concentração inicial de sacarose 30s 1m 1m 2m 5m

10 180.2 360.3
20 270.3
40 360.3
60 405.4
80 432.4
100 450.4

Velocidade inicial
1000
900
800
700
600
500
400
300
200
100
0
0 20 40 60 80
 10m 15m velocidade inicial
360.4
540.6
720.6
810.8
864.8
900.8

elocidade inicial

60 80 100 120
 concentração de s velocidade inicial 1/S 1/V a)
1.00E-02 7.50E-05 1.00E+02 1.33E+04 Vmax=
1.00E-03 7.50E-05 1.00E+03 1.33E+04 Km=
1.00E-04 6.00E-05 1.00E+04 1.67E+04
7.50E-05 5.63E-05 1.33E+04 1.78E+04 b)
6.25E-06 1.50E-05 1.60E+05 6.67E+04

7.00E+04
c)
f(x) = 0.334024709x + 13234.4974573139
6.00E+04 R² = 0.9999641913 1/S
1/S
5.00E+04

4.00E+04 8.29E+03

3.00E+04
1.21E-04
2.00E+04

1.00E+04

0.00E+00
0.00E+002.00E+044.00E+046.00E+048.00E+041.00E+051.20E+051.40E+051.60E+051.80E+05
7.55629439E-05
2.52984736E-05

2.50E-05
2.66E+04
3.76E-05

1.00E-04
1.51E-04

1.66E+04

6.03E-05
 0,5Vmax
1/Vo=Km/Vmax*1/s+1/Vmax

1/0,5Vmax=Km/Vmax*1/s+1/Vmax

1/0,5=Km/s+1 1/0,9=Km/s+1 1/0,95=Km/s+1

2 1.1111111111 1.0526315789
1 0.1111111111 0.0526315789
s=1Km s=9Km s=19Km
1/1=Km/s+1

1
0
s=0Km
numero de mols do oxigenio
1.51785714285714E-05
numero de mols de peroxido
3.03571428571429E-05
numero de mols da enzima
1.11444444444444E-11
concenntraçao da enzima
1.11444444444444E-09
 Kcat
2.72E+06
S v 1/s 1/v
8.35E-06 1.38E-05 1.20E+05 7.25E+04
1.00E-05 1.60E-05 1.00E+05 6.25E+04
lineweave
1.25E-05 1.91E-05 8.00E+04 5.24E+04
8.00E+04
1.67E-05 2.38E-05 5.99E+04 4.20E+04
2.00E-05 2.67E-05 5.00E+04 3.75E+04 7.00E+04
2.50E-05 3.08E-05 4.00E+04 3.25E+04 6.00E+04
3.30E-05 3.62E-05 3.03E+04 2.76E+04
5.00E+04
5.00E-05 4.45E-05 2.00E+04 2.25E+04
1.00E-04 5.72E-05 1.00E+04 1.75E+04 4.00E+04
2.00E-04 6.67E-05 5.00E+03 1.50E+04 3.00E+04

2.00E+04

1.00E+04

0.00E+00
0.00E+00 2.00E+04 4.00E+04 6.00E+04 8.
 lineweaver

4.00E+04 6.00E+04 8.00E+04 1.00E+05 1.20E+05 1.40E+05

I. Competitivo
II. Não competitivo
III. Competitivo
 a) b)
Kcat=Vmax/Et
Kcat= 30sE-1
1/Vo=Km/Vmax*1/s+1/Vmax 1/Vo=Km/Vmax*1/s+1/Vmax
km= 5.00E-03 S=1/2km
4=Km/s+1 1/Vo=Km/Vmax*1/0,5Km+1/Vmax
1/Vo=Vmax*1/0,5+1/Vmax
3 1/Vo=2/Vmax+1/Vmax
s= 1.67E-03
Et= 0.05 Vmax=3Vo

c)
Ks = [E][S]/[ES] = k^-1/ k1 = 1/Ka
quanto menor Km, maior a
afinidade
1/Vo=Km/Vmax*1/s+1/Vmax 1/Vo=Km/Vmax*1/s+1/Vmax
S=2km S=10km
1/Vo=Km/Vmax*1/2Km+1/Vmax 1/Vo=Km/Vmax*1/10Km+1/Vmax
1/Vo=0,5/Vmax+1/Vmax 1/Vo=0,1/Vmax+1/Vmax

Vmax=1,5Vo Vmax=1,1Vo
 1/S 1/V
2.50E-06 2.80E-05 4.00E+05 35714.29 1.41E-04 Vmax=
4.00E-06 4.00E-05 2.50E+05 25000 1.01E-05 Km=
1.00E-05 7.00E-05 1.00E+05 14285.71
2.00E-05 9.50E-05 5.00E+04 10526.32
4.00E-05 1.12E-04 2.50E+04 8928.571 Ch
1.00E-04 1.28E-04 1.00E+04 7812.5 40000
2.00E-03 1.39E-04 5.00E+02 7194.245
35000
1.00E-02 1.40E-04 1.00E+02 7142.857 f(x) = 0.0714983412x + 7111.4019
R² = 0.9999613608
1.00E-01 1.40E-04 1.00E+01 7142.857 30000

25000

20000

15000

10000

5000

0
0.00E+00 1.00E+05 2.00E+0
 Chart Title

983412x + 7111.4019146528
13608

0E+05 2.00E+05 3.00E+05 4.00E+05 5.00E+05