,0 01

Bases Nutricionais de Preparo Rpido -

Chocolate 50 e 70% de Cacau

Base com Zero de Acar, Zero de Lactose, Zero de Glten e Zero de Gorduras Trans

Superinovao: Nova Base Para Preparo Rpido de Chocolate Com 50% de Cacau Base com Zero de Acar, Zero de Lactose, Zero de Glten e Zero de Gorduras Trans Reduo Calrica de 25 a 27% em Relao ao Chocolate Normal e Rica em Fibras

Os Benefcios do Cacau Para a Sade

Reduo da Presso Sangunea, Proteo Endotelial e Melhora da Funo Cognitiva em Idosos
O cacau contm mais antioxidantes fenlicos do que a maioria dos alimentos. Flavonoides, incluindo catequina, epicatequina e procianidinas predominam na atividade antioxidante. A estrutura tricclica dos flavonoides determina os efeitos antioxidantes que eliminam as espcies reativas de oxignio, quela Fe2+ e Cu+, inibe enzimas e regula as defesas antioxidantes (Katz et al., 2011). De acordo com Ried et al. (2012) estudos epidemiolgicos sugerem que os produtos ricos em cacau so capazes de reduzir o risco de doena cardiovascular. Os flavanis encontrados no cacau demonstraram aumentar os nveis de xido ntrico (NO) endotelial, que promovem a vasodilatao e, por seguinte, a reduo da presso sangunea. Um estudo duplo-cego, grupo-paralelo e randomizado, publicado no peridico Hypertension, setembro de 2012, verificou que o consumo regular de flavanis do cacau pode ser eficaz na melhora da funo cognitiva em idosos com transtorno cognitivo leve. Este efeito aparece mediado, em parte, pela melhora da sensibilidade insulina. Este estudo tambm mostrou que alm da funo cognitiva e a resistncia insulina, os pacientes apresentaram reduo da presso arterial e da peroxidao lipdica. Outro estudo realizado pelo Departamento de Cincias Clnicas da Universidade de Chester, Reino Unido, publicado no peridico Diabetic Medicine em outubro de 2012 comprovou que o consumo de altas doses de polifenis do chocolate capaz de proteger contra a disfuno endotelial induzida pela hiperglicemia aguda e o estresse oxidativo em indivduos com diabetes tipo 2. Esta concluso foi baseada nos resultados do estudo realizado em dez indivduos com diabetes tipo 2 que consumiram previamente a uma carga oral de glicose (75 g), chocolate com alta concentrao de polifenis, que foram capazes de melhorar a funo endotelial desses pacientes quando comparados aos pacientes que consumiram chocolates com baixa concentrao de polifenis (1,7 0,1 vs 2,3 0,1%, P = 0,01). Osakabe e Shibata (2012) realizaram um estudo em animais, o qual confirmou os efeitos do consumo do cacau sobre a microcirculao mesentrica de ratos alimentados com uma dieta rica em gordura. Os resultados sugeriram que um dos mecanismos de hipotenso atribudos ao cacau devido melhora da disfuno endotelial em arterolas induzidas por uma dieta inadequada.
Katz DL, Doughty K, Ali A. Cocoa and chocolate in human health and disease. Antioxid Redox Signal. 2011 Nov 15;15(10):2779-811. doi: 10.1089/ars.2010.3697. Epub 2011 Jun 13. Ried K, Sullivan TR, Fakler P, Frank OR, Stocks NP. Effect of cocoa on blood pressure. Cochrane Database Syst Rev. 2012 Aug 15;8:CD008893. Desideri G, Kwik-Uribe C, Grassi D, Necozione S, Ghiadoni L, Mastroiacovo D, Raffaele A, Ferri L, Bocale R, Lechiara MC, Marini C, Ferri C. Benefits in cognitive function, blood pressure, and Direitoscocoa Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so insulin resistance through flavanol consumption in elderly subjects with mild cognitive impairment: the Cocoa, Cognition, and Aging (CoCoA) study. Hypertension. 2012 Sep;60(3):794-801. de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios doi: 10.1161/HYPERTENSIONAHA.112.193060. Epub 2012 Aug 14. eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200. Mellor DD, Madden LA, Smith KA, Kilpatrick ES, Atkin SL. High-polyphenol chocolate reduces endothelial dysfunction and oxidative stress during acute transient hyperglycaemia in Type 2 diabetes: a pilot randomized controlled trial. Diabet Med. 2012 Oct 6. doi: 10.1111/dme.12030. [Epub ahead of print] Osakabe N, Shibata M. Ingestion of cocoa ameliorates endothelial dysfunction in mesentery arterioles induced by high fat diet in rats: An in vivo intravital microscopy study. Life Sci. 2012 Sep 12. pii: S0024-3205(12)00481-X. doi: 10.1016/j.lfs.2012.08.031. [Epub ahead of print]

Doutor, Conhea a Base de Preparo Rpido de Chocolate com 50 e 70% de Cacau

Principais Benefcios, Vantagens e Informaes Nutricionais A base de chocolate rica em cacau apresenta uma srie de benefcios e diferenciais, entre os quais
se destacam: Reduo calrica de 25 a 27% em relao ao chocolate normal; Possui o mnimo possvel de manteiga de cacau que um produto baseado em chocolate pode ter; Zero de gordura trans; Apresenta at 4 vezes mais fibras do que uma barra de cereal; Zero acar, ideal para diabticos; Zero de glten, ideal para celacos; Zero de lactose, ideal para intolerantes lactose.

