Treatment Weight Before (g) Weight After (g) Length After (mm)

A1 1.36 0.93 33.96

A2 1.75 0.94 33.8
A3 0.88 0.6 34.02
A4 1.8 1.2 35.29
A5 1.49 1.16 35.97
A6 1.76 0.95 34.62
B1 1.48 0.47 29.57
B2 1.36 1.13 37.5
B3 1.48 1.29 37.31
B4 1.52 0.6 33.22
B5 0.6 0.57 32.91
B6 0.9 0.52 30.17
C1 1.62 0.88 33.28
C2 1.59 1.26 36.29
C3 2.05 1.1 34.48
C4 1.22 0.75 34.32
C5 1.28 0.97 33.5
C6 0.67 0.52 31.4
D1 1.01 0.82 31.9
D2 1.43 0.82 35.4
D3 1.34 0.12 29.43
D4 1.53 1.19 35.79
D5 1.79 0.57 32.25
D6 0.97 0.44 30.61
Treatment Temp- Day Day 8 Day 9 Day 12 Treatment pH- day 4 Day 8 Day 9 Day 12
A1 24 23 29 26 A1 7.15 6.5 6.58 6.68
A2 24 23.5 29 25.5 A2 7.19 6.55 6.71 6.6
A3 24 24 29 25 A3 7.23 6.58 6.31 6.32
A4 25 24 29.5 26 A4 7.21 6.49 6.5 6.24
A5 24.5 24 28 24 A5 7.63 6.53 6.98 6.28
A6 24.5 23 29 26 A6 7.67 6.58 6.33 6.24
B1 24 23 29 25.5 B1 7.22 6.41 6.38 6.56
B2 24.5 23.5 29 26 B2 7.35 6.3 6.58 6.51
B3 24.5 23.5 29 25 B3 7.25 6.32 5.93 6.36
B4 24 23 28.5 25.5 B4 7.4 6.31 6.31 6.55
B5 24 23.5 29 25 B5 7.31 6.12 6.3 5.82
B6 24 23 28.5 26 B6 7.51 6.22 6.11 6.1
C1 24 23 29.5 25.5 C1 7.79 6.29 6.22 6.63
C2 24 23 29 26 C2 7.48 6.3 6.58 6.45
C3 24 23.5 29.5 25 C3 7.59 6.49 6.12 6.46
C4 24 22.5 29 25 C4 7.6 6.45 6.34 6.22
C5 24.5 23.5 28.5 26.5 C5 7.73 6.52 5.94 6.15
C6 24.5 23 29 26 C6 7.65 6.16 6.1 6.68
D1 24 22.5 29.5 25 D1 7.6 6.17 6.52 6.65
D2 23.5 22 29 25 D2 7.9 6.25 5.92 6.45
D3 24 0 0 0 D3 7.8 0 0 0
D4 24 22.5 28.5 25 D4 7.75 6.73 5.95 6.39
D5 25 23 29 26 D5 8 6.33 5.98 6.8
D6 25 22 28.5 26 D6 8.08 6.6 6.15 6.57
Treatment Respi Rate- Day 4 Day 8 Day 9
T1 T2 T3 Ave T1 T2 T3 Ave T1
A1 164 176 164 168 80 92 92 88
A2 120 136 136 130.67 100 92 100 97.33
A3 144 128 132 134.67 128 96 128 117.33
A4 116 108 96 106.67 92 76 96 88
A5 152 172 168 164 120 76 88 94.67
A6 116 80 120 105.33 84 88 104 92
B1 88 112 96 98.67 96 124 136 118.67
B2 120 92 104 105.33 80 76 112 89.33
B3 220 208 220 218.67 96 92 124 104
B4 56 56 44 52 120 84 84 96
B5 92 92 128 104 100 76 104 93.33
B6 176 184 144 168 104 112 128 114.67
