Viruses are very small (submicroscopic) infectious particles (virions) They carry genetic information encoded in their nucleic acid, which typically specifies two or more proteins. Viroids are infectious RNA molecules that cause diseases in various plants.
Viruses are very small (submicroscopic) infectious particles (virions) They carry genetic information encoded in their nucleic acid, which typically specifies two or more proteins. Viroids are infectious RNA molecules that cause diseases in various plants.
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Viruses are very small (submicroscopic) infectious particles (virions) They carry genetic information encoded in their nucleic acid, which typically specifies two or more proteins. Viroids are infectious RNA molecules that cause diseases in various plants.
Direitos autorais:
Attribution Non-Commercial (BY-NC)
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Baixe no formato PPTX, PDF, TXT ou leia online no Scribd
Viruses are very small (submicroscopic) infectious
particles (virions) composed of a protein coat and a nucleic acid core. They carry genetic information encoded in their nucleic acid, which typically specifies two or more proteins. Translation of the genome (to produce proteins) or transcription and replication (to produce more nucleic acid) takes place within the host cell and uses some of the host's biochemical "machinery". Viruses do not capture or store free energy and are not functionally active outside their host. They are therefore parasites (and usually pathogens) but are not usually regarded as genuine microorganisms. ost viruses are restricted to a particular type of host. Some infect bacteria, and are known as bacteriophages, whereas others are known that infect algae, protozoa, fungi (mycoviruses), invertebrates, vertebrates or vascular plants. However, some viruses that are transmitted between vertebrate or plant hosts by feeding insects (vectors) can replicate within both their host and their vector. This web site is mostly concerned with those viruses that infect plants but we also provide some taxonomic and genome information about viruses of fungi, protozoa, vertebrates and invertebrates where these are related to plant viruses. We also provide information about viroids, which are infectious RNA molecules that cause diseases in various plants. Their genomes are much smaller than those of viruses (up to 400 nucleotides of circular single-stranded RNA) and do not code for any proteins. We also provide information about viroids, which are infectious RNA molecules that cause diseases in various plants. Their genomes are much smaller than those of viruses (up to 400 nucleotides of circular single- stranded RNA) and do not code for any proteins. Why are viruses important? Viruses also cause many important plant diseases and are responsible for huge losses in crop production and quality in all parts of the world. Infected plants may show a range of symptoms depending on the disease but often there is leaf yellowing (either of the whole leaf or in a pattern of stripes or blotches), leaf distortion (e.g. curling) and/or other growth distortions (e.g. stunting of the whole plant, abnormalities in flower or fruit formation). R R
The highest level of virus classification recognises six major groups, based on the nature of the genome: Double-stranded DNA (dsDNA) Single-stranded DNA (ssDNA) Reverse-transcribing viruses Double-stranded RNA (dsRNA) Negative sense single-stranded RNA (ssRNA-) Positive sense single-stranded RNA (ssRNA+) Amongst plant viruses, the most frequently encountered shapes are: Isometric Rod-shaped Filamentous Geminate Bacilliform 2sometric: apparently spherical and (depending on the species) from about 18nm in diameter upwards. The example here shows Tobacco necrosis virus, genus Necrovirus with particles 26 nm in diameter. tered shapes are: jod-shaped: about 20-25 nm in diameter and from about 100 to 300 nm long. These appear rigid and often have a clear central canal (depending on the staining method used). Some viruses have two or more different lengths of particle and these contain different genome components. The example here shows Tobacco mosaic virus, genus Tobamovirus with particles 300 nm long. ilamentous: usually about 12 nm in diameter and more flexuous than the rod-shaped particles. They can be up to 1000 nm long, or even longer in some instances. Some viruses have two or more different lengths of particle and these contain different genome components. The example here shows 6otato virus Y, genus 6otyvirus with particles 740 nm long. ueminate: twinned isometric particles about 30 x 18 nm. These particles are diagnostic for viruses in the family ueminiviridae which are widespread in many crops especially in tropical regions. The example here shows Maize streak virus, genus MastrevirusÄ áacilliform: c Ä