Informaes Nutricionais Chocolate 50% de Cacau (Poro de 25 gramas)

Quantidade por Poro Valor Energtico 106 Kcal/445 KJ Carboidratos 12 g Dos quais, poliois 8,6 g Outros 3,4 g Protenas 1,1 g Gorduras Totais 7,9 g Gorduras Saturadas 5,0 g Gorduras Trans 0g Fibra Alimentar 4,6 g Sdio 1,5 mg
* Valor de referncia para uma dieta de 2000 Kcal ou 8400 KJ. ** Valores no estabelecidos.

% VD (*) 5 4 ** ** 1 14 23 ** 19 0

Informaes Nutricionais Chocolate 70% de Cacau (Poro de 25 gramas)

Quantidade por Poro % VD (*) Valor Energtico 104 Kcal/437 KJ 5 Carboidratos 11,6 g 4 Dos quais, poliois 5,2 g ** Outros 6,4 g ** Protenas 2,2 g 2 Gorduras Totais 8,1 g 15 Gorduras Saturadas 5,1 g 23 Direitos AutoraisTrans Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas na prtica, e so Gorduras 0 g pelo profissional prescrito antes de adotadas ** de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Fibra Alimentar 4,9 g 20 Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200. Sdio 2,3 mg 0
* Valor de referncia para uma dieta de 2000 Kcal ou 8400 KJ. ** Valores no estabelecidos.

Indicaes Teraputicas Baseadas em Evidncias

Nutracuticos Promotores da Sade Cardiovascular
1. Chocolate Redutor do Risco Cardiovascular Melhora do Perfil Lipdico, Presso Sangunea e Sensibilidade da Insulina
Aumento dos Nveis de HDL e Reduo dos Nveis de LDL-Colesterol

Vitamina C ....................500 mg Vitamina E ..................... 200 UI Coenzima Q10 ............... 60 mg Selnio ....................... 100 mcg Chocolate 50 ou 70% de cacau qsp .................... 1 UN Mande 60 chocolates. Consumir dois chocolates ao dia.

Este estudo avaliou os efeitos da suplementao de vitamina C (500 mg), vitamina E (200 UI), coenzima Q10 (60 mg) e selnio (100 mcg) em pacientes com mltiplos fatores de risco cardiovascular e os resultados confirmaram um aumento significativo da elasticidade das grandes e pequenas artrias, melhora nos nveis glicmicos, no perfil lipdico e na presso sangunea.
Shargorodsky M, Debby O, Matas Z, Zimlichman R. Effect of long-term treatment with antioxidants (vitamin C, vitamin E, coenzyme Q10 and selenium) on arterial compliance, humoral factors and inflammatory markers in patients with multiple cardiovascular risk factors. Nutr Metab (Lond). 2010 Jul 6;7:55.

2. Chocolate Antiagregante Plaquetrio Ideal Para Preveno de Processos Trombticos e Microtrombticos

Alta Capacidade Antioxidante

Oli-Ola .................... 200 mg* Chocolate 50 ou 70% de cacau qsp .................... 1 UN

* Oli-Ola contm 3% de hidroxitirosol, sendo 400 mg correspondente a aproximadamente 12,5 mg de hidroxitirosol.

Mande 60 chocolates. Consumir dois chocolates ao dia, sendo que no primeiro dia podem ser consumidos 4 chocolates.

Um estudo publicado no peridico European Journal of Clinical Nutrition avaliou os efeitos antioxidantes de um extrato de oliva rico em hidroxitirosol administrado junto com o caf-da-manh e comprovou que a administrao de 25 mg/dia hidroxitirosol no primeiro dia e 12,5 mg/dia nos prximos dias promoveu ao antiagregante plaquetria, levando a uma possvel preveno de processos trombticos e microtrombticos.
Lger CL, Carbonneau MA, Michel F, Mas E, Monnier L, Cristol JP, Descomps B. A thromboxane effect of a hydroxytyrosol-rich olive oil wastewater extract in patients with uncomplicated type I diabetes. Eur J Clin Nutr. 2005 May;59(5):727-30.

Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

Indicaes Teraputicas Baseadas em Evidncias

Suplementaes Para Restaurao da Proteo Aumento da Sensibilidade Insulina Endotelial
1. Chocolate Redutor da Presso Arterial em Pacientes Hipertensos
Associada

Magnsio elementar ..... 300 mg Chocolate 50 ou 70% de cacau qsp .....................1 UN Mande 30 chocolates. Consumir um chocolate ao dia.

Os resultados desse estudo sugerem que a suplementao de 300 mg magnsio elementar reduz a presso arterial em pacientes hipertensos e melhora a sensibilidade insulina em pacientes sobrepesados.
Lee S, Park HK, Son SP, Lee CW, Kim IJ, Kim HJ. Effects of oral magnesium supplementation on insulin sensitivity and blood pressure in normo-magnesemic nondiabetic overweight Korean adults. Nutr Metab Cardiovasc Dis. 2009 Dec;19(11):781-8. Epub 2009 Apr 8.