C1 140 84 80 101.33 112 128 116 118.67
C2 136 80 92 102.67 112 132 140 128
C3 148 172 132 150.67 100 116 112 109.33
C4 84 64 92 80 112 112 120 114.67
C5 128 116 172 138.67 140 116 116 124
C6 80 96 56 77.33 136 144 132 137.33
D1 80 80 72 77.33 44 60 56 53.33
D2 120 120 88 111.33 72 92 72 78.67
D3 56 76 52 61.33
D4 128 136 164 142.67 68 56 44 56
D5 80 80 84 81.33 64 68 72 68
D6 56 52 44 50.67 64 76 68 69.33

Treatment Day 4 AverDay 8 AverDay 9 AverDay 12 Average

A1 168 88 270 25.33
A2 130.67 97.33 180 52
A3 134.67 117.33 189 22.67
A4 106.67 88 213 36
A5 164 94.67 183 33.33
A6 105.33 92 189 22.67
B1 98.67 118.67 69 22.67
B2 105.33 89.33 72 34.67
B3 218.67 104 78 41.33
B4 52 96 69 20
B5 104 93.33 78 66.67
B6 168 114.67 81 14.67
C1 101.33 118.67 87 26.67
C2 102.67 128 78 34.67
C3 150.67 109.33 72 33.67
C4 80 114.67 72 45.33
C5 138.67 124 87 56
C6 77.33 137.33 78 22.67
D1 77.33 53.33 144 22.67
D2 111.33 78.67 153 21.33
D3 61.33 0 0 0
D4 142.67 56 183 45.33
D5 81.33 68 153 36
D6 50.67 69.33 159 17.33
 Day 9 Day 12
T2 T3 Ave T1 T2 T3 Ave
270 40 20 16 25.33
180 48 52 56 52
189 24 24 20 22.67
213 32 40 36 36
183 44 28 28 33.33
189 20 28 20 22.67
69 32 16 20 22.67
72 28 40 36 34.67
78 28 44 52 41.33
69 20 24 16 20
78 84 48 68 66.67
81 12 12 20 14.67
87 20 36 24 26.67
78 44 36 24 34.67
72 40 40 32 33.67
72 44 56 36 45.33
87 76 56 36 56
78 24 24 20 22.67
144 20 32 16 22.67
153 20 20 24 21.33
0
183 32 52 52 45.33
153 40 24 44 36
159 20 16 16 17.33
Treatment Amt of NaOH- Day 4 Day 8 Day 9 Day 12 Died Treatment
A1 10 1.6 1.5 1 A1
A2 6 1.5 1.8 0.85 A2
A3 4.5 1.6 1.5 1.1 A3
A4 4 1.5 1.6 1 A4
A5 4.25 1.4 1.5 0.9 A5
A6 3 1.6 1.5 1.4 A6
B1 3 1.7 1.5 0.7 B1
B2 2.75 1.85 1.7 1.9 B2
B3 2.9 2.3 3 1.1 B3
B4 2.9 1 1.5 0.8 B4
B5 3 3 1.5 1.5 B5
B6 2.8 1.5 1.6 1.5 B6
C1 2.75 2 1.8 0.8 C1
C2 2.7 2.3 1.6 1 C2
C3 3.6 2.7 1.85 1.3 C3
C4 2.9 1.8 1.8 1.3 C4
C5 2.5 2 1.6 1 C5
C6 2.9 1.5 1.5 1 C6
D1 2.9 1.2 1.5 1.1 D1
D2 3.2 1.7 1.7 1.15 D2
D3 3.2 0 0 0 Apr 16- 1.5 D3
D4 4 2.9 1.6 1.1 D4
D5 3.7 1.5 1.4 0.9 D5
