Protein Crystallization and

Structure Determination
Our understanding of life at the molecular
level is highly dependent on the ability to
map the molecular details of individual
proteins and nucleic acids as well as their
interactions with each other and with small
molecules (inhibitors, cofactors,
substrates, etc.). The determination of 3-D
structures of proteins is crucial for the
understanding of these interactions as well
as their structure–function relationships,
which also has many practical applications in
Structure determination is usually a tedious
and expensive process whereby the target
macromolecule must be produced in
relatively large quantities and purified in
high concentrations. A successful
experiment often requires expert knowledge
of biochemistry, bioinformatics and
molecular biology. Over the past decades,
rapid technical advances in DNA cloning,
protein expression and purification
methodologies have made it generally easy
to obtain enough amount of proteins for their
Besides, numerous innovations and
improvements have been made in solving
protein structures using instrumental
methods such as X-ray crystallography,
nuclear magnetic resonance (NMR)
spectroscopy and electron microscopy (EM).
These efforts together have given rise to
dramatic accumulation of 3-D structural data
in the past few decades (Figure 1). The large
number of protein structures will certainly
yield valuable information to the rules for
elucidating protein folding/unfolding
Figure 1. The growth of total available structures deposited in PDB since 2000.
Creative Biostructure has a team of
scientists who have rich experience in
solving the structure of various protein
targets, such as kinases, polymerases,
chaperones and membrane proteins. Over
the years, we have developed four
unparalleled platforms, including
X-ray crystallography, NMR, EM and
membrane protein screening, to facilitate
your research in structural biology.
Figure 2. Four platforms at Creative Biostructure
By using the most advanced platform apparatus and
techniques, Creative Biostructure promises unbeatable
precision in protein structure services with competitive
prices.

Protein crystallization: Creative Biostructure offers high-

throughput UniCrys™ protein crystallization services with
our state-of-the-art facilities. High-quality protein crystals
can be obtained, which are suitable for X-ray diffraction
studies.
Structure determination: Creative Biostructure employs
various strategies for the structure determination of
macromolecules of your interest. Our scientists are
experienced with phase determination of X-ray diffraction
pattern, NMR spectroscopy and cryo-EM. We promise to
 Thank you !
Excerpt from:
https://www.creative-biostructure.com/protein-crystallization-and-structure-determination_13.ht