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1 INTRODUÇÃO
2 MATERIAL E MÉTODOS
As leveduras utilizadas foram cedidas pelo Prof. Dr. Allen Norton Hagler,
do Instituto de Microbiologia da Universidade Federal do Rio de Janeiro, ao
passo que as bactérias pertenciam à coleção de culturas do Laboratório de
Microbiologia de Alimentos - DETA/UNESP, São José do Rio Preto (SP),
Brasil.
4 CONCLUSÃO
Abstract
The in vitro antimicrobial activity was determined for essential oils of cinnamon, clove,
ginger, and mint using three different concentrations (10.0, 1.0 and 0.1%) on twenty-one
microorganisms (seven species of yeasts, and 14 species of bacteria). The study involved
standardized inoculum of microorganisms – previously grown in nutrient broth and sown
through the “in replica plating technique” in Petri dishes containing nutrient agar plus tween
20 – with no treatment (control) and with addition of the different concentrations of
essential oils. The results obtained after incubation at 30° C for thirty days showed that,
within the parameters used in the experiment, the essential oils of cinnamon and mint
presented the highest antimicrobial activity in the concentrations of 10.0 and 1.0%,
followed by that from clove in the concentration of 10.0%, which also completely inhibited
the growth of all the tested microorganisms.
Leveduras
Candida tropicalis + - - - - - + + + + - - +
Cryptococcus laurentii + - - - - - + + + + - - +
Hansenula anomala + - - - - - + + + + - - +
Kluyveromyces marxianus + - - - - + + + + + - - +
Pichia burtonii + - - - - - + + + + - - +
Rhodotorula rubra + - - - - - + + + + - - +
Sporobolomyces roseus + - - + - + + + + + - - +
Bactérias
Azotobacter sp + - - - - + + + + + - - +
Bacillus brevis + - - + - + + + + + - - +
Bacillus cereus + - - + - + + + + + - - +
Bacillus circulans + - - + - + + + + + - - +
Bacillus polymyxa + - - + - + + + + + - - +
Bacillus stearothermophilus + - - + - + + + + + - - +
Bacillus subtilis + - - + - + + + + + - - +
Bacillus thuringensis + - - - - + + + + + - - +
Enterobacter aerogenes + - - + - + + + + + - - +
Pseudomonas acidovorans + - - + - + + + + + - - +
Pseudomonas fluorescens + - - - - + + + + + - - +
Rhizobium sp + - - - - + + + + + - - +
Salmonella enteriditis + - - - - + + + + + - - +
Staphylococcus aureus + - - - - + + + + + - - +