Anais Congresso Natal

Annals
Of
2011 Symposium Organizing Committee

Presidents

Dr. Arnbio Antnio da Silva Jnior, UFRN/RN
Dra. Maria das Graas Almeida, UFRN/RN

Executive Committee

Dra. Adriana Augusto de Rezende, UFRN/RN
Dra. Aline Schwarz, UFRN/RN
Dra. Ana Paula Barreto Gomes, UFRN/RN
Dra. Ivanise Marina Moretti Rebecchi, UFRN/RN
Dra. Janana C. de Oliveira Crispim Freitas, UFRN/RN
Dr. Guilherme Maranho Chaves, UFRN/RN
Dra. Lcia Maria da Cunha Galvo, UFRN/RN

Scientific Committee

Dr. Anselmo Gomes de Oliveira, UNESP/SP
Dr. Egler Chiari, UFMG/MG
Dr. Eryvaldo Scrates Tabosa do Egito, UFRN/RN
Dr. Luiz Alberto Lira Soares, UFPE/PE
Dr. Mario Hiroyuki Hirata, USP/SP
Dr. Matheus de Freitas Fernandes Pedrosa, UFRN/RN
Dra. Raquel Brandt Giordanni, UFRN/RN
Dra. Silvana Zucolotto Langassner, UFRN/RN
Dra. Vivian Nogueira Silbiger, UFRN/RN

Scientific Evaluation Committee

Dr. Bolvar P.G.L. Damasceno, UEPB-CG/PB
Dr. Ccero Flvio Soares Arago, UFRN/RN
Dra. Cristiane C.G.O. Lopes, UFC/CE
Dra. Francilene Amaral da Silva, UFS/SE
Dr. Lvio Csar Cunha Nunes, UFPI/PI
Dr. Jos Alexsandro da Silva, UEPB-CG/PB
Dr. Jos Otvio Carrra Silva Jnior, UFPA/PA
Dra. Jlia Beatriz Pereira de Souza, UFCG-CG/PB
Dr. Najeh Maissar Khalil, UNICENTRO/PR
Dra. Paula da Silva Kujbida, UFRN/RN
Dr. Rand Randall Martins, UFCG-CG/PB
Dr. Toshiyuki Nagashima Jnior, UFCG-CG/PB
Dr. Geraldo Barroso Cavalcanti Jnior, UFRN/RN
Dra. Antnia Claudia, UFRN/RN
Dr.Tlio Moura, UFRN/RN
Dr. Anselmo Oliveira Gomes, UNESP/SP
Dr. Mrio Hiroyuki Hirata, USP/SP
Dr. Luiz Alberto Lira Soares, UFPE/PE
Dr. Matheus de Freitas Fernandes Pedrosa, UFRN/RN
Dra. Raquel Brandt Giordanni, UFRN/RN
Dra. Silvana Zucolloto Langassner, UFRN/RN
Dra. Vivian Nogueira Silbiger, UFRN/RN
Dra. Julieta Genre, UFRN/RN
Dra. Paula Machado, UFRN/RN
Dra. Maria Joselice, UFRN/RN
Dr. Euzbio Guimares Barbosa, UFRN/RN

Student Organizing Committee

Alessandro Brito dos Santos (Undergradute Pharmacy student-UFRN)
Alice Rodrigues de Oliveira (graduate student-PPGCF-UFRN)
Andra Caroline Costa de Arruda (Undergradute Pharmacy student-UFRN)
Cllio Diogo Soares (graduate student-PPGCF-UFRN)
Cristhiano Sibaldo (graduate student-PPGCF-UFRN)
Daliana Maria Berenice de Oliveira Souza (graduate student-PPGCF-UFRN)
Elyson Diego Salustino Saravia Osorio (Undergradute Pharmacy student-UFRN)
Emerson Michell da Silva Siqueira (Undergradute Pharmacy student-UFRN)
Fabiana Paiva Galvo (graduate student-PPGCF-UFRN)
Francisco Humberto Xavier Jnior (graduate student-PPGCF-UFRN)
Gabriel Araujo da Silva (graduate student-PPGCF-UFRN)
Heglayne Pereira Vital da Silva (graduate student-PPGCF-UFRN)
Ipojucan Silva Fontes (Undergradute Pharmacy student-UFRN)
Jahamunna Abrantes Andrade Barbosa (graduate student-PPGCF-UFRN)
Janaina da Silva Goes (graduate student-PPGCF-UFRN)
Julia Morais Fernandes (Undergradute Pharmacy student-UFRN)
Juliana F. Carvalho (graduate student-PPGCF-UFRN)
Juliana Flix Da Silva (Undergradute Pharmacy student-UFRN)
Julieta Genre (graduate student-PPGCF-UFRN)
Karla Samara Rocha Soares (graduate student-PPGCF-UFRN)
Karla Simone Costa de Souza (graduate student-PPGCF-UFRN)
Layany Carolyny da Silva Mouro (graduate student-PPGCF-UFRN)
Leandro Vinicius Fernandes de Morais (Undergradute Pharmacy student-UFRN)
Letcia Streck (graduate student-PPGCF-UFRN)
Lidyane Neves Miranda (graduate student-PPGCF-UFRN)
Margarete Moreno de Araujo (graduate student-PPGCF-UFRN)
Monique Christine Salgado Gomes (graduate student-PPGCF-UFRN)
Patrcia Fernandes Barbosa (Undergradute Pharmacy student-UFRN)
Polyanne Nunes de Melo (graduate student-PPGCF-UFRN)
Rafaela de Melo Pereira (graduate student-PPGCF-UFRN)
Rayllan de Oliveira Rodrigues (Undergradute Pharmacy student-UFRN)
Vitor Luiz de Brito Lemos (Undergradute Pharmacy student-UFRN)
Waleska Rayane Dantas Bezerra de Medeiros (Undergradute Pharmacy student-UFRN)
Walicyranison Plinio da Silva (graduate student-PPGCF-UFRN)
Wenna Gleyce Arajo do Nascimento (graduate student-PPGCF-UFRN)
Yamara Arruda Silva de Menezes (graduate student-PPGCF-UFRN)
Yonara Monique da Costa Oliveira (graduate student-PPGCF-UFRN)

CODE

PARTICIPANT
AUTHOR TITLE PAGE
1 Paulo Ricardo Silva Pereira PEREIRA, PRS
IMMUNOPHENOTYPIC AND FUNCTIONAL CHARACTERIZATION OF HEMATOPOIETIC STEM CELLS AFTER
MOBILIZATION IN MALNOURISHED MICE
1
2 Arthur Ladeira Macedo MACEDO, AL
IN VITRO ACARICIDAL AND CYTOTOXIC ACTIVITY OF AQUEOUS EXTRACTS OF AGAVE SISALANA PERRINE AND
FURCRAEA SELLOA K. KOCH
2
3 Renata C. Vasconcelos VASCONCELOS, RMC PRELIMARY STUDY OF BORNEOL SALICILATE ANTIINFLAMATORY ACTIVITY 3
4
Mnica Oliveira Da Silva
Simes
SIMOES MOS ESTIMATION OF CARDIOVASCULAR RISK BY THE FRAMINGHAM SCORE IN HYPERTENSIVE PATIENTS 4
5 Andr Leandro Silva SILVA, AL
CHARACTERIZATION OF A CATIONIC NANOEMULSION INTENDED TO GENE THERAPY AFTER STERILIZATION
PROCESSES
5
6 Celina Maria Pinto Guerra Dore CELINA M. P. G. DORE
SV1: ONE POLYSACCHARIDE POPULATION FROM SARGASSUM VULGARE WITH ANTICOAGULANT AND
ANTITHROMBOTIC ACTIONS AND THROMBIN INHIBITOR
6
7 Cntia R. C. Porto PORTO, CRC ANTI-INFLAMMATORY AND ANTINOCICEPTIVE ACTIVITIES OF PHYLLANTHUS NIRURI L SPRAY-DRIED EXTRACT 7
8 Fernanda Rodrigues Salazar SALAZAR, FR
DETERMINATION OF RALOXIFENE HYDROCHLORIDE USING ULTRAVIOLET SPECTROPHOTOMETRY AND LC
STABILITY-INDICATING METHOD
8
10
Andressa Santa Brigida Da
Silva
SILVA, ASB AQUEOUS EXTRACT OF MIKANIA LINDLEYANA DC. PRESENTS POTENTIAL ANTI-INFLAMMATORY EFFECT 9
12 Ana Carolina Kogawa KOGAWA, A.C. DEVELOPMENT AND VALIDATION OF DOXYCYCLINE HYCLATE BY HPLC METHOD 10
13 Anderson Angel Vieira Pinheiro PINHEIRO, A.A.V.
STRATEGY FOR TREATMENT OF WATER, FEDERAL UNIVERSITY OF CAMPINA GRANDE / CENTER FOR
EDUCATION AND HEALTH-PB
11
14
urea Marcela De Souza
Pereira
PEREIRA, A.M.S. ADVERSE EVENTS IDENTIFIED IN A HOSPITAL OF THE SENTINEL NETWORK 12
15 Brbara Coeli SILVA, B. C. O STANDARDIZATION OF TECHNIQUES FOR EVALUATION OF HEMATOPOIESIS IN MALNOURISHED MICE 13
16 Clarissa Nunes De Assis ASSIS, C. N. REFLECTIONS OF A CUSTODY CHAIN IN CENTER OF PHARMACEUTICAL EQUIVALENCE 14
17 Dbora Freitas Do Nascimento NASCIMENTO, D.F.
NANOCOSMETICS IN PHOTOPROTECTION: DEVELOPMENT AND CHARACTERIZATION OF NANOPARTICLES
WITH SUNSCREENS
15
20 Margareth Linde Athayde ATHAYDE, M. L. ANTIMYCOBACTERIAL ACTIVITY OF THE FRACTIONS AND COMPOUNDS FROM SCUTIA BUXIFOLIA REISSEK 16
21.1 Monique Gabriela ALVES, M. G. C. F
DIRECT INHIBITORY ACTION OF SULFATED POLYSACCHARIDES FROM RED SEAWEED HYPNEA MUSCIFORMIS
ON THROMBIN ENZYMATIC ACTIVITY EVALUATED BY CHROMOGENIC SUBSTRATE ASSAY
17
21.2 Monique Gabriela ALVES. M. G. C. F
INHIBITORY ACTION ON LIPID PEROXIDATION AND PROTECTIVE ACTIVITY ON H2O2-INDUCED HEMOLYSIS OF
SULFATED POLYSACCHARIDES F1.0 FROM RED SEAWEED HYPNEA MUSCIFORMIS
18
23 Nially Karen Morais Do Amaral
NENHUM AUTOR
SUBLINHADO
DETERMINATION OF ACIDITY IN CURD CHEESE OF CAIC AND JUCURUTU 19
II INTERNATIONAL SYMPOSIUM ON PHARMACEUTICAL SCIENCES:
Challenge and opportunities for development new diagnosis methods and pharmaceutical products