2. Chocolate Restaurador da Funo Endotelial em Idosos

Restaurao Aguda da Funo Endotelial

Vitamina C ................... 500 mg Vitamina E ..................... 300 UI cido alfa-lipoico ........ 300 mg Chocolate 50 ou 70% de cacau qsp .....................1 UN Mande 60 chocolates. Consumir dois chocolates ao dia junto s principais refeies.

Este estudo teve como objetivo avaliar os efeitos da suplementao antioxidante na restaurao da funo endotelial. Participaram do estudo 87 voluntrios saudveis, sendo 42 jovens e 45 idosos, os quais receberam vitamina C 1.000 mg + vitamina E 600 UI + cido alfa-lipoico 600 mg. Os resultados comprovaram que a suplementao antioxidante restaura de forma aguda a funo endotelial nos idosos e sugere que esta disfuno relacionada com a idade atribuda, pelo menos em parte, aos radicais livres.
Wray DW, Nishiyama SK, Harris RA, Zhao J, McDaniel J, Fjeldstad AS, Witman MA, Ives SJ, BarrettO'Keefe Z, Richardson RS. Acute reversal of endothelial dysfunction in the elderly after antioxidant consumption. Hypertension. 2012 Apr;59(4):818-24. Epub 2012 Feb 21.

3. Chocolate Restaurador da Disfuno Endotelial em Portadores de Diabetes Tipo 2

Reduo do Estresse Oxidativo Vascular

Coenzima Q10 ............. 200 mg Chocolate 50 ou 70% de cacau qsp .....................1 UN Mande 30 chocolates. Consumir um chocolate ao dia.

Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser GT, analisadas antes de adotadas na prtica, e so Hamilton SJ, Chew Watts pelo GF. profissional Coenzyme prescrito Q10 improves endothelial dysfunction in statin-treated type de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibidaDiabetes a veiculao para pblico leigo, por meios 2 diabetic patients. Care. 2009 May;32(5):810-2. Epub 2009 Feb 19. eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

Este estudo avaliou os benefcios da suplementao oral de 200 mg de coenzima Q10 na melhora da disfuno endotelial em portadores de diabetes tipo 2 tratados com estatinas. Os resultados demonstraram que a suplementao melhora a disfuno endotelial provavelmente por alterar o estresse oxidativo vascular.

Indicaes Teraputicas Baseadas em Evidncias

Chocolate Pr-Memria e Pr-Cognio Para Pacientes Idosos 1. Chocolate Promotor da Melhora da Funo Cognitiva em Idosos
Ao 100 Vezes Mais Antioxidante Que Todos os Compostos Polifenlicos

BioActive-Quinone (PQQ) ............................. 20 mg Chocolate 50 ou 70% de cacau qsp .................... 1 UN Mande 30 chocolates. Consumir um chocolate ao dia.

Este estudo clnico duplo-cego e placebo-controlado conduzido por pesquisadores japoneses avaliou os efeitos da suplementao de 20 mg ao dia de PQQ em adultos e idosos e comprovou que esta suplementao capaz de melhorar os testes de funo cognitiva em um grupo de adultos de meia idade e idosos.
Nakano M, Ubukata K, Yamamoto T, Yamaguchi H. Effect of pyrroloquinoline quinone (PQQ) on mental status of middleaged and elderly persons. FOOD Style 21. 2009;13(7):50-3.

Legenda. Resultados do teste de memria de curto prazo comprovando a eficcia de PQQ na melhora da funo cognitiva.

Referncias Bibliogrficas
1.Rahman M, Halade GV, Bhattacharya A, Fernandes G. The fat-1 transgene in mice increases antioxidant potential, reduces pro-inflammatory cytokine levels, and enhances PPAR-gamma and SIRT-1 expression on a calorie restricted diet. Oxid Med Cell Longev. 2009 Nov-Dec;2(5):307-16. 2.Shargorodsky M, Debby O, Matas Z, Zimlichman R. Effect of long-term treatment with antioxidants (vitamin C, vitamin E, coenzyme Q10 and selenium) on arterial compliance, humoral factors and inflammatory markers in patients with multiple cardiovascular risk factors. Nutr Metab (Lond). 2010 Jul 6;7:55. 3.Lger CL, Carbonneau MA, Michel F, Mas E, Monnier L, Cristol JP, Descomps B. A thromboxane effect of a hydroxytyrosol-rich olive oil wastewater extract in patients with uncomplicated type I diabetes. Eur J Clin Nutr. 2005 May;59(5):727-30. 4.Lee S, Park HK, Son SP, Lee CW, Kim IJ, Kim HJ. Effects of oral magnesium supplementation on insulin sensitivity and
Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so pressure inexclusivo normo-magnesemic nondiabetic de distribuio e uso de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais overweight Korean adults. Nutr Metab Cardiovasc Dis. 2009 autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

blood

Dec;19(11):781-8. Epub 2009 Apr 8. Fjeldstad AS, Witman

5. Wray DW, Nishiyama SK, Harris RA, Zhao J, McDaniel J, MA, Ives SJ, Barrett-O'Keefe Z, Richardson RS. Acute reversal of endothelial dysfunction in