D6 3.4 1.5 1.3 1.55 D6
moles CO2 Day 4 Day 8 Day 9 Day 12 Treatment mL CO2 Day 4 Day 8 Day 9
0.002 0.00032 0.0003 0.0002 A1 48.49495 7.759192 7.274242
0.0012 0.0003 0.00036 0.00017 A2 29.09697 7.274242 8.729091
0.0009 0.00032 0.0003 0.00022 A3 21.82273 7.759192 7.274242
0.0008 0.0003 0.00032 0.0002 A4 19.39798 7.274242 7.759192
0.00085 0.00028 0.0003 0.00018 A5 20.61035 6.789293 7.274242
0.0006 0.00032 0.0003 0.00028 A6 14.54848 7.759192 7.274242
0.0006 0.00034 0.0003 0.00014 B1 14.54848 8.244141 7.274242
0.00055 0.00037 0.00034 0.00038 B2 13.33611 8.971566 8.244141
0.00058 0.00046 0.0006 0.00022 B3 14.06354 11.15384 14.54848
0.00058 0.0002 0.0003 0.00016 B4 14.06354 4.849495 7.274242
0.0006 0.0006 0.0003 0.0003 B5 14.54848 14.54848 7.274242
0.00056 0.0003 0.00032 0.0003 B6 13.57859 7.274242 7.759192
0.00055 0.0004 0.00036 0.00016 C1 13.33611 9.69899 8.729091
0.00054 0.00046 0.00032 0.0002 C2 13.09364 11.15384 7.759192
0.00072 0.00054 0.00037 0.00026 C3 17.45818 13.09364 8.971566
0.00058 0.00036 0.00036 0.00026 C4 14.06354 8.729091 8.729091
0.0005 0.0004 0.00032 0.0002 C5 12.12374 9.69899 7.759192
0.00058 0.0003 0.0003 0.0002 C6 14.06354 7.274242 7.274242
0.00058 0.00024 0.0003 0.00022 D1 14.06354 5.819394 7.274242
0.00064 0.00034 0.00034 0.00023 D2 15.51838 8.244141 8.244141
0.00064 0 0 0 D3 15.51838 0 0
0.0008 0.00058 0.00032 0.00022 D4 19.39798 14.06354 7.759192
0.00074 0.0003 0.00028 0.00018 D5 17.94313 7.274242 6.789293
0.00068 0.0003 0.00026 0.00031 D6 16.48828 7.274242 6.304343
Day 12
4.849495
4.122071
5.334444
4.849495
4.364545
6.789293
3.394646
9.21404
5.334444
3.879596
7.274242
7.274242
3.879596
4.849495
6.304343
6.304343
4.849495
4.849495
5.334444
5.576919
0
5.334444
4.364545
7.516717
Treatment Behavior Day 4
0 1 2 3 4 5 6 7 8
A1 2 3 3 1 3 2 2 1 12
A2 3 3 3 3 3 2 3 3 2
A3 3 3 3 3 3 3 3 3 2
A4 3 3 2 3 3 2 2 2 3
A5 2 1 1 1 1 3 2 3 3
A6 2 3 1 2 2 3 3 3 1
B1 3 2 2 2 2 4 3 3 3
B2 3 2 2 2 2 2 2 2 1
B3 1 2 2 2 2 2 2 2 2
B4 2 1 2 1 1 1 1 1 1
B5 3 2 3 2 3 1 1 2 1
B6 2 3 3 3 2 2 2 2 2