ABSTRACTS
24 Adalgiza D. V. Silva SILVA. A. D. V. TOXICOLOGIC ASSAY OF ETHANOLIC EXTRACT FROM XYLOPIA CAYENNENSIS 20
25
Everton Do Nascimento
Alencar
ALENCAR. E. N ANTIMICROBIAL ACTIVITY EVALUATION OF COPAIBA OIL MICROEMULSION SYSTEM: A PRELIMINARY STUDY 21
26
Julius Roberto Martins De
Oliveira
OLIVEIRA. J. R. M. DE PROFILE AND QUALITY OF MEDICATIONS PRESCRIPTION IN THE CITY OF CUIT PB. BRAZIL 22
27.1 Mallone Lopes Da Silva
NENHUM AUTOR
SUBLINHADO
ANLISE FITOQUMICA E CROMATOGRFICA DE MIKANIA GLOMERATA 23
27.2 Mallone Lopes Da Silva
NENHUM AUTOR
SUBLINHADO
INVESTIGATION OF METABOLITES FROM XYLOPIA CAYANNENSIS 24
28 Rosilene Rodrigues Santiago SANTIAGO. R. R. ZINC OXIDE EMULSION: A NEW ALTERNATIVE TO DIAPER DERMATITIS TREATMENT 25
30 Almria Mariz Batista BATISTA. A. M.
LEGAL ANALYSIS OF MEDICINES ADVERTISING TRANSMITTED IN RADIO STATION FROM NATAL/RN
ACCORDING TO RDC N 96/08
26
31.1 Rosalina Coelho Jcome JCOME. R. C
STUDY OF ISONIAZID ADSORPTION ONTO CHITOSAN: ASSAYS OF EQUILIBRIUM AND KINETICS USING STIRRED
TANK
27
31.2 Rosalina Coelho Jcome JCOME. R. C HOUSEHOLD SURVEY ABOUT THE PROFILE OF DRUG USE BY ELDERLY IN THE CITY OF CUIT-PB 28
32 Cristhiano Sibaldo ALMEIDA. C.S.
ORAL ADMINISTRATION OF EXTRACT CYANOBACTERIAL BLOOM IN PREGNANT RATS: A REPRODUCTION
ASSESSMENT
29
33
Daisy Dayane Formiga De
Souza Lima
LIMA. D.D.F.S
COMPARATIVE ANLYSIS OF PHYSICAL AND CHEMICAL PROPERTIES OF TWO SOURCES OF WATER FOR USE
ON SCHOLL PHARMACY MANUEL CASADO ALMEIDA. CUIT-PB
30
34.1 Eliane Gandolpho Ttoli TTOLI. E. G. QUALITATIVE TESTS OF AMPICILLIN SODIUM POWDER FOR INJECTION 31
34.2 Eliane Gandolpho Ttoli TTOLI. E. G.
DEVELOPMENT AND VALIDATION OF TURBIDIMETRIC ASSAY TO POTENCY EVALUATION OF AMPICILLIN
SODIUM FOR INJECTION
32
35 Ivson Medeiros MEDEIROS JR. I. J.
TECHNICAL AND ADMINISTRATIVE MANAGEMENT OF SCHOOL PHARMACY'S WAREHOUSE MANOEL CASADO
DE ALMEIDA UFCG/CES.
33
36
Jose Marcos Dos Santos
Oliveira
OLIVEIRA. J. M. S.
VALIDATION OF A SPECTROPHOTOMETRIC METHOD (UV-VIS) FOR DETERMINATION OF TOTAL FLAVONOIDS OF
THE RED PROPOLIS FROM ALAGOAS: USE OF CATECHIN AS BIOMARKER
34
37.1 Keine Monteiro Castro CASTRO K.M. CONSTITUENTS STUDY OF FIXED PIKE NORTHEAST ( CARYOCAR CORIACEUM WITTMER.) 35
37.2 Keine Monteiro Castro CASTRO K.M.
GUIDANCE ON PHARMACEUTICAL PREPARATIONS MEDICINAL PLANTS WITHHOMEMADE: A STRATEGY
FOR FAMILY HEALTH
36
38 Larice Brito De Vasconcelos VASCONCELOS. L. B.
STUDY OF THE TOXICITY OF ESSENTIAL OIL OF CYMBOPOGON CITRATUS (DC) STAF. FOR A POSSIBLE
APPLICATION AS ANTIFUNGAL DRUG
37
39.1 Luis Fabio Dos Santos Gomes GOMES.L.F.S. PRELIMINARY STUDY OF THE TOXICITY OF ASPIDOSPERMA EXCELSUM BENTH (APOCYNACEAE). 38
39.2 Luis Fabio Dos Santos Gomes GOMES.L.F.S.
ACUTE ORAL TOXICITY EVALUATION OF THE DRY ETHANOLIC EXTRACT OF HIMATANTHUS ARTICULATUS AND
ELEUTHERINE PLICATA
39
40.1 Mayara Ladeira Coelho COLHO. M. L.
HISTOPATHOLOGICAL STUDIES OF THE EFFECTS OF THE AQUEOUS EXTRACT OF THE ORBIGNYA PHALERATA
MART (BABASSU) MESOCARP POWDER IN ADULT MICE TREATED ORALLY
40
40.2 Mayara Ladeira Coelho COLHO. M. L.
THERMAL CHARACTERIZATION OF BABASSU (ORBIGNYA PHALERATA MART) MESOCARP POWDER COMPARED
WITH THE RAW MATERIAL MICROCRYSTALLINE CELLULOSE
41
41
Severina Denise Sales De
Oliveira
NENHUM AUTOR
SUBLINHADO
STUDY AND CHARACTERIZATION SPECTROSCOPIC OF THE COMPLEX POTASSIUM HEXACYANOFERRATE 42
42 Tassia Tatianna Firmino FIRMINO. T.T.D.
MEASUREMENT OF TOTAL FLAVONOIDS IN THE HYDROALCOHOLIC EXTRACTS OF RED PROPOLIS FROM
ALAGOAS
43
43 Yohana Souza Pinto PINTO. Y.S.
COMPARISON OF METHODS FOR DETERMINATION OF CONTENT PROTEIN IN THE AQUEOUS AND ACETONE
EXTRACT ANNONA SQUAMOSA
44
44 Alaine Maria Dos Santos Silva SILVA. A.M.S
PHYSICO-CHEMICAL PARAMETERS OF LANETTE AND POLAWAX EMULSIONS FOR MANOEL CASADO DE
ALMEIDA/UFCG. PB PHARMACY SCHOOL PRODUCTION
45
45 Clarissa Nunes De Assis ASSIS. C. N
MICROBIOLOGICAL ANALYSIS OF WATER FROM DIFFERENT SOURCES OF FORTALEZA IN THE PERIOD
JANUARY TO SEPTEMBER 2011
46
46 Lisllie Dayse Arajo Santos SANTOS. L.D.A
UTILIZATION OF MG. AL LAYERED DOUBLE HYDROXIDES (LDHS) AS A CARRIER OF DIGOXINE FOR
VECTORIZATION IN CHEMOTERAPY OF CANCER
47
47 Livaldo Costa E Silva
SILVA. LIVALDO COSTA
E
CREATION OF DIGITAL'S MAGAZINES ON RATIONAL USE OF DRUGS: NEW PERSPECTIVES ON EDUCATION 48
48 Marana Silveira Ali ALI. M.S.
CHRONIC. BUT NOT ACUTE. ETHANOL WITHDRAWL AFTER CHRONIC CONSUMPTION PROMOTES DEPRESSIVE-
LIKE BEHAVIOUR WITHOUT ALTERING LOCOMOTION IN RATS
49
49.1 Rayanne Karen Cunha Gurgel GURGEL. R. K. C
APPLICATION OF THERMOANALYTICAL METHODS IN EVALUATING THE BEHAVIOR OF TUBERCULOSTATICS
DRUGS PRODUCED BY NUPLAM / UFRN: ISONIAZID AND PYRAZINAMIDE
50
49.2 Rayanne Karen Cunha Gurgel GURGEL. R. K. C
DETERMINATION OF PHYSICAL AND CHEMICAL COMPOSITION FOR THE QUALITY CONTROL OF MILK
CONSUMED IN THE CITY OF NATAL / RN
51
50
Lorena Cit Lopes Resende
Santana
SANTANA. LCLR.
STUDY OF PHYSICOCHEMICAL PROPERTIES AND ACUTE TOXICITY EVALUATION OF ETHANOLIC EXTRACT OF
THE LEAVES FROM MIKANIA GLOMERATA SPRENGEL.
52
51 Nayara Maciel Mendona MENDONA. N.M EPIDEMIOLOGY OF CANDIDEMIA IN PUBLIC HOSPITALS OF ALAGOAS. BRASIL 53
52.1
Andria Cristina Wildner
Campos Lopes
WILDNER A. C.
VALIDATION OF ANALYTHICAL METHODOLOGY FOR PASSIFLORA INCARNATA L. EXTRACT BY HPLC AND
ANALYISIS OF COMMERCIALS EXTRACTS FROM RS
54
52.2
Andria Cristina Wildner
Campos Lopes
MENEGHINI. LC
DEVELOPMENT AND VALIDATION OF A METHOD OF DISSOLUTION FOR DARIFENACIN EXTENDED-RELEASE
TABLETS BASED ON IN VIVO DATA
55
53.1
Luiza Sheyla Evenni Porfrio
Will
WILL. L.S.E.P. LOBOPHORA VARIEGATA AND ITS ACTIVITY IN PAW EDEMA AND HEPATOTOXICITY IN RATS 56
53.2
Luiza Sheyla Evenni Porfrio
Will
WILL. L.S.E.P.
-GLUCANS OF CARIPIA MONTAGNEI MUSHROOM SHOWS ANTI-INFLAMMATORY ACTIVITY: NEWS
PERSPECTIVE OF PPAR ATION
57
54 Maria Tatiane Dias Moura
NENHUM AUTOR
SUBLINHADO
EVALUATION OF HYPOTENSIVE RESPONSES OF LYOPHILIZED AYAHUASCA TEA IN SHR RATS 58
55.1 Marilia Da Silva Nascimento NASCIMENTO. M.S. ANGIOGENESIS INHIBITION AND B-GLUCANS ANTITUMOR ACTION OF CARIPIA MONTAGNEI MUSHROOM 59
55.2 Marilia Da Silva Nascimento NASCIMENTO. M.S.
CARIPIA MONTAGNEI MUSHROOM B-GLUCANS SHOWING NON HEMOLYTIC ACTION AND ANTIOXIDANT
ACTIVITY: POTENTIAL FUNCTION IN LIPIDIC PEROXIDATION INHIBIT AND OXIDATIVE STRESS
60
56 Luciane De Lira Teixeira
NENHUM AUTOR
SUBLINHADO
DETERMINATION OF MOISTURE AND LIPIDS IN FRUITS OF LICANIA TOMENTOSA BETH (OITI) 61
57 Prycila Rodrigues Feitoza
NENHUM AUTOR
SUBLINHADO
EVALUATION OF THE CARDIOVASCULAR EFFECTS OF SYZIGIUM CUMINI SEEDS 62
58.1 Thuane De Sousa Pinheiro PINHEIRO. T.S. POLYSACCHARIDES EFFECTS FROM LOBOPHORA VARIEGATA IN A TOPICAL INFLAMMATORY MODEL 63
58.2 Thuane De Sousa Pinheiro PINHEIRO. T.S. ANTIOXIDANT ASSAYS OF BRUTE EXTRACTED FROM PLECTTRANTUS BARBATUS ANDR. 64
59 T T SILVA JNIOR. J.O.C.
EVALUATION OF DENTIN ROUGHNESS AFTER USE OF DIFFERENTS CHELATING AGENTS EMPLOYED IN
ENDODONTICS
65
61 Mariana De Macedo Costa COSTA. M.M
TOPICAL APPLICATION OF PROPOLIS FOR HEALING OF WOUNDS SURGICALLY INDUCED AND EVALUATION OF
BIOCHEMICAL PARAMETERS IN WISTAR RATS
66
62 Mrcia Natlia Da Silveira Pinto PINTO. M. N. S EVALUATION CYTOTOXIC IN VITRO OF NEW COMPOUND LPSF/MS-02 67
63 Roberta Da Silva Campos CAMPOS. R.S. DETERMINATION OF PROTEIN CONTENT OF ZIZYPHUS JOAZEIRO 68
64
Thales Allyrio Arajo De
Medeiros Fernandes
FERNANDES. T.A.A.M. COMPARISON OF LABORATORY CHARACTERISTICS OF PATIENTS WITH SICKLE CELL DISEASE 69
65.1 Tahisa Marcela Pedroso
NENHUM AUTOR
SUBLINHADO
UV SPECTROPHOTOMETRIC PROFILE OF CEFAZOLIN SODIUM 70
65.2 Tahisa Marcela Pedroso
NENHUM AUTOR
SUBLINHADO
A NEW. SAFE AND EASY METHOD OF THIN-LAYER CHROMATOGRAPHY FOR CEFAZOLIN SODIUM
IDENTIFICATION
71
66 Jos Couras Silva Filho SILVA-FILHO. J. C.
VOLTAGE-OPERATED POTASSIUM CHANNELS ARE INVOLVED IN (-)-BORNEOL-INDUCED VASORELAXANT
RESPONSE ON RAT MESENTERIC ARTERY
72
67 Katyane Rodrigues RODRIGUES. K. PREPARATION OF NANOSTRUCTURED SYSTEMS SUNSCREENS ORGANIC INORGANIC 73
68 Luza Xavier XAVIER. L.A ANTIBACTERIAL EVALUATION OF NEW DERIVATIVES OF THIOPHENE 74
69 Camila Alves De Moura MOURA.C.A THEORETICAL AND PRACTICAL HANDBOOK: A TOOL FOR THE TRAINEESHIP LIVE PHARMACIES 75
70
Elyson Diego Salustino Saravia
Osorio
OSORIO. E. D. S. S. DEVELOPMENT OF STRATEGIES OF MOLECULAR DIAGNOSTIC APPLIED TO DYSLIPIDEMIA 76
71 Flvia Danniele Frota Machado MACHADO. F.D.F
EVALUATION OF THE ROLE OF THE NITRIC OXIDE IN GASTROPROTECTIVE ACTIVITY OF PARTITION
FRACTIONS OF NEOGLAZIOVIA VARIEGATA MEZ. (BROMELIACEAE) IN MODEL OF ULCERS INDUCED BY
ETHANOL
77
72 Izadora De Souza DE SOUZA. I. OPTIMIZATION OF AN EXTRACTION PROCEDURE FOR THE BIOPOLYMER CHITIN 78
73.1
Danielle Cristina Zimmermann
Franco
ZIMMERMANN-
FRANCO. D. C.
USE OF POTENTIALLY INAPPROPRIATE MEDICATIONS AND THE RISK OF POSSIBLE DRUG INTERACTIONS
BETWEEN INSTITUTIONALIZED FRAIL ELDERLY IN A CITY IN THE ZONA DA MATA MINEIRA
79
73.2
Danielle Cristina Zimmermann
Franco
ZIMMERMANN-
FRANCO. D. C.
ANTIFUNGAL ACTIVITY OF OLEORESIN EXTRACTED FROM COPAIFERA LANGSDORFFII DESF AGAINST
DERMATOPHYTES FUNGI
80
74 Margarete Moreno De Araujo DE ARAUJO. M. M.
EVALUATION OF ACCELERATED STABILITY OF SOYBEAN O/W EMULSION CONTAINING ETHANOL AND BENZYL
ALCOHOL AS COSURFACTANTS
81
75
Mariana Arajo Paulo De
Medeiros
MEDEIROS. M. A. P
EVALUATION OF MORPHOGENESIS AND PROTEINASE ACTIVITY OF CLINICAL ISOLATES OF C. ALBICANS
OBTAINED FROM PATIENTS WITH VULVOVAGINAL CANDIDIASIS
82
76 Cristiane Franco Codevilla CODEVILLA C.F. DETERMINATION OF LODENAFIL CARBONATE USING CAPILLARY ZONE ELECTROPHORESIS METHOD 83
77
Melissa Alexandra Da Rosa
Costa
COSTA. M .A. R.
ANALYSIS OF EPIDEMIOLOGICAL SITUATION OF LEPROSY IN CHILDREN UNDER 15 YEARS OLD IN FORTALEZA
IN THE PERIOD OF 2001 TO 2010
84
78.1 rika Yoko Suzuki SUZUKI. E.Y IN VITRO INHIBITION OF CLINICAL PSEUDOMONAS AERUGINOSA ADHESION BY XYLITOL 85
78.2 rika Yoko Suzuki SUZUKI. E.Y ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL FROM BACCHARIS TRIMERA (LESS) DC 86
80 Daiane Soares SOARES. D.S PHYSICAL-CHEMICAL COMPATIBILITY BETWEEN ATTAPULGITE. RIFAMPICIN AND ISONIAZID 87
81 Elquio Eleamen Oliveira MARCELINO. H. R.
NEW METHOD TO PRODUCE XYLAN MICROPARTICLES USING SODIUM TRIMETAPHOSPHATE AS CROSS-
LINKING AGENT
88
82 Jssica Guedes GUEDES. J. S. SYNTHESIS OF TIN (IV) COMPLEXES WITH POTENTIAL ANTIMICROBIAL ACTIVITY 89
83
Jseffer Wianey Trindade
Medeiros
MEDEIROS. J. W. T. ADVERSE DRUG REACTIONS AS A CAUSE OF HOSPITAL ADMISSION 90
84.1 Juliana Flix Da Silva FLIX-SILVA. J
ANTI-INFLAMMATORY ACTIVITY OF AQUEOUS LEAF EXTRACT OF JATROPHA GOSSYPIIFOLIA L. IN
CARRAGEENIN-INDUCED PAW EDEMA IN RATS
91
84.2 Juliana Flix Da Silva FLIX-SILVA. J
ANTI-INFLAMMATORY ACTIVITY OF BULLFROG (RANA CATESBEIANA) OIL IN CARRAGEENIN INDUCED PAW
EDEMA IN RATS
92
86 Maria Luiza Gois Campos CAMPOS. M. L. G INCLUSION COMPLEXES OF FUROSEMIDE WITH -CYCLODEXTRIN AND PVP 93
87.1
Nathlia Alexandra De Oliveira
Cartaxo
CARTAXO. N.A.DE O. RATIONAL USE OF DRUGS IN HYPERTENSION: AN ACHIEVEMENT OF PHARMACEUTICAL CARE 94
87.2
Nathlia Alexandra De Oliveira
Cartaxo
CARTAXO. N.A.DE O.
EXTRACTION OF FLAVONOIDS AND POLYPHENOLS IN LEAVES OF MENTHA X PIPERITA L. PROCESSED IN
FIXED BED CONVECTIVE DRYER
95
90
Andressa Noronha Barbosa Da
Silva
SILVA. A. N. B.
DISTRIBUTION OF TRIATOMINES FAUNA AND NATURAL INFECTION BY TRYPANOSOMA CRUZI IN THE SEMIARID
OF THE STATE OF RIO GRANDE DO NORTE. BRAZIL
96
91 Michelle De Oliveira Pedrosa PEDROSA. M.O. DEVELOPMENT OF THIOPHENES DERIVATIVES BIOCOMPATIBLES MICROEMULSIONS 97
92 Karolyne Dos Santos Silva SILVA. K. S INFLUENCE OF PLURONIC F68 ON THE BENZATHINE PENICILLIN G ANTIBACTERIAL ACTIVITY 98
93
Myra Furtado Dias Filgueira
Th
TH. M. F. D. F.
EVALUATION OF THE ANTIFUNGAL ACTIVITY OF NOR-LAPACHONE DERIVATIVE AGAINST ISOLATES OF
CANDIDA SPP.
99
94
Jacyra Antunes Dos Santos
Gomes
GOMES. J. A. S.
QUALITATIVE PHYTOCHEMICAL PROFILE OF JATROPHA GOSSYPIIFOLIA L. EXTRACTS PREPARED BY
DIFFERENT METHODS OF EXTRACTION
100
95.1
Magda Rhayanny Assuno
Ferreira
FERREIRA. M. R. A
EVALUATION OF ANTIFUNGAL ACTIVITY OF CRUDE EXTRACT FROM CAESALPINIA FERREA MART AGAINST
CLINICAL ISOLATES FROM THE ORAL CAVITY OF RENAL TRANSPLANTATION
101
95.2
Magda Rhayanny Assuno
Ferreira
FERREIRA. M. R. A
BIOASSAY-GUIDED PURIFICATION OF CRUDE EXTRACT FROM STEM BARKS FROM CAESALPINIA FERREA
MART (PAU FERRO)
102
96 Walicyranison Plinio Da Silva SILVA. W.P
IDENTIFICATION AND PHOSPHOLIPASE ACTIVITY OF FUNGI ISOLATED FROM DERMATOMYCOSES OF
CHILDREN ATTENDING MUNICIPAL CENTERS OF CHILDREN EDUCATION FROM NATAL-RN
103
97 Patrcia Barbosa BARBOSA. P.F. EVALUATION OF THE REPRODUCTIVE AND FETAL TOXICITY OF CYLINDROSPERMOPSIN IN THE RAT 104
98.1
Maria Aparecida Alves Leite
Dos Santos Almeida
ALMEIDA. M.A.A.L.S
DEVELOPMENT OF A LIQUID DETERGENT FORMULATION FOR USE IN UFCG/CES AND EVALUATION OF
VISCOSITY AND POWER FOAM
105
98.2
Maria Aparecida Alves Leite
Dos Santos Almeida
ALMEIDA. M.A.A.L.S
RETROSPECTIVE STUDY OF ADVERSE DRUG REACTIONS IN THE PEDIATRIC WARD OF THE UNIVERSITY
HOSPITAL ALCIDES CARNEIRO. CAMPINA GRANDE / PB
106
99 Alexandre Medeiros Dantas OLIVEIRA. A. R. IN VITRO RELEASE OF METHOTREXATE-LOADEDSPRAY DRIED PLGA MICROPARTICLES 107
101 Letcia Streck STRECK. L. BENZNIDAZOLE-LOADED SOYBEAN O/W EMULSION SYSTEM 108
102 Milene Evelyn Da Silva
NENHUM AUTOR
SUBLINHADO
EVALUATION OF CITRONELLA (CYMBOPOGON WINTERIANUS JOWITT EX BOR.) OIL COMPOSITION EXTRACTED
FRESH AND DRY PLANT AND INFLUENCE OF HARVESTING PERIOD
109
103
Cynthia Layse Ferreira De
Almeida
ALMEIDA. C. L. F.
SCREENING FOR ANTIBACTERIAL ACTIVITY ANTIMICROBIAL ACTIVITY OF HYDROALCOHOLIC EXTRACT OF
ANADENANTHERA MACROCARPA BENTH AND MYRACODRUOM URUNDEUVA ALL. AGAINST STRAIN OF
STAPHYLOCOCCUS AUREUS
110
104 Anne Kaliery De Abreu Alves ALVES. A.K.A. OUABAIN REDUCES THE PHAGOCYTOSIS OF PERITONEAL MACROPHAGES 111
105 Manuelle Prestrelo De Oliveira
NENHUM AUTOR
SUBLINHADO
EVALUATION OF THE RELAXING ACTIVITY OF ETHANOL EXTRATCT FROM LEAVES ASPIDOSPERMA
MACROCARPUM IN ISOLATED RAT MESENTERIC ARTERY
112
106 Luis Paulo Da Penha Ferino FERINO. L.P.P. EVALUATION OF TESTS PRE-TRANSFUSIONAL IMMUNO-HEMATOLOGICAL IN RECEPTORS OF BLOOD 113
107 Ricardo Bizogne Souto SOUTO R. B.
DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE LIQUID CHROMATOGRAPHY METHOD FOR THE
EVALUATION OF RECOMBINANT HUMAN INTERLEUKIN-11. CORRELATION WITH THE BIOASSAY
114
108 Fernanda Pavani Stamm STAMM. F. P
STUDY OF A RP-LC METHOD FOR THE ANALYSIS OF RECOMBINANT PARATHYROID HORMONE IN
BIOPHARMACEUTICAL FORMULATIONS
115
109 Polyanne Nunes De Melo MELO. P. N.
EFFECT OF TRIETHANOLAMINE/METHYL-1-PYRROLIDONE-2/BETACYCLODEXTRIN MULTICOMPONENT
COMPLEXES SYSTEMS ON THE BENZNIDAZOLE APPARENT SOLUBILITY
116
111.1 Carolina Moraes Cab CAB. C. M
PHYSICAL PARAMETERS OF QUALITY ASSESSMENT OF DIFFERENT LOTS OF HOMEOPATHIC GLOBULES IN
USE IN NATAL /RN/BRAZIL
117
111.2 Carolina Moraes Cab CAB. C. M SPECTRAL ANALYSIS (FT-IR. UV) OF RELEASE OF LEACHABLES FROM NATURAL RUBBER BULBS 118
112
Horacinna Maria De Medeiros
Cavalcante
CAVALCANTE. H. M. M. TOXICITY OF DENTAL VARNISH CHITOSAN 119
113 Edeildo Ferreira SILVA JNIOR. E. F.
NEW TALENT PROGRAM EDUCATION FOR HEALTH ANALYSIS AND EDUCATION IN WATER USE IN THE OF
TRAIPU ALAGOAS
120
114
Katharina Rodrigues De Lima
Porto Ramos
RAMOS. K. R. L. P. EVALUATION OF THE CARDIOVASULAR RISK IN CONNECTION TO C-REACTIVE PROTEIN 121
115
Antnio Vincius Doriguetto
Ferreira
FERREIRA. A. V. D
CHEMICAL PROFILE VERIFICATION OF THE EXTRACTS AND SEMI-PURIFIED FRACTIONS OF ANDIRA NITIDA
MART. EX BENTH BY TLC
122
116
Leonardo Augusto Rgo De
Souza
NENHUM AUTOR
SUBLINHADO
PRO-INFLAMMATORY AND CYTOTOXIC ACTION CARRAGEENAN OF THE SEAWEED AMANSIA MULTIFIDA 123
117.1 Geovani Pereira Guimares GUIMARES. G. P ANTIFUNGAL ACTIVITY OF MEDICINAL PLANTS AGAINST CANDIDA SPECIES 124
117.2 Geovani Pereira Guimares GUIMARES. G. P DEVELOPMENT OF A LSVM-5CN05 TRANSDERMIC MICROEMULSION 125
118
Mallu Hemmer Gomes De
Menezes
MENEZES. M.H.G. ACUTE AND SUBACUTE TOXICITY OF CAESALPINIA FERREA STEM BARK EXTRACT 126
119.1
Paula Regina Rodrigues
Salgado
SALGADO. P. R. R.
ANTINOCICEPTIVE ACTIVITY OF CURINE ON THE ACETIC ACID ABDOMINAL CONSTRICTION TEST (WRITHING
TEST) IN MICE
127
119.2
Paula Regina Rodrigues
Salgado
SALGADO. P. R. R.
EVALUATION OF THE ANXIOLYTIC EFFECT OF ETHANOLIC EXTRACT OF SIDASTRUM MICRANTHUM
(MALVACEAE) ON THE ELEVATED PLUS-MAZE TEST IN MICE.
128
122 Severino Antnio De Lima Neto VARGAS. M. R. W. DE
SCANNING ELECTRON MICROSCOPY (SEM) / ENERGY-DISPERSIVE X-RAY SPECTROSCOPY (EDS) AS AN
METHOD FOR THE CHARACTERIZATION OF MICROPARTICLES.
129
123 Gabriel Chaves Neto CHAVES NETO. G1 CLINICAL MODELS AND EVALUATION MEASURES IN THE STUDY OF EXPERIMENTAL ANXIETY 130
125 Ademar Soares Melo MELO. A.S
EVALUATION OF THE TYPE ACTION ANTIDEPRESSANT OF THE EXTRACT HYDROALCOHOLIC OF EUPATORIUM
AYAPANA VENT.
131
126
Yamara Arruda Silva De
Menezes
MENEZES. Y. A. S.1 EXTRACTION. MEASUREMENT AND ANALYSIS OF TOTAL PROTEIN OF NATIVE PLANTS FROM RN 132
128 Mayara Marilia Ferreira Limeira LIMEIRA. M.M.F. REPEAT-DOSE TOXICITY OF THE AQUEOUS EXTRACT OF CAESALPINIA FERREA STEM BARK 133
129 Marco Navarro NAVARRO. M. V. M.
PRELIMINARY STUDIES FOR THE USE OF ARTIFICIAL NEURAL NETWORKS (ANN) IN THE STUDY OF RHEOLOGIC
PROPERTIES OF TUBERCULOSTATICS AND SOLID EXCIPIENTS
134
130 Alexandre Medeiros Dantas DANTAS. A.M.
GASTROPROTECTIVE EFFECTS OF ETHANOLIC EXTRACT FROM SPONDIA PURPUREA ON EXPERIMENTAL
GASTRIC ULCER MODELS IN RODENTS
135
131 Romlia Pinheiro Gonalves BANDEIRA. I.C.J
IMPACT ON HEMATOLOGICAL PARAMETERS OF L-ARGININE SUPPLEMENTATION IN PATIENTS WITH SICKLE
CELL ANEMIA IN USE OF HYDROXYUREA
136
132 Izabel Cristina Justino Bandeira BANDEIRA. I.C.J
ALTERAES HEMATOLGICAS EM CRIANAS COM TETRALOGIA DE FALLOT SUBMETIDAS CIRURGIA
CARDACA COM CIRCULAO EXTRACORPREA
137
133 Thiago Peixoto Rodrigues RODRIGUES. T.P
PROFILE OF PATIENTS WITH CHRONIC DISEASE NON-TRANSMISSIBLE PARTICIPANTS OF A PHARMACEUTICAL
CARE PROGRAM
138
134
Gabriel Azevedo De Brito
Damasceno
DAMASCENO GAB
PREPARATION AND CHARACTERIZATION OF INCLUSION COMPLEXES BETWEEN CHLORPROPAMIDE AND -
CYCLODEXTRIN
139
135
Maria Aline Barros Fidelis De
Moura
MIRANDA. N. TOXICOLOGICAL PROFILE OF EMERGENCY ATTENDED AT VIOSA-AL HOSPITAL 140
136 Gabriel Araujo Da Silva ARAUJO-SILVA. G.
DEVELOPMENT AND VALIDATION OF HPLC FAST METHOD FOR SIMULTANEOUS DETERMINATION OF NINE
POLYPHENOLS COMPOUNDS IN CRUDE EXTRACTS OF PLANTS
141
137.1 Denise Michelle Costa Barros BARROS. D.M.C.
APPLICATION OF DIFFERENTIAL THERMAL ANALYSIS (DTA) TO DETERMINE OF TRIOXSALEN-EXCIPIENT
COMPATIBILITY
142
137.2 Denise Michelle Costa Barros BARROS. D.M.C. COMPATIBILITY STUDY OF VITAMIN D3 AND MANNITOL IN PHYSICAL MIXTURES 143
138 Jonas Ivan Nobre Oliveira OLIVEIRA. J. I. N
A QUANTUM STUDY OF DRUG-RECEPTOR INTERACTION: RECONHECENDO AS BINDING ENERGIES BETWEEN
16S RRNA RESIDUES AND HYGROMYCIN B
144
140
Yonara Monique Da Costa
Oliveira
OLIVEIRA. Y.M.C
STUDY OF 599 C>T POLYMORPHISM OF THE GPX1 GENE IN TYPE 1 DIABETES: IMPLICATIONS IN ANTIOXIDANT
DEFENSE AND DIABETIC NEPHROPATHY
145
141.1 Thereza Mylene PEREIRA. T. M. M PRELIMINARY STUDY FOR THERMAL CHARACTERIZATION OF BIOIDENTICAL HORMONE ESTRADIOL 146
141.2 Thereza Mylene PEREIRA. T. M. M
LINEARITY IN DTA METHOD: A COMPARISON OF TWO QUANTIFICATION STRATEGIES OF BIOIDENTICAL
HORMONE
147
142 Walte Louis Lima Da Silveira SILVEIRA. W. L. L. INTERFERENCE ON ADHERENCE OF CANDIDA SSP IN CONTACT LENSES BY MICROEMULSION SYSTEMS 148
143.1 Thays Oliveira OLIVEIRA. T.S. CHARACTERIZATION OF THERMAL EVENTS OF HYDROQUINONE DEPIGMENTANT BY DTA 149
143.2 Thays Oliveira OLIVEIRA. T.S. COMPATIBILITY STUDY OF TRETINOIN AND EMULSIFYING AGENT IN PHYSICAL MIXTURES 150
144 Fabrcio Alexopulos Ferreira MELO. A.S.
EVALUATION OF THE ANTINOCICEPTIVE EFFECT OF HYDROETANOLIC EXTRACT OF PORTULACA PILOSA L
(PORTULACACEAE). IN MICE
151
145 Mara Rbia Winter De Vargas VARGAS. M. R. W
EVALUATION OF PHYSICOCHEMICAL PROPERTIES OF POORLY WATER SOLUBLE DRUG (SIMVASTATIN)
OBTAINED BY RECRYSTALLIZATION
152
146 Erlhane Irineu Loureno Silva SILVA; E.I.L ANALYSIS AND CHARACTERIZATION OF OILS AND FATS 153
148
Franklin Ferreira De Farias
Nbrega
NBREGA. F.F.F.
EVALUATION OF ANTIOXIDANT ACTIVITY IN SEASONAL STUDY OF PHASE ETHYL ACETATE BAUHINIA
CHEILANTHA (BONG.) STEUDEL
154
149.1
Renata Cristina De Araujo
Valena
VALENA. R.C.A PHARMACEUTICAL ASSISTANCE THE ELDERLY: SHARES OF COMMUNITY INTERVENTION IN BRASILIA TEIMOSA 155
149.2
Renata Cristina De Araujo
Valena
VALENA. R.C.A PROTEIN ANALYSIS BY BRADFORD METHOD IN SAMPLES OF CEREAL 156
150 Gabriela Muniz Felix Arajo ARAJO. G. M. F.
ANTIMICROBIAL ACTIVITY OF HYDROALCOHOLIC EXTRACT OF STRYPHNODENDRON ADSTRINGENS MART.
AGAINST STRAIN OF STAPHYLOCOCCUS AUREUS
157
151 Ktia Lira Da Silva SILVA. K. L.
STUDIES OF MAGNETIC PARTICLES PREPARED BY CO-PRECIPITATION UNDER MAGNETIC FIELD FOR DRUG
DELIVERY
158
152
Jssica Layanne Cabral
Azevedo
AZEVEDO. J. L. C A STRUCTURAL AND ENERGETIC INVESTIGATION OF PAROMOMYCIN-16S RRNA COMPLEX 159
153 Joo Ricardo P. De Carvalho CARVALHO. J.R.P
SYNTHESIS AND CHARACTERIZATION OF AMOXICILLIN AND METRONIDAZOLE-BASED CHITOSAN
MICROPARTICLES
160
154 Tulio Salgado SALGADO. T.M.M VISCERAL LEISHMANIASIS: SURVEY OF NOTIFIED CASES OF DISEASE IN THE CITY OF ASSU / RN 161
156 Wendell M. De Azevedo. AZEVEDO. W. M. DE
OZAWA CALCULATION APPLIED TO THE KINETIC STUDY OF DECOMPOSITION OF FLUCONAZOLE BY
THERMOGRAVIMETRY
162
157.1 Edilamar Pereira Da Silva SILVA E. P. THERMAL CHARACTERIZATION OF ALKALOID SYNTETIC WE014 BY TG/DTA AND DSC 163
157.2 Edilamar Pereira Da Silva GALVO. F. P DETERMINATION OF KINETIC PARAMETERS BY OZAWA OF ALKALOID SYNTETIC WE014 164
158 Rachel Ouvinha De Oliveira OLIVEIRA R. DEVELOPMENT OF GOLD NANOPARTICLES CONTAINING DOCETAXEL 165
160 Artur Felipe Costa Amorim AMORIM. A. F. C
EVALUATION IN VITRO OF SCHINUS TEREBINTHIFOLIUS ANTIOXIDANT ACTIVITY BY CAPTURE OF FREE
RADICAL 2.2-DIFENIL-1-PICRILIDRAZIL (DPPH)
166
161.1 Karla Monik Alves Da Silva SILVA. K. M. A. ANTIMICROBIAL ACTIVITY OF ANNONA CORIACEA L. AGAINST ORAL CAVITY BACTERIA 167
161.2 Karla Monik Alves Da Silva SILVA. K. M. A.
MINIMUM INHIBITORY CONCENTRATION OF ACANTHOSPERMUM HISPIDUM DC FRONT OF STREPTOCOCCUS
ORALIS BYTHE MICRODILUTION AND AGAR DIFFUSION METHODS
168
162.1 Laianne Carla Batista Alencar ALENCAR. L.C.B. ANTIMICROBIAL ACTIVITY OF ACANTHOSPERMUM HISPIDUM DC. BY MICRODILUTION 169
162.2 Laianne Carla Batista Alencar ALENCAR. L.C.B.
MICROBIOLOGICAL COMPARISON OF HYPTIS MUTABILIS BRIQ ACTION. BY THE MICRODILUTION AND AGAR
DIFFUSION METHODS
170
163.1
Fernando Jos De Lima
Ramos Jnior
RAMOS JNIOR. F. J. L.
DIAGNOSIS OF HYGIENIC-SANITARY CONDITIONS AND COMPLIANCE WITH GOOD MANUFACTURING
PRACTICES IN FOOD SERVICE AT PUBLIC DAY CARE CENTERS IN CAMPINA GRANDE-PB
171
163.2
Fernando Jos De Lima
Ramos Jnior
RAMOS JNIOR. F. J. L.
PREVALENCE OF INTESTINAL PARASITES AMONG STUDENTS OF PUBLIC DAY CARE CENTER IN THE CITY OF
CAMPINA GRANDE-PB
172
164 Jamilly Kelly Oliveira Neves APOLINRIO. A.C. FORMULATION AND EVALUATION OF SUNSCREEN EMULSIONS USING EXPERIMENTAL DESIGN 173
165.1 Maria Luiza Carneiro Moura MOURA. M.L.C
INTRINSIC DISSOLUTION: THE INFLUENCE OF THE TECHNIQUE ON THE KINETIC PARAMETERS EVALUATION OF
ANTIRETROVIRAL EFAVIRENZ
174
165.2 Maria Luiza Carneiro Moura MOURA. M.L.C
INTRINSIC VERSUS APPARENT DISSOLUTION: INFLUENCE OF COMPRESSION ON THE KINETIC EVALUATION OF
175
167 Joedna Cavalcante Pereira PEREIRA. J.C.
INVESTIGATION OF THE SPASMOLYTIC ACTION OF SOLANUM STRAMONIFOLIUM JACQ. ON RAT AORTA AND
GUINEA-PIG ILEUM
176
168 Vanessa Duarte De Almeida ALMEIDA. V. D. DE KINETIC STUDIES FOR ITRACONAZOLE USING OZAWA MODEL BY DYNAMIC THERMOGRAVIMETRY 177
169
Mariana Anglica Oliveira
Bitencourt
BITENCOURT. MAO
PHYTOCHEMICAL STUDY AND ANTI-INFLAMMATORY ACTIVITY OF AQUEUS EXTRACTS AND FRACIONS OF
MIMOSA TENUIFLORA (JUREMA PRETA) AND HANCORNIA SPECIOSA (MANGABEIRA)
178
170
Jahamunna Abrantes Andrade
Barbosa
BARBOSA. J. A. A. THERMAL ANALYSIS MULTCOMPONET COMPLEX WITH -CD AND METHOTREXATE 179
171
Dayanne Tomaz Casimiro Da
Silva
SILVA. D.T.C. PSEUDO-TERNARY PHASE DIAGRAMS AS A TOOL TO PRODUCE EMULSION E MICROEMULSION 180
172
Rayanne Sales De Arajo
Batista
BATISTA. R. S. A.
DETERMINATION OF MINIMUM INHIBITORY CONCENTRATION OF HYDROALCOHOLIC EXTRACT PLANT ON
CANDIDA SPECIES OF MEDICAL INTEREST
181
174 Adriano Francisco Alves ALVES. A. F THE CURINE ANTIINFLAMMATORY EFFECT IS A CYTOKINE DEPENDENT MECHANISM. 182
175.1 Rayllan Rodrigues RODRIGUES. R. O. DTA/TGA/DTG ANALYSIS OF EUTERPE OLERACEA (AA) GLYCOLIC EXTRACT 183
175.2 Rayllan Rodrigues RODRIGUES. R. O. KINETICS STUDIES OF UVB SUNSCREENS: ETHYLHEXYL TRIAZONE AND ETHYLHEXYL METHOXYCINNAMATE 184
176 Ariane Priscilla M. Tenrio TENRIO. A. P. M
LECTIN ANTINOCICEPTIVE AND ANTI-INFLAMMATORY ACTIVITY EXTRACTION. PURIFICATION AND EVALUATION
OF CANNA LIMBATA ROSCOE SEEDS
185
177.1 Ipojucan Silva Fontes FONTES. I. S.
RHEOLOGICAL ANALYSIS IN PRELIMINARY STABILITY STUDIES OF SUNSCREEN EMULSIONS CONTAINING AAI
EXTRACT (EUTERPE OLERACEAE).
186
177.2 Ipojucan Silva Fontes FONTES. I. S. EVALUATION OF SUNSCREENS THERMAL ANALYSIS BY THERMOANALYTIC TECHNIQUES 187
179 Melina Bezerra Loureiro
NENHUM AUTOR
SUBLINHADO
STUDY OF ASSOCIATION BETWEEN OPG POLYMORPHISMS (- 163A>G; 1181G>C) AND MRNA EXPRESSION IN
TYPE 1 DIABETES PATIENTS
188
180 Denise Porfirio Emerenciano EMERENCIANO. D.P.
THIRD ORDER EXPERIMENTAL APPROACH FOR HUMIDITY DETERMINATION IN THE LEAVES OF AZADIRACHTA
INDICA A. JUSS
189
181 Heglayne Pereira Vital Da Silva SILVA. H.P.V
HUMAN LEUCOCYTE ANTIGEN REGION AND DIABETIC NEPHROPATHY IN CHILDREN AND ADOLESCENTS WITH
TYPE I DIABETES OF RIO GRANDE DO NORTE. BRAZIL
190
182
Layany Carolyny Da Silva
Mouro
NENHUM AUTOR
SUBLINHADO
PREPARATION AND CHARACTERIZATION OF INCLUSION COMPLEXES FORMED OF ESSENTIAL OILS OF LIPPIA
SIDOIDES CHAM AND LIPPIA ORIGANOIDES HBK WITH -CYCLODEXTRIN
191
183 Karla Simone Costa De Souza SOUZA. K.S.C
ASSOCIATION BETWEEN IGF-1 (-1245G>A). TGF-1 (-509C>T) AND LRP5 (126188 C>T) POLYMORPHISMS IN THE
DEVELOPMENT TYPE 1 DIABETES
192
184
Daiane Rafaela Meira Ribeiro
Batista
BATISTA. D.R.M.R
VALIDATION OF UV SPECTROPHOTOMETRIC ANALYTICAL METHOD FOR THE DETERMINATION OF
PRAZIQUANTEL IN INCLUSION COMPLEXES WITH -CYCLODEXTRIN IN PRESENCE OF
HYDROXYPROPYLMETHYLCELLULOSE
193
185 Menilla Maria Alves De Melo MELO. M.M.A.
EVALUATION OF COMPONENTS OF METABOLIC SYNDROME IN ADOLESCENTS WHO ARE OVERWEIGHT
ACCORDING TO THE NATIONAL CHOLESTEROL EDUCATION PROGRAM - ADULT TREATMENT PANEL III
194
186 Jssica De Ftima Vianna VIANNA. J. F
ENERGETIC ASPECTS OF CHEMICAL INTERACTION BETWEEN THE VIOMICYN ANTIBIOTIC AND BACTERIAL
RIBOSOME
195
187 Raquel De Melo Barbosa BARBOSA. R. M
VALIDATION OF A HPLC METHOD FOR THE QUANTIFICATION OF DIBUCAINE IN SOLUTION AND ENCAPSULATED
IN SOLID LIPID NANOPARTICLES OR NANOSTRUCTURED LIPID CARRIERS
196
188.1 Itavielly Layany Frana Feitosa FEITOSA. I. L. F EVALUATION OF ANTIBACTERIAL POTENTIAL OF THE LEAVES OF SENNA OCCIDENTALIS (L.) LINK 197
188.2 Itavielly Layany Frana Feitosa FEITOSA. I. L. F
EVALUATE OF THE ANTIFUNGAL ACTIVITY OF SENNA OCCIDENTALIS (L.) LINK.S LEAVES FORWARD TO
STRAINS OF CANDIDA SPP
198
189 Karla Samara Rocha Soares. SOARES. K.S. R CHITOSAN NANOPARTICLES FOR PROTEIN DELIVERY OBTAINED BY IONIC GELATION 199
190
Melissa Alexandra Da Rosa
Costa
COSTA. M.A.R
CLINICAL-EPIDEMIOLOGICAL PROFILE OF PATIENTS WITH BREAST CANCER IN TO THE COMBINATION CANCER
CHEMOTHERAPY 5-FLUOROURACIL. EPIRUBICIN AND CYCLOPHOSPHAMIDE
200
191.1 Geisa Nobre Oliveira OLIVEIRA. G. N.
ATIMICROBIAL ACTIVITY OF BIOACTIVE METABOLITES ISOLATED FROM STREPTOMYCES SPP. AGAINST
PATOGENIC FUNGI YEAST
201
191.2 Geisa Nobre Oliveira OLIVEIRA. G. N. ACUTE TOXICITY OF CARVACROL 202
192 Liliane De Queirs Souza SOUZA. L.Q.S.
EVALUATION OF THE TOXICITY OF PRADOSIA HUBERI HYDROALCOHOLIC EXTRACT ON THE HYPOPHYSIS-
GONADAL AXIS OF PREGNANT FEMALE RATS
203
193 Deysiane Oliveira Brando BRANDO. D. O.
DRUG PROFILE OF ELDERLY DIABETES AND DYSLIPIDEMIA ATTENDED BY PHARMACEUTICAL CARE PROGRAM
IN A COMMUNITY PHARMACY
204
194 Paulo Ricardo Silva Pereira PEREIRA. PRS EVALUATION OF HEMATOPOIESIS IN MALNOURISHED MOUSE MODEL 205
195 Raquel Arajo De Oliveira OLIVEIRA. R. A REPORTING OF DRUGS INDICATORS OF ADVERSE DRUG REACTIONS: STUDY IN A UNIVERSITY HOSPITAL 206
196
Gustavo Henrique De Medeiros
Oliveira
OLIVEIRA. G.H.M. STUDY OF TAQI/TGF POLYMORPHISM IN NON-SYNDROMIC CLEFT LIP AND/OR PALATE 207
197 Maryanne Melo. MELO. M. N. PHYLLANTUS NIRURI L. REDUCE DAMAGE IN THE MUCOSAL IN MODEL OF COLITIS 208
198 Janaina Da Silva Goes GOES. J. S. COMPATIBILITY BETWEEN CAPTOPRIL AND EXCIPIENTS THROUGH THERMAL ANALYSIS 209
199 Jos Izak Ribeiro De Arajo ARAJO. J.I. R
INTESTINAL PARASITES IN CHILDREN AND FOOD MANIPULATORS OF SCHOOL AND RESIDENTIAL OF A
MUNICIPAL NURSERY IN JOO PESSOA- PB
210
200.1 Evelyn Mirella Lopes Pina PINA. E. M. L
CHARACTERIZATION OF SPRAY-DRIED PRODUCT AND LIOPHILIZED DRIED EXTRACT OF BAUHINIA FORFICATA
LINK BY INFRARED SPECTROSCOPY
211
200.2 Evelyn Mirella Lopes Pina PINA. E. M. L
EVALUATION OF INTERFERING LIPOPHILIC ON DETERMINATION OF CONTENT OF TOTAL FLAVONOIDS BY
SOLID PHASE EXTRACTION
212
201.1 Monize Santos Peixoto PEIXOTO. M.S
PHYSICAL CARACTERIZATION OF SPRAY-DRIED PRODUCT AND LIOPHILIZED DRIED EXTRACT OF BAUHINIA
FORFICATA LINK BY SCANNING ELECTRON MICROSCOPY (SEM) AND X-RAY DIFFRACTION.
213
201.2 Monize Santos Peixoto PEIXOTO. M.S
EVALUATION OF HYGROSCOPIC IN SPRAY-DRIED PRODUCT (SDP) AND LYOPHILISATE DRIED EXTRACT (LDE)
OF LEAVES OF BAUHINIA FORFICATA LINK
214
202 Rafael Jorge Melo De Omena
OMENA. RAFAEL
JORGE MELO
SYNTHESIS. CHARACTERIZATION AND EVALUATION OF THE LEISHMANICIDAL ACTIVITY OF MEGLUMINE
ANTIMONIATE IN PROMASTIGOTE FORMS OF LEISHMANIA MAJOR
215
203 Nataly Miranda Do Nascimento NASCIMENTO. N. M.
INTERACTIONS OF ANTI-HYPERTENSIVE THERAPY DISPENSED BY THE MUNICIPAL HEALTH NETWORK OF
MACEIO / AL
216
204.1 Joo Paulo Tavares Malheiro MALHEIRO. J. P.T
USE OF STATISTICAL PROCESS CONTROL AS A TOOL IN OPTIMIZING THE QUALITY OF THE PRODUCTION OF
DIPYRONE 500 MG/ML ORAL SOLUTION DROPS
217
204.2 Joo Paulo Tavares Malheiro MALHEIRO. J. P.T
ENVIRONMENTAL MONITORING IN THE FILLING LINE DROPS: AN ANALYSIS OF THE
ENVIRONMENT. PRODUCTIN PROCESS AND FINISHED PRODUCT DIPYRONE 500MG
218
205.1 Fabola Llis De Carvalho CARVALHO. F. L. PRELIMINARY EVALUATION OF THE ANTINOCICEPTIVE ACTIVITY OF THE SESQUITERPENE NEROLIDOL 219
205.2 Fabola Llis De Carvalho CARVALHO. F. L. SEROLOGICAL STUDY OF CHAGAS DISEASE IN THE CITY OF SALGADINHO/PB 220
206 Alan Leite Moreira. MOREIRA. A.L.
EVALUATION OF PHYSICAL AND CHEMICAL COMPOSITION OF THREE CULTIVARS OF PAPAYA SEEDS
PRODUCED IN THE NORTHEAST OF BRAZIL FOR POSSIBLE RECOVERY IN FOOD INDUST
221
207.1 Sarah Rafaelly Arajo Santos SANTOS. S.R.A. EXTRACTION AND CHARACTERIZATION OF CHITIN FROM SHRIMP WASTE OF RIO GRANDE DO NORTE - BRAZIL 222
207.2 Sarah Rafaelly Arajo Santos SANTOS. S.R.A. EVALUATION OF THE AUTHENTICITY OF A SAMPLE OF SENNA MARKETED IN NATAL-RN. 223
208.1 Matheus Anglos ANGELOS. M.A.
EVALUATION OF QUALITY OF SAMPLES OF BACCHARIS TRIMERA MARKETED IN DIFFERENT CAPITALS OF THE
BRAZILIAN NORTHEAST THROUGH HPLC ANALYSIS
224
208.2 Matheus Anglos ANGELOS. M.A. ANALYSIS OF EXTENDED RELEASE TABLETS BENZNIDAZOLE BY RAMAN SPECTROSCOPY AND MICROSCOPY 225
209.1 Fabiana Paiva Galvo GALVO. F. P DETERMINATION OF KINETIC PARAMETERS BY OZAWA FOR ALKALOID SYNTETIC WE010 226
209.2 Fabiana Paiva Galvo GALVO. F. P THERMAL CHARACTERIZATION OF ALKALOID SYNTETIC WE010 BY TG/DTA AND DSC 227
210 Monique Dielly JANURIO. M.D.A.
PARTICIPATION IN CLINICAL PAIN. FOLLOWING THE TREATMENT OF ANTIINFLAMMATORY NON-STEROIDAL
ONCOLOGY WARD ASSISTANCE FOUDATION OF PARAIBA FAP
228
211
Elton Max Nascimento Do
Egito
EGITO. E. M. N. THE QUEST FOR ION CHANNEL MEMORY USING A LIPID MEMBRANE 229
213 Tamires Andrade Da Silva
NENHUM AUTOR
SUBLINHADO
PREPARATION OF DAPSONE-SILICATE NANOCOMPOSITES 230
214 Raul Hernandes Bortolin BORTOLIN. R.H.
INFLUENCE OF HIGH GLUCOSE CONCENTRATION ON BONE METABOLISM IN MICE C57BL/6 INDUCED WITH
HIGH-FAT DIET
231
215.1 Dayane Beserra Costa COSTA. D.B. CLINICAL AND EPIDEMIOLOGICAL ANALYSIS OF SCORPION STINGS IN CAMPINA GRANDE: PART II 232
215.2 Dayane Beserra Costa COSTA. D.B. SCORPIONIC AND OPHIDIAN ACCIDENT EPIDEMIOLOGIC PROFILE NOTIFIED IN CAMPINA GRANDE CITY 233
216 Mrian Letcia Carmo Bastos BASTOS. M. L. C HYPERTENSION: EDUCATIONAL ACTIVITY IN PUBLIC HEALTH 234
218.1
Leslie Raphael De Moura
Ferraz
FERRAZ. L.R.M. ANALYSIS OF DIFFERENT BATCHES OF BENZIDAZOLE BY HPLC AND FTIR 235
218.2
Leslie Raphael De Moura
Ferraz
FERRAZ. L.R.M.
UTILIZAO DE TCNICAS ESPECTROMTRICAS PARA A CARACTERIZAO DO 3-(4-CLORO-BENZIL)-5-(4-
NITRO-BENZILIDENO)-IMIDAZOLIDINA-2.4-DIONA (LFPS/FZ4)
236
220 Mizia Martins COSTA. M. K. C. M.
IDENTIFICAO E SUSCETIBILIDADE ANTIMICROBIANA DE ENTEROCOCOS ISOLADOS NA CIDADE DO
NATAL/RN. BRASIL
237
222
Daliana Maria Berenice De
Oliveira Souza
SOUZA. DMBO
HUMAN LEUKOCYTE ANTIGEN-G AND -E EXPRESSION IN GASTRIC SPECIMENS IN PATIENTS WITH
HELICOBACTER PYLORI
238
223 Joanda Paolla SILVA. J. P. R. COST-EFFECTIVENESS OF ANTIHYPERTENSIVE THERAPIES IN BASIC HEALTH SYSTEM 239
224.1 Wende Soares FREITAS. W.S.
SURVEY OF RISK FACTORS FOR PREVALENCE OF CASES OF TYPE 2 DIABETES MELITTA IN THE CITY OF
ARARA-PB
240
224.2 Wende Soares FREITAS. W.S. EPIDEMIOLOGICAL SURVEY IN CASES OF HEPATITIS TOWN OF CUITE - PB. BRAZIL. 2011. 241
225 Gubbio Matos Da Silva SILVA. G. M. S
USE OF BABASSU IN THE ADSORPTION OF CHEMICALS FOR WASTE TREATMENT IN INDUSTRY CHEMICAL AND
PHARMACEUTICAL
242
226 Lidyane Neves Miranda MIRANDA. L.N HUMAN LEUKOCYTE ANTIGEN-G AND TH17 EXPRESSION IN CERVICAL INTRAEPITHELIAL NEOPLASIA 243
227.1
Antnia Rosngela Soares
Penha
PENHA. A. R. S1 POSSIBLE ANTINOCICEPTIVE EFFECT OF LIPPIA MICROPHYLLA (VERBENACEAE) ESSENTIAL OIL IN MICE 244
227.2
Antnia Rosngela Soares
Penha
PENHA. A. R. S1
INVESTIGATION OF ACUTE TOXICITY AND MIORRELAXANT EFFECT OF LIPPIA MICROPHYLLA (VERBENACEAE)
ESSENTIAL OIL IN MICE
245
228
Cinthya Maria Pereira De
Souza
SOUZA. C.M.P.
CHARACTERIZATION OF THE OPUNTIA FICUS-INDICA (L.) MILL DRIED EXTRACT BY THERMAL ANALYSIS AND
INFRARED SPECTROSCOPY
246
229 Cllio Diogo Soares
NENHUM AUTOR
SUBLINHADO
EXPRESSION STUDY OF THE GENES INVOLVED ON FOLIC ACID METABOLISM IN ASSOCIATION WITH ORAL
CLEFT DEVOLOPMENT
247
230 Flvia Cmara De Souza SOUZA. F. C PARAMETERS KINETICS BY OZAWA MODEL FOR NICOTINAMIDE BY DYNAMIC TG 248
231 Arsnio Rodrigues Oliveira OLIVEIRA. A.R
DEVELOPMENT AND VALIDATION OF A STABILITY INDICATIVE METHOD OF ASCORBIC ACID (VIT C) FOR 500 MG
TABLETS
249
232 Laura Da Fonseca Ferreira FERREIRA. L.F
DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR QUANTIFICATION OF SIMVASTATIN IN
MICROEMULSION BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
250
232.2 Arsnio Rodrigues Oliveira OLIVEIRA. A.R
RETROSPECTIVE AND CONCURRENT VALIDATION OF THE MANUFACTURING PROCESS OF ZIDOVUDINE 300MG
+ LAMIVUDINE 150MG
251
233 Regina Carmen Espsito ESPSITO. R.C.
A STUDY OF CYTOTOXICITY BY BIOASSAY TECHNIQUES OF THE HOMEOPATHIC PRODUCTS CONTAINING
MOMORDICA CHARANTIA
252
234 Thaciane Da Cunha Soares SOARES. T.C UV-VIS ANALYSIS OF CHEMICAL MARKERS OF THE LEAVES OF PASSIFLORA EDULIS SIMS VARIETY EDULIS 253
236
Pedro Henrique Dos Santos
Soares
SOARES. P.H.S.
ANTICONVULSIVANT AND ANXIOLITIC ACTION EVALUATION OF INDIGOFERA SUFFRUTICOSA MILLS AND
INDIGO CARMINE IN MICE
254
237 Lais Farias Azevedo AZEVEDO. L. F
DEVELOPMENT OF NEW METHODS OF ANALYSIS AND CONTROL OF
METRONIDAZOLE TABLETS USING NEAR INFRARED
255
238.1 Marcelo Amorim AMORIM. M.V.P. CARACTERIZATION OF RIFAMPICIN AND THEIR DEGRADATION PRODUCTS BY CHEMICAL STRESS 256

