68.78 70.00
% Reduction of diarrhea

70
60.00
% of inhibition
% of inhibition

50.00
60 150 132.85 30.43
49.62 40.00

50 103.51
30.00
36.31
100 20.00
40 31.34
10.00
60.93
30 0.00
43.16 STD MESF 200mg MESF 400mg
50
20 26.85 Std & Methanolic extract

10
0
ME PESF CTCSF DCMSF AQSF ASA
0
ME PESF

Different extratives and standard

CTCSF DCMSF AQSF ASA
Different extratives and standard Conclusions
Leaves of a young plant The crude methanolic extract of O. indicum and its different partitionates
exhibited mild to moderate -antioxidant, antimicrobial, membrane
Antimicrobial Screening stabilizing and thrombolytic activities. On the other hand the methanolic
Di-chloromethane soluble fraction (DCMSF) and carbon tetrachloride extracts of leaves of O. indicum and its different partitionates showed
potent lethality in brine shrimp lethality bioassay. In addition, the
soluble fraction (CTCSF) exhibited the inhibition against microbial growth
methanolic extract showed statistically highly significant activities in case
having zone of inhibition. of anti-diarrheal, peripheral analgesic and central analgesic activities.

This is only a preliminary study and to make final comment the extract
should be thoroughly investigated phytochemically and pharmacologically
to exploit their medicinal and pharmaceutical potentialities.