Abstracts
Antioxid Redox Signal. 2011 Nov 15;15(10):2779-811. doi: 10.1089/ars.2010.3697. Epub 2011 Jun 13. Cocoa and chocolate in human health and disease. Katz DL, Doughty K, Ali A. Source Yale University Prevention Research Center, Griffin Hospital, Derby, Connecticut 06418, USA. david.katz@yale.edu Abstract Cocoa contains more phenolic antioxidants than most foods. Flavonoids, including catechin, epicatechin, and procyanidins predominate in antioxidant activity. The tricyclic structure of the flavonoids determines antioxidant effects that scavenge reactive oxygen species, chelate Fe2+ and Cu+, inhibit enzymes, and upregulate antioxidant defenses. The epicatechin content of cocoa is primarily responsible for its favorable impact on vascular endothelium via its effect on both acute and chronic upregulation of nitric oxide production. Other cardiovascular effects are mediated through anti-inflammatory effects of cocoa polyphenols, and modulated through the activity of NF-B. Antioxidant effects of cocoa may directly influence insulin resistance and, in turn, reduce risk for diabetes. Further, cocoa consumption may stimulate changes in redox-sensitive signaling pathways involved in gene expression and the immune response. Cocoa can protect nerves from injury and inflammation, protect the skin from oxidative damage from UV radiation in topical preparations, and have beneficial effects on satiety, cognitive function, and mood. As cocoa is predominantly consumed as energy-dense chocolate, potential detrimental effects of overconsumption exist, including increased risk of weight gain. Overall, research to date suggests that the benefits of moderate cocoa or dark chocolate consumption likely outweigh the risks. PMID: 21470061 [PubMed - indexed for MEDLINE] Cochrane Database Syst Rev. 2012 Aug 15;8:CD008893. Effect of cocoa on blood pressure. Ried K, Sullivan TR, Fakler P, Frank OR, Stocks NP. Source National Institute of IntegrativeMedicine,Melbourne, karin.ried@adelaide.edu.au.

Australia.

Abstract BACKGROUND: High blood pressure is an important risk factor for cardiovascular disease attributing to about 50% of cardiovascular events worldwide and 37% of cardiovascular related deaths in Western populations. Epidemiological studies suggest that cocoa rich products reduce the risk of cardiovascular disease. Flavanols found in cocoa have been shown to increase the formation of
Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

endothelial nitric oxide which promotes vasodilation and therefore blood pressure reduction. Previous meta-analyses have shown that cocoa-rich foods may reduce blood pressure. Recently additional trials had conflicting results. OBJECTIVES: To determine the effect of flavanol-rich chocolate or cocoa products on blood pressure in people with or without hypertension. SEARCH METHODS: We searched the following electronic databases from inception to November 2011: Cochrane Hypertension Group Specialised Register, CENTRAL, MEDLINE and EMBASE. In addition we searched international trial registries, and the reference lists of review articles and included trials. SELECTION CRITERIA: Randomised controlled trials (RCT) investigating the effects of chocolate or cocoa products on systolic and diastolic blood pressure in adults for a minimum of two weeks duration. DATA COLLECTION AND ANALYSIS: Two authors independently extracted data and assessed the risk of bias in each trial in consultation with a third author. Random effects meta-analyses on all studies fitting the inclusion criteria were conducted using Review Manager version 5.1 and Stata version 12. Heterogeneity was explored by subgroup analyses and univariate meta-regression analysis of several variables including dosage of flavanol content (total or monomers) in chocolate or cocoa products, blinding, baseline blood pressure, theobromine content, sugar content, bodymass-index (BMI), duration and age. MAIN RESULTS: Twenty studies met the inclusion criteria. Meta-analyses of the 20 studies involving 856 mainly healthy participants revealed a statistically significant blood pressure reducing effect of flavanol-rich cocoa products compared with control in short-term trials of 2-18 weeks duration: Mean difference SBP (95%CI): -2.77 (-4.72, -0.82) mm Hg, p=0.005, n=20; mean difference DBP (95%CI): - 2.20 (3.46, -0.93) mm Hg, p=0.006, n=19 available for DBP.Trials provided participants with 30-1080 mg of flavanols (mean=545.5 mg) in 3.6-105 g of cocoa products per day in the active intervention group. In half of the trials (n=10) the active group consumed 500-750 mg of flavanols per day. The control group received either a flavanol-free product (n=12) or a low-flavanol containing cocoa powder (6.4 and 41 mg flavanols, n=8). Subgroup meta-analysis of trials with a flavanol-free control group revealed a significant blood pressure reducing effect, in contrast to trials using a low-flavanol product in the control group. This analysis may have been confounded by trial duration and the level of blinding of participants.Trial duration was short (mean 4.4 weeks, range 2-8 weeks, n=19, and one trial of 18 weeks). A significant blood pressure reducing effect was evident in trials of 2 weeks duration (n=9), but not in trials of >2 weeks duration (n=11). It is important to note that seven out of the nine trials (78%) of 2 weeks duration also had a flavanol-free control group. Therefore, subgroup analysis by duration might be confounded by flavanol dosage used in the control groups, and the level of blinding of participants.Adverse effects including gastrointestinal complaints and distaste of the trial product were reported by 5% of patients in the active cocoa intervention group and 1% of patients in the control groups. AUTHORS' CONCLUSIONS:
Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