238.2 Marcelo Amorim AMORIM. M.V.P. COMPARISON STUDY OF TWO ANALYTICALS METHODS OF 4-FDC USING A SINGLE COLUMN 257
239
Leandro Vinicius Fernandes De
Morais
MORAIS. L.
EVALUATION OF ANALYTICAL METHODS APPLIED TO QUANTIFICATION OF LIPID PEROXIDATION IN LIVER
HOMOGENATE OF RATS
258
240 Gabriela Diniz Fonseca FONSECA. G. D.
VALIDATION OF AN UV SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF PROPRANOLOL IN
CAPSULES USING QUITOSAN AS EXCIPIENT
259
241 Julia Morais Fernandes FERNANDES. J. M.
CHROMATOGRAPHIC PROFILE OF THE CRUDE EXTRACT AND FRACTIONS FROM K. BRASILIENSIS CAMBESS
(SAIO) LEAVES
260
242.1 Cludia Cecilio Daher DAHER. C. C. KINETCS STUDIES OF EUTERPE OLERACEA (AA) EXTRACTS 261
242.2 Cludia Cecilio Daher DAHER. C. C. SUNSCREENS O/W EMULSIONS STABILIZED BY RHEOLOGICAL MODIFIERS: PRELIMINARY STABILITY TESTS 262
243 Jos Xavier De Lima Neto LIMA NETO. J. X.
STUDY BY QUANTUM MECHANICAL OF INTERACTIONS BETWEEN AMPA RECEPTORS AND FLUORINE
WILLARDIINE
263
244 Vanessa Santos Temponi TEMPONI. VS EVALUATION OF THE ANTI-INFLAMMATORY POTENTIAL ETHANOLIC EXTRACT FROM VERNONIA POLYANTHES 264
245 Jucelia Barbosa Da Silva. SILVA. J.B EVALUATION OF THE ANTI-INFLAMMATORY POTENTIAL ETHANOLIC EXTRACT FROM VERNONIA CONDENSATA 265
246 Vanda Lucia dos Santos
SANTOS, V.L
EVALUATION OF MUTAGENICITY AND ACUTE TOXICITY IN MICE OF Spondias purpurea (Siriguela) ETHANOLIC
EXTRACT.
266
Challenge and opportunities for development new diagnose methods and pharmaceutical products

1

CODE 1

IMMUNOPHENOTYPIC AND FUNCTIONAL CHARACTERIZATION OF
HEMATOPOIETIC STEM CELLS AFTER MOBILIZATION IN MALNOURISHED
MICE

Pereira, P. R. S.
1
; Silva, B. C. O.
1
; Avelino, C. U. R.
1
; Cavalcanti Junior, G. B.
2
;
Borelli, P.
3
; Schimieguel, D. M.
1

1
Laboratrio de Hematologia Clnica; Departamento de Anlises Clnicas e
Toxicolgicas/CCS/UFRN.
2
Laboratrio de Imunologia Clnica; Departamento de Anlises Clnicas e
Toxicolgicas/CCS/UFRN.
3
Faculdade de Cincias Farmacuticas/ Departamento de Anlises Clnicas e
Toxicolgicas/USP.
Corresponding author: pauloricardo306@yahoo.com.br

Introduction: Hematopoietic stem cell transplantation (HSCT) is a therapy
performed in persons with hematologic malignancies. Recruitment of stem cells
from the bone marrow (BM) into peripheral blood (PB) is termed mobilization. Bone
marrow hypoplasia has generally treated intensively may decrease the
effectiveness of therapy, similar to that found in malnutrition. Objectives: To assess
the functional status and immunophenotypic profile of hematopoietic stem cells
after mobilization in a murine model of malnutrition and bone marrow hypoplasia.
Materials and Methods: We studied eight adult male BALB/c separated in two
groups: malnourished (D) and control (C). The group D (n = 5) received low-
protein diets for five weeks, and group C (n = 3) received normal protein diets ad
libitum. All animals were mobilized with G-CSF fo 5 dys dose 100g/Kg/dy.
On sixth day, animals were euthanized to obtain PB and BM, by washing the
medullary cavities of femurs and tibias. BM samples were cultured in
methylcellulose plus growth factors (IL-3, G-CSF, GM-CSF) for functional
evaluation by counting the colony-forming units (CFU); G-CFU and GM-CFU.
Quantification of HSC was performed by immunophenotyping (CD34
+
and Sca-1
+
).
Results: There was no significant difference in quantification of HSC present in
BM/PB between the two groups. Total CFU and CFU-G were significantly
decreased in samples of group D than in group C (p = 0, 0357). Conclusion:
There was no difference in amount of cells mobilized from the BM niche of
malnourished group. However, functionally the HSC seem somehow damaged,
with reduced differentiation and proliferation.

Financial support from UFRN.


2

CODE 2
IN VITRO ACARICIDAL AND CYTOTOXIC ACTIVITY OF AQUEOUS
EXTRACTS OF Agave sisalana PERRINE AND Furcraea selloa K.
KOCH

Macedo, A. L.
1
; Prata, M. C. A.
2
; Chedier, L. M.
3
; Sousa, O. V.
4
; Pimenta, D.
S.
3
; Pires, M. F. A.
2
.

1
Phmceuc scences mses suden, UFJF.
2
Embrapa Dairy Cattle researchers.
3
Botanical Department, Biologic Sciences Institute, UFJF.
4
Pharmaceutic Department, Pharmacy College, UFJF.
Corresponding author: thumcd@gmail.com

Introduction: Agave sisalana Perrine e Furcraea selloa K. Koch, part of
Agvcee fmy nd popuy known s p, hve been used s
pesticides and antimicrobials agents in ethnoveterinary, especially as
acaridal. Objective: Evaluate the acaricial and cytotoxic activities of the
leaves infused of A. sisalana and F. selloa. Material and Methods: F.
selloa and A. sisalana leaves were collected in Juiz de Fora, Minas Gerais,
Brazil. The aqueous extract was obtained by infusion of the dried and
crushed leafs. Acaricidal activity was evaluated against Rhipicephalus
(Boophilus) microplus at extracts using the extracts in concentration ranging
of 40mg/mL to 200mg/mL. The citotoxic activity was tested against Artemia
salina exposed to extracts in concentrations ranging of 10 to 1000 g/mL.
Results and Discussion: Extracts don nefeed sgnfcny n yng
and hatching, but A. sisalana extract showed a light increase in the laying
and the F. selloa extract showed a light decrease of the same. The test
population showed resistance to pyrethroids, organophosphates and
mdnes sngy nd n ssocon. The excs ween oxcs gns A.
salina in the tested concentrations, having F. selloa killed 22,5% and A.
sisalana 10,0% of the nauplii in the higher concentration. These results
corroborate with previous publication were the acaricidal activity were
associated with the cytotoxicity probably linked with the saponins presents
in these species. Conclusion: The obtained results indicate that the leaves
nfused of boh speces don hve ccd nd cooxc cves. Nex
studies should focus on the leaves methanolic extracts of F. selloa.

Financial support from FAPEMIG and master scholarship from
Embrapa dairy cattle.


3

CODE 3
PRELIMARY STUDY OF BORNEOL SALICILATE ANTIINFLAMATORY
ACTIVITY

Vasconcelos, R. M. C.; Leite, J. A.; Leite, F. C.; Rodrigues, L. C.;
Piuvezam, M. R.

Laboratrio de Imunofarmacologia, Centro de Biotecnologia, Universidade
Federal da Paraba (UFPB).
Laboratrio de Farmacoqumica, Centro de Biotecnologia, Universidade
Corresponding author: renatacostav@gmail.com

Introduction: Borneol Salicilate (BoS) is a synthetic compound obtained
by esterification reaction of salicylic acid and (-)-borneol alcohol. The
diastereomer (-)-borneol has been described as a potential anti-
inflammatory molecule in neuronal cells. Objective: The goal of this study
was to evaluate BoS in murine models of inflammation. Material and
Methods: The experimental models used were carragenaan-induced paw
edema and zymosan-induced peritonitis. Swiss mice (n=6) orally
pretreated with 50, 100 or 200 mg/kg BoS or 200 mg/kg acetylsalicylic acid
(AAS) as control drug receved n njecon of 20 L of cgenn
(2,5%) in left paw and NaCl 0,9% solution in right paw. The paw volume
was measured in different times to evaluate the edema formation. In the
peritonitis experimental model, pretreated animals (n=8) received an
npeone njecon of 500 L of zymosn (2 mg/mL) nd 4 hous fe
the peritoneum was exposed and the fluid was collected with 1 mL ice-cold
PBS. The aspirate was centrifuged and the cells were ressuspended and
counted in hemocitometer. Results and Discussion: The pretreatment
with BoS (200 mg/kg) was significantly (p<0.05) effective in inhibiting the
paw edema in 1h, 2h, 6h and 24h, as well as the AAS; however, the minor
doses of BoS were not effective. The BoS (200 mg/kg) also reduced
significantly (p<0.05) the neutrophil migration to peritoneum. Conclusion:
These preliminary data suggest that BoS has anti-inflammatory effects
related with eicosanoid mediators.

Financial support from CNPq/Brazil and master scholarship from
CAPES/Brazil


4

CODE 4
ESTIMATION OF CARDIOVASCULAR RISK BY THE FRAMINGHAM
SCORE IN HYPERTENSIVE PATIENTS

Portela, A. S.
1,2
; Silva, P. C. D.
2
; Falco, R. A.
2
; Malheiro, J. P. T.
2
;
Montenegro Neto, A. N.
2
; Almeida, M. G.
1,3
; Simes, M. O.
2

1
Programa de Desenvolvimento e Inovao Tecnolgica de
Medicamentos, Universidade Federal do Rio Grande do Norte (UFRN).
2
Laboratrio de Pesquisa em Bioqumica Clnica, Departamento de
Farmcia, Universidade Estadual da Paraba (UEPB).
3
Laboratrio Multidisciplinar, Departamento de Anlises Clnicas e
Toxicolgicas, UFRN.
Corresponding author: moscg@uol.com.br

Introduction: Cardiovascular diseases are the leading cause of death in
Brazil. The main risk factors for these diseases are Arterial
Systemic Hypertension, dyslipidemia, smoking and Diabetes
Mellitus (DM). Material and Methods: Based on this framework, this
study aims to evaluate the cardiovascular risk of hypertensive
patients by the Framingham risk score. It is a cross-sectional study which
included a random sample of 53 individuals with hypertension treated at
the Municipal Health Service of Campina Grande, in Paraba. It was
adopted the Framingham score, taking into account the sum
of points obtained from the set of risks, from the age, presence of
diabetes, the practice of smoking, blood pressure and the ratio
between total cholesterol and HDL. Results and Discussion: From the
results, it was observed that 81.25% of the male population had risk equal
or more than 10% for cardiovascular complications estimated at 10
years, according to Framingham scores. In women, on the contrary,
statistical analysis showed that this risk was 76.47% less than or equal to
8%. Conclusion: The results showed the importance of implementing
the score in Primary Care, which allow for early diagnosis of people at risk
and identify remedial measures that may be effective in treating
and preventing cardiovascular events, many of them fatal, avoiding
personal suffering and reducing costs to the health system and society.

