Escolar Documentos
Profissional Documentos
Cultura Documentos
• OVERVIEW
• 1. Indications
• 2. Collection of sputum
• 3. Sample transport
• 4. Sample analysis
• a. Physical examination
• b. Microbiological examination
1. Gram stain
2. Culture and sensitivity
3. Examination for acid fast bacilli
a. Zn stain
b. Fluorescent stain
c. Culture on conventional media
d. Commercial automated culture system
e. Molecular methods
4. Examination for other specific organisms
• c. Cytological examination
Indications
Indications:
1. drug susceptibility testing
2. species identification if other than M. tuberculosis suspected
3. sputum smear negative and strong clinical suspicion
Prerequisites:
1. 4% NaOH should be added before inoculation
2. This is because sputum samples are contaminated with normal flora, which grow and digest the
media before MTB can grow 3. 4% NaOH kills this flora
Media used:
1. solid media – LJ media (egg based) or Middle brook (agar based)
2. Liquid media – middle brook, TH9, TH 12
CULTURE
• Advantage:
1. sensitivity 80-85%
2. can detect as low as 10-100 bacteria/ml
• Drawbacks:
1. expensive
2. requires 6 weeks for results