The introduction to CS-400

1.Brief Introduction

2.Working principle

3.Composition of instrument
1. Brief Introduction
Some main technical date :
Instrument structure: Discrete system
Throughput: Constant speed, 400 tests/ hour (800 tests/ hour with ISE)
Sample volume:2 to 35μl（Stepping 0.1μl）
Reagent volume: 20 to 350μl，（Stepping 1μl）
Reaction solution volume:150～450μl
115 sample positions, double disks with total 90 reagent position
Integration of sample probe and reagent probe with touch sensor and
sample probe block test function.
 20W /12V Long life quartz halogen lamp (water cooling)
120 pcs of reusable rigid optical plastic reaction cuvette.
Reaction cuvette optical diameter: 6mm
LIS/HIS interface, supports remote maintenance
2.Working principle
Beer-Lambert law

I0
A  lg （1/T ） lg （ ）  b c
It
A －Absorbency of the light when passing through
liquid
T －Transmitted intensity and incident intensity
ratio: transmittance It/I0；
I0 － Incident intensity
It － Transmitted intensity
ε － Molar absorption coefficient of solution
（ml×mmol－1×cm－1）；
c － Mol concentration of the solution（mmol/ml）；
b － Solution layer thickness（cm）；
 Abs
(1) 1-point Assay Blank
（B1+B2+B3）/3 R2～R4

R1

S A L  A L 1
2

SB B1 B2 B3

AL  AL  1
Time

Calculation of absorbance AX 
2
Calculation of concentration C X  K   AX  B  C1  IFA  IFB
Analytical items
TP, ALB, etc.
SB: Stopped cup blank
B1~B3: Passed cup blank
R1~R4: Reagent adding position
Cx: Concentration of standby sample
C1: Concentration of standard 1 solution(reagent blank)
K: Factor
B: Absorbance of blank
IFA and IFB: Instrument constants, representing slope and
intercept
 (2) 2-point Assay

Calculation of absorbance
Calculation of concentration
Analytical items
CRE, etc.
( AM  AM 1 )  k ( AL  AL 1 )
AX 
2
CX  K   AX  B   C1 IFA  IFB
SB: Stopped cup blank
R1~R3: Passed cup blank
R1~R4: Reagent adding position
Ax: the deference between the photometric point M and L
Cx concentration of standby sample
C1: Concentration of standard 1 solution(reagent blank)
K: Factor
B: Absorbance of blank
IFA and IFB: Instrument constants, representing slope and
intercept
 (3) 2-point Rate Assay

Calculation of absorbance:

Calculation of concentration CX  K   AX  B   C1 IFA  IFB

SB: Stopped cup blank
R1~R3: Passed cup blank
R1~R4: Reagent adding position
Ax: Average change in absorbance per minute between
measurement points L and M
Cx concentration of standby sample
C1: Concentration of standard 1 solution(reagent blank)
K: Factor
B: Absorbance of blank
IFA and IFB: Instrument constants, representing slope and
intercept
 (4) Rate A Assay

△A(L-m): Change in absorbance per minute between measurement point L and M

Calculation of absorbance:

Calculation of concentration CX  K   AX  B   C1 IFA  IFB

SB: Stopped cup blank
R1~R3: Passed cup blank
R1~R4: Reagent adding position
Cx concentration of standby sample
C1: Concentration of standard 1 solution(reagent blank)
K: Factor
B: Absorbance of blank
IFA and IFB: Instrument constants, representing slope and
intercept
 Calibration Method
Linearity Method ( 2-point linearity )
S1ABS (B): Absorbance or change in
absorbance per minute of blank (standard 1)
K: Calculated from measured values and
input values of blank (standard 1) and
standard sample (standard2)
C1: Concentration of standard 1（reagent
blank)
C2: Concentration of standard 2
A2: Absorbance or change in absorbance
per minute of standard 2.

C 2  C1
K
A2  B
Calculation of concentration

C X  K   AX  B  C1  IFA  IFB
C 2  C1
K
A2  B
Cx: Concentration of standby sample
AX: Absorbance or change in absorbance per minute
IFA and IFB: Instrument constants, representing slope and
intercept.
Applicable assay
1-point assay, 2-point rate assay, 2-point assay, 3-point assay, 1-
point&rate assay, rate A assay and rate B assay
3.Composition of instrument
1.Analytical unit
switches
(not including
refrigeration power
supply)
2. refrigeration power
supply indicator
（green）
3. main power supply
（breaker）
4.power indicator（red）

12 3 4
1 Power entrance
2 RS232 interface
3 cooling fan
4 light liquid outlet
5 purified water entrance
6 concentrated liquid waste
outlet
7 concentrated waste liquid
level sensor interface
Reaction Unit
Reaction cup：120，
optical path: 6mm
20×6 sets hard optical
plastic
cup
Incubation bath
Digital liquid sensor
7-stop 11-step rinsing of
colorimetric cup
 B F G F
C D A B A B E
Reagent Unit
Reagent position：45×2
R1disk：only for R1and R4
R2disk：only for R2 and R3
Position 45 of two reagent
disk:
Only for phosphor-free
cleaning liquid
Reagent bottle volume：
70mland 20ml
Probe mechanism：
1sample probe, 60nm polishing
treatment probe，Digital liquid
level detection,intelligentized
clot detection and collision
protection
2 reagent probes
High-precision digital liquid
detector
Stirring mechanism：2
High-speed hollow cup motor
Surface high-intensity Teflon
coating
115 sample positions，
including：
Routine sample position： 50
STAT sample position ： 20
Standard solution position: 34
QC position： 8
Cleaning liquid position： 3
Barcode window:1
Supper high precision syringe
with long life ceram piston.
Guaranteed one million times
usage
Electrical board
1. Reaction disk
Circuit board
2. Sample disk
Circuit board
3. Reagent 1 disk
Circuit board
4. Reagent 2disk
Circuit board
5. ISE
Circuit board
6. Main controller
Board
Switching Power Supply Box：
Order: from left to right
＋12V，－12V（simulation）
＋12V（lamp）
＋5V（digital circuit）
＋24V（motor, valve）
1.Interface board

2. Solid relay board

Semiconductor refrigeration systems:：
Control panel (with status indication)
Cooler (with 4 fans）
Cooler display:
E1 refrigeration temperature,
E2 machine internal temperature
C1－C4 4 Cooler current
AC circuit breaker: （5A）
Fan (4A)
4 communication pump (6A)
2 Heater (10A)
4 Switching Power Supply (10A)
Refrigeration system (10A)
Isolating transformers
Arrester panel
Thank you !