Financial support: Incentive Program for Graduate Studies and
Research (PROPESQ) UEPB


5

CODE 5
CHARACTERIZATION OF A CATIONIC NANOEMULSION INTENDED
TO GENE THERAPY AFTER STERILIZATION PROCESSES

Silva, A. L.
,
; Alexandrino-Junior, F.
,
; Silva, K. S.; Verssimo, L. M.
,
;
Lima, L. F. A.; Egito, E. S. T.

Laboratrio de Sistemas Dispersos (LaSiD), Departamento de Farmcia
(UFRN);
Laboratrio de Biologia Molecular e Gentica (LBMG), Centro de
Biocincias (UFRN).
Corresponding author: socrates@ufrnet.br

Introduction: Gene therapy is based on the transfer of genetic material
into the target defective tissue or cell. For its success, it is necessary to
develop carriers able to bypass both, membrane and citosol barriers,
reaching the nucleus. Most of the researchers deals with viral approaches.
However, concerns about safety have increased studies on non viral gene
delivery systems like nanoemulsions. Objective: The aim of this work was
to evaluate autoclaving and filtration as methods to guarantee a cationic
nanoemulsion system sterility, which makes in vitro assays possible.
Material and Methods: The nanoemulsion, prepared by sonication
method, was made of Captex 355, Span 80, Tween 80, water and
stearylamine. Sterilization processes was done as follow: filtration by 0.22
m filter and autoclaving for 15 min at 1 atm and 121C. Then,
characterization by macroscopic analyses, zeta potential and size
measurements were performed. Results and Discussion: The results
showed that, as expected, phase separation happens when autoclaving
was used to sterilize the nanoemulsion (a thermodynamically unstable
system). Concerning the filtration method, the characteristics of the system
were maintained. The unsterilized nanoemulsion presented size of 240 nm
and zeta potential value of +26.6 mV. After filtration, the system presented
233 nm of size and +23.9 mV of Zeta Potential. Polidispersivity Index
decreased from 0.201 to 0.176 after filtration, meaning that size distribution
was more homogenous after the filtration process. Conclusion: These
results demonstrate that sterile filtration is a suitable process to sterilize
cationic nanoemulsion for gene therapy making possible the future use of
cationic nanoemulsions for gene delivery.


6

CODE 6
SV1: ONE POLYSACCHARIDE POPULATION FROM Sargassum
vulgare WITH ANTICOAGULANT AND ANTITHROMBOTIC ACTIONS
AND THROMBIN INHIBITOR

Dore, C. M. P. G.
1
; Alves, M. G. C. F.
1
; Medeiros, H. N.
1
; Paiva, A. A. O.
1
;
Filgueira, L. G. A.
1
; Leite, E. L.
1

1
Departamento de Bioqumica, Universidade Federal do Rio Grande do
Norte (UFRN).

Introduction: The activation of the blood coagulation cascade results in
the formation of the factor Xa which consequently catalyzes the formation
of thrombin. Thrombin converts fibrinogen to fibrin, which polymerizes to
form a clot. Sulfated fucans (fucoidans) were isolated of species from
brown seaweed showing anticoagulant activity. Objective: In this study
was evaluated the anticoagulant action of fucans extracted from
Phaeophyceae, Sargassum vulgare, investigating its possible course of
action on blood anticoagulation. Material and Methods:
The algae S.
vulgare was collected on the southern coast of Rio Grande do Norte, washed,
processed,delipided and isolated with increasing concentrations of acetone,
obtaining a sulfated polysaccharide called F1.0 fraction. This compound was
chemically characterized and
its effect on the extrinsic and intrinsic
coagulation tests was performed by the thrombin time (PT) and activated
partial thromboplastin time (aPTT).
In addition, was
analyzed the direct
effect of polysaccharides on the enzymatic assay of thrombin and factor Xa
on its chromogenic substrates, as well as in vivo antithrombotic action of
these compounds. Results and Discussion: Evaluation of PT and aPTT
tests showed that the polysaccharides have action on the intrinsic
coagulation pathway. The enzyme inhibition assays showed maximum
inhibition of thrombin in the order of 50.2% and not demonstrated direct
inhibition on factor Xa at 25 g of polysaccharides. The F1.0 fraction
shows strong antithrombotic action with 83.3% inhibition of thrombus
formation in vivo. Conclusion: The results suggest that fraction F1.0 has
a direct effect on thrombin as evidenced by its strong anticoagulant and
antithrombotic actions.


7

CODE 7
ANTI-INFLAMMATORY AND ANTINOCICEPTIVE ACTIVITIES OF
Phyllanthus niruri L. SPRAY-DRIED EXTRACT

Porto, C. R. C.
1
; Soares, L. A. L.
3
; Ferreira, A. A. A.
2
; Souza, T. P.
4
;
Petrovick, P. R.
5
; Lyra, I. L.
6
; Gavioli, E. C.
2
; Guerra, G. C. B.
2
.

1
Programa de Ps-graduao em Cincias Farmacuticas, Universidade
Federal do Rio Grande do Norte.
2
Departamento de Biofsica e Farmacologia, Universidade Federal do Rio
Grande do Norte.
3
Departamento de Cincias Farmacuticas, Universidade Federal de
Pernambuco.
4
Faculdade de Cincias Farmacuticas, Universidade Federal do
Amazonas.
5
Programa de Ps-graduao em Cincias Farmacuticas, Faculdade de
Farmcia, Universidade Federal do Rio Grande do Sul.
6
Iniciao cientfica, Departamento de Biofsica e Farmacologia,
Universidade Federal do Rio Grande do Norte.
Corresponding author: raqquel_c@yahoo.com.br

Introduction: Phyllanthus niruri L., a species long used by peoples of
tropical and subtropical regions. A large number of preclinical studies have
not only confirmed, but also expanded the medicinal uses of species of the
Phyllanthus genus mentioned in traditional medicine. Objective:
investigate possible anti-inflammatory and antinociceptive effects of
Phyllanthus niruri spray-dried extract (PnSDE). Materials and Methods:
Two models were used to evaluate anti-inflammatory properties of PnSDE:
carrageenan-induced paw edema and thioglycolate-induced leukocyte
migration inhibition. Three animal models were use to evaluate
antinociceptive effects: Randall Selitto, tail flick and hot plate. Results and
Discussion: In carrageenan-induced paw edema, PnSDE (100, 200, 800,
and 1600 mg/kg i.p.), evoked considerable edema reduction, suggesting
that PnSDE inhibits different chemical inflammation mediators. It was
observed that leukocyte migration to the peritoneal cavity was greatly
reduced with 100 and 400 mg/kg doses of extract. In the Randall and
Selitto test, PnSDE (100 mg/kg and 200 mg/kg i.p.), showed an important
peripheral analgesic activity with increased nociceptor threshold to painful
stimuli. PnSDE (100 and 200 mg/kg i.p.) in the tail flick and hot plate
methods showed significant central analgesic activity, as evidenced by an
increase in response time to the thermal stimulus. Conclusions: The
PnSDE showed anti-inflammatory properties in both models: leukocyte
migration inhibition and paw edema. PnSDE was also able to reduce
vascular response in the inflammatory process and inhibit leukocyte
migration to the inflammatory site. It can be concluded that PnSDE has
antinociceptive effects in antinociceptive models at both peripheral and
central levels.

Financial support from CNPq/Brazil


8

CODE 8
DETERMINATION OF RALOXIFENE HYDROCHLORIDE USING
ULTRAVIOLET SPECTROPHOTOMETRY AND LC STABILITY-
INDICATING METHOD

Salazar, F. R.
1,*
; Bergold, A. M.
1

1
Laboratrio de Qumica Farmacutica, Faculdade de Farmcia, UFRGS.
Corresponding author: nandi_salazar@yahoo.com.br

Introduction: Raloxifene (RLX) is a selective oestrogen receptor
modulator approved in the USA for the prevention of postmenopausal
osteoporosis. Due to its relevance and the interesting of INCT- IF to
synthesize this molecule, it becomes important to establish methodologies
to assure the quality of the pharmaceutical formulation. Objective: The aim
of the present work was to develop and validate methods by ultraviolet
spectrophotometry (UV) and liquid chromatography (LC) for the
determination of RLX in tablets. Materials and Methods: Samples of
Evista were furnished by INCT-IF and reference chemical substance was
purchased in the local commerce. The UV method was performed with
methanol as solvent and detection at 284nm. The LC analysis used a NST
C18 reverse phase 250x4.6mm column, with mobile phase
water:acetonitrile:trietilamine (67:33:0.3) pH 3.5 running at flow-rate of
1.0ml/min with temperature of 30C and detection at 287nm. The
methodologies were validated and comparative study was performed.
Results and Discussion: Both methods were validated according to ICH
guidelines acceptance. The UV method presented linearity in the range of
3.0 12.0g/ml (r=0.9994). Also it presented specificity, precision and
accuracy. The LC analysis presented linearity in the range of 4.0-16.0g/ml
(r=0.992). Precision, accuracy and no interference from the degradation
products were observed. These methods were compared by statistical
nyss (Sudens es) nd pesened no sgnfcn dffeences beween
them. Conclusions: The proposed methods were successfully applied for
the quantitative determination of RLX. They were statistically equivalent (t-
test), assuring the quality of the pharmaceutical dosage form.

Financial support from INCT_IF/Brazil and master scholarship from
CNPQ/Brazil.


9

CODE 10
AQUEOUS EXTRACT OF Mikania lindleyana DC. PRESENTS
POTENTIAL ANTI-INFLAMMATORY EFFECT

Silva, A. S. B.
1
; Pinheiro, B. G.
1
; Figueiredo, J. G.
3
; Souza, G. E. P.
2
;
Cunha, F. Q.
3
; Lahlou, S.
4
; Barbosa, W. L. R.
1
; Sousa, P. J. C.
1

1
Programa de Ps-Graduao em Cincias Farmacuticas, Universidade
Federal do Par, Belm, Par, Brazil.
2
Laboratrio de Farmacologia, Faculdade de Cincias Farmacuticas,
Universidade de So Paulo, Ribeiro Preto, So Paulo, Brazil.
3
Departamento de Farmacologia, Faculdade de Medicina, Universidade de
So Paulo, Ribeiro Peto, So Paulo, Brazil.
4
Instituto Superior de Cincias Biomdicas, Universidade Estadual do
Cear, Fortaleza, Cear, Brazil.

Corresponding author: andressabriggida@gmail.com

Introduction: Mikania lindleyana DC. (Asteraceae), popularly named
sucuriju, is a common plant in Brazilian Amazonia. Ethnopharmacological
studies show a diversity of uses of this species such in treatment of
gastritis, infection, pain and inflammation. Objective: Investigate the
effects of aqueous extract of M. lindleyana (AEML) on several models of
inflammation in rodents. Materials and Methods: The anti-inflammatory
activity was evaluated using the acetic acid in mice, carrageenan-induced
paw edema in rats as well as cell migration, rolling and adhesion of
leukocytes in carrageenan-induced peritonitis. Results and Discussion:
The AEML, at doses 125, 250, 500, 750, 1000 and 1500 mg/kg,
significantly inhibited (in a dose-dependant way) the number of contortions
induced by acetic acid on writhing test. The median effective dose (ED
50
) of
692.6 mg/kg was able to inhibit the erythema formation when compared to
control group. The AEML inhibited the paw edema carrageenan-induced
from 2
nd
hour and reduced neutrophil migration to peritoneal cavity.
Conclusions: Our results indicate that AEML demonstrate an anti-
inflammatory activity through inhibition of prodution of inflammatory
mediators as PGs, and also on adhesion molecules with involvement of
cytokines.

Financial support from FAPESP/Brazil and CAPES/Brazil


10

CODE 12
DEVELOPMENT AND VALIDATION OF DOXYCYCLINE HYCLATE BY
HPLC METHOD

Kogawa, A. C.
1
; Salgado, H. R. N.
1

1
Laboratrio de Controle de Qualidade, Departamento de Frmacos e
Medcmenos, Unvesdde Esdu Pus Jo de Mesqu Fho
(UNESP), Araraquara, So Paulo, Brazil
Corresponding author: ac_kogawa@yahoo.com.br

Introduction: Doxycycline hyclate (DOX), a broad-spectrum antibiotic, is
part of the Unified Health System (SUS) in Brazil. The HPLC methods
described in the USP (2008), British (2010) and Portuguese (2005)
Pharmacopoeia are time-consuming. Objective: A simple, sensitive and
accurate HPLC method was developed and validated for the assay of DOX
in raw material and tablets. Material and Methods: The method was
developed in a HPLC Waters composed of pump chromatographic
Waters 1525, manual injector Rheodyne Breeze 7725i and UV-Vis
detector Waters 2487, Luna CN column (250 mm x 4.6 mm), mobile
phase water + 0.1% TFA : ACN + 0.1% TFA (60:40, v/v), HCl 0.01M
solutions of DOX and wavelength at 360 nm. Results and Discussion:
The linearity of the method was proven by the components of parallelism,
correlation coefficient, 0.9999, and variance analysis of the results of the
analytical curve. The values obtained for the test intraday and interday
precision were 1.08 and 0.52%, respectively. The amount of DOX in the
samples analyzed, 110.07%, is not consistent with the official compendium
that is 95.0 to 105.0%. The accuracy of the method was proven by the
recovery test, with an estimated average of 100.18%. The robustness was
confirmed by measuring values at wavelength, proportion of mobile phase,
injection volume, flow rate and ACN. Conclusion: The developed HPLC
method was considered suitable for quantification of doxycycline hyclate
tablets. Regarding chromatographic methods described in the literature,
this method demands less time to prepare the mobile phase and has
running time of approximately 5 minutes.

Financial support: CAPES, CNPq, FAPESP, UNESP, Unio Qumica
Pharmaceutical Industry.


11

CODE 13
STRATEGY FOR TREATMENT OF WATER, FEDERAL UNIVERSITY OF
CAMPINA GRANDE / CENTER FOR EDUCATION AND HEALTH-PB

Pinheiro, A. A. V.; Lima, W. S., Arajo, K. A. M.; Alencar, J. S.

Farmcia Escola Manoel Casado de Almeida, Centro de Educao e
Sade, Universidade Federal de Campina Grande (UFCG).
Corresponding author: anderson_avp@hotmail.com

Introduction: The UFCG/CES-PB offers several courses in education and
health. Given the expansion of activities within the CES as the
implementation of the Pharmacy College (PC), the University Restaurant,
research, among others, it came the need to design a Water Treatment
Center (WTC), because the current situation of water is of poor quality for
the projected activities. Objective: Therefore, the objective of this work
includes the development of the floor plan of the WTC, Standard Operating
Procedure (SOP) of the equipment and distribution logistics. The proposed
equipment is designed to work during 4h/day 5days/week, providing
drinking and purified water according to the law. Material and Methods:
The logistics of distribution is designed as follows: water coming from a
natural source with a flow rate of 3.000L/h and 2.22mS of conductivity will
supply a water tank of 5.000L. This water will be treated by three 5m pre-
filters to remove solids, with a desalination capacity of 99% and three
reverse osmosis membranes, acquiring at this stage purified water. This
water will go to a 3.000L reservoir and to a 500L conical bottom for the PC,
obtaining purified water. Thus, the conical reservoir is coupled to an
ultraviolet system, flow of 0.23m
3
/h, 6w-220volts bacterial protection lamp,
and an deionizer of 100L/h with a high degree of chemical purity, obtaining
water with a maximum of 1,3S/cm(25 C) of conductivity. The monitoring
and maintenance of the WTC shall obey the SOPs developed by students
of the PC, which will be routinely revised for answering better the needs of
the UFCG/CES.


12

CODE 14
ADVERSE EVENTS IDENTIFIED IN A HOSPITAL OF THE SENTINEL
NETWORK

Pereira, A. M. S.
1
; Silva, M. L. M.
1
; Porto, M. L. S.
2
; Farias, A. D.
3

1
Academic of Pharmacy, Centro de Educao e Sade, Universidade
Federal de Campina Grande (UFCG).
2
Msc Pharmaceutical Biochemist, University Hospital Alcides Carneiro,
Campina Grande (UFCG).
3
Professor of Pharmacy, Centro de Educao e Sade, Universidade
Federal de Campina Grande (UFCG).
Corresponding author: aurea_marcela@hotmail.com

Introduction: The University Hospital Alcides Carneiro is part of the
Sentinel Network of Hospitals, which aims to do the surveillance in health
products after they are sold, through the investigation of adverse events in
the areas of technical surveillance, pharmacovigilance and
haemovigilance. Objective: To identify and analyze adverse events
occurred in September-2010/September2011 in that hospital. Material and
Methods: It was conducted a retrospective study, through validated
questionnaires of active finding from Anvisa, recorded in the period.
Results and discussion: We recorded 15.0% of adverse events in
haemovigilance, 6.0% of adverse reactions, 38.0% of technical complaints
in pharmacovigilance and 41.0% in technical surveillance. In the last one,
the largest number of adverse events occurred due to the lack of quality in
syringes (63.7%), equipment (12.0%) and urine collection (7.1%). In
pharmacovigilance, the parenteral use of isotonic solutions contributed to
the higher frequency of events (69.9%). Regarding adverse reactions to
medications, dipyrone and ciprofloxacin caused itching and erythematous
dermatitis was completed as causal. Hypertension and cyanosis (2.5%)
was the most significant reaction to the haemovigilance. It was noted that
the events of technical complaints are related to the lack of technical
quality in the fabrication of the product and that the most frequently
observed adverse reactions were allergic. Conclusions: The reported
events bring negative economic consequences for the institution and risks
for the patient, requiring a greater involvement of professionals so that can
be established a continuous monitoring and thus improve the quality of the
treatment with safety to the patient.


13

CODE 15
STANDARDIZATION OF TECHNIQUES FOR EVALUATION OF
HEMATOPOIESIS IN MALNOURISHED MICE

Silva, B. C. O.
1
; Avelino, C. U. R
1
; Pereira, P. R. S.
1
; Schimieguel, D. M.
1

1
Clinical Laboratory Hematology, Department of Pharmacy of the Federal
University of Rio Grande do Norte (UFRN).
Corresponding author: barbaracoeli@live.com

Introduction: Protein-calorie malnutrition (PEM) is a direct result of
inadequate dietary protein together with a deficient intake of the
carbohydrates and lipids necessary to provide an adequate energy source.
Among the most well documented functional consequences of PEM are
growth impairment, a reduced immune response, and a disruption in
cognitive ability. Moreover, PEM also induces leucopenia, bone marrow
hypoplasia and commitment components of the immune response in
animal model studies. Objectives: Standardization of anesthetic dosage;
standardization of blood sample and bone marrow collection methods,
standardization of hematological techniques in peripheral blood;
standardization of leukocyte count in samples of bone marrow and
induction of malnutrition. Material and Methods: The study employed 14
BALB/c for standardization of anesthesia, peripheral blood (PB) and bone
marrow (BM), hematologic determinations, and induction of malnutrition.
The animals were submitted to protein malnutrition separated by the
control group (CG) and malnourished group (MG). After the initial weight
loss, the animals were anesthetized with sodium thiopental with dose of
150 mg/kg for collecting PB and BM. Results and Discussions: The
results showed no significant difference between the two groups. Average
leukocyte in control group PB:1,45 x10
3
/mm
3
and BM: 1,12 x10
7
/mm
3
; and
malnourished group PB: 1,83 x10
3
/mm
3
and BM: 9,7 x10
6
/mm
3
.
Parameters of red series (average) in control group: hemoglobin: 12,7g/dL;
hematocrit: 45%; erythrocytes: 5,94 x10
6
/mm
3
. Malnourished
group:14,0g/dL hemoglobin, hematocrit 47%, erythrocytes: 4,59 x10
6
/mm
3
.
Conclusion: These preliminary results shows that the test pilots and the
standard technique used create ideal experimental conditions to
performance and continue the project.

Financial support from UFRN/Brazil.


14

CODE 16
REFLECTIONS OF A CUSTODY CHAIN IN CENTER OF
PHARMACEUTICAL EQUIVALENCE

Oliveira, Y. S.; Assis, C. N.
,*
; Lopes, C. C. G. O.

Department of Pharmacy, Federal University of Cear (UFC).
Corresponding author: clarissa.nunes.cna@gmail.com

Introduction: The Custody Chain is used to maintain and document the
movement of the sample in the laboratory from reception to retain custody
in the documentation and archiving. Objective: Evaluate the results
obtained with the implementation of Custody Chain in order to check if
there is an improvement in the quality system of a Center of
Pharmaceutical Equivalence/CPE and if it is in accordance with the
NBR/ISO/IEC 17025/05. Methods: We adopted tools, including:
preparation of protocols, adoption of master log, logbooks for equipment,
documents and forms. It was also provided training for technical staff to
better use the tools mentioned. The measurement of non-compliance was
accomplished through registration on a form specified and validated.
Results and Discussion: Implementation of Custody Chain benefited the
CPE enabling a more efficient tracking of drugs and providing better
maintenance and calibration of equipments and quality of reagents used.
Using these documents, the CPE can justify the amount of samples
requested for quality control analysis. If any error occurred in the analysis,
it was easier to detect the source. It were observed a considerable
decrease of non-compliances(93,7%) and a faster achievement of analysis
without lost in quality of results, allowing an expansion of the number of
tests and customers. There was an increase of credibility with ANVISA and
customers; resulting from the work according to the standards.
Conclusion: The custody chain is a valuable resource for the organization
of samples from a CPE and it is in accordance with the recommended by
the NBR/ISO/IEC 17025/05.


15

CODE 17
NANOCOSMETICS IN PHOTOPROTECTION: DEVELOPMENT AND
CHARACTERIZATION OF NANOPARTICLES WITH SUNSCREENS

Nascimento, D. F.
1
; Silva, A. C.; Freitas, Z. M. F.
1
; Ricci-Junior, E.
1
;
Santos, E. P.
1

1
Departamento de Medicamentos, Faculdade de Farmcia, Universidade
Federal do Rio de Janeiro.
Corresponding author: nuna@ufrj.br

Introduction: As cancer has increased in the last decades,
photoprotection has been largely recommended as a preventive measure
against the harmful results of the ultraviolet radiation on skin. However,
some studies show toxicity associated with sunscreen actives substance,
and the most serious effect is the formation of free radicals. The
sunscreens combined with nanotechnology are interesting because they
decrease the systemic absorption, by improving retention on the most
superficial skin layers. Objective: The purpose of this work was to develop
and characterize poly--caprolactone nanoparticles (Np) with
octilmethoxycinnamate, octocrylene and benzophenone-3. Materials and
Methods: Np were prepared by emulsion/evaporation method. Available
characterization techniques were used for the evaluation of morphology
(TEM), size (DLS), Zeta potencial (ZP) and encapsulation efficiency
(EE%). Results and Discussion: All Np stood at the nanometric scale
changing from 214.5 to 265.6 nm, with Polidispersity Index (PI) from 0.058
to 0.161, with predominantly elliptical and spherical morphologies. The ZP
was slightly negative and changed from -6.05 to -11.01. The values of
EE% changed from 84 to 90%. The content of the actives substances
changed from 44 to 65g/mg of Np. The process yield changed from 60 to
76%. Conclusion: The ZP values obtained suggest good stability of the
system, because it hinders the flocculation. The low PI
valuesdemonstrated the quality of distribution size with a good correlation
between the two techniques (TEM and DLS). The values obtained for yield,
EE% and content were satisfactory, suggesting the applicability of the
method for developing nanocosmetics.


16

CODE 20
ANTIMYCOBACTERIAL ACTIVITY OF THE FRACTIONS AND
COMPOUNDS FROM Scutia buxifolia REISSEK.

Athayde, M. L.
1
; Boligon, A. A.
1
; Cruz, R. C.
1
; Janovik, V.
1
; Agertt, V.
2
;
Campos, M. M. A.
2
; Guillaume, D.
3

1
Phytochemical Research Laboratory at Department of Industrial
Pharmacy, Federal University of Santa Maria (UFSM).
2
Mycobacterial Laboratory Research at Department of Clinical and
Toxicological Analysis, Federal University of Santa Maria (UFSM).
3
Department of Medicinal Chemistry, Universit Reims-Champagne
Ardenne, 51 Rue Cognacq Jay, Reims, France.
Corresponding author: margareth@smail.ufsm.br

Introduction: The use of medicinal plants for the treatment of diseases,
including fungal and bacterial infections is typical in Brazil and worldwide
research groups screen plant extracts to detect secondary metabolites with
antimicrobial properties in an attempt to find new promising compounds.
Material and Methods: The antimycobacterial activity of Scutia buxifolia
(Rhamnaceae) leaves extracts and fractions were evaluated for the first
time against Mycobacterium smegmatis, M. tuberculosis, and M. avium
using the broth microdilution method. The flavonols quercetin and
quercitrin and the phenolics acids gallic and caffeic, were isolated and
further quantified by HPLC-DAD. Results and Discussion: Promising
anti-Mycobacterium smegmatis activity was observed with ethyl acetate
extract (MIC = 312.50 g/mL), whch ws fconed no sc ge
column chromatography and their fractions presented MIC values ranging
fom 78.12 o bove 312.50 g/mL. The fcons III nd VI of S. buxifolia
leaves showed a high level of activity against M. smegmatis (MIC= 78.12
nd 156.25 g/mL, especvey), M. tuberculosis (MIC = 156.25 g/mL)
and M. avium (MIC = 312.50 g/mL), whees o he ohe fcons he
vues ved fom 312.50 o 1250.00 g/mL gns hese sns.
Quercetin and quercitrin were predominantly present in fractions III and VI.
Flavonoids are well known for their antimicrobial potencies, as they can
inhibit enzymes involved in the fatty acid and mycolic acid biosynthesis in
vivo. Mycolic acids are one of the most distinctive features of the
mycobacteria cell wall, essential for its survival. Conclusion: The results
provided evidence that the studied plants fractions might be potential
sources of new antimicrobial drug.

Financial support from CNPq and CAPES, Brazil


17

CODE 21.1
DIRECT INHIBITORY ACTION OF SULFATED POLYSACCHARIDES
FROM RED SEAWEED Hypnea musciformis ON THROMBIN
ENZYMATIC ACTIVITY EVALUATED BY CHROMOGENIC SUBSTRATE
ASSAY

Alves, M. G. C. F
1
.; Dore, C. M. P. G
1
.; Pinheiro,T. S
1
.; Monteiro, N. K. V
1
.;
Costa, T. G
1
.; Leite, E. L
1
.

1
Laboratrio de Glicobiologia, Departamento de Bioqumica,

Universidade
Federal do Rio Grande do Norte (UFRN).