Flavanol-rich chocolate and cocoa products may have a small but statistically significant effect in lowering blood pressure by 2-3 mm Hg in the short term.Our findings are limited by the heterogeneity between trials, which was explored by univariate meta-regression and subgroup analyses. Subgroup meta-analysis of trials using a flavanol-free control group revealed a significant blood pressure reducing effect of cocoa, whereas analysis of trials using a low-flavanol control product did not. While it appears that shorter trials of 2 weeks duration were more effective, analysis may be confounded by type of control and unblinding of participants, as the majority of 2-week trials also used a flavanol-free control and unblinding of participants. Results of these and other subgroup analyses based on, for example, age of participants, should be interpreted with caution and need to be confirmed or refuted in trials using direct randomized comparison.Long-term trials investigating the effect of cocoa products are needed to determine whether or not blood pressure is reduced on a chronic basis by daily ingestion of cocoa. Furthermore, long-term trials investigating the effect of cocoa on clinical outcomes are also needed to assess whether cocoa has an effect on cardiovascular events and to assess potential adverse effects associated with chronic ingestion of cocoa products. PMID: 22895979 [PubMed - indexed for MEDLINE] Hypertension. 2012 Sep;60(3):794-801. doi: 10.1161/HYPERTENSIONAHA.112.193060. Epub 2012 Aug 14. Benefits in cognitive function, blood pressure, and insulin resistance through cocoa flavanol consumption in elderly subjects with mild cognitive impairment: the Cocoa, Cognition, and Aging (CoCoA) study. Desideri G, Kwik-Uribe C, Grassi D, Necozione S, Ghiadoni L, Mastroiacovo D, Raffaele A, Ferri L, Bocale R, Lechiara MC, Marini C, Ferri C. Source University of L'Aquila, Department of Life, Health, and Environmental Sciences, Viale S Salvatore, Delta 6 Medicina, 67100 Coppito, L'Aquila, Italy. giovambattista.desideri@cc.univaq.it Abstract Flavanol consumption is favorably associated with cognitive function. We tested the hypothesis that dietary flavanols might improve cognitive function in subjects with mild cognitive impairment. We conducted a double-blind, parallel arm study in 90 elderly individuals with mild cognitive impairment randomized to consume once daily for 8 weeks a drink containing 990 mg (high flavanols), 520 mg (intermediate flavanols), or 45 mg (low flavanols) of cocoa flavanols per day. Cognitive function was assessed by Mini Mental State Examination, Trail Making Test A and B, and verbal fluency test. At the end of the follow-up period, Mini Mental State Examination was similar in the 3 treatment groups (P=0.13). The time required to complete Trail Making Test A and Trail Making Test B was significantly (P<0.05) lower in subjects assigned to high flavanols (38.1010.94 and 104.1028.73 seconds, respectively) and intermediate flavanols (40.2011.35 and 115.9728.35 seconds, respectively) in comparison with those assigned to low flavanols (52.6017.97 and 139.2343.02 seconds, respectively). Similarly, verbal fluency test score was significantly (P<0.05)
Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

better in subjects assigned to high flavanols in comparison with those assigned to low flavanols (27.506.75 versus 22.308.09 words per 60 seconds). Insulin resistance, blood pressure, and lipid peroxidation also decreased among subjects in the high-flavanol and intermediate-flavanol groups. Changes of insulin resistance explained 40% of composite z score variability through the study period (partial r(2)=0.4013; P<0.0001). To the best of our knowledge, this is the first dietary intervention study demonstrating that the regular consumption of cocoa flavanols might be effective in improving cognitive function in elderly subjects with mild cognitive impairment. This effect appears mediated in part by an improvement in insulin sensitivity. PMID: 22892813 [PubMed - in process] Diabet Med. 2012 Oct 6. doi: 10.1111/dme.12030. [Epub ahead of print] High-polyphenol chocolate reduces endothelial dysfunction and oxidative stress during acute transient hyperglycaemia in Type 2 diabetes: a pilot randomized controlled trial. Mellor DD, Madden LA, Smith KA, Kilpatrick ES, Atkin SL. Source Department of Clinical Science, University of Chester, Chester, U.K. Abstract AIMS: To investigate the effects of high-polyphenol chocolate upon endothelial function and oxidative stress in Type 2 diabetes mellitus during acute transient hyperglycaemia induced following a 75-g oral glucose challenge. METHODS: Ten subjects with Type 2 diabetes underwent a double-blinded randomized controlled crossover study. A 75-g oral glucose load was used to induce hyperglycaemia, which was administered to participants 60 min after they had ingested either low (control) or high-polyphenol chocolate. Participants undertook testing at weekly intervals, following an initial cocoa-free period. Endothelial function was assessed by both functional [reactive hyperaemia peripheral artery tonometry (EndoPAT-2000) and serum markers (including intercellular adhesion molecule 1, P-selectin and P-selectin glycoprotein ligand 1]. Urinary 15-F2t-isoprostane adjusted for creatinine was used as an oxidative stress marker. Measurements were made at baseline and 2 h post-ingestion of the glucose load. RESULTS: Prior consumption of high-polyphenol chocolate before a glucose load improved endothelial function (1.7 0.1 vs. 2.3 0.1%, P = 0.01), whereas prior consumption of control chocolate resulted in a significant increase in intercellular adhesion molecule 1 (321.1 7.6 vs. 373.6 10.5 ng/ml, P = 0.04) and 15-F2t-isoprostane (116.8 5.7 vs. 207.1 5.7 mg/mol, P = 0.02). Analysis of percentage changes from baseline comparing control and high-polyphenol chocolate showed a significant improvement for high-polyphenol chocolate in both measures of endothelial function (P < 0.05) and for urinary 15-F2tisoprostane (P = 0.04). CONCLUSION:
Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

