DEPARTMENT OF TECHNICAL EDUCATION

ANDHRA PRADESH
Name : J Srinivasa Rao
Designation : Lecturer
Branch : D.A.E.I.E
Institute : Govt. Polytechnic,Kothagudem
Year/Semester: VI Sem
Subject : Analytical Instrumentation
Subject Code : AEI-603
Topic : Introduction to Analytical
Instrumentation and
spectrophotometers
Duration : 100 Min
Sub Topic : Ultravoilet Spectrophotometer
Teaching Aids : PPT,Pictures, Animations

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 Objective:

On completion of this period you would be able to know

about UV Spectrophotometer

• Principle
• Construction
• Working and
• Applications

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 ULTRAVOILET SPECTROPHOTOMETER
• When a beam of radiant energy strikes the surface of a
substance, the radiation interacts with the atoms and
molecules of the substance.

The radiation may be

• Transmitted
• Absorbed
• Scattered
• Reflected depending upon the properties of the
substance.
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 PRINCIPLE

• It is based upon the principle that the amount of

absorption that occurs is dependent on the number of
molecules present in the material.

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AEI-603.7 to 603.8 5
ANIMATION
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UV Spectrophotometer

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 CONSTRUCTION & WORKING
• The ultraviolet spectrometer consists of a radiant source
of hydrogen or deuterium source.
• The radiation from the source through the condensating
lens is made to fall on the focusing lens through the
entrance slit.
• The radiation from the light source is passed either
directly by means of a lens or mirror into the narrow slit
of the monochromator and allowed to fall on the
dispersing medium, where it gets isolated.

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• Here the dispersing medium is the diffraction grating.
• The isolated radiation is passed through the cuvette
where the sample is held. The radiation may be
absorbed, scattered or reflected by the sample.
• This isolated radiation of a particular wavelength is
selected by a proper filter.

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• This radiation is made to fall upon a photo sensitive
element in which the light energy is converted into
electrical energy.

• The electric current produced can be measured with a

sensitive galvanometer directly or after suitable
amplification.

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 COMPONENTS
1. Radiation Source
2. Monochromator
3. Optical components
4. Detector

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 Radiation source

• The ultraviolet radiation ranges from 200nm to 400nm.

The radiation sources used for ultraviolet region are
hydrogen lamp and deuterium lamp

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 Monochromator

• For better isolation of spectral energy diffraction grating

is used for UV spectrometer.

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 Optical components

• The optical components in the UV spectrophotometer

are entrance slit, exit slit and condensating lens.

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 Detector

• The detectors used for a UV spectrometer are photocells

and photo multiplier tubes.

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 UV Spectroscope Applications

UV spectroscopy is used in the quantitative

determination of solutions of transition metal ions and
highly conjugated organic compounds

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 Summary

In the class we have learnt about

• UV spectrum
• Principals
• Construction works
• Applications

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 Quiz

1. The Light source used for UV spectrophotometer is

(c) Deuterium lamp

(d) LASER
(e) Tungsten Lamp
(f) Globar Rod

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 Quiz

(2)The monochromator used for UV Spectrophotometer is

(c) Prism
(d) Diffraction grating
(e) Slit
(f) All the above

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 Quiz

3) The detector used for UV Spectrophotometer is

(b) Photo multiplier tube
(c) Electrochemical Detector
(d) Flame ionisation detector
(e) None of the above

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Thank you

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