Introduction: Thrombosis is related with cardiovascular diseases one of
the most common in the world and for its treatment is used heparin, a
sulfated polysaccharide used as anticoagulant agent but was observed
some side effects and possible contamination by prions and viruses.
Materials and Methods: In this study, we extracted a sulfated
polysaccharides, fraction 1.0 (F1.0) from red seaweed Hypnea musciformis
and assessment anticoagulant effect by activated parcial thromboplastin
time (aPTT) and prothrombin time (PT) by commercial kits. We also studied
the effect of this polysaccharydes on chromogenic substrate assay of
thrombin and FXa enzymatic activity. Results and Discussion: The
results demonstrated that this fraction was mainly composed of
carbohydrates (49.70 0.10%), with high sulfate content (44.590.015%)
and low protein contamination (0.920.001%) and low phenolic compounds
(4.790.016%). Results obtained by aPTT displays an anticoagulant action
at different concentrations of F1.0 (p<0.001) showing an increasing in the
clot formation time at 25, 50, 100, 150, 200, 250, 350 g. F1.0 showed in all
tested concentrations small clot formation time by PT when compared with
negative control (p<0.05). Using the chromogenic substrate assay of
thrombin and FXa were observed that F1.0 in tested concentrations (150,
250, 350 g) displayed moderated inhibitory action on enzymatic activity of
thrombin (40.00 2.26%, 50.67 2.64% and 45.73 6.60%) and
stimulatory action on FXa (8.51%, 4.93% and 9.70%), respectively.
Conclusion: We conclude that this sulfated polysaccharide (F1.0) showed
low anticoagulant activity determined by aPTT with direct inhibitory action of
thrombin.



18

CODE 21.2
INHIBITORY ACTION ON LIPID PEROXIDATION AND PROTECTIVE
ACTIVITY ON H
2
O
2
-INDUCED HEMOLYSIS OF SULFATED
POLYSACCHARIDES F1.0 FROM RED SEAWEED Hypnea
musciformis

Alves, M. G. C. F
1
.; Dore, C. M. P. G
1
.; Medeiros, H. N
1
.; Paiva A. A. O
1
.,
Filgueira, L. G. A
1
., Rocha, H. A. O
1
., Benevides, N. M. B
2
. Leite, E. L
1
.

1
Laboratrio de Glicobiologia, Departamento de Bioqumica,

Universidade
2
Universidade Federal do Cear
(UFC).

Introduction: The normal metabolic process promotes reactive oxygen
species (ROS) generation, which can induce several pathologic effects.
Antioxidant agents slows or prevents oxidizable cellular substrate oxidation
leading to ROS generation inhibition or scavenging. In their natural habitat,
marine seaweed are exposed to high oxygen concentrations and light,
promoting free radical generation, but they do not suffer any photodynamic
damage, which suggests the presence of antioxidant components.
Objective: In this study was evaluated in vitro antioxidant activity of
sulfated polysaccharides F1.0 from red seaweed Hypnea musciformis.
Results and Discussion: F1.0 was mainly composed of carbohydrates
(49.70 0.10%), with high sulfate content (44.590.015%) and low protein
contamination (0.920.001%) and low phenolic compounds
(4.790.016%). In the lipid peroxidation system induced in egg yolk
sulfated polysaccharides F1.0 promotes the inhibition around 57.92% at
0.08 mg/mL, showing the high inhibition power of F1.0 with IC50 of 0.0030
mg/mL. Sulfated polysaccharides F1.0 at 5 mg/mL promotes 67.89%
hemolysis inhibition with an IC50 of 0.3750 mg/mL in H
2
O
2-
induced
hemolysis model. These polysaccharides F1.0 showed no cytotoxic activity
on erythrocytes in direct hemolysis model, demonstrated by the non-
occurence of significative hemolysis in all tested concentrations. No
statistically significant difference was seen between erythrocytes of the Rh
positive and negative groups or among the erythrocytes of the B, AB, and
O groups. Conclusion: Besides the protective action on H
2
O
2
-induced
hemolysis, sulfated polysaccharides F1.0 does not cause significant
damage action on erythrocyte membranes of different ABO and Rh blood
groups and showed strong inhibitory action on lipid peroxidation assay.



19

CODE 23
DETERMINATION OF ACIDITY IN CURD CHEESE OF CAIC AND
JUCURUTU

Amaral, N. K. M.
1
; Teixeira, L. L.
1
; Silva, A. P. O
1
.; Sousa, P. R. F.
1
.;
Carvalho, B. G.
1
; Moura, M. F. V.
1

1
Programa de Ps graduao em Qumica PPGQ

Introduction: Cheese is a concentrated milk consists of proteins, lipids,
carbohydrates, minerals, calcium, phosphorus and vitamins. The
classification of cheese based on characteristics due to the type of milk
used, the type of coagulation, the consistency of pulp, fat, the type of shell,
healing time, etc. Curd cheese is a dairy product widely produced in the
Northeast region of Brazil. Objective: This work stood the determination of
acidity in the cheese sample of cities Caico and Jucurutu in Rio Grande do
Norte. Material and Methods: We followed the methodology
recommended by the Institute Adolfo Lutz (2008) for determination of
acidity in lactic acid. Results and Discussion: We observed a lactate
curve with four points for each sample, which initially Jucurutu cheese had
the lowest levels, but during the 60 days the positions were reversed.
Comparing the results obtained by Leopoldina (2008) who analyzed
cheese curd Currais Novos presenting results of 0.27 g lactic acid per 100g
of cheese, one can see that the samples analyzed in this study showed
acidity of lactic acid lower that Leopoldina (2008).


20

CODE 24
Toxicologic assay of ethanolic extract from Xylopia cayennensis

Silva, A. D. V.; Pontes, A. C. A. de A.; Campos, D. M.; Da Silva, M. L.;
Nascimento, D.; Martins, M.; Dolabela, M. F.

1
Discente do Curso de Graduao em Farmcia da Faculdade de
Farmcia, Instituto de Cincias da Sade, Universidade Federal do Par
(UFPA).
2
Docente da Faculdade de Farmcia, Instituto de Cincias da Sade,
Universidade Federal do Par (UFPA).
Corresponding author: giza_deni15@hotmail.com

Introduction: Xylopia cayannensis is popularly known as "embira." This
plant is rich in reducing sugars, tannins, catechins, alkaloids, steroids and
triterpenoids, depsides and depsidones. Objective: This study aimed to
evaluate the toxicity from the ethanolic extract of branches and leaves of
Xylopia cayannensis (EEX). Materials and Methods: The lethality test of
EEX on Artemia salina Lech. to obtain the median lethal concentrations
(LC
50
) was performed according to Meyer et al (1982). Twenty Artemia
salina larvae were transferred to test tubes containing artificial sea water
and EEX in six different concentrations. The tests were done in triplicate.
The counting of live and dead larvae was performed after 24 hours.
Results and Discussion: The lowest concentration (0, 46875 mg/mL) had
confirmed mortality larvae between 35% (7) and 40% (8). The intermediate
concentrations (1.875 mg/mL and 3.75 mg/mL) caused mortality of 75%
(15) and 90% (18) respectively. The highest concentrations (7.5 mg/mL
and 15 mg/mL) had of lethality 100% (20). The LC
50
was 0, 9375 mg/mL,
less than 1mg/mL, indicating that EEX presents a high level of toxicity
when compared to the study of the species Xylopia emarginata, in which
was verified LC
50
of 0.127 mg/mL. Conclusions: The methodology with
Artemia salina Lech. was a simple and inexpensive test that provides
results in a short time. However, for a more specific evaluation from the
toxicological activities of EEX, cell-culture toxicological bioassays must be
done.

Financial support from CNPq/Brazil and SESu/MEC/Brazil.


21

CODE 25
ANTIMICROBIAL ACTIVITY EVALUATION OF COPAIBA OIL
MICROEMULSION SYSTEM: A PRELIMINARY STUDY

Alencar, E. N.
1,4
; Xavier - Jnior, F. H.
2,4
; Morais, A. R. V.
3,4
; Silva, K. G. H.
3,4
; Paula, P. R.
4
; Egito, E. S. T.
1,2,3,4

1
Programa de Ps-graduao em Cincias Farmacuticas, Centro de
Cincias da Sade (CCS), Universidade Federal do Rio Grande do Norte
(UFRN), Natal RN.
2
Programa de Ps-graduao em Biotecnologia (Renorbio), UFRN.
3
Programa de Ps-graduao em Cincias da Sade, CCS, UFRN.
4
Laboratrio de Sistemas Dispersos (LaSiD), Departamento de Farmcia,
CCS, UFRN.
Corresponding author: socrates@ufrn.br

Introduction: Copaiba oil is used as a popular anti-inamatory and anti-
infective agent to treat several diseases. However, it is necessary to use a
system that makes its use more pleasant and efficient, such as the
microemulsions. These systems are colloidal dispersions
thermodynamically stable of water and oil stabilized by a surfactant.
Objective: The aim of this work was to perform a preliminary antimicrobial
activity study of Copaiba oil free and loaded into microemulsions against
bacterial and yeasts strains. Material and Methods: The screening test by
modified agar diffusion was performed for all strains and the minimum
inhibitory concentration (MIC) was evaluated by agar dilution, for bacteria
(Staphylococcus aureus, Enterococcus faecalis, Salmonella Typhimurium,
Pseudomonas aeruginosa), and broth microdilution, for yeasts (Candida
albicans, Candida krusei and Cryptococcuss neoformans). Results and
Discussion: The copaiba oil had activity against the yeasts, however the
microemulsions were unable to inhibit the fungal growth. These systems
had a lower MIC value to inhibit Staphylococcus growth lower than the oil.
For example, the MIC of copaiba oil was 1mg/mL and the microemulsion
value was lower than 0.25mg/mL for the same clinical strain of S. aureus.
In short, it can be suggested that microemulsions were able to increase the
Copaiba oil activity against bacterial strains. Also it can be speculated that
these drug delivery system can modify the release and delivery of the oil
compounds for specifics targets. Thus, further study is important in order to
confirm the results on the antimicrobial activity of copaiba oil
microemulsions, which can became a therapeutic choice to treat several
diseases.

Financial support from CNPq and CAPES/Brazil.


22

CODE 26
PROFILE AND QUALITY OF MEDICATIONS PRESCRIPTION IN THE
CITY OF CUIT PB, BRAZIL.

Oliveira, J. R. M. de
1
; Souza, F. B. de; A. D.
1
; Martins, R. R.
1

1
Federal University of Campina Grande - UFCG, Health and Education
Center CES, Cuit-PB, Brazil.
Corresponding author: randmartins@yahoo.com.br.

Introduction: The prescription has a direct action on qualitative and
quantitative aspects of drug use. It is essential that accurate and legible to
avoid errors during interpretation by the patient or health professionals.
Prescription medications must contain the appropriate, correct amount and
proper dosage. Objective: to assess the quality and profile of drug
prescription in Cuit PB, Brazil. Material and methods: the random
sample was made up from 10% of prescriptions issued in the first half of
2010 (1772 prescriptions). The prescribed drugs were categorized
according to the ATC. Results and Discussion: drugs used to treat
hypertension, analgesics and antipyretics, antidiabetics, antibiotics and
anti-inflammatory drug classes correspond frequently. The average
number of drugs per prescription was 1.7. The parameters were: 44.8% of
prescriptions had no concentration, 21.5% lack of pharmaceutical form,
without the amount 8.8%, 14.8% non-dosage, 2.5% without the duration of
treatment and 2 5% without the prescriber's signature. Conclusion:
Overall, the data show serious deficiencies in the writing of prescriptions. It
might be noted that the most representative classes of medications are
drugs for treatment of chronic diseases, antibiotics, analgesics and anti-
inflammatory.


23

CODE 27.1
INVESTIGAO DAS CLASSES METABLICAS DA ESPCIE
BOTNICA Xylopia cayennensis

Da Silva, M. L.; Silva, A. D. V.; Pontes, A. C. A. A.; Campos D. M.;
Nascimento, D.; Dolabela, M. F.

1
Farmcia, Instituto de Cincias da Sade, Universidade Federal do Par.
2
Universidade Federal do Par.
Corresponding author: mallone_rockforever@hotmail.com

Introduction: The botanic species Xylopia cayennensis (XC) is a member
from the family Annonaceae, popularly known as "embira" and is used by
traditional populations for its cardiovascular effects. Objective: This work
aimed to carry out phytochemical approach to the identification of XC
metabolic classes. Materials and Methods: The XC aerial parts (stems
and leaves) were subjected to grinding process to powder obtaining. To
obtain the ethanolic extract (EE), 200g of the powder were measured with
a precision scale and taken to the percolator, which were added 2L of
ethylic alcohol (ratio 1/10). After seven days the EE was removed from the
percolator and filtered. The filtrate was taken to the rotary evaporator to be
concentrated. Then, the EE was placed in a becker, weighed and left in an
heater at 40 C during one week to complete solvent drying and obtaining
of the dry extract (DE). The phytochemical tests were carried out to identify
metabolic classes using the methodology previously described by Barbosa
et al. (2004). Results and Discussion: From all the tests performed during
phytochemical approach, six showed positive results, indicating the
presence of the following metabolic classes: reducing sugars, tannins,
catechins, alkaloids, steroids and triterpenoids, depsides and depsidones.
Some of these classes were previously described in other studies.
Conclusions: There are few studies of the pharmacological activities with
XC, so this first approach was important because it will serve as guidance
for identification tests of XC pharmacological activities.



24

CODE 27.2
Phytochemical and chromatographic analysis of Mikania glomerata

Da Silva, M. L.; Malcher, N. S.; Nascimento, M. S.; Nascimento, J.;
Barbosa, W. L. R.

1
Farmcia, Instituto de Cincias da Sade, Universidade Federal do Par
(UFPA).
2
Discente do Programa de Ps-Graduao em Cincias Farmacuticas,
Instituto de Cincias da Sade, Universidade Federal do Par (UFPA).
3
Corresponding author: mallone_rockforfever@hotmail.com

Introduction: The species Mikania glomerata Sprengel and Mikania
laevigata Schultz Bip are popularly known as "Guaco". However, Mikania
glomerata has been more used as a medicinal plant. These species are
primarily intended for the respiratory diseases treatment. Although, other
pharmacological properties of Mikania glomerata were already reported, as
anti-inflammatory, analgesic, antioxidant, which are associated with the
presence of flavonoids, tannins, saponinas and coumarins (ROCHA et al.,
2008). Objective: Expand the scientific knowledge about Mikania
glomerata, showing the phytochemical approach and chromatographic
character of the extract. Materials and Methods: From the botanical
material tincture were performed phytochemical tests, fractionation of solid-
liquid partition, spectrophotometric analysis and thin layer chromatography
(TLC). Results and Discussion: The phytochemical approach had
positive results for: reducing sugars, phenols and tannins, alkaloids, and
depsides depsidones, and coumarin derivatives. In the analysis by TLC,
the bands formation was observed with the use of the eluents
hexane/acetone and toluene/ dichloromethane/acetone, with the aid of
ultraviolet light at 365 nm and 254 nm; and after revelation through specific
reagents as vanillin and potassium hydroxide (5%). On the
spectrophotometric analysis it was observed that there is a greater light
absorbance when the wavelength is between 300 to 400 nm. Similar
findings were previously observed (BOLINA et al., 2009). Conclusions: It
is possible to conclude that the results corroborate other studies previously
reported in the literature and that the species M. glomerata has been a
great research target due to their pharmacological potential.



25

CODE 28
ZINC OXIDE EMULSION: A NEW ALTERNATIVE TO DIAPER
DERMATITIS TREATMENT

Santiago, R. R.
1,2
; Ferreira, M. R. A.
1
; Silva, K. G. H.
2
; Silva, K. C. H.
2
,
Svidizinsk, T. I. E.
3
; Egito, E. S. T.
1,2

1
2
Laboratrio de Sistemas Dispersos, Departamento de Farmcia,
Universidade Federal do Rio Grande do Norte (UFRN).
3
Laboratrio de Micologia Clnica, Departamento de Anlises Clnicas,
Universidade Estadual de Maring (UEM).
Corresponding author: leninhha@hotmail.com

Introduction: Diaper dermatitis, popularly known as diaper rash, is an
inflammation of the skin covered by the diaper, affecting 50% of
children. Treatment usually involves increasing the frequency at diaper
changing, use of super-absorbent disposable diapers and topical agents
such as creams or barrier creams such as those using zinc oxide (ZnO).
Thus, appears as a new perspective for treatment of diaper dermatitis the
incorporation of zinc oxide in emulsified vehicles where the component of
lipophilic phase had a synergistic effect with the active drug. Objective:
The aim of this work was to develop and to characterize emulsion systems
with sesame oil as lipophilic phase added of zinc oxide. Materials and
Methods: Therefore, pseudo-phase ternary diagrams were used as tool for
identification of viscous emulsion region. Results and Discussion: The
dispersed systems thus obtained were macroscopically classified, and only
seven viscous emulsion formulations were selected for further studies. The
stability of all formulations was analyzed by macroscopic analysis, stability
under storage, stability under centrifugation, freeze/thaw cycles, pH
evaluation, conductivity measurements, particle size analysis and
rheological behavior. After performing the stability studies, the formulation
which was more stable was selected for the addition of ZnO. Conclusions:
Moreover, this formulation showed good results when tested against
reference bacteria and yeasts, demonstrating that it has considerable
antimicrobial activity, particularly against Candida albicans, the
microorganism commonly associated with diaper dermatitis.

Financial support from master scholarship from CAPES/Brazil


26

CODE 30
LEGAL ANALYSIS OF MEDICINES ADVERTISING TRANSMITTED IN
RADIO STATION FROM NATAL/RN ACCORDING TO RDC N 96/08

Batista, A. M.
1
; Carvalho, M. C. R. D.
2

1
Professora Assistente I do Departamento de Farmcia da Universidade
Federal do Rio Grande do Norte (UFRN). Departamento de Farmcia da
Universidade Federal do Rio Grande do Norte (UFRN). Laboratrio de
Monitorao de Propaganda de Produtos Sujeitos Vigilncia Sanitria,
Natal/RN.
2
Professora Associada I do Departamento de Farmcia da Universidade
Federal do Rio Grande do Norte (UFRN). Laboratrio de Monitorao de
Propaganda de Produtos Sujeitos Vigilncia Sanitria. Natal/RN.
Corresponding author: almariamariz@yahoo.com.br

Introduction: Medicines advertising is one of the most important
marketing resources used by the pharmaceutical industry to induce people
to buy these products although they do not have the real necessity to use
them. Objective: To evaluate drugs advertisings transmitted on radio
stations in Natal/RN, from April 2010 to September 2010. Materials and
methods: The capitation of advertising pieces was accomplished through
recording the transmitted programs in 9 radio stations, during 2 hours
transcribed integrally. For that, was elaborated a schedule of transmitted
programs in each station with their respective timetable, in way to include
the 6:00h to 18:00h period. Legal analysis was based on RDC n 96/08
from ANVISA, specific for propaganda control of medicines. Results: The
analysis of 06 captured advertising pieces demonstrates that none of them
accomplishes in full legislation demands; 100% omit active principle;
83,3% omit contraindications; 16,7% announce no registrated medicines in
ANVISA; 16,7% omit indications; 16,7% suggest or stimulate diagnosis.
Conclusions: The study indicates the necessity of the topic drugs
advertisements to be treated in a wide context, that is, to be discussed as a
public health concern. Although the advertising regulation and inspection is
the State responsibility, this should be shared with the advertising agency,
pharmaceutical industry and media. Furthermore, it is indispensable to
inform and to conscience the population of their rights in such mistaken
situation.


27

CODE 31.1
STUDY OF ISONIAZID ADSORPTION ONTO CHITOSAN: ASSAYS OF
EQUILIBRIUM AND KINETICS USING STIRRED TANK

Jcome, R. C.
1
; Gonalves, L.R.B
2
; Adriano, W. S.
1*

1
Laboratrio de Cincias Farmacuticas, Centro de Educao e Sade,
Universidade Federal de Campina Grande (UFCG).
2
Laboratrio de Processos Biotecnolgicos, Departamento de Engenharia
Qumica, Universidade Federal do Cear (UFC).
Corresponding author: wellington.adriano@ufcg.edu.br

Introduction. Tuberculosis is a health problem in Brazil and other twenty
one countries, which, together sum 80% of all cases in the world. For the
treatment of this disease, isoniazid remains the drug of choice. Adsorption
has been used as one of the promising techniques seeking low-cost
adsorbents with high adsorption capacity. Objective. Thus, objective was
to investigate isoniazid adsorption on chitosan promoting an alternative
using of regional products as adsorbents and bringing potential social-
economic benefits by developing an innovative technology. Material and
Methods. Maximum adsorption capacity isotherm was determined from
tests in stirred tank trough Langmuir`s isotherm, using isoniazid prepared
in 20 mM sodium acetate buffer, pH 4.0 at different concentrations, with
constant mass of adsorbent during one hour, being carried out kinectics
assays and desorption tests. Results and Discussion. Results showed
that Langmuir isotherm model fitted to experimental datum with maximum
adsorption capacity of 55.2mg of isoniazid per gram of adsorbent and
dissociation constant of 0.20mg.mL
-1
being kinetic process about 10 min
which facilitates process of drug separation. Previous desorption assays
showed isoniazid releasing under high ionic strength condition (NaCl 2M).
Conclusions. Use of chitosan is feasible for the isoniazid separation
process by adsorption, as their preparations are simple, presenting low
cost combined with high adsorption capacity.

Financial support from CNPq/Brazil.


28

CODE 31.2
HOUSEHOLD SURVEY ABOUT THE PROFILE OF DRUG USE BY
ELDERLY IN THE CITY OF CUIT-PB.

Jcome, R. C.
1
; Almeida, M. A. A. L. S.
1
; Batista, M. T.
1
; Martins, R. R.
2
;
Nagashima Jr, T.
2

1
Discentes do Curso de Bacharelado em Farmcia, Centro de Educao e
Sade, Universidade Federal de Campina Grande (UFCG), Cuit-PB.
2
Docentes do Curso de Bacharelado em Farmcia, Centro de Educao e
Sade, Universidade Federal de Campina Grande (UFCG), Cuit-PB.
Corresponding author: rosalina_coelho@hotmail.com

Introduction. The population aging is a global phenomenon that has
repercussions on changing habits and behaviors. In this context the elderly
need new assistive technology for the maintenance of health care and for
these cases, drugs are being widely used Objective. The aim of this work
was to evaluate the profile of drug use by elderly people residing in Cuit-
PB. Material and Methods. An observational and descriptive study carried
out in 60 households randomly selected. Data collection took place through
individual structured interviews realized in april/2010 and data analysis
used the software excel 2007. Results and Discussion. The sample was
composed of 54 interviewed aged between 60 - 95 years. Results shows
that there was an average of 3.7 person by house living of which 27.5% of
them were elderly. Was observed among the elderly, low income and low
educational level, these factors may be related to inappropriate use of
medicines. The classes of medications most prevalent by elderly people
were the anti-hypertensive (34.8%), analgesics and anti-inflammatory
drugs (23.2%) and hypoglycemic (12.5%). Conclusions. This work
demonstrates that the chronic use of drugs is associated with a low income
and low educational levels. This information is essential for adequate
planning of health care, contributing to the rational use of medicines.


29

CODE 32
ORAL ADMINISTRATION OF EXTRACT CYANOBACTERIAL BLOOM
IN PREGNANT RATS: A REPRODUCTION ASSESSMENT

Almeida, C. S.
1
; Arruda, A. C. C.
1
; Barbosa, P. F.
1
; Dantas, D. H. M.
1
;
Costa, H. T. M. L.
1
; Costa, I. A. S. O.
2
; Lemos, T. M. A. M.
1
; Schwarz, A.
1
;
Pinto, E.
3
; Kujbida, P.
1

1
Laboratrio de Toxicologia, Departamento de Anlises Clnicas e
Toxicolgicas, Universidade Federal do Rio Grande do Norte (UFRN).
2
Laboratrio de Ecologia e Toxicologia de Microrganismos Aquticos,
Departamento de Microbiologia e Parasitologia, Universidade Federal do
Rio Grande do Norte UFRN).
3
Laboratrio de Toxinas e Produtos Naturais
de Algas, Departamento de Anlises Clnicas e Toxicolgicas,
Universidade de So Paulo (USP).
Corresponding author: cristhianosibaldo@hotmail.com

Introduction: Lots of researches have been focused on the pure
cyanobacterial toxins, but little on the natural cyanobacterial bloom.
Objective: This study was undertaken to investigate the effect of the
natural cyanobacterial bloom extract by oral via (gavage) on the female
reproductive performance. Materials and Methods: Hydrophilic Interaction
Chromatography/ fluorescence Jetection and Liquid
Chromatography/tandem mass spectrometry toxin analyses were
performed on surface water samples from 37 reservoirs in the Rio Grande
do Norte and some microcystins (MCs) congeners were detectable in 14
samples. Sum of all variants the values of MCs varied from 0,22 to 51,86
ng/mL. It was used the most concentrated sample to exposure adults
females Wistar rats. The extract of cyanobacteria bloom was administered
at first gestational day (GD) to 4 different groups (10/group) in different
doses (0, 250, 100 and 40 g MCs/ Kg body weight/day. At GD 20 the rats
were euthanized for biochemical and reproductive studies. Results and
Discussion: The statistical analysis showed absence of alterations at body
weight, body weight gain, food and water intakes, organ weight/body
weight ratios and at AST, ALT, creatinine and urea serum levels in both
studies. The reproductive performance (the number of implanted embryos,
pre-implantation loss, resorptions, corpora luteo, implantation, live and
dead offspring) was similar between control and expepemental dams.
Conclusions: These findings suggest that the toxic cyanobacterial bloom
administered at this concentration of MCs during gestation is not able to
promote maternal toxicity and to impair gestation or reproductive
performance of rats.