C1 3 3 3 3 2 3 3 3 3
C2 3 1 1 1 1 1 2 2 3
C3 3 1 1 1 2 1 1 1 1
C4 3 3 1 1 1 1 2 2 1
C5 1 1 1 1 1 1 1 1 1
C6 3 4 1 3 2 1 2 2 1
D1 2 2 1 1 2 2 2 2 1
D2 3 1 2 3 2 3 3 3 1
D3 3 3 2 2 2 2 3 1 1
D4 1 1 1 1 1 1 3 1 1
D5 1 1 1 1 1 1 3 1 1
D6 2 2 1 3 1 2 3 2 1
or Day 4
9 10 11 12 13 14 15
2 1 1 1 1 1 1
3 3 3 2 3 3 3
3 4 3 2 2 3 3
2 3 2 2 2 3 3
3 1 1 1 1 2 1
2 1 1 1 1 2 1
3 2 2 3 3 3 3
2 2 2 2 2 2 2
2 2 2 2 2 1 2
2 1 1 1 1 1 1
2 2 1 1 3 2 2
1 2 2 1 2 2 2
3 2 2 2 2 2 2
1 1 1 1 1 1 1
1 1 1 1 1 1 1
1 1 2 1 1 1 1
1 1 1 1 1 1 2
2 1 1 1 1 2 1
1 1 1 1 2 1 2
2 2 2 2 1 1 1
2 2 2 4 1 1 2
2 1 2 1 1 1 1
1 1 1 1 1 1 1
2 2 2 1 2 3 3
Treatment Day 4 AverDay 8 AverDay 9 AverDay 12 Average
A1 2.3125 2.125 2.3125 1.9375
A2 2.8125 2 2 1.8125
A3 2.875 2.4375 2.875 2.6875
A4 2.5 2 2 1.875
A5 1.6875 2.1875 2.1875 2.1875
A6 1.8125 2.3125 2.6875 2.5
B1 2.6875 3.6875 2.3125 2.5
B2 2 2.875 2 2.0625
B3 1.875 1.9375 2.1875 1.5
B4 1.1875 1.0625 1.75 1.5
B5 1.9375 2.375 2.8125 2.0625
B6 2.0625 2.6875 2.75 2.3125
C1 2.5625 2.1875 2.125 2.3125
C2 1.375 2.1875 2.0625 2.1875
C3 1.1875 2.3125 2.25 1.125
C4 1.4375 2.5 2.5625 1.8125
C5 1.0625 1.9375 2.3125 1.9375
C6 1.75 2.1875 2.625 2.3125
D1 1.5 2.0625 2.125 2.1875
D2 2 3 2.25 2.375
D3 2.0625 0 0 0
D4 1.25 1.1875 2.25 1.875
D5 1.125 1.625 2.625 1.1875
D6 2 1.5 2.0625 1.875
treatment Behavior- Day 8
0 1 2 3 4 5 6 7 8
A1 2 2 1 1 3 2 2 2 3
A2 2 2 2 2 2 2 2 2 2
A3 3 2 2 2 2 3 3 2 3
A4 2 2 2 2 2 2 2 2 2
A5 2 2 2 2 2 3 2 2 2
A6 2 2 2 2 2 2 2 2 2
B1 4 3 3 2 3 4 4 4 4
B2 3 3 2 3 2 3 3 3 3
B3 3 3 2 1 3 2 2 3 1
B4 1 1 1 1 1 1 1 1 1
B5 2 3 2 2 2 2 2 2 2
B6 2 3 2 2 4 3 3 3 3
C1 2 2 3 2 1 2 2 2 2
C2 2 2 3 3 1 2 2 2 2
C3 2 3 2 2 2 2 2 3 3
C4 2 3 2 2 2 3 4 4 2
C5 2 3 2 1 2 2 1 2 1
C6 2 2 2 2 2 2 1 2 1
D1 2 2 3 2 2 2 2 2 2
D2 3 2 3 2 3 3 3 4 4
D3 0 0 0 0 0 0 0 0 0
D4 2 1 1 1 1 2 1 2 1
D5 2 3 2 3 2 3 2 1 1
D6 3 1 1 1 1 1 1 1 1
or- Day 8
9 10 11 12 13 14 15
3 2 2 3 2 2 2
2 2 2 2 2 2 2
2 3 3 3 2 2 2
2 2 2 2 1 3 2
2 2 2 2 2 4 2
3 3 3 3 3 1 3
4 4 4 4 4 4 4
3 3 3 3 4 2 3
1 2 2 2 1 1 2