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High-polyphenol chocolate protected against acute hyperglycaemia-induced endothelial dysfunction and oxidative stress in individuals with Type 2 diabetes mellitus. 2012 The Authors. Diabetic Medicine 2012 Diabetes UK. 2012 The Authors. Diabetic Medicine 2012 Diabetes UK. PMID: 23039340 [PubMed - as supplied by publisher] Life Sci. 2012 Sep 12. pii: S0024-3205(12)00481-X. doi: 10.1016/j.lfs.2012.08.031. [Epub ahead of print] Ingestion of cocoa ameliorates endothelial dysfunction in mesentery arterioles induced by high fat diet in rats: An in vivo intravital microscopy study. Osakabe N, Shibata M. Source Department of Bio-science and Engineering, Shibaura Institute of Technology, 307 Fukasaku, Munumaku, Saitama, 337-8570, Japan. Electronic address: naoosa@sic.shibaura-it.ac.jp. Abstract Aims:Numerous clinical studies have reported that ingestion of cocoa has a therapeutic effect on hypertension. However, there is only limited information on the mechanism of ingestion of cocoa on arterioles, vessels that have a major role in determining blood pressure. In this study, we used intravital videomicroscopy to evaluate the effect of cocoa consumption on the mesentery microcirculation of rats fed a high fat diet. Main methods:The animals were allocated to 3 groups, and fed either a control diet, a high fat diet containing 15% lard, or the HFD with 2% cocoa (HFD-C) for 6weeks. At the end of the experimental period, the mesentery was spread in a chamber, and the vessels were treated topically with phenylephrine, acetylcholine, or papaverine. The vascular responses to phenylephrine, acetylcholine-dependent vasodilatation and endothelium-independent vasodilatation were calculated by the diameter of the mesentery artery with each treatment. Key findings:Topical treatment of mesenteric arterioles with acetylcholine caused a significantly greater response in the control compared with the HFD group. In the HFD-C group, acetylcholinedependent vasodilatation was decreased marginally. Similarly, rats in the HFD group showed a significant reduction in vascular sensitivity to phenylephrine compared with the control group. However, there was no significant difference between the control and HFD-C groups. The induction of endothelialindependent artery dilation was reduced slightly in the HFD group. Significance:Our results suggest that one of the hypotensive mechanisms of cocoa is due to amelioration of endothelial dysfunction in arterioles induced by an inappropriate diet. Copyright 2012. Published by Elsevier Inc. PMID: 22982348 [PubMed - as supplied by publisher] Oxid Med Cell Longev. 2009 Nov-Dec;2(5):307-16.
Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

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The fat-1 transgene in mice increases antioxidant potential, reduces proinflammatory cytokine levels, and enhances PPAR-gamma and SIRT-1 expression on a calorie restricted diet. Rahman M, Halade GV, Bhattacharya A, Fernandes G. Source Department of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA. rahmanm@uthscsa.edu Abstract Both n-3 fatty acids (FA) and calorie-restriction (CR) are known to exert antiinflammatory and anti-oxidative effects in animals and humans. In this study, we investigated the synergistic anti-inflammatory and anti-oxidative capacity of n-3 FA and CR using Fat-1 transgenic mice (Fat-1) that are capable of converting n6 FA to n-3 FA endogenously. Wild type (WT) and Fat-1 mice were maintained on ad libitum (AL) or CR (40% less than AL) AIN-93 diet supplemented with 10% corn oil (rich in n-6 FA) for 5 months. Significantly lower levels of n-6/n-3 FA ratio were observed in serum, muscle and liver of Fat-1 mice fed AL or CR as compared to that of WT mice fed AL or CR. Muscle catalase (CAT), super oxide dismutase (SOD), glutathione peroxidase (GPX) activities, and liver CAT and SOD activities were found higher in Fat-1 mice as compared to that of WT mice. These activities were more pronounced in Fat-1/CR group as compared to other groups. Serum pro-inflammatory markers, such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-6 were found lower in Fat-1 mice, as compared to that of WT mice. This anti-inflammatory effect was also more pronounced in Fat-1/CR group as compared to that of other groups. Furthermore, significantly higher levels of peroxisome proliferator-activated receptor (PPAR)-gamma and life prolonging gene, sirtuin (SIRT)-1 expression were found in liver of Fat-1/CR mice, as compared to that of WT/CR mice. These data suggest that n-3 FA along with moderate CR may prolong lifespan by attenuating inflammation and oxidative stress. PMID: 20716918 [PubMed - indexed for MEDLINE] Nutr Metab (Lond). 2010 Jul 6;7:55. Effect of long-term treatment with antioxidants (vitamin C, vitamin E, coenzyme Q10 and selenium) on arterial compliance, humoral factors and inflammatory markers in patients with multiple cardiovascular risk factors. Shargorodsky M, Debby O, Matas Z, Zimlichman R. Source Brunner Institute for Cardiovascular Research, Wolfson Medical Center, Holon, 58100, Israel. zimlich@post.tau.ac.il. Abstract BACKGROUND: Antioxidant supplementations have the potential to alleviate the atherosclerotic damage caused by excessive production of reactive oxygen species (ROS). The present study evaluated the effects of prolonged antioxidant treatment on
Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