30

CODE 33
COMPARATIVE ANLYSIS OF PHYSICAL AND CHEMICAL
PROPERTIES OF TWO SOURCES OF WATER FOR USE ON SCHOLL
PHARMACY MANUEL CASADO ALMEIDA, CUIT-PB

Lima, D. D. F. S
1
; Lima, W. S.
1
; Alencar, J. S.
1
; Silva, P. S. G.
1
; Almeida,
M. A. A. L. S.
1

1
Universidade Federal de Campina Grande / Centro de Educao e
Sade, Unidade Acadmica de Sade, Oho Dgu d Bc, s/ n, Cuit,
PB, Brasil 58175-000.
Corresponding author: daisyformiga@gmail.com

Introduction: According to the RDC 67/07, the water used in the
manipulation must be obtained from drinking water, treated in a system
that ensures the attainment of water with pharmacopoeial specifications for
purified water. Objective: The study aimed to the physical and chemical
analysis of two different types of water by comparing them with a
benchmark to determine the font to be used for treatment and use in the
scholl pharmacy Manuel Casado Almeida. Materials and Methods:
Analysis were performed with natural spring water and tap water, and
deionized water as standard. The methodology used was to measure pH,
turbidity, conductivity, content total solids and content chloride. Results
and Discussion: The analysis showed values of pH approximate between
tap water and deionized water, and 6,73 and 6,90, different from the
natural spring that was 3,89. In the determination of conductivity, total
solids and chlorides, the natural source and tap water obtained similar
values, according to the following values: 2.65 and 2.72 mS /cm 0.20g and
0.18 g and 40.64 g/100 ml and 40 , 07 g/100 ml respectively. The turbidity
of the 2.25 NTU natural source was lower than 5.86 NTU tap
water. Conclusions: The results have properties compatible between the
two waters, differing in the aspects of pH and turbidity, confirming the
economic viability of using natural source for the supply. Corrections of the
parameters of chosen water can occur through interventions in the process
of purification that meets the quality specifications required by law


31

CODE 34.1
QUALITATIVE TESTS OF AMPICILLIN SODIUM POWDER FOR
INJECTION

Ttoli, E. G.
1
; Salgado, H. R. N.
1

1
Faculdade de Cincias Farmacuticas, Universidade Estadual Paulista
(UNESP)
Corresponding author: eliane.totoli@gmail.com

Introduction: Ampcn sodum s -lactam antibiotic for parenteral use,
belonging to the penicillin group. Ampicillin was the first semi-synthetic
penicillin that showed activity against Gram-negative bacteria, because of
its greater ability to penetrate the outer membrane of these micro-
organisms, opening the field of broad-spectrum penicillins. Objective: This
study aims to develop qualitative tests for ampicillin sodium injectable.
Materials and Methods: The tests performed with ampicillin sodium
powder for injection were as follows: solubility (in solvents with different
polarities), determination of average weight, moisture content
determination (by an infrared moisture balance), pH determination and
thin-layer chromatography (TLC) (mobile-phase: ethanol : water, 80:20 /
stationary phase: silica). Results and Discussion: It is easily soluble in
water, acetic acid 0.1 M, sodium hydroxide 0.1 M and hydrochloric acid
0.1M. In addition, it is soluble in methanol and slightly soluble in ethanol.
However, it is insoluble in dichloromethane, acetonitrile, ethyl ether,
chloroform, acetone, ethyl acetate and butanol. The average weight of the
vials is in accordance to specifications. It has 2.87% 0.04% of moisture.
The determination of pH showed that ampicillin sodium injectable is basic,
with pH of 8.72. The Rf value for the TLC is 0.84. Conclusion: The tests
performed are excellent to identify ampicillin sodium powder for injection.
Qualitative methods are essential because they allow determining if the
product is in accordance to specifications and they also enable the
realization of predictions for quantitative methods.

Finalcial support: FAPESP, CNPq, PADC-FCF and Unio Qumica


32

CODE 34.2
DEVELOPMENT AND VALIDATION OF TURBIDIMETRIC ASSAY TO
POTENCY EVALUATION OF AMPICILLIN SODIUM FOR INJECTION

Ttoli, E. G.
1
; Salgado, H. R. N.
1

1
Faculdade de Cincias Farmacuticas, Universidade Estadual Paulista
(UNESP)
Corresponding author: eliane.totoli@gmail.com

Introduction: Ampicillin sodium is a semi-synthetic -lactam antibiotic for
parenteral use, belonging to the penicillin group. Microbiological assays are
described for several antimicrobial agents, because they make possible to
check the potency of the product against a microorganism. Objective: The
objective of this study was to develop and validate simple, sensitive, a
precise and accurate microbiological assay by turbidimetry, to quantify
ampicillin sodium injectable. Materials and Methods: In six test tubes
connng 10 mL of TSB boh, ws dded 600 L of a Staphylococcus
aureus 25923 suspension, standardized to 25% transmittance in
specophoomee (580 nm). In hee of hese ubes ws dded 200 L of
sndd souon nd n he ohe hee, 200 L of smpe souon he
same concentrations of standd souons (2.0; 4.0 nd 8.0 g/mL). I ws
performed in triplicate. The test-tubes were incubated in a Shaker, in water
bath, for 4 hours, and then the absorbances were checked in a
spectrophotometer (530 nm). A curve of absorbance by the log of
concentration was constructed. Results and Discussion: The potency
verified was 97.82%. The method showed linearity, with correlation
coefficients of 0.9999 (standard) and 0.9998 (sample); accuracy (mean
recuperation = 100.76%); precision inter-assay (R.S.D. = 0.76%) and
robustness. Conclusion: The potency method was validated and showed
to be adequate for determining the of ampicillin sodium injectable. In
addition, it is fast, easy, economical, and can be used in routine analysis of
Quality Control.

Finalcial support: FAPESP, CNPq, PADC-FCF and Unio Qumica


33

CODE 35
TECHNICAL AND ADMINISTRATIVE MANAGEMENT OF SCHOOL
PHARMACY'S WAREHOUSE MANOEL CASADO DE ALMEIDA
UFCG/CES.

Medeiros J. R. I. J.
1
; Lima, W. S.; Alencar, J. S.

Farmcia Escola Manoel Casado de Almeida, Curso de Bacharelado em
Farmcia, Universidade Federal de Campina Grande/Centro de Educao
e Sde, Oho Dgu d Bc, s/n, Cu, PB, Bs.
Corresponding author:ivson_medeiros@hotmail.com

Introduction: The School Pharmacy, besides acting as an academic
support, should, in fact, have returns for their maintenance and
competitiveness with other establishments present in the town of Cuit
PB. To do so, s necessy o eso o mngemen oos n ode o
reduce production costs and obtain products with good quality. One way to
achieve this is the stock management related to the implementation of the
Good Manufacturing Practice s in Pharmacy (GMPs). Objective: Given
this fact, this study aimed to plan and organize the warehouse of the
School Pharmacy Manoel Casado de Almeida UFCG/CES. Material and
Methods: Following the Resolution No. 67, October 8, 2007 ANVISA
which establishes the GMPs in pharmacies, the layout of the school
pharmacy was designed within the required standards and Standard
Operating Procedures (SOPs) of the cleaning and material inflow and
outflow. Results and Discussion: The layout of the school pharmacy is a
key to the development of GMPs, once that a physical structure facilitates
proper working conditions and avoids storage of pharmaceutical input.
SOPs designed to ensure integrity of the received and stored material,
which along with the stock quantitative management (curve ABC) can
mnmze he o cp nvesed n sock. Is expeced h wh he
implementation of management measures, the costs may be reduced and
that the material received may be in ideal conditions for use, thus providing
more competitiveness in pharmacies compared to other pharmacy outlets.


34

CODE 36
VALIDATION OF A SPECTROPHOTOMETRIC METHOD (UV-VIS) FOR
DETERMINATION OF TOTAL FLAVONOIDS OF THE RED PROPOLIS
FROM ALAGOAS: USE OF CATECHIN AS BIOMARKER

Oliveira, J. M. S.
1
; Silva, L. R.
1
; Neto, J. C
1
.; Arruda, R. E. S.
1
; Nascimento,
T. G.
1
; Junior, I. D. B.
1

1
Laboratrio de Anlises e Controle de qualidade de Frmacos e
Medicamentos, Grupo de Pesquisa em Tecnologia e Controle de
Qualidade de Medicamentos,Curso
de Farmcia, Escola de Enfermagem e Farmcia, Universidade Federal de
Alagoas (ESENFAR/UFAL) Macei, Alagoas.
Corresponding author: jose_marcos_cbjr@hotmail.com

Introduction: The red propolis obtained from mangroves region of Alagoas
presents organoleptic, chemical and biological characteristics, which are
different from other types of propolis from Brazil. Currently, the
phytotherapy industries have necessity to develop low cost methods and
quick for quality control assays of propolis. OBJECTIVE: The aimed of this
work was to develop and validate a spectrophotometric method (UV-vis) for
determination of total flavonoids of red propolis. Material and Methods:
Catechin was purchased from Sigma-Aldrich (98%). Propolis (in nature)
was collected in the region of mangroves in Marechal Deodoro city.
Tincture of propolis (20%) was obtained after extraction by maceration and
concentration in rotaevaporador. Catechin standard solution (2000g/mL)
nd ncue of popos (2000g/mL) wee peped n ehno nd dued
o concenon nge of 8.5 o 85g/mL nd 5.0 o 70 mg / mL,
respectively, for purpose of establish parameters of validation described in
the RDC 899/2003 of ANVISA. Results and Discussion: The
spectrophotometric profile of tincture and sub-fractions of red propolis was
similar to catechin with maximum wavelengths of 212 and 280nm. The
method proved to be easy turn and quick execution, besides to present
excellent selectivity and adequate limit of quantification for phytotherapics
and opotherapics. The method showed linearity and less variation (2.6%
and 6.9%) of intermediate precision and accuracy (3.5% and 7.1%) for
catechin and tincture of propolis, respectively. Conclusion: The UV
method complies with the validation requirements. Catechin may be a
marker for determination of total flavonoids of red propolis.



35

CODE 37.1
CONSTITUENTS STUDY OF FIXED Pike NORTHEAST (Caryocar
coriaceum Wittmer.)

Castro, K. M.
1
; Rodrigues, N. R.
1
; Bandeira, M. A. M.
1

1
Laboratory of Pharmacognosy, Department of Pharmacy, Federal
University of Cear (UFC).
Corresponding author: keinemc@hotmail.com

Introduction: Piquizeiro tree size is a robust Caryocar belonging to the
genus with about 20 different species. In Brazil, the species occurs on
Wittmer Caryocar coriaceum Cariri (CE ). In addition to its importance in
food, is widespread in folk medicine the use of regional peak oil added to
the honey against colds and bronchitis. In our previous work we
determined the major fatty acids that make up this oil with their respective
levels (palmitic, oleic acid and 27.87%, 65.71%) and also the presence of
carotenoids.Objective: To study the constituents of the fruits fixed in order
to determine bioactive chemical constituents. Methodology: The ripe fruits
were collected in the mountains of Araripe, Cariri_CE region. For the work
of isolating the chemical constituents fixed prepared the ethyl acetate
extract through cold maceration epicarp and mesocarp.Evaporated at room
temperature. Purified extract was obtained by chromatographic column of
silica gel using as eluent dichloromethane and acetone in increasing
polarity.The obtained fractions were analyzed by thin layer
chromatography, collected and coded. Results: The fraction F (10-13)
presented in the form of white solid, which was subsequently purified on
Sephadex using methanol as eluente.Houve prominently in fractions that
contained a white crystalline solid, coded as F (7). Conclusion: The
spectra of RMN1H, RMN13C, DEPT, HMQC and HMBC of this substance
show that it was a triterpene.


36

CODE 37.2
GUIDANCE ON PHARMACEUTICAL PREPARATIONS MEDICINAL
PLANTS WITHHOMEMADE: A STRATEGY FOR FAMILY HEALTH

Sousa, L. C.
1
; Pinto F. A.
2
; Castro, K.M.
1
; D. Marino, R. S.
1
; Flag, M. A. M.
1

1
Department of Pharmacy, Dean of Extension ( UFC) Fortaleza, Brazil.
2
Prefeitura Hall of Fortaleza, Fortaleza, CE, Brazil.
Corresponding author: keinemc@hotmail.com

Introduction: Project's CEDEFAM Phytotherapy (Center for Family
Development) came the need for drugs in the community do Planalto do
Pici and was structured to providepharmaceutical care in the field of Herbal
Medicine in Public Health. It consists of a Garden of Medicinal Plants,
which consists of plants that have proven efficacy and safety, based on
the Project of Living Pharmacies UFC (FJA Matos). The present work aims
to develop a strategy for the health of the family through
guidance onpharmaceutical preparations homemade herbal. Methods:
The guidance on pharmaceutical preparations was performed using
homemade leafletindicated for each medicinal plant and type of
preparation. At the time of guidanceusers received free plant material fresh
and joined. Results conclusions: The preparation was the most suitable
home for leaking chamber. Other preparationswere also targeted: basil
infusion, infusion of eucalyptus, lemongrass tea among others. Most
people who received such guidance were mothers (89%). Medicinal plants
for being part of the therapeutic family arouse interest and community
acceptance for this type of guidance pharmaceutical checking for their
scientific importance.


37

CODE 38
STUDY OF THE TOXICITY OF ESSENTIAL OIL OF Cymbopogon
citratus (DC) STAF. FOR A POSSIBLE APPLICATION AS
ANTIFUNGAL DRUG

Vasconcelos, L. B.
1
; Carmo, E. S.
1,2*
, Lima, I. O.
2
; Estevan, E. C.
2
; Gayoso,
C. W.
3
; Diniz, M. F. F. M.
4
; Arajo, M. S. T.
4
; Lima, E. O.
2

1
Academic Unit of Health, Center Education and Health, Federal University
of Campina Grande (UFCG).
2
Program of Pos-Graduation in Natural and Syntetics Bioactive Products,
Pharmaceutical Technology Laboratory, Health Sciences Center, Federal
University of Paraba (UFPB).
3
Department of Dermatology, Center of Health Sciences, Federal
University of Paraba (UFPB).
4
Laboratory of Toxicology, Department of Pharmaceutical Sciences, Center
of Health Sciences, Federal University of Paraba (UFPB).
Corresponding author: laricevasconcelos@hotmail.com

Introduction The number of bacterial and fungal infections has increased
worldwide, thus the antimicrobial available in the market have become
insufficient, especially by the increasing number of resistant
microorganisms. In this context, researches have been conducted in order
to investigate more effective and low toxicity alternative therapies, like the
pre-clinical trials developed with the essential oil of Cymbopogon citratus,
oil with antimicrobial activity in vitro scientifically proven. Objective This
study was conducted to provide data on acute toxicity and skin irritation in
animals exposed to the essential oil of Cymbopogon citratus for a possible
application this as antifungal drug. Materials and Methods The acute
toxicity test was carried in two groups of six mice (Mus musculus) male and
female who received a dose of 2000 mg/kg (oral). To the skin irritation
experiment, we used albino rabbits that received topically 0.5mL dose of
1.25 L/mL of the same oil and were observed for 72 hours, to the
presence of edema, erythema or pressure ulcers. Results and
Discussion No deaths or significant differences in body weight and organs
weight between control and treated animals. Mice showed no changes in
general appearance, behavioral or physiological activities significant. No
sign of skin irritation in rabbits was observed. Conclusions Data presented
contribute to a possible safe use of the essential oil of C. citratus how
antifungal in the future.


38

CODE 39.1
PRELIMINARY STUDY OF THE TOXICITY OF Aspidosperma excelsum
BENTH (APOCYNACEAE).

Gomes, L. F. S.
1
; Dolabela, F.
1
; Moares,.
1
,Barbosa, W. L. R
1
,Vasconcelos,
F.
1

1
Programa de Ps-Graduao em Cincias Farmacuticas, Instituto de
Cincias da Sade, Universidade Federal do Par.
Corresponding author: lfabio@ufpa.br

Introduction: The genus Aspidosperma (Apocynaceae) occurs from
Mexico to Argentina and is distinguished by the frequent occurrence of
indole alkaloids. Amazon is a species used by indigenous and mestizo for
various medicinal purposes (anti-inflammatory, fever and cancer, among
others) and several indications as potent antimalarial agent. It has been
reported the toxicity of A. pyrifolium Mart in goats and has also been
demonstrated in mice the toxicity of A. subincanum. and was also reported
that A. pyrifolium, was able to induce abortion in rats and cause
pathological changes. Objective: Evaluate the cytotoxicity of the
hidroethanolic extract of the A. execelsum Benth (HEEAs) and alkaloidal
fractions.Perform the test for acute oral toxicity of the HEAs. Materials and
Methods: HEEAs preparaition: 500g powder from the bark of the trunk of
A. excelsum Benth, were kept in ethanol 70 v/v for 10 days.After removal
of the ethanol the hydroethanolic extract of the A. excelsum Benth
(HEEAs) was lyophilized and were prepared two fractions, methanol and
alkaloid. Cell culture: The effects citotoxic of the HEEAS was performed in
cultured fibroblast L929 cell line. L929ces wee mnned n Dubeccos
modfed Eges mdum suppemened wh 10% fe cf seum nd
antibiotics (100units/ml of penicillin and 100g/ml of streptomycin)
streptomycin), at 37C in a humidified atmosphere of 5% CO
2
. Citotoxicity
assay: The viability cell was performed by colorimetric assay MTT,
according to the method of Mosmann. L929 cells were incubated (24h,
37C and 5% CO
2
) into 96-well plates. This study used the HHEAs and
alkaloidal fraction at the concentration of 0.4, 0.8, 1.6, 3.12 and
6.25mg/mL. Absovance was measured in an ELISA microtiter plate reader
at 570nm. Viability was defined as ratio (%) of absorvance o treated cells
to untreated cells. Acute Oral Toxicity: Was done according to OECD and
adopted the fixed dose procedure. Results and discussion: Only C2
alkaloidal fraction showed a small but significant cytotoxicity (concentrations
higher than 800 g/mL). The extract not only cytotoxicity but also did not
showed any obvious sign of toxicity in acute oral dose of 5000 mg/mL,
indicatiing that these preliminary results , the extract did not show any
apparent toxicity. Conclusion: The HEEAs was not cytotoxic, and also did
not cause any apparent toxicity.

Financial support: FAPESPA and CAPES.


39

CODE 39.2
ACUTE ORAL TOXICITY EVALUATION OF THE DRY ETHANOLIC
EXTRACT OF Himatanthus articulatus AND Eleutherine plicata

Vilhena, T. C.
1
; Silva, T. L.
1
; Gomes, L. F. S.
2
; Vasconcelos, F.
3
; Percrio,
S
4
; Pereira, W. L. A
5
.

1
Mestrando no Programa de Ps-Graduao em Cincias Farmacuticas,
Instituto de Cincias da Sade, Universidade Federal do Par, Belm-Pa.
2

Mestre em Cincias Farmacuticas pelo Programa de Ps-Graduao em
Cincias Farmacuticas, Instituto de Cincias da Sade, Universidade
Federal do Par, Belm-Pa.
3
Laboratrio de Toxicologia, Faculdade de
Farmcia, Universidade Federal do Par, Belm-Pa.
4
Laboratrio de
Pesquisa do Estresse Oxidativo, Instituto de Cincias Biolgicas,
Universidade Federal do Par, Belm-Pa.
5
Laboratrio de Patologia
Veterinria, Universidade Federal Rural da Amaznia, Belm-Pa.
Corresponding author: lfabiofarmaco@yahoo.com.br

Introduction: In Brazil, the use of natural products for medicinal purposes
dates from before its colonization. The natives used these products for
healing and in different religious rituals. Today, this knowledge have been
adapted to the needs of the urban populations that acquire these products
in markets, and is attracting the attention of researchers who studies the
therapeuct potentials of natural products, as well as the identification and
isolation of bioactive substances. In this context, products obtained from
Sucuuba (Himatanthus articulatus) and Marupazinho (Eleutherine plicata)
are used for the treatment of malaria and amoebiasis, respectively; but
there are only a few studies of the toxic effects of these species, an
important item in the evaluation of the security in the use of medicinal
plants. Objective: Evaluate the acute oral toxicity of the dry ethanolic
extract from the barks of H. articulatus (EEHa) and bulb of E. plicata
(EEEp). Matherial and Methods: The barks of H. articulatus were
collected in Marapanim/PA, in the month of june; the bulbs of E.plicata
were collected in Traquateua/PA in September; both of them were
identified by researcher of the Emlio Goeldi Museum, Dra. Marlia Coelho
Ferreira. After the collection, the materials were washed, dried and
pulverized, and then a aliquot of the drug from both species was used in
the pharmacognostic and physicochemical tests. Next, 1kg of each drug
were used for the extraction of the metabolites. The method of choice was
percolation in 5 L of alchohol 96 GL. The obtained extracts were filtered
and concentreted in rotary evaporator. A part of these was used in
phytochemical analisys, and the other part was lyophilized. With the EEHa
and EEEp, was made the evaluation of the acute toxicity, in Swiss young
mice from both genders, following the OECD 420. Results and
Discussion: The EEH nd EEEp (5000 mg/kg) ddn show ny sgn of
toxicity in mice, except for the diarrhea cases in the animals treated with
EEHa, on the firsts 24 hours of tests, which was corroborated by the
absence of hystopathological modifications. Conclusion: The EEHa and
EEEp showed little toxicity on the major dose used on the test.

FINANCIAL SUPPORT: master scholarship from CAPES/Brazil


40

CODE 40.1
HISTOPATHOLOGICAL STUDIES OF THE EFFECTS OF THE
AQUEOUS EXTRACT OF THE Orbignya phalerata Mart (BABASSU)
MESOCARP POWDER IN ADULT MICE TREATED ORALLY

Silva, A. P. S.
1
; Barros, I. C
1
; Colho, M. L.
1
, Rios, V. M
2
; Policarpo, P. R
2
;
Arajo, S. S.
3
; Borges, E. M. M.
3
; Tom, A. R.
4
; Nunes, L. C. C.
1*
; Freitas,
R. M.
1*

1
Laboratory of Experimental Neurochemistry Research, Pharmaceutical
Technology Center, Federal University of Piau. (NTF/LAPNEX/UFPI).
2
Pharmaceutical Technology Center, Laboratory of Experimental
Neurochemistry Research, Federal University of Piau
(NTF/LAPNEX/UFPI).
3
Laboratory of Experimental Neurochemistry Research, Federal University
of Piau (NTF / LAPNEX / UFPI)
4
State University of Cear (UECE).
Corresponding author: rivmendes@hotmail.com

Introduction: The babassu mesocarp (Orbignya phalerata Mart) produces
a powder that is commonly used among communities in the Northeast of
Brazil as medicine and food supplement, yet without the existence of data
on its efficacy and safety for human consumption. Objective: The effects
of the acute oral administration of the powder aqueous extract (PAE)
obtained from the mesocarp of babassu were investigated through the
histopathological analysis in adult Swiss mice. Materials and methods:
The animals (n=10/group) were divided in four groups and were treated
orally with doses of 1, 2 and 3 g/kg with the PAE, respectively group 1, 2
and 3 and, to the fourth group, the control group, a 0.9% saline solution
was administered. After 30 days of observation, all groups were euthanized
and their brains were removed for histopathological analysis performance.
The severity of the injury was expressed as mean SEM (standard error of
the mean) of the scores of damage. Results and Discussion: The groups
treated with babassu PAE were not able to promote injuries worthy of note,
as the neurons of the hippocampus region presented morphology in the
pattern of nerve cells, with spherical nuclei and central nucleoli, with no
vacuolization or polymorphisms. The areas of the striatum were well
characterized, without areas of necrosis or vacuolization. There were no
significant differences between the test groups and the control group.
Conclusions: Thus, under the conditions of the study, the babassu PAE
did not produce toxic effects in the animals exposed to it.


41

CODE 40.2
THERMAL CHARACTERIZATION OF BABASSU (Orbignya phalerata
Mart) MESOCARP POWDER COMPARED WITH THE RAW MATERIAL
MICROCRYSTALLINE CELLULOSE

Barros, I. C.
1
; Silva, A. P. S
1
; Colho, M. L.
1
, Policarpo, P. R.
2
; Freitas, R.
M.
1*
; Nunes, L. C. C.
1
1
Post-Graduation Program in Pharmaceutical Sciences, Pharmaceutical
Technology Center, Laboratory of Experimental Neurochemistry Research,
Federal University of Piau. (NTF/LAPNEX/UFPI), Teresina - Piau, Brazil.
2
Scientific Initiation Program - Bachelor of Pharmacy, Pharmaceutical
Technology Center, Laboratory of Experimental Neurochemistry Research,
Federal University of Piau (NTF / LAPNEX / UFPI), Teresina Piau,
Brazil.
Corresponding author: liviocesar@hotmail.com

Introduction: The thermal analysis is used in the pharmaceutical industry
as a fast and reliable technique on the development of new pharmaceutical
formulations. Objectives: The thermal characterization of the powder of
babassu mesocarp (BM) (Orbignya phalerata Mart.) and the comparison
with the microcrystalline cellulose. Materials and methods: The curves of
differential thermal analysis (DTA) and the thermogravimetric analysis (TG)
were obtained by a differential thermal analyzer in an atmosphere of N
2
,
flow of 50mL.min
-1
and a heating rate of 10C.min
-1
up to 600C. Results
and discussion: The BM DTA curve showed an endothermic process in
the range of 30C to 80C (humidity loss) and two sequential endothermic
processes in 283.40 to 348.12C and from 424,09 to 456.52C
(decomposition of the material). On the BM TG, three events of mass loss
at temperatures corresponding to the DTA were observed. In the DTA of
cellulose, a loss of surface water in 39.08C to 93.94C (confirmed by TG)
and another endothermic event around 370C (decomposition of the
excipient) were detected. On the TG, a large mass loss (65.57%) of
342.47C up to 388.40C and the decomposition of the rest of the material
in the range of 388.40 to 571.15C were noted. Thus, it was perceived
the superposition of some events. Conclusions: The babassu mesocarp
presented thermal characteristics similar to the microcrystalline cellulose.


42

CODE 41
STUDY AND CHARACTERIZATION SPECTROSCOPIC OF THE
COMPLEX POTASSIUM HEXACYANOFERRATE

OLIVEIRA, S. D. S.; TEXEIRA, L. L.; PONTES, D. L.

Introduction: Coordination or complex compounds are substances that
have a central atom or ion, bound to atoms, ions or groups known as
ligands. His study is necessary due to its diverse applications. A very
important ligand in the coordination chemistry is the cyanide ion (CN-).
Being a strong ligand, together with the carbonyl originates stable
complexes with well-known low-spin configuration, for example,
hexacyanoferrates. Objective: This work aims to study the spectroscopic
characteristics (UV-Vis and IR) of the iron complex: K3[Fe(CN)6] as well as
carry out the study of symmetry. Material and Methods: Applying the
symmetry operations were able to identify the point group to which the
compound belongs. Using this information, it has been obtained the
theoretical number of active bands in the infrared region for the complex
studied. The vibrational spectrum of the complex K3[Fe(CN)6] has shown
only one band n(CN) at 2122 cm-1. The electronic spectrum of the
complex K3[Fe(CN)6] 7 has shown absorption bands: 205, 262, 281, 303,
325, 404 and 429 nm. Conclusion: It has been concluded that the
complex K3[Fe(CN)6] presented the same number of bands in the infrared,
obtained in theoretical study of the symmetry group.