1 1 1 2 1 1 1
2 3 3 3 2 4 2
3 3 3 2 2 3 2
3 2 2 3 2 2 3
3 2 1 2 2 2 4
4 2 2 2 2 2 2
3 3 2 2 2 2 2
1 2 3 3 2 2 2
2 4 4 4 1 3 1
2 2 2 2 2 2 2
4 3 3 2 4 3 2
0 0 0 0 0 0 0
1 1 1 1 1 1 1
1 1 1 1 1 1 1
1 2 1 1 3 2 3
Treatement Behavio
0 1 2 3 4 5 6
A1 3 2 2 3 2 3 2
A2 2 2 2 2 2 2 2
A3 3 3 3 3 3 2 2
A4 2 2 2 2 2 2 2
A5 3 2 2 1 2 2 2
A6 3 3 1 3 3 3 3
B1 3 2 3 3 2 3 2
B2 2 2 2 2 2 2 2
B3 2 2 2 2 3 2 2
B4 2 2 3 1 2 1 2
B5 3 3 3 3 3 3 2
B6 2 2 3 3 3 3 3
C1 2 2 2 2 3 2 2
C2 2 2 2 2 3 2 2
C3 3 2 2 2 3 2 2
C4 2 3 3 3 3 3 2
C5 2 1 3 2 2 2 2
C6 2 3 2 3 2 3 3
D1 2 2 2 2 2 2 2
D2 3 3 2 2 3 2 2
D3 0 0 0 0 0 0 0
D4 2 3 2 2 3 3 2
D5 3 3 2 3 3 3 2
D6 3 2 2 2 2 2 2
Behavior- Day 9
7 8 9 10 11 12 13 14
3 2 3 2 2 2 2 2
2 2 2 2 2 2 2 2
3 3 3 3 3 3 3 3
2 2 2 2 2 2 2 2
3 2 3 2 2 2 2 2
2 3 3 2 2 3 3 3
2 2 2 3 2 2 2 2
2 2 2 2 2 2 2 2
3 2 2 2 2 2 2 3
1 1 2 2 2 1 2 2
3 3 3 2 2 3 3 3
3 3 3 2 2 3 3 3
2 2 3 2 2 2 2 2
2 2 2 2 2 2 2 2
3 2 2 2 2 2 3 2
3 3 2 3 2 2 2 3
3 2 3 2 3 2 3 2
3 3 2 3 3 3 2 2
2 2 3 2 2 2 3 2
3 2 2 2 2 2 2 2
0 0 0 0 0 0 0 0
2 3 2 2 2 2 2 2
2 3 3 3 2 2 3 2
2 2 2 2 2 2 2 2
15
2
2
3
2
3
3
2
2
2
2
3
3
2
2
2
2
3
3
2
2
0
2
3
2
Treatment Day
0 1 2 3 4 5 6
A1 2 2 2 3 2 3 2
A2 2 3 2 2 2 1 2
A3 2 2 3 3 3 2 3
A4 1 2 2 1 1 3 3
A5 4 1 2 2 3 2 3
A6 2 3 2 3 3 2 2
B1 2 3 2 3 3 2 2
B2 2 2 3 2 2 2 2
B3 2 2 2 2 1 2 2
B4 3 1 2 1 1 1 1
B5 3 2 3 2 3 2 2
B6 3 2 3 2 3 1 3
C1 2 3 3 3 2 2 2
C2 2 2 3 2 2 2 3
C3 1 3 1 1 1 1 1
C4 3 2 1 1 1 1 3
C5 2 3 3 3 2 2 1
C6 2 2 2 2 3 1 2
D1 2 3 2 2 3 2 2
D2 2 3 2 3 3 3 2
D3 0 0 0 0 0 0 0
D4 3 3 2 2 3 1 1
D5 1 1 1 3 1 1 1
D6 2 2 2 2 2 2 2
Day 12
7 8 9 10 11 12 13 14
3 2 2 2 2 1 1 1
2 2 1 2 2 2 2 1
2 2 3 3 3 3 3 3
2 2 2 2 1 1 2 3
2 1 2 3 2 2 2 2
3 3 3 2 2 2 3 3
3 3 3 2 2 2 3 3
2 2 2 2 2 2 2 2
2 1 1 1 1 1 2 1
1 2 1 1 3 1 1 2
2 1 2 1 2 2 2 3
1 3 1 2 2 3 2 3
2 3 2 2 2 2 2 2
2 2 3 2 2 2 2 2
1 1 1 1 1 1 1 1
2 2 1 2 1 3 2 2
2 2 1 2 2 2 1 1
2 3 3 2 2 3 2 3
2 3 2 2 2 2 2 2
2 2 2 2 2 3 2 3
0 0 0 0 0 0 0 0
1 1 2 2 2 1 2 2
1 1 1 1 2 1 1 1
2 1 2 2 2 1 2 2
15
1
1
3
2
2
2
2
2
1
2
1
3
3
2
1
2
2
3
2
2
0
2
1
2