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arterial elasticity, inflammatory and metabolic measures in patients with multiple cardiovascular risk factors. METHODS: Study participants were randomly assigned to two groups. Group 1 received oral supplementation with 2 capsules per day of Mid Life Guard, SupHerb, Israel. In each capsule vitamin C (500 mg) vitamin E (200 iu), co-enzyme Q10 (60 mg) and selenium (100 mcg), Group 2 received matching placebo(SupHerb) for 6 months. Patients were evaluated for lipid profile, HbA1C, insulin, Cpeptide, hs-CRP, endothelin, aldosterone, plasma renin activity and Homeostasis model assessment-insulin resistance (HOMA-IR). Arterial elasticity was evaluated using pulse wave contour analysis (HDI CR 2000, Eagan, Minnesota). RESULTS: Antioxidant-treated patients exhibited significant increases in large arterial elasticity index (LAEI) as well as small arterial elasticity index (SAEI). A significant decline HbA1C and a significant increase in HDL-cholesterol were also observed. In the placebo group, significant changes in LAEI, SAEI or metabolic measures were not observed. CONCLUSIONS: Antioxidant supplementation significantly increased large and small artery elasticity in patients with multiple cardiovascular risk factors. This beneficial vascular effect was associated with an improvement in glucose and lipid metabolism as well as decrease in blood pressure. PMID: 20604917 [PubMed] Eur J Clin Nutr. 2005 May;59(5):727-30. A thromboxane effect of a hydroxytyrosol-rich olive oil wastewater extract in patients with uncomplicated type I diabetes. Lger CL, Carbonneau MA, Michel F, Mas E, Monnier L, Cristol JP, Descomps B. Source EA Nutrition Humaine et Athrognse, Universit Montpellier 1, Institut de Biologie, France. leger@univ-montp1.fr Abstract OBJECTIVE: To assess the antioxidant/non-antioxidant effects of a hydroxytyrosol (HT)-rich phenolic extract from olive mill wastewaters administered with a breakfast. DESIGN, SETTING AND SUBJECTS: Five type I diabetic patients received 25 mg of HT the first day and 12.5 mg/day the following 3 days. Blood sampling was carried out at T(0) (baseline) and T(4d) just before the breakfast + HT administration and at time points 1, 2, 3 and 4 h after T(0). Urines (24-h) were collected from T(0) to T(4d). Baseline HbA1c was generally inferior to 10%, glycemia was within the range 6-24 mmol/l, whereas total cholesterol, HDL-chol and triglycerides were normal. RESULTS:
Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

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The major finding was the 46% decrease in the serum TXB(2) production after blood clotting at T(4d). Plasma vitamin A, E, beta-carotene were not changed. Vitamin C tended to increase (P = 0.075). Plasma antioxidant capacity was enhanced at T(0)+1 h only, whereas its main determinants (albumin, bilirubin, uric acid) were not modified. Urinary 8-isoPGF(2alpha) levels were highly variable and were not affected significantly by HT administration. CONCLUSION: The major effect of HT accounts for an antiaggregating platelet action, leading to a possible prevention of thrombotic and microthrombotic processes. PMID: 15798774 [PubMed - indexed for MEDLINE] Nutr Metab Cardiovasc Dis. 2009 Dec;19(11):781-8. Epub 2009 Apr 8. Effects of oral magnesium supplementation on insulin sensitivity and blood pressure in normo-magnesemic nondiabetic overweight Korean adults. Lee S, Park HK, Son SP, Lee CW, Kim IJ, Kim HJ. Source Center for Obesity, Nutrition and Metabolism, Department of Family Medicine, Pusan National University Hospital, 1-10 Ami-dong, Seo-gu, Busan 602 739, South Korea. saylee@pnu.edu Abstract BACKGROUND AND AIM: Little is known about the effect of magnesium on insulin sensitivity and BP in healthy individuals. Therefore, we investigated whether magnesium could improve insulin sensitivity and blood pressure (BP) in normo-magnesemic nondiabetic overweight adults. METHODS AND RESULTS: In a double-blinded, placebo-controlled, randomized trial, a total of 155 participants (BMI > or = 23 kg/m(2)) received either 12.3 mmol (300 mg) of elemental magnesium in the form of magnesium oxide (n=75) or placebo (n=80) each day for 12 weeks, constituting the intent-to-treat population. A repeatedmeasures ANOVA was used to evaluate the between-group changes in variables during the study. The baseline characteristics between the intervention and control groups were similar. There were no significant differences between the groups in the pattern of change of the homeostasis model assessment insulin resistance index, BP over time during the 12-week study. In subgroup analysis, magnesium supplementation (n=8, 27, and 24, respectively) lowered BP much more than placebo (n=16, 29, and 25, respectively) in those subjects whose systolic BP > or = 140 mmHg, diastolic BP 80-90 mmHg, and diastolic BP > or = 90 mmHg at the start of the study (P=0.016, 0.043, and 0.023, respectively); in comparison, those subjects whose initial BP reading was low at baseline did not show a change in BP. No significant adverse events related to magnesium supplementation were recorded. CONCLUSIONS:
Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