43

CODE 42
MEASUREMENT OF TOTAL FLAVONOIDS IN THE
HYDROALCOHOLIC EXTRACTS OF RED PROPOLIS FROM ALAGOAS

Marta, M. F. S
1
; Firmino, T. T. D.
1
; Vieira, D. A.
1
; Azevedo, E. P.
1
;
Nascimento, T. G.
2
; Diniz Baslio, I. Jr.
1

1
Laboratrio de Tecnologia e Controle de Medicamentos, Escola de
Enfermagem e
Farmcia, Universidade Federal de Alagoas (LabTCon/ESENFAR/UFAL).
2
Laboratrio de Controle de Qualidade de Medicamentos, Escola de
Enfermagem e Farmcia, Universidade Federal de Alagoas.
Corresponding author: tassia_tatianna@hotmail.com

Introduction: Propolis presents a mixture of gum, resins and balms
originated form diferents vegetals sourcers. Propolis stands out for its
antibacterial, antifungal, antiviral, cytostatic and immunostimulant activity
which justify its utilization since remote times.Objective:The work
consisted in the application of the Spectrophotometry of UV-VIS in order to
quantify of total flavonoids in hidroalcoolic extracts of red propolis from
Alagoas using spectrophotometry UV-VIS. Materials and Methods:The
red propolis was obteined from apiaries of mangrove region of Marechal
Deodoro city. The validation of the method of analysis of extracts by
ultraviolet spectrophotometry was made after the determination of the
total flavonoids. To validate the methods were respected parameters of
linearity, interval, accuracy, specificity, accuracy, robustness, limit of
quantification and detection limit. Results and Discussion: The sample
showed low levels of waxes with water content between 2,99 and 4,83%
and 0,67-1,33% for ash content. The scan data revealed the maximum
absorption at 425 nm, corresponding to the region of absorption of total
flavonoids. The validation results showed to be linear in the concentration
range 0.004-0.012-1 mg.mL. The limit of detection and quantitation were
0.00188 and 0.00572 mg.mL-1 respectively. The average content of total
flavonoids presented by red propolis from Alagoas was 9.68%.
Conclusions: Results obtained in the pre-validation showed that the
method meets the requirements of accuracy and linearity, demonstrating
reliability required for an analytical method, therefore, considers the
method validated.



44

CODE 43
COMPARISON OF METHODS FOR DETERMINATION OF CONTENT
PROTEIN IN THE AQUEOUS AND ACETONE EXTRACT Annona
squamosa

Pinto, Y. S.
1
; Campos, R. S.
1
; Arajo, N. K
2
; Pagnoncelli, M. G. B.
1
; Assis,
C. F.
1

1
Laboratrio de Bromatologia, Departamento de Farmcia, Universidade
Federal do Rio Grande do Norte.
2
Laboratrio de Engenharia Bioqumica,
Departamento de Engenharia Qumica, Universidade Federal do Rio
Grande do Norte
Corresponding author: yohana_farmaciaufrn@hotmail.com

Introduction: Spectrophotometric determination of total protein is used in
several areas such as clinical analysis, food science and technology,
biochemistry, protein chemistry, physiology. The Annona squamosa is
native to tropical America and produces edible fruit that originate medicinal
products. The fruits usually contain proteins essential for the body. Thus,
quantification is essential for the insertion of the fruit in the diet. Objective:
The aim of this work was to quantify the content protein (aqueous and
acetone extracts) using spectrophotometric methods. Materials and
Methods: Annona squamosa were obtained from supermarket, in
Prudente de Moraes Avenue, Rio Grande do Norte, Northest in Brazil, and
stored at 5C. Then, aqueous and acetone extracts were made from the
peel and seed. The three methods were used: BCA, Lowry and Biureto
methods. Results and Discussion: The acetone showed greater
efficiency in the extraction of proteins than water, due to ability of acetone
precipitate proteins. Then the acetone extracts showed higher values of the
total content protein. According to the methods used, BCA method was
more sensitive than Lowry and Biureto. The content of protein in pell (30g
protein/100g pell) was higher than seed (1,2 g protein/100 g seed).
Conclusion: The higher amount of protein was found in pell and the more
sensitive methods for determination protein were BCA, showing that pell in
foods presented important nutrients in human diet.

Financial support from REUNI/Brazil


45

CODE 44
PHYSICO-CHEMICAL PARAMETERS OF LANETTE
AND POLAWAX

EMULSIONS FOR MANOEL CASADO DE ALMEIDA/UFCG, PB
PHARMACY SCHOOL PRODUCTION

Silva, A. M. S.
1,3
; Souza, J. B. P.
2,3
; Alencar, J. S.
2,3

1
Farmcia Escola Manoel Casado de Almeida, Faculdade de Farmcia,
Universidade Federal de Campina Grande (UFCG).
Corresponding author: alaine.maria@hotmail.com

Introduction: The Pharmacy School, a UFCG supplementary organ, will
serve as a training field for pharmacy students, providing medicines and
cosmetics handled to Serid and Curimata Paraiban regions. Therefore,
the physico-chemical parameters it is important to the development and
achievement products to be handled. For emulsions production was used
ionic and nonionic waxes such as Polawax and Lanette, respectively. It
is important to know the product behavior to ensure its physico-chemical
and sensory characteristics. Objective: To evaluate creams and lotions
Lanette and Polawax waxes based physico-chemical characteristics for
Manuel Casado de Amed Pharmacy School / UFCG, PB production.
Material and Methods: The formulation used was Lanette 5% and 10%
and Polawax 10% and 15% for lotion and cream, respectively. For the
physico-chemical analysis were performed centrifugation, spreadability,
viscosity, electrical conductivity, microscopic analysis and pH tests, and
macroscopic homogeneity aspects, odor and color. Results and
Discussion: The lotions Lanette
and Polowax
pH were within the

standard range (between 5.5 and 7.0), however, the creams showed a
slightly lower pH. In Spin, only the lotion Lanette
showed two phases, it

was the only formulation with little uniformity under microscopic vision.
Lanette

emulsions showed greater spread and higher electrical
conductivity. Conclusion: The research showed the importance of
physico-chemical evaluation of emulsions, demonstrating that some
parameters should be optimized as the pH of creams and the Lanette
lotion concentration should be adjusted to achieve greater stability, as well
as improve macroscopic characteristics.


46

CODE 45
MICROBIOLOGICAL ANALYSIS OF WATER FROM DIFFERENT
SOURCES OF FORTALEZA IN THE PERIOD JANUARY TO
SEPTEMBER 2011.

Assis, C. N.,*, Th, M. F. D. S.; Costa, M. A. R.; Carvalho, M. M. F.,
Oliveira, Y. S.; Bezerra, R. G.; Souza, F. M. B.

Department of Pharmacy, Federal University of Cear, Fortaleza/CE-
Brazil
Corresponding author:clarissa.nunes.cna@gmail.com

Introduction: The water is currently one of the major global concerns
regarding its bacteriological quality. The decree N 518 of March of 2004
of Ministry of Health establishes the control and surveillance of water
quality for human consumption and its potability standards. Therefore, it
becomes extremely important to search for bacterial indicators of water
quality in order to avoid any problems caused by ingestion or direct contact
with contaminated water. Objective: Evaluate the presence of Total and
Fecal Coliforms and P.aeruginosa in water intended for human
consumption. Materials and Methods: We analyzed 136 water samples in
the period January-September 2011 by chromogenic substrate method for
the investigation of Total and Fecal coliforms and Seeding Technique in
Cetrimide Agar for the investigation of P.aeruginosa. Results and
Discussion: Among the samples analyzed, 26.47% were reproved due to
the presence of Total Coliforms and 37,78% of these had Fecal Coliforms.
Of the samples analyzed, the highest incidence occurred in samples from
wells in the region (38.89%). This index indicates that the water that does
not undergo previous treatment should not be used by local people. It was
detected the presence of P.aeruginosa in one sample. According to the
legislation, the presence of these microorganisms evidence that the water
is unfit for human consumption. Conclusion: A considerable percentage of
water consumed in the local evaluated presented contamination by Total
and Fecal Coliforms. Therefore, the authorities should attempt to carry out
measures to improve quality of the water commonly used in Fortaleza.


47

CODE 46
UTILIZATION OF Mg, Al LAYERED DOUBLE HYDROXIDES (LDHs) AS
A CARRIER OF DIGOXINE FOR VECTORIZATION IN CHEMOTERAPY
OF CANCER

Santos, L. D. A.
1
; Barbosa, V. T.
1
; Grillo, L. A. M.
2
; Magalhes, N. S. S.
3
;
Dornelas, C. B.
1

1
Laboratrio de Tecnologia e Controle de Medicamentos (LabTCoM),
Curso de Farmcia, Escola de Enfermagem e Farmcia, Universidade
Federal de Alagoas UFAL.
2
Laboratrio de Biocincias Farmacuticas
(LabBFar), Curso de Farmcia, Escola de Enfermagem e Farmcia,
Universidade Federal de Alagoas UFAL.
3
Laboratrio de Imunopatologia
Keiko Asami (LIKA), Universidade Federal de Pernambuco UFPE.
Corresponding author: lislliedayse@hotmail.com

Introduction: The search for alternatives in anti-tumor therapy involves the
achievement of target-specific drugs for greater efficacy and fewer side
effects. Digoxin is a very established drug in cardiovascular therapy, but
recent reports also indicate its success in clinical use in chemotherapy of
prostate and breast cancer. It will be the drug-test in a LDH nanocarrier
system, working with folate, present specifically in tumor cells cited.
Objective: This work presents the results obtained from the first step, the
synthesis of LDH. Material and Methods: The LDHs will be characterized
by: XRD, porosity, TG, FTIR and TEM in a collaboration of UFPE. Results
and Discussion: The first two tests are already concluded and the rest are
ongoing. The values of interplanar distances (d) were calculated and the
main Miller indices from the corresponding plane to each obtained value of
d were identified. They were similar to those assigned to hydrotalcite in the
mineralogical manuals. d of the plane (003) can fluctuate between 7,6-7,8,
depending on the hydration degree, a value of 7,86 was obtained. The
values of 4,48m/g for the specific superficial area, 14,80nm for the
diameter and 0,012cm/g for the volume of pores were determined by the N
2

adsorption isotherm.The analysis of the diffractogram suggests the
formation of Mg, AI LDH; the porosity test provides greater details of the
obtained material. Only with the other methods of characterization it will be
possible to assert the success of the synthesis. This step is fundamental for
antimonial-LDH nanocomposites development.

Financial support: CNPq and FAPEAL/Brazil.


48

CODE 47
CREATION OF DIGITAL'S MAGAZINES ON RATIONAL USE OF
DRUGS: NEW PERSPECTIVES ON EDUCATION.

Silva, L. C.
1
; Cardoso, E. T. C.
1
; Silva, A. D. V.
1
; Pinheiro, A. M.
1
; Pontes,
A. C. A. de A.
1
; Souza; S. C. C.
1
; Campos, D. M.
1
; Luz, D. A.
1
; Silva, J. V.
S.
1
;

Silva, M. L.
1
; Silva, M. C. M.
1
; Bastos, M. L. C.
1
; Sarmento
1
, R. M.;
Carvalho, M. N.
3
; Dolabela, M. F.
2

1
Integrante do Grupo PET Farmcia da Universidade Federal do Par
(UFPA).
2
Farmacutica, Professora do Curso de Farmcia da UFPA, Tutora do
PET-Farmcia.
3
Farmacutica colaboradora CEAF. Grupo PET-Farmcia (UFPA).
Corresponding author: petfarmacia_ufpa@yahoo.com.br

Introduction: Accodng o Wod Heh Ognzon, A on use
exists when patients receive medications appropriate to their clinical
needs, in doses that meet their own individual requirements, for an
adequate period of time, and at the lowest cost to them and their
communy. Educve con whch nvoves he opc of on use hs
contributed for reduce of the irrational use of medicaments. Objective: The
group PET- FARMCIA-UFPA is building a website containing a series of
characters and environments, with the purpose of raise population
consciousness about dangers of incorrect use of medicines. Results and
Discussion: The site is in construction stages where members of the
group are elaborating the stories and creating the characters as the
pharmacist, the hipocondriaco, among other. The program for creation of
vectors Corel Draw X5 is being used to create the character and after will
be used the program Adobe Flash Player 11.0.1.15 to create animation. All
will be available to population though a site with periodic update. In this
way, it is intended, by means of interactivity, stimulate the rational use of
medicines.


49

CODE 48
CHRONIC, BUT NOT ACUTE, ETHANOL WITHDRAWL AFTER
CHRONIC CONSUMPTION PROMOTES DEPRESSIVE-LIKE
BEHAVIOUR WITHOUT ALTERING LOCOMOTION IN RATS.

Ali, M. S.
1
; Sousa Pinto, I. A.
1
; Andr, E.
1
; Padovan, C. M.
2
; Gavioli, E. C.
1
;
Soares-Rachetti, V. P.
1

1
Depto. de Biofsica e Farmacologia-CB/UFRN,
2
Depto. de Psicobiologia
FFCLRP/USP.
Corresponding author:vanpaso@cb.ufrn.br

Introduction: Chronic abuse of ethanol can generate depression-related
symptoms [J Consult Clin Psychol. 78(3):362-74, 2010]. Such symptoms
persist over time and contribute to relapse [Alcohol Health Res World
22(1):61-6, 1998]. Objective: Observe if the withdrawal of ethanol after
chronic consumption generates depressive-like behavior on forced
swimming test (FST) or alters locomotor activity in rats. Materials and
Methods: Male Wistar rats were subjected to chronic consumption of
increasing concentrations of ethanol (2%, 4% and 6%), for 21 days and the
control group received water ad libitum. In day 21, ethanol was replaced by
water (withdrawn) and 3, 4 and 5 days (experiment 1) and 20, 21 and 22
days (experiment 2) were submitted to open field, pre-test and FST,
respectively. FST is based on animal exposure to a cylinder (30cm
diameter) with water (25 cm in height) at 24 1 C for 15 and 5 min in the
pre-test and test session respectively. Results and Discussion: Data
showed h ehno whdw ddn e he ocomoo cvy of
animals in open-field test for both experiments. In relation to immobility
time in the FST, the ethanol group (experiment 1) showed no difference
from the control group. In experiment 2, the ethanol group showed
increased immobility time when compared to control [mean standard
error of average time (in seconds); Control: 117.43 17.30; Ethanol:
190.56 10.70, n = 7-9].Conclusion: Ethanol withdrawal after chronic
intake leads depressive-like behavior after chronic withdrawal but not
acute. Further studies are required and can be useful to propose new
therapeutic strategies in addiction.

Financial support: PROPESQ-UFRN.


50

CODE 49.1
APPLICATION OF THERMOANALYTICAL METHODS IN EVALUATING
THE BEHAVIOR OF TUBERCULOSTATICS DRUGS PRODUCED BY
NUPLAM / UFRN: ISONIAZID AND PYRAZINAMIDE

Gurgel, R. K. C.
1
; Valena, R. C. A.
1
; Moura, M. F. V.
1

1
Laboratrio de Qumica Analtica e Meio Ambiente, Departamento de
Qumica, Universidade Federal do Rio Grande do Norte (UFRN).
Corresponding author: rayannekaren@hotmail.com

Introduction: Some medicines are marketed in combination, such as the
tuberculostatics. Therefore, it is necessary to use useful tools such as
thermoanalytical methods to assess the compatibility among drugs that are
in binary mixtures. Objective: To evaluate the thermal behavior and the
possible interactions of the binary mixture isoniazid / pyrazinamide, which
is in different proportions, through the interpretation of TG, DTG and DTA
curves. Materials and Methods: DTG-60 system from Shimadzu was
used. It consists of one differential thermal analyzer coupled to a thermal
balance for obtainment of measurements, and the TA60 software from the
equipment manufacturer, for the treatment of the data. Results and
Discussion: Through analysis of the data obtained from the TG curves, it
was observed that the thermal decomposition of pyrazinamide alone
occurred in two stages, with mass loss. To isoniazid alone, the event
occurred in a single stage, given that pyrazinamide had become more
stable, with a difference of almost 50.0 C. The curves of differential
thermal analysis, however, showed a melting point in a temperature lower
hn sonzds. Fo mxues obseved, he meng pons wee owe
than those of the individual drugs, the TG curve showed decomposition
behavior profiles at higher temperatures than those observed for
pyrazinamide, and lower than those observed for isoniazid. Conclusions:
Bsed on he esus, s ssumed h hese dugs hs some fom of
interaction, which will be investigated in future studies involving mixtures of
these and other analytical techniques such as infrared spectroscopy.

Financial support from Propesq/Brazil and master scholarship from
REUNI/Brazil.


51

CODE 49.2
DETERMINATION OF PHYSICAL AND CHEMICAL COMPOSITION FOR
THE QUALITY CONTROL OF MILK CONSUMED IN THE CITY OF
NATAL / RN

Gurgel, R. K. C
1
; Valena, R. C. A
1
; Silva, E. I. L
1
; Carvalho, J. R. P
1
;
Pagnoncelli, M. G. B
1
; Assis, C. F
1

1
Laboratrio de Bromatologia, Departamento de Farmcia UFRN,
Universidade Federal do Rio Grande do Norte (UFRN)
Corresponding author: rayannekaren@hotmail.com

Introduction: Milk is an essential food for human health because it is
made up of proteins, carbohydrates, fats, minerals and water. Thus, its
quality control is fundamental for ensuring the health of the population.
Objective: To evaluate the quality of four samples of milk, raw and
pasteurized, by reason of minimum standards required by the Ministry of
Agriculture. Materials and Methods: The samples were chosen randomly,
one of them being from the city of Barcelona / RN. The following tests were
performed: density, acidity, determination of lipid content by Gerber
method; dry residue by the indirect method and nonfat dry residue by the
direct method; Alizarol test, test of reduction of methylene blue (TRAM)
and the peroxidase test. Results and Discussion: Two samples were
approved for human consumption. 50% were tampered with sugar, 100%
had the density according to the reference values; 100% were above the
permissible acidity; for the tests of dry and nonfat dry residue, 25% and
50%, respectively, had values above the minimum allowed; 25% did not
contain the peroxidase enzyme, since the sample was of the long life type;
for the tests TRAM and Alizarol, 50%, both tests showed normal values,
according to the reference; 100% of the samples showed lipid content
within the allowed for each type. Conclusions: In most of the milk samples
various irregularities in the physical-chemical aspects were identified,
therefore, it becomes evident the need for measures to improve the quality
of the milk consumed in the city of Natal / RN.

Financial support from UFRN/Brazil.


52

CODE 50
STUDY OF PHYSICOCHEMICAL PROPERTIES AND ACUTE TOXICITY
EVALUATION OF ETHANOLIC EXTRACT OF THE LEAVES FROM
Mikania glomerata SPRENGEL.

Santana, L. C. L. R.
1
; Brito, M. R. M.
1
; Sousa, G. F.
1
; Freitas, R. M.
1,*

1
Laboratrio de Pesquisa em Neuroqumica Experimental do Programa de
Ps-graduao em Cincias Farmacuticas, Centro de Cincias da Sade,
Universidade Federal do Piau.
Corresponding author: rivelilson@pq.cnpq.br

Introduction: The Mikania glomerata is a plant belonging to the
Asteraceae family that is widely used in folk medicine due to the actions
bronchodilator, asthma, expectorant and antitussive. Objectives: The
purpose of this study was to determine the physicochemical properties of
the powder obtained from the leaves of M. glomerata, and to evaluate the
toxicity in mice after acute treatment with repeated doses of a standardized
ethanol extract prepared from the leaves of this specie. Material and
Methods: During the study of physicochemical properties of the powder
obtained from the leaves of M. glomerata was made to determine the bulk
density and compaction, the total ash content, moisture content and
particle size, and gross morphological analysis was performed and
evaluated the acute toxicity with repeated doses on biochemical and
hematological parameters of mice. Results and Discussions: In the
physicochemical analysis, the powder flow properties presented. In studies
of acute toxicity, the therapeutic dose suggested none alterations in
parameters evaluated and found 50% lethal dose (LD50) of the extract was
much higher (~ 3000 mg kg
-1
). Conclusions: According to the results
obtained we suggest that the powder can be used in formulating a solid
dosage form that the ethanolic extract can be used safely in humans, since
it presented a value for the LD50 that can be classified as harmful, and
produced no morphological changes in major organs, biochemical and
hematological parameters of mice.

Financial support: CNPq e FAPEPI


53

CODE 51
EPIDEMIOLOGY OF CANDIDEMIA IN PUBLIC HOSPITALS OF
ALAGOAS, BRASIL

Mendona, N. M.
1
; Lima, F. S.
2
; Maranho, F. C. A.
1

1
Laboratrio de Microbiologia Geral e Clnica, Instituto de Cincias
Biolgicas e da Sade, Universidade Federal de Alagoas
(LMGC/ICBS/UFAL).
2
Centro de Patologia e Medicina Laboratorial (CPML).
Corresponding author: nayaramendonca@bol.com.br

Introdution: Species of the Candida are opportunistic pathogens most
frequently involved in superficial and mucous-cutaneous mycoses,
however is an important cause of bloodstream infections. Candidemias
rates vary geographically like a systemic disease associated with high
mortality in nosocomial ambient. Objective: Evaluation of Candida spp.
incidence in blood samples obtained in the major public hospitals of
Macei, Alagoas (Brasil). Materials and Methods: During the period
studied (April to December 2010) we analyzed the frequency of Candida
spp in patients treated in three public hospitals of Macei. Candida species
were identified through standard microbiological methods in suspect blood
samples by use of Sabouraud Agar (24- 48 h a 37C), Gram stain in
microscopy followed by cultive in CHROMagar Candida

(24- 48 h a 35C)
for presumptive identification. Results and Discussion: In a total of 2346
blood samples, 269 (11,47%) showed presence of microorganisms, being
17 identified like Candida species thought characteristics of colonies in
media and localization of blastoconidia (40x). C. tropicalis was confirmed in
35.29% (6) and C. albicans in 23.53% (4), while had 11.76% (2) of C krusei
and 29.41% of Candida sp (5), totalizing 29.41% of species non-albicans.
Conclusions: The candidemia frequency was low in the period studied,
with a prevalence of C. tropicalis followed by C. albicans. Candida non-
albicans in fungemia are reemergents during nosocomial infections and the
adequate identification is important to guide therapeutic choices, since
there is resistance specie-related and the emergence of azole-resistant C.
glabrata and C. krusei is an actual problem in hospitals.


54

CODE 52.1
VALIDATION OF ANALYTHICAL METHODOLOGY FOR Passiflora
incarnata L. EXTRACT BY HPLC AND ANALYISIS OF COMMERCIALS
EXTRACTS FROM RS.

Wildner, A. C.
1
; Meneghini, L. Z.
1
; Hexsel, H. T.
1
; Gnoatto, S. C. B.
1
;
Bergold, A. M.
1

1
Laboratrio de Qumica Farmacutica, Faculdade de Farmcia, UFRGS

Introduction: The extracts of Passiflora incarnata L. have become very
important for the therapeutic of mind diseases. This species is considered
as an official plant by WHO, ANVISA and many official codes such
European Pharmacopoeia (2002) and French Pharmacopoeia (2007).
However, the Farm. Bras. V, 2010, does not support information about this
species. Therefore, the present study intents to develop an analytical
method to assay of P. incarnata L. extracts appropriate for quality control.
Objective: The objective of this study is optimize the HPLC method for
analysis of vitexin, a C-glycosil flavonoid, in Passiflora incarnata L. extract;
also evaluate the content of commercial extracts available in Rio Grande
do Sul. Materials and Methods: The HPLC analyses were performed
using a Shimadzu chromatograph, an isocratic system, C-18 column and
security guard cartridges. Results and Discussion: For the qualitative
analysis the retention times and UV spectra of samples were compared
with those of reference standards. The method had validated and applied
to quantitative analysis of five commercial formulations of RS.
Conclusions: The experimental results proved that the proposed HPLC
method is selective, precise, accurate, robust and therefore suitable for
detection, separation and quantification of flavonoid constituents and
derivatives related to vitexin, present in extractive samples. This method
was also employed to analyze commercial samples. Of the seven P.
incarnata L. excs nyzed 40% ddn hd vexn, vd ndco of
authenticity, and 60% had levels between 0.008% a 0. 031% of vitexin.

CAPES/Brazil.


55

CODE 52.2
DEVELOPMENT AND VALIDATION OF A METHOD OF DISSOLUTION
FOR DARIFENACIN EXTENDED-RELEASE TABLETS BASED ON IN
VIVO DATA

Meneghini, L. Z.
1
; Wildner, A.C.
1
; Junqueira, C. A.
1
; Adams, A. I. H.
2
;
Frehlich, P. E.
1
; Bergold, A. M.
1

1
Programa de Ps-Graduao em Cincias Farmacuticas, Faculdade de
Farmcia, Universidade Federal do Rio Grande do Sul-UFRGS. Porto
Alegre, RS, Brasil.
2
Universidade Federal de Santa Maria - UFSM, Centro de Cincias da
Sade, Depto. Farmcia Industrial, Santa Maria, RS, Brasil.

Introduction: Several antimuscarinic drugs are on the market like
darifenacin, chemically (S)-2-{1-[2-(2,3-dihydrobenzofuran-5-yl)ethyl]-3-
pyrrolidinyl}-2,2- diphenylacetamide hydrobromide. It is a potent muscarinic
receptor antagonist, available as a hydrobromide salt in Enablex, an
extended-release tablet which contains 7.5 mg or 15 mg of the drug.
However, there is not pharmacopoeial method for dissolution test.
Objective: The aim of this work was to develop and to validate a
dissolution test for the Enablex extended-release tablet based on in vivo
data according to current FDA and USP guidelines. Materials and
Methods: The sink condition was evaluated and a factorial design 24-1
was employed for method development. In vivo data were obtained from
the literature and fraction (and percentage) of dose absorbed (FA) was
calculated using Wagner- Nelson method.
Results and Discussion: The best dissolution conditions were achieved
using a USP apparatus I (basket), with 900 mL of dissolution medium
containing 2% SDS at 50 rpm. A successful linear regression model model
of fraction of drug absorbed versus dissolved was achieved (R2=0.9997,
p<0.05). The validation was performed with an LC/UV method and results
for specificity, linearity, precision and accuracy were in accordance with
guidelines.Conclusions: The proposed method allowed achieving a level
A correlation with the fraction of dose absorbed. The results showed that
the test is adequate to evaluate the in vitro profile for extended-release
darifenacin tablets.

CAPES/Brazil.


56

CODE 53.1
Lobophora variegata AND ITS ACTIVITY IN PAW EDEMA AND
HEPATOTOXICITY IN RATS

Will, L. S. E. P.
1
; Castro, A. J. G.
1
; Nascimento, M. S.
1
; Magalhes, J. E.
M.
1
; Pinheiro, T. S.
1
; Almeida, H. W. B.
1
; Costa, T. G.
1
; Medeiros, H. N.
1
;
Leite, E. L.
1

1
Laboratrio de Glicobiologia Vegetal, Departamento de Bioqumica,
UFRN, Rio Grande do Norte, Brazil.
Corresponding author: lsepwill@gmail.com

Introduction: Seaweeds or marine algae have been reported to contain
many important compounds which act as antibiotics, laxatives,
anticoagulants, anti-ulcer products and suspending agents in radiological
preparations. The algae have also several pharmacological activities such
as anticoagulant, anti-inflammatory, anti-edematous, antitumor and
antioxidant activities. Objective: The aim of this study was to investigate
the effect of sulfated polysaccharides (F0.8) from Lobophora variegata
seaweed on some models of inflammation. Material and Methods: The
paw edema and hepatotoxicity were assessment in Wistar rats. Paw
edema was used to demonstrate the effect of fucan in the carrageenan-
induced inflammation. For hepatotoxicity assay was used as induced of
hepatic toxicity the carbon tetrachloride (CCI4). Results and Discussion:
An protective anti-edematous effect of F0.8 decreasing of paw thickness at
25 mg/kg occur inhibition dose- dependent in 89.110.0%, 50 mg/kg
inhibited in 91.17.1 % and 75 mg/kg inhibited in 100.01.4% the edema in
the fourth hour of the experiment, demonstrating to be dose-dependent.
The hepatoprotective effect observed showed an effective decrease (p <
0.001) in serum levels of indirect bilirubin (80.002%, 160.0008% and
640.0005%). Hepatics enzymes (AST, ALT, and y-Glutamyl Transferase)
in plasm were measured and the best hepatoprotective efects was showed
in the concentration 75 mg by animal body-weight (p < 0.05). Histological
analysis with H&E confirmed the hepatoprotective effect of this polymer.
Conclusion: The results lead us to conclude that the sulfated fucans of
F0.8v have significant anti-inflammatory and hepatoprotective activities.

Supported by: CNPq and CAPES.


57

CODE 53.2
-GLUCANS OF CARIPIA MONTAGNEI MUSHROOM SHOWS ANTI-
INFLAMMATORY ACTIVITY: NEWS PERSPECTIVE OF PPAR ATION

Will, L. S. E. P.
1
; Castro, A. J. G.
1
; Nascimento, M. S.
1
; Pinheiro, T. S.
1
;
Magalhes, J. E. M.
1
; Almeida, H. W. B.
1
; Medeiros, H. N.
1
; Alves, M. G. C.
F.
1
; Filgueira, L. G. A.
1
; Leite, E. L.
1

1
Corresponding author: lsepwill@gmail.com

Introduction: The receptors activated
by peroxisome proliferators (PPAR) are the class of linked
receptors with the regulation of inflammation. Was known that
glucans act as anti-inflammatory influencing directly the phagocytic
activity of immunomodulatory cells. Therefore, was verified the
action of B Glucans extracted from the Caripia montagnei
mushroom in inflammation PPAR way. Material and Methods: Cell
viability of fibroblasts was analyzed by MTT, incubated with B-
glucans and perfluorooctanoic acid (PFOA). The anti-inflammatory
activity of polysaccharides, ligands of PPAR (GW9662, and
ciprofibrate PFOA Rosiglitazone) Parecoxib, indomethacin and L-
NAME was assessed by the pleurisy model and paw edema induced
by carrageenan, being measured leucotaxis rate, nitric oxide
levels and inhibition of paw edema. Also evaluated the synergy
between the polysaccharides tested with the PPAR ligands,
AINEs and L-NAME. Results and Discussion: Was observed a cell
viability rate of 91.6 4.5% when incubated for 24 h with B-
glucan (50 g/m) and of 87.9 0.3% when incubated with B-
glucan + PFOA. The glucans (90 mg/kg) inhibited in 81.03 07.03%
the leucotaxis and in 47.31% the release of nitric oxide,
compared with the control, the edema in 90.7 .5% in the
fourth hour while indomethacin, Parecoxib, L-NAME and PFOA
decreased by 92.4 .6%, 47.4% 1.7%, 78.5 4.3%
and 90.8 10.9%, respectively. L-NAME, Parecoxib and PFOA had
synergy with B-Glucans tested. Other PPAR ligands
tested inhibited in, or less than, 50%. Conclusion: Thus was
verified an effective anti-inflammatory activity of B-glucans
of Caripia montagnei, being this possibly PPAR way.



58

CODE 54
EVALUATION OF HYPOTENSIVE RESPONSES OF LYOPHILIZED
AYAHUASCA TEA IN SHR RATS

Moura, M. T. D.
1
; Herculano, E. A.
1
; Costa, C. D. F.
1
; Oliveira, M. P.
1
;
Netto, S. M.
2
; Ribeiro, . A. N.
1

1
Laboratrio de Farmacologia Cardiovascular, Escola de Enfermagem e
Farmcia,UFAL, Macei, AL, Brasil.
2
Departamento de Psicobiologia,
UNIFESP, So Paulo, Brasil.
Corresponding author: moura.mtd@hotmail.com

INTRODUCTION: Ayahuasca tea is a psychoactive drink of South
American origin, used in indigenous rituals and the Amazonian region of
Brazil certain religions. It is prepared by decoction of the vine
Banisteriopsis sp ("mariri") with the leaves of the shrub Psychotria sp
("chacrona"), and species Banisteriopsis caapi and Psychotria viridis the
most frequently used. In previous studies our group found hypotensive and
vasorelaxant activity of tea ayahuasca in SHR rats (Herculano et al, 2011).
OBJECTIVE: The aim of this study was is to elucidate the possible
mechanism of action involved in hypotensive effects of lyophilized
ayahuasca tea (LYT) in SHR rats, by in vivo approach. MATERIALS AND
METHODS: For the measurement of blood pressure and heart rate, male
spontaneously hypertensive rats (250-350 g) were anesthetized with
thiopental (45 mg / kg, ip) and polyethylene catheters were inserted into
the abdominal aorta and inferior vena cava for recording of pressure and
drug administration, respectively. The same procedure was used to verify
the participation of nitric oxide (NO) in the hypotensive effect by blocking
the enzyme NO synthase. For this we used a blocker of NO synthase
pathway, NG-nitro-L-arginine methyl ester (L-NAME, 20 mg / kg, iv). The
experiments were performed 24 hours after surgery. The results are
presented as mean standard error of mean. The statistical analysis used
the "t" Student test with a significance of p < 0,05. RESULTS AND
DISCUSSION: Intravenous administration of LYT (0.5, 1, 5, 10, 20, 30, 50,
and 60 mg / kg, randomly) induced hypotension in doses of 20, 30, 50 and
60 mg / kg (-3, 1.5, -7.1 0.8, -5.8 2.0, -2.4 1.3, -8.2 0.6, - 18.4
0.4; -9.5 1.2, -22.8 1.1 mmHg, respectively) and a slight bradycardia at
doses of 0.5, 1, 5 mg / kg more pronounced at a dose of 60 mg / kg and
tachycardia in doses of 10, 20, 30 and 50 mg / kg (-5.8 0.5, -3.8 1.1, -
3.0 0.8, 6.3 1.7, 4, 4 1.3, 11.8 1.4, 13.0 1.0 and -35.9 0.3 bpm,
respectively. After administration of NG-nitro-L-arginine methyl ester (L-
NAME, 20 mg / kg, iv), the hypotensive response to LYT was significantly
increased and heart rate was significantly changed, after nitric oxide (NO)
synthase blockade (L-NAME, 20 mg/Kg, i.v.). CONCLUSIONS: These
results demonstrate that tea promotes hypotensive effect in SHR rats. The
L-NAME was also capable of significantly changing LYT-induced effects in
rats SHR, suggesting that NO appears be participating of this effect.

Sources of research support: FAPEAL, CAPES and CNPq.


59

CODE 55.1
ANGIOGENESIS INHIBITION AND B-GLUCANS ANTITUMOR ACTION
OF CARIPIA MONTAGNEI MUSHROOM

Nascimento, M.S.
1
, Castro, A.J.G.
1
, Will, L.S.E.P.
1
, Almeida, H.W.B.
1
,
Florentein, K.Q.
1
, Paiva, A.A.O.
1
, Costa, T.G.
1
, Leite, E.L.
1

1
Universidade Federal do Rio Grande do Norte (UFRN), Rio Grande do
Norte, Brazil.
Corresponding author: maricbbio@yahoo.com.br

Introduction: B-glucans are homopolymers of D-glucose which have
bioactivity, such anti-inflammatory, antioxidant and antitumor properties,
being the latter promoted by one possible antiangiogenesis action, which
develop a critical function in the metastasis and growth of solid tumors.
Objective: Therefore aimed to analyze the antiangiogenic and antirumor
properties of B-glucans from Caripia montagnei mushroom. Material and
Methods: Its antitumor action was analyzed by the MTT method, where
the HeLa cell line were incubated at the time 24 and 48 hours in presence
or absence of glucans at different concentrations (25, 50 e 100 g/ml).
Was also used the chorioallantoic membrane method (MCA) to evaluate
the antiangiogenic activity. Chicken eggs were incubate in a greenhouse,
after access to MCA the glucans were administered about this, being
counted the number of vessels formed after 24 hours and semi-quantified
by score system. Heparin and spironolactone were used as positive and
negative controls, respectively. Results and Discussion: Was observed
that the cytotoxicity rate for HeLa cells incubated in 24 hours period with C.
montagnei polysaccharides was 83.4 2.3 % e de 94.36 2.3 % for 48
hours period (100 g). The antiangiogenic activity showed that the 1000
g/egg concenon of gucns pesened sgnfcn ngogeness
inhibition rate, compared the heparin and spironolactone action.
Conclusion: Thus, was noted that the C. montagnei B-glucans showed
significant antiangiogenic and antitumor activities, which proposed this
mushroom as a potencial drug.



60

CODE 55.2
CARIPIA MONTAGNEI MUSHROOM B-GLUCANS SHOWING NON
HEMOLYTIC ACTION AND ANTIOXIDANT ACTIVITY: POTENTIAL
FUNCTION IN LIPIDIC PEROXIDATION INHIBIT AND OXIDATIVE
STRESS

Nascimento, M.S.
1
, Castro, A.J.G.
1
, Will, L.S.E.P.
1
, Magalhes, J.E.M.
1
,
Dore, C.M.P.G.
1
, Almeida, H.W.B.
1
, Baseia, I.G.
2
, Alves, L.G.F.
1
, Leite,
E.L.
1

1
Universidade Federal do Rio Grande do Norte (UFRN), Rio Grande do
Norte, Brazil.
2
Laboratrio de Micologia, Departamento de Botnica, Ecologia e
Zoologia, Universidade Federal do Rio Grande do Norte (UFRN), Rio
Grande do Norte, Brazil.
Corresponding author: maricbbio@yahoo.com.br

Introduction: The oxidative stress was caused by imbalance between the
reactive oxygen species producion and antioxidant compounds, being
favorable to free radicals liberation which can let to cellular aging,
cardiovascular diseases and cancer, trigerred by among others, for lipid
peroxidation which is consequent of oxidative actions in plasmatic
membrane. Thus, compounds that can act like antioxidants had notorious
physiological and medical importance. Objective: Therefore, aimed to
analyze the antioxidant and non hemolytic properties of B-glucans
extracted from Caripia montangnei mushroom. Material and Methods:
The inhibition of Hydroxyl radicals action, Total antioxidant activity,
Reducing power, Lipidic peroxidation, Activity of ferric ion chelation and
Hemolytic activity of B-glucans in different concentrations were analyzed
by spectrophotometry. Results and Discussion: Was verified significant
antioxidant activity of the polysaccharides tested. 1 mg/ml concentration of
B-glucans extracted from Caripia montangnei inhibits in 37.9 0.01% the
hydroxyl radicals formation, showing total antioxidant activity of 47.1% and
reducing power of 50% with 4 mg/ml concentration, which compared withal
ascorbic acid standard activity. With the same concentration observed
reduction in 37.60.16% in the lipidic peroxidation process and ferric ion
chelation rate of 95 0.002%. Was also verified that all tested
concenons (25, 50 nd 100 g/mL) showed no significant hemolytic,
with the highest concentration showing 8.40.008% hemolysis, compared
to positive control (Triton-X). Conclusion: Thus, was verified a significant
antioxidant and non-hemolytic activity of glucans of the C. montagnei
mushroom.



61

CODE 56
DETERMINATION OF MOISTURE AND LIPIDS IN FRUITS OF Licania
tomentosa Beth (Oiti)

Teixeira, L. L.; Amaral, N. K. M.; Macedo, J. B. M.; Santos, E. C. G.;
Oliveira, S. D. S.; Moura, M. F. V..

Programao de Ps graduao em Qumica PPGQ
Programa de Ps graduao em Cincias Farmacuticas PPGCF

Materials and Methods: Samples of the fruits of Licania tomentosa Beth
(Oiti) were collected in February 2011 in the city of Natal - RN. Fruits were
collected in relatively mature stage of ripening, a total of 226 fruits. The
fruits were divided into peel, pulp and seed. Determination of moisture
content is executed by the gravimetric method and for determination of
lipids is used the Soxhlet extraction method. Results and Discussion: As
a result we found that the average content of moisture to the peel was 60.5
g (+ / -1.6) in 100 g of sample, 72.3 g (+ / -0.9) in 100 g of pulp and 45.7 g
(+ / -2.0) moisture in 100 g of seed. For the results of lipid was obtained 3.7
g (+ / - 0.09) of lipids in 100 g of peel, 0.4 g (+ / - 0.03) 100 g of lipids in the
pulp and 0, 05 g (+ / -0.004) of lipids in 100 g of seed. Conclusions:
Through these results we conclude that the peel is part of the plant with the
highest level of lipids.


62

CODE 57
EVALUATION OF THE CARDIOVASCULAR EFFECTS OF Syzigium
cumini SEEDS

Herculano, E. A.; Feitoza, P. R
.
; Nascimento, W. U. C.; Costa, C. D. F.;
Aquino, P. G. V.; Frana, P. H. B.; Arajo-Jnior, J. X.; Sant'Ana, A. E.
G.; Ribeiro, . A. N.
Escola de Enfermagem e Farmcia, UFAL, Macei/AL,
Instituto de Qumica e Biotecnologia, UFAL, Macei/AL.

Introduction: Syzigium cumini (Myrtaceae) is a plant popularly known as
bnco-de-vv or jmeo. The spece hs been descbed n he
literature as antifungal (J Antimicrob Chemother. 60; 312, 2007), anti-
allergic (Braz J Med Biol Res 40; 1, 2007), anti-diabetic (Planta Med. 9;
139, 1986), and anti-mutagenic (Phytochemistry. 36: 1027, 1994).
Objective: To investigate the acute cardiovascular effects of the ethanolic
extract of the Syzigium cumini seeds (EESCS) in rats. Materials and
Methods: The extract was prepared by the percolation method with 95%
ethanol, followed by steam evaporation. Exactly 250 g of dry powder was
percolated to get a net yield of 30.6 g of concentrated extract (12.24%).
Male Wistar and SHR rats (250-350g total weight) were anesthetized with
sodium thiopental (45mg/kg, i.p.). Polyethylene catheters were inserted
into the lower abdominal aorta and into the inferior vena cava, for blood
pressure measurements and administration of drugs, respectively. After a
24h period, experiments were performed in conscious unrestrained rats.
The results are presented as meanstandard error of the mean. The study
was approved by the ethics committee of the Federal University of Alagoas
(010151/2008-82). Results and Discussion: The EESCS (0.5; 1; 5; 10;
20 and 30 mg.kg
-1
i.v., randomly, n=5) in Wistar rats elicited immediate and
dose-independent decreases in mean arterial pressure (MAP) (-6.2
3.0%; -4.5 0.1%; -10.4 2.1%; -9.3 0.6%; -7.6 1.2%; -11.8 4.2%,
respectively) with low reduction in heart rate (HR) (-3.1 4.2% ; -1.9
1.9%; -1.5 0.4%; -3.1 0.9%; -5.5 3.3%; -1.1 1.1%, respectively). In
SHR the effects hypotensive (-3.2 1.0%; -3.9 1.0%; -5.1 0.9%; -3.8
1.0%; -5.4 1.1%; -6.9 2.2%, respectively) and bradicardic were also
observed (1.6 0.9%; 0.6 0.7%; 2.7 1.2%; -2.0 0.7%; -4.2 1.1%; -
4.4 2.0%, respectively), however, the bradicardic effect just in higher
doses 10; 20 and 30 mg/kg, only). Conclusions: These results suggest
that the extract is able hypotensive actions with reduction in heart rate at all
doses in normotensive rats and just higher doses in hypertensive rats
probably due to a genetic differences between animals.

Sources of research support: FAPEAL, CAPES and CNPq.


63

CODE 58.1
POLYSACCHARIDES EFFECTS FROM LOBOPHORA VARIEGATA IN
A TOPICAL INFLAMMATORY MODEL

Pinheiro, T.S.; Nascimento, M.S.; Magalhes, J.E.M.; Alves, M.G.C.F.;
Castro, A.J.G.; Paiva, A.A.O.; Almeida, H.W.B.; Costa, T.G.; Florentin,
K.Q.; Medeiros, H.N.; Leite, E.L.

Laboratrio de Glicobiologia Vegetal. Departamento de Bioqumica,
Corresponding author: thuanepinheiro@gmail.com

Introduction: Algae are capable to produce large variety of secondary
metabolites and are considered rich sources of structurally diverse
bioactive compounds with diverse biological activities as an anti-allergic,
antitumor and anti-inflammatory. Objective: The aim of the present
investigation was to investigate the action of sulfated polysaccharides in
contact hypersensitivity reaction induced by croton oil. Materials and
Methods: The algae passed through the collection process, spray,
delipidation and differential, proteolysis and precipitation with increasing
volumes of acetone (0.3-2.0). All fractions showed sulfated
polysaccharides. Results and Discussion: The F0.8 fraction, showed a
high yield in total weight (19.49%), low percentage of protein contamination
(0.90%) and high levels of carbohydrates (66.2%) and sulfate (3.2%).
Mouse exhibited very mild dermatitis when treated with croton oil or
oxazolone. The Contact Reaction is an induction of irritating oil in local
edema with topical treatment in ear mouse. Most animal models used are
based on skin inflammation induced inflammatory responses in acute skin
by application of agents such as croton oil. The animals ears were
measured and histological analysis were made. It was found in treated
groups with different concentrations the reduce swelling occurred in a
dose-dependent. Dermal response was evaluated by H&E and
immunohistochemistry. This initial study suggests the 0.8 fraction of algae
L.variegata has anti-inflammatory and anti-allergic activities. Conclusions:
These results suggest and are support, for the hypothesis that contact
dermatitis is a classic delayed type hypersensitivity, and demonstrate the
importance of the interaction between the irritant and algal that the irritant
effect of chemicals may be mediated through the cutaneous innate immune
system.

Supported by CNPq and CAPES.


64

CODE 58.2
ANTIOXIDANT ASSAYS OF BRUTE EXTRACTED FROM
PLECTTRANTUS BARBATUS ANDR.

Pinheiro, T.S.; Magalhes, J.E.M.; Will, L.S.E.P.; Castro, A.J.G.; Paiva,
A.A.O.; Dore, C.M.P.G.; Costa, T.G.; Medeiros, H.N.; Filgueira,
L.G.A.; Leite, E.L.

Laboratrio de Glicobiologia Vegetal. Departamento de Bioqumica,
Corresponding author: thuanepinheiro@gmail.com

Introdcution: Latest studies showing that oxidative stress was caused by
disbalance between the reactive species of oxygen production and
antioxidant compounds, and this is favorable to free radicals liberation.
Thus, compounds that can acting like antioxidants had notorious
physiological and medical importance. Objective: This study aimed
analyze the antioxidant properties of the brute extracted from Plectranthus
barbatus Andr. (Boldo Brasileiro). Materials and Methods: The brute
extracted used was obtained by gradual fractionation with acetone, and the
polysaccharides contained in this extracted were used in the antioxidant
assays. The sequestration of DPPH radical, Superoxide radicals, Hydroxyl
radical and inhibition of lipidic peroxidation in different concentrations were
analyzed by spectrophotometry at 517 nm, 560 nm, 510 nm and 532 nm
respectively. Results and Discussion: It was verified one significant
antioxidant activity of the polysaccharides tested, which with 2 mg/ml
concentration inhibits in 89.96% of the reaction involving DPPH radical.
With the same concentration can inhibit 22.0% of superoxide radicals, and
the inhibition of hydroxyl radicals formation was 75% in the same
concentration. Lastly this concentration can inhibits 15% the lipidic
peroxidation and all tests were compared with ascorbic acid standard
activity. Conclusions: Thus, was verified a significant antioxidant activity
of polysaccharides from Plectranthus barbatus.

Supported by CNPq and CAPES.


65

CODE 59
EVALUATION OF DENTIN ROUGHNESS AFTER USE OF DIFFERENTS
CHELATING AGENTS EMPLOYED IN ENDODONTICS

Tartari, T.
1
; Almeida, B.V.N.
1
; Silva Jnior, J.O.C.
2
; Duarte Jnior, A.P.
2
;
Negro, C
3
; Costa, C.E.F
3
; Pessoa, O.F.
1
; Silva e Souza, P.A.R
1
.

1
Laboratrio de Materiais Dentrios, Faculdade de Odontologia,
2
Laboratrio de P&D Farmacutico e Cosmtico, Faculdade de Farmcia,
3
Laboratrio de Catlise e Oleoqumica, Faculdade de Qumica,
Corresponding author: carrera@ufpa.br

Introduction: The chelating agents used during endodontic treatment to
promote the cleaning of dentinal walls may cause changes in surface
roughness increasing the micromechanical bonding of root canal sealers.
Objective: Evaluate the effect of different irrigation schemes employing
chelating agents on dentin roughness. Materials and Methods: Fifty root
fragments of human teeth were randomly divided, according to irrigation
schemes, into five groups: G1- saline (physical control) for 30 minutes; G2-
2,5% sodium hypochlorite (NaOCl) for 30 minutes; G3- 2,5% NaOCl for 30
minutes followed by 17% ethylenediaminetetraacetic acid (EDTA) for 3
minutes; G4- 2.5% NaOCl for 30 minutes followed by 10% citric acid for 3
minutes; and G5-NaOCl 5% associated with 18% etidronate solution
(HEBP) for 30 minutes. To compare the results, measurements of dentin
roughness were made with a roughness tester before and after treatment
with irrigating solutions. Results and discussion: Analysis of the results
by T-Test showed that in G1 (p=0,29) and G2 (p=34) there were no
changes in surface roughness. The groups G3 (p <0.0001), G4 (p <0.0001)
and G5 (p <0.0001) had significant increase in roughness, being
numerically superior in G5. The group using saline showed that irrigators
do not exercise physical effect on the roughness, but this was significantly
increased as a result of chemical action of chelating agents. The NaOCl
did not alter the roughness, except when associated with HEBP,
suggesting that NaOCl may potentiate the effect of soft chelators.
Conclusion: Irrigation schemes which employing chelating agents cause
significantly increase in dentin surface roughness.


66

CODE 61
TOPICAL APPLICATION OF PROPOLIS FOR HEALING OF WOUNDS
SURGICALLY INDUCED AND EVALUATION OF BIOCHEMICAL
PARAMETERS IN WISTAR RATS.

Costa, M. M
1
; Nascimento, J. S.
1
; Da Costa, Y. G. F
1
; Menezes, J. B.
1
;
Bastos, M. L. A.
3
; Batista, L. L. V.
2
; Dornelas, C. B
2
; Grillo, L. A. M.
1

1
Laboratrio de Bioqumica e Biologia Molecular;
2
Laboratrio de
Tecnologia e Controle de Medicamentos (LabTCoM);
3
Laboratrio de
Tratamento de Feridas;
1,2,3
Escola de Enfermagem e Farmcia, Universidade Federal de Alagoas,
Brasil.
Corresponding author: maricosta_90@hotmail.com

Introduction: Propolis is considered a prominent natural product, known
by its diverse biological properties. The action of propolis in tissue
regeneration and granulation is currently suggested as a product that
favors wound healing in addition to its natural antibiotic property, free from
side effects and its low cost in comparison with other products currently
applied on wounds. Nevertheless, little is known about of its use as healer
of wounds, systemic absorption and alterations in the biochemical
parameters. Objective: The scope of this study was to evaluate alterations
on the biochemical parameters caused by treatment with the propolis for
the wound healing. Materials and Methods: The methodology consisted
of treatment of wounds in Wistar rats randomly divided into three groups of
five animals: control (saline solution), topical application of the green and
red propolis extract for 15 days. After this period the animals were
sacrificed and the blood was used for we evaluated of the biochemical
parameters. Results: It was found that of green and red propolis extract
significantly decreased of triglycerides, glucose and cholesterol levels
against control group. Conclusions: These results indicated that propolis
may have roles in the carbohydrates and lipids metabolism. Other
parameters are being evaluated, including, aminotransferases, urea,
albumin and uric acid.

Supported by: Fapeal


67

CODE 62
EVALUATION CYTOTOXIC IN VITRO OF NEW COMPOUND LPSF/MS-
02

Carvalho,

M. S.
1
; Pinto,

M. N. S.
2
*; Lima, M. C. A.
3
; Galdino, S. L.
3
; Pitta, I.
R.
3
; Pessoa, C. .
4
; Moraes, M. O.
4

Departamento de Biofsica e Farmacologia; Centro de Biocincias,
UFRN. Av. Salgado Filho, s/n Campus Natal, RN
Departamento de Farmcia da UFRN, R. Gal. Gustavo Cordeiro de
Farias, S/N Petrpolis. Natal/RN
Departamento de Antibiticos da UFPE, Av. Prof. Moraes rego, Cidade
Universitria,1235, Recife, PE
4
Laboratrio de Oncologia Experimental da UFC, Rua Coronel Nunes de
Melo, 1127, Fortaleza, CE
Corresponding author: manuelacarvalho@cb.ufrn.br

Key-words: Hydantoins, imidazolidine, cytotoxicity

Introduction: The chemistry and properties of hydantoins and their
derivatives have been investigated for more than 140years. The hydantoin
moiety represents an important pharmacophore, which is present in
various biologically active compounds. Objectives: The compound
LPSF/MS-02 [3-(4-methyl-benzyl)-5-(4-chloro-benzylidene)-imidazolidine-
2,4-dione was synthesized and tested on the human cancer cell lines: HL-
60 (promyelocytic leukemia), MDAMB-435 (melanoma - human), HCT-8
(colon - human) and SF-295 (glioblastoma - human). Materials and
Methods: For comparative purposes we used an anticancer drug used in
the clinic, doxorubicin (Dox). The IC
50
values were calculated by exposure
of the compounds tested in human tumor cell lines after 72 h of incubation,
the MTT test. The compounds were serially diluted in RPMI to obtain the
final concentrations (0,19-25 g/mL) and added in 96-well plates
(100L/poo). Results and Discussion: The plates were read