Bioinformtica - Milene Rato

Apresentao dos dados

Os dados utilizados referem-se a um projeto sobre a poluio da costa portuguesa por metais e a influncia
da mesma sobre a condio designada por imposexo usando o molusco Nassarius reticulatus como
bioindicador. O imposexo caracteriza-se pelo desenvolvimento de caratersticas masculinas em fmeas
(incluindo o crescimento de um pnis) e avaliado de acordo com o vas deferens sequence index (VDSI).

Anlise descritiva

Das oito variveis apresentadas, setes so escalares (Cu_sed, TBT_sed, Cu_female, Cu_male, VDS, H_shell,
L_penis) e uma ordinal (Distance: 0 junto a fontes de poluio, < 5km; 1 prximo a fontes de poluio,
> 5km e < 10km, 2 afastado de fontes de poluio).

Numa primeira anlise s variveis estudadas, verificou-se a algumas no seguiam uma distribuio normal.
Para a maioria das variveis foi possvel obter uma distribuio normal removendo os outliers.

1. Cu_sed
Refere-se concentrao de cobre em sedimentos.

Com outliers

Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

Cu_sed ,244 45 ,000 ,665 45 ,000

a. Lilliefors Significance Correction

Bioinformtica - Milene Rato
Sem outliers

Case Processing Summary

Cases

Valid Missing Total

N Percent N Percent N Percent

Cu_sed 38 100,0% 0 ,0% 38 100,0%

Descriptives
Statistic Std. Error

Cu_sed Mean 19,3397 2,28363

95% Confidence Interval for Lower Bound 14,7127

Mean Upper Bound 23,9668

5% Trimmed Mean 18,8598

Median 18,5100

Variance 198,169

Std. Deviation 14,07724

Minimum 1,50

Maximum 45,87

Range 44,37

Interquartile Range 28,06

Skewness ,254 ,383

Kurtosis -1,230 ,750

Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

Cu_sed ,125 38 ,139 ,922 38 ,011

a. Lilliefors Significance Correction

Bioinformtica - Milene Rato

2. TBT_sed
Refere-se concentrao de TBT (tributilestanho) em sedimentos.

Com outliers

Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.

TBT_sed ,273 45 ,000 ,687 45 ,000

a. Lilliefors Significance Correction

Bioinformtica - Milene Rato

Sem outliers

Case Processing Summary

Cases

Valid Missing Total

N Percent N Percent N Percent

TBT_sed 36 100,0% 0 ,0% 36 100,0%

Descriptives

Statistic Std. Error

TBT_sed Mean ,0497 ,00781

95% Confidence Interval for Lower Bound ,0339

Mean Upper Bound ,0656

5% Trimmed Mean ,0466

Median ,0300

Variance ,002

Std. Deviation ,04687

Minimum ,00

Maximum ,16

Range ,16
Interquartile Range ,08

Skewness ,977 ,393

Kurtosis -,369 ,768

Bioinformtica - Milene Rato

Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

TBT_sed ,219 36 ,000 ,831 36 ,000

a. Lilliefors Significance Correction

Bioinformtica - Milene Rato
3. Cu_females
Refere-se concentrao de cobre em tecidos de N. reticulatus fmea.
Com outliers

Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

Cu_female ,137 45 ,034 ,858 45 ,000

a. Lilliefors Significance Correction

Sem outliers

Case Processing Summary

Cases

Valid Missing Total

N Percent N Percent N Percent

Cu_female 39 100,0% 0 ,0% 39 100,0%

Bioinformtica - Milene Rato
Descriptives

Statistic Std. Error

Cu_female Mean 217,5056 13,58786

95% Confidence Interval for Lower Bound 189,9985

Mean Upper Bound 245,0128

5% Trimmed Mean 215,6995

Median 216,1000

Variance 7200,569

Std. Deviation 84,85617

Minimum 83,18

Maximum 387,28

Range 304,10

Interquartile Range 146,63

Skewness ,256 ,378

Kurtosis -,902 ,741

Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

Cu_female ,096 39 ,200* ,964 39 ,246

a. Lilliefors Significance Correction

*. This is a lower bound of the true significance.
Bioinformtica - Milene Rato

4. Cu_male
Refere-se concentrao de cobre em tecidos de N. reticulatus fmea.

Com outliers

Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

Cu_male ,212 45 ,000 ,816 45 ,000

a. Lilliefors Significance Correction

Bioinformtica - Milene Rato

Sem outliers

Case Processing Summary

Cases

Valid Missing Total

N Percent N Percent N Percent

Cu_male 37 100,0% 0 ,0% 37 100,0%

Descriptives

Statistic Std. Error

Cu_male Mean 202,1138 13,66857

95% Confidence Interval for Lower Bound 174,3926

Mean Upper Bound 229,8349

5% Trimmed Mean 199,5486

Median 199,1600

Variance 6912,705

Std. Deviation 83,14268

Minimum 60,15

Maximum 387,21

Range 327,06

Interquartile Range 126,44

Skewness ,448 ,388

Kurtosis -,415 ,759
Bioinformtica - Milene Rato

Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

Cu_male ,088 37 ,200* ,961 37 ,218

a. Lilliefors Significance Correction

*. This is a lower bound of the true significance.
Bioinformtica - Milene Rato

5. VDS
Refere-se avaliao do desenvolvimento das caractersticas masculinas em fmeas.

Case Processing Summary

Cases

Valid Missing Total

N Percent N Percent N Percent

VDS 45 100,0% 0 ,0% 45 100,0%

Descriptives

Statistic Std. Error

VDS Mean 2,0360 ,19622

95% Confidence Interval for Lower Bound 1,6405

Mean Upper Bound 2,4315

5% Trimmed Mean 2,0070

Median 1,9600

Variance 1,733

Std. Deviation 1,31628

Minimum ,00

Maximum 4,62

Range 4,62

Interquartile Range 2,04

Skewness ,397 ,354

Kurtosis -,865 ,695

Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

VDS ,127 45 ,065 ,951 45 ,054

a. Lilliefors Significance Correction

Bioinformtica - Milene Rato
Bioinformtica - Milene Rato

6. Distance
Refere-se distncia entre o local de amostragem e as fontes de poluio.

Statistics
Distance

N Valid 45

Missing 0

Distance

Cumulative
Frequency Percent Valid Percent Percent

Valid junto a locais de poluio 18 40,0 40,0 40,0

(<5km)

prximo a fontes de 19 42,2 42,2 82,2

poluio (>5km e <10km)
afastado de fontes de 8 17,8 17,8 100,0
poluio (>10km)

Total 45 100,0 100,0

7. H_shell_male
Refere-se altura da concha de N.reticulatus macho

Com outliers
Bioinformtica - Milene Rato
Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

H_shell_male ,219 37 ,000 ,886 37 ,001

a. Lilliefors Significance Correction

Sem outliers

Case Processing Summary

Cases

Valid Missing Total

N Percent N Percent N Percent

H_shell_male 40 100,0% 0 ,0% 40 100,0%

Bioinformtica - Milene Rato
Descriptives

Statistic Std. Error

H_shell_male Mean 12,9283 ,71524

95% Confidence Interval for Lower Bound 11,4815

Mean Upper Bound 14,3750

5% Trimmed Mean 12,7744

Median 12,6950

Variance 20,462

Std. Deviation 4,52355

Minimum 5,06

Maximum 22,97

Range 17,91

Interquartile Range 6,87

Skewness ,478 ,374

Kurtosis -,196 ,733

Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

H_shell_male ,079 40 ,200* ,964 40 ,221

a. Lilliefors Significance Correction

*. This is a lower bound of the true significance.
Bioinformtica - Milene Rato

8. L_penis_male
Refere-se ao comprimento mdio do pnis em N. reticulatus macho.

Com outliers

Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

L_penis_male ,139 45 ,029 ,883 45 ,000

a. Lilliefors Significance Correction

Bioinformtica - Milene Rato

Sem outliers

Case Processing Summary

Cases

Valid Missing Total

N Percent N Percent N Percent

L_penis_male 40 100,0% 0 ,0% 40 100,0%

Descriptives

Statistic Std. Error

L_penis_male Mean 3,6750 ,32483

95% Confidence Interval for Lower Bound 3,0180

Mean Upper Bound 4,3320

5% Trimmed Mean 3,6067

Median 3,6000

Variance 4,221

Std. Deviation 2,05440

Minimum ,32

Maximum 9,00

Range 8,68

Interquartile Range 3,64

Skewness ,383 ,374

Kurtosis -,375 ,733

Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

L_penis_male ,099 40 ,200* ,969 40 ,347

a. Lilliefors Significance Correction

*. This is a lower bound of the true significance.
Bioinformtica - Milene Rato
Bioinformtica - Milene Rato
Anlise de dados

Cu_sed vs TBT_sed