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Ä Some important animal and human viruses can be spread through aerosols. The viruses have the "machinery" to enter the animal cells directly by fusing with the cell membrane (e.g. in the nasal lining or gut). plant cells have a robust cell wall and viruses cannot penetrate them unaided. ost plant viruses are therefore transmitted by a vector organism that feeds on the plant or (in some diseases) are introduced through wounds made, for example, during cultural operations (e.g. pruning). A small number of viruses can be transmitted through pollen to the seed (e.g. áarley stripe mosaic virus, genus Hordeivirus) while many that cause systemic infections accumulate in vegetatively-propagated crops. The major vectors of plant viruses are: REPLICATION OF VIRUS Since tobacco mosaic virus (TV) has been studied the most extensively, its reproduction will be briefly described. The replication of virus RNA is an essential part of the reproduction process. ost plants contain RNA-dependent RNA polymerases, and it is possible that these normal constituents replicate the virus RNA. However, some plant virus genomes (e.g., turnip yellow virus and cowpea mosaic virus) appear to be copied by a virus-specific RNA replicase. Possibly TV RNA is also replicated by a viral RNA polymerase, but the evidence is not clear on this matter. Four TV-specific proteins, one of them the coat protein, are known to be made. After the coat protein and RNA genome have been synthesized, they spontaneously assemble into complete TV virions in a highly organized process. O !O Ä !O
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Ä 2nsects. This forms the largest and most significant vector group and particularly includes: Aphids: transmit viruses from many different genera, including 6otyvirus, Cucumovirus and Luteovirus.The picture shows the green peach aphid Myzus persicae, the vector of many plant viruses, including 6otato virus Y. (Figure from Nuessly & Webb, Insect anagement for Leafy Vegetables, ENY-475, September 2003, University of Florida, Institute of Food and Agricultural Sciences (UF/IFAS)).
Whiteflies: transmit viruses from several genera but particularly those
in the genus áegomovirus. The picture shows áemisia tabaci, the vector of many viruses including Tomato yellow leaf curl virus and Lettuce infectious yellows virus.
!oppers: transmit viruses from several genera, including those in the
families j abdoviridae and jeoviridae. The picture shows Micrutalis malleifera, the treehopper vector of Tomato pseudo-curly top virus.
Thrips: transmit viruses in the genus Tospovirus. The picture shows
Frankinella occidentalis, the western flower thrips that is a major vector of Tomato spotted wilt virus.
áeetles: transmit viruses from several genera, including Comovirus and
Sobemovirus ematodes: these are root-feeding parasites, some of which transmit viruses in the genera Nepovirus and Tobravirus. The picture shows an adult female of 6aratric odorus pac ydermus, the vector of Tobacco rattle virus. (Figure from Description 398, courtesy of the Scottish Crop Research Station). 6lasmodiophorids: these are root-infecting obligate parasites traditionally regarded as fungi but now known to be more closely related to protists. They transmit viruses in the genera áenyvirus, áymovirus, Furovirus, 6ecluvirus and 6omovirus. The picture shows 6olymyxa graminis, the vector of several cereal viruses including áarley yellow mosaic virus, growing within a barley root cell. ites: these transmit viruses in the genera jymovirus and Tritimovirus. The picture shows Aceria tosic ella, the vector of W eat streak mosaic virus. (bar represents 10 µm). jFjNCS
PRESCOTT·S ICROBIOLOGY (October 2002)
Prescott, Harley and Klein's 5th edition 415-440 PETER ,J. RUSSELL(2000) ´CHROOSOAL BASIC INHERITANCE .In., ¶GENETICS·.BENJAIN/CUINGS IS AN IPRINT OF ADDISON WESLEY LONGAN(FIFTH Edn)PAGE NO.47-55 http://ghr.nlm.nih.gov/handbook/basics/structure of virus replication http://en.wikipedia.org/wiki/virusreplication http://microbiology.suite101.com/article.cfm/plantv irus