Determination of Vitamin C Levels

Group VI
 1 AIM OF EXPERIMENT

2 APPARATUS & CHEMICALS

3 WORK PROCEDURE

4 OBSERVATION RESULT

5 DATA ANALYSIS

6 DISCUSSION

7 CONCLUSION & SUGGESTION

 AIM OF EXPERIMENT

DETERMINE THE LEVELS OF

VITAMIN C
? COMPARE THE VOLUME OF
THIOSULFATE USED IN SAMPLE
WITH VITAMIN C AND SAMPLE
WITHOUT VITAMIN C
 APPARATUS AND CHEMICAL

apparatus

apparatus
 CHEMICALS

VITAMIN C AMYLUM INDICATOR KCO3

H2SO4 2 N I2 0,1 N
Na2S2O3 0,1 N TISSUE
H2O LABEL
 WORK PROCEDURE

1. Standardization of thiosulphate

10 ml of KCO3 and 10 ml The solution was titrated The volume of Experiment was
of iod was put into the with Na2S2O3 and starch titrant was repeated for 3
erlenmeyer indicator was added recorded times

2. Sample
H2O was heat and then it Vitamin C was
20 ml of H2O was 50 ml of iod and 5 ml
was cooled in room crushed and its was
added in vitamin C H2SO4 was added into
temperature weighed (0,3 gr)
solution

Experiment was The volume of The solution was titrated

repeated for 3 titrant was with Na2S2O3 and starch
times recorded indicator was added
 WORK PROCEDURE

3. Blanko

20 ml of H2O was 50 ml of iod and 5 ml The solution was titrated with

added into erlenmeyer H2SO4 was added into Na2S2O3 and starch indicator was
solution added

Experiment was repeated The volume of titrant

for 3 times was recorded
 OBSERVATION RESULT
STANDARDIZATION OF
THISULPHATE
N ACTIVITY RESULT
O
SAMPLE
1 10 ml KIO3 + 10 ml I. 12 ml NO ACTIVITY RESULT
iod II. 12, 8 ml 1 0,3 gr of sample + 30 ml aqu I. 0,3151 gr BLANK
III. 12, 8 ml adest II. 0, 3063 gr
III. 0,3008 gr NO ACTIVITY RESULT
2 Solution + 5 ml H2SO4 2 N I. Yellow 1 20. ml aquadest I. 20 ml
II. Yellow II. 20 ml
III. Yellow III. 20 ml
3 Solution + 50 ml I2 0,1 N I. Brown 2 Solution + 5 ml H2SO4 I. Colorless
II. Brown 2N II. Colorless
III. Brown III. Colorless
4 Titrated with Na2S2O3 0,1 N I. 45,0 ml 3 This PowerPoint
Solution + 50 ml I2Template
0,1 I. Brown
and add amylum indicator II. 41,8 ml has clean and neutral design
N II. Brown
III. 45,0 ml that can be adapted to anyBrown
III.
4 Titrated with Na2S2O3 I.
content and meets various50,5 ml
0,1 N and add amylum II. 51,5 ml
market segments.
indicator III. 51,5 ml
 DATA ANALYSIS
Sample (Vitamin C) and Blanko with Strach
Known : N Na2SO3 = 0,1N
45 ml+ 41.8 ml+ 45 ml
V Na2SO3 sampel =
3
= 43,93 ml
50,5 ml+51 ml+51 ml
V Na2SO3 blanko =
3
= 50,83 ml
Mr C6H8O6 = 176 mg/mmol

Asked: Content of vitamin C?

Answer :
Mass vitamin C
m = (N . V ) thiosulfate x BE
𝑀𝑟
= (0.1 meq/ml x 1ml) x
𝑒𝑞𝑖𝑣𝑎𝑙𝑒𝑛
176 𝑚𝑔/𝑚𝑚𝑜𝑙
= (0.1 meq/ml x 1ml) x
2 𝑚𝑒𝑞/𝑚𝑚𝑜𝑙
= 8.8 mg

So, 1 mL of Na2SO3 0.1 N is equivalent to 8.8 mg of vitamin C. So t

he amount of vitamin C (mg) in 1 mL thiosulfate is 8.8 mg/mL
Mass of experiment vitamin C
𝑉𝑏𝑙𝑎𝑛𝑘𝑜−𝑉 𝑠𝑎𝑚𝑝𝑒𝑙 𝑥 𝑚𝑔 𝑣𝑖𝑡𝑎𝑚𝑖𝑛 𝐶
Mass =
1 𝑚𝐿
38.30 𝑚𝐿−31.93 𝑚𝐿 𝑥 8.8 𝑚𝑔/𝑚𝐿
=
1 𝑚𝐿
= 56.056 mg
Content of vitamin C
𝑚𝑎𝑠𝑠 𝑜𝑓 𝑒𝑥𝑝𝑒𝑟𝑖𝑚𝑒𝑛𝑡
%= x 100%
𝑚𝑎𝑠𝑠 𝑜𝑓 𝑡ℎ𝑒𝑜𝑟𝑦
56.056 𝑚𝑔
= x 100%
300 𝑚𝑔
= 18.68 %
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