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These results suggested that magnesium supplementation does not reduce BP and enhance insulin sensitivity in normo-magnesemic nondiabetic overweight people. However, it appears that magnesium supplementation may lower BP in healthy adults with higher BP. PMID: 19359148 [PubMed - indexed for MEDLINE] Hypertension. 2012 Apr;59(4):818-24. Epub 2012 Feb 21. Acute reversal of endothelial dysfunction in the elderly after antioxidant consumption. Wray DW, Nishiyama SK, Harris RA, Zhao J, McDaniel J, Fjeldstad AS, Witman MA, Ives SJ, Barrett-O'Keefe Z, Richardson RS. Source Geriatric Research, Education, and Clinical Center, Salt Lake City Veterans Affairs Medical Center, Salt Lake City, UT 84132, USA. walter.wray@hsc.utah.edu Abstract Aging is associated with a pro-oxidant state and a decline in endothelial function. Whether acute, enteral antioxidant treatment can reverse this decrement in vascular function is not well known. Flow-mediated vasodilation and reactive hyperemia were evaluated after consumption of either placebo or an oral antioxidant cocktail (vitamin C, 1000 mg; vitamin E, 600 IU; -lipoic acid, 600 mg) in 87 healthy volunteers (42 young: 251 years; 45 older: 711 years) using a double-blind, crossover design. Blood velocity and brachial artery diameter (ultrasound Doppler) were assessed before and after 5-minute forearm circulatory arrest. Serum markers of lipid peroxidation, total antioxidant capacity, endogenous antioxidant activity, and vitamin C were assayed, and plasma nitrate, nitrite, and 3-nitrotyrosine were determined. In the placebo trial, an age-related reduction in brachial artery vasodilation was evident (young: 7.40.6%; older: 5.20.4%). After antioxidant consumption, flow-mediated vasodilation improved in older subjects (placebo: 5.20.4%; antioxidant: 8.20.6%) but declined in the young (placebo: 7.40.6%; antioxidant: 5.80.6%). Reactive hyperemia was reduced with age, but antioxidant administration did not alter the response in either group. Together, these data demonstrate that antioxidant consumption acutely restores endothelial function in the elderly while disrupting normal endothelium-dependent vasodilation in the young and suggest that this age-related impairment is attributed, at least in part, to free radicals. Comment in Antioxidants and endothelial dysfunction in young and elderly people: is flowmediated dilation useful to assess acute effects? [Hypertension. 2012] PMID: 22353612 [PubMed - indexed for MEDLINE] Diabetes Care. 2009 May;32(5):810-2. Epub 2009 Feb 19. Coenzyme Q10 improves endothelial dysfunction in statin-treated type 2 diabetic patients.
Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

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Hamilton SJ, Chew GT, Watts GF. Source School of Medicine and Pharmacology, Royal Perth Hospital Unit, University of Western Australia, Perth, Australia. Abstract OBJECTIVE: The vascular benefits of statins might be attenuated by inhibition of coenzyme Q(10) (CoQ(10)) synthesis. We investigated whether oral CoQ(10) supplementation improves endothelial dysfunction in statin-treated type 2 diabetic patients. RESEARCH DESIGN AND METHODS: In a double-blind crossover study, 23 statin-treated type 2 diabetic patients with LDL cholesterol <2.5 mmol/l and endothelial dysfunction (brachial artery flowmediated dilatation [FMD] <5.5%) were randomized to oral CoQ(10) (200 mg/day) or placebo for 12 weeks. We measured brachial artery FMD and nitrate-mediated dilatation (NMD) by ultrasonography. Plasma F(2)-isoprostane and 24-h urinary 20-hydroxyeicosatetraenoic acid (HETE) levels were measured as systemic oxidative stress markers. RESULTS: Compared with placebo, CoQ(10) supplementation increased brachial artery FMD by 1.0 +/- 0.5% (P = 0.04), but did not alter NMD (P = 0.66). CoQ(10) supplementation also did not alter plasma F(2)-isoprostane (P = 0.58) or urinary 20-HETE levels (P = 0.28). CONCLUSIONS: CoQ(10) supplementation improved endothelial dysfunction in statin-treated type 2 diabetic patients, possibly by altering local vascular oxidative stress. PMID: 19228872 [PubMed - indexed for MEDLINE]

Direitos Autorais Protegidos pela Lei 9.610 de 19 de Fevereiro de 1998. Estas informaes devem ser analisadas pelo profissional prescrito antes de adotadas na prtica, e so de distribuio e uso exclusivo de mdicos, farmacuticos, dentistas e outros profissionais autorizados a prescrever. proibida a veiculao para pblico leigo, por meios eletrnicos, ou de qualquer outro modo que no seja diretamente para profissionais autorizados a prescrever. proibida a alterao parcial ou total deste material. A Biopharma no autoriza e no se responsabiliza por qualquer alterao efetuada neste material. www.biopharma.com.br. 34 3233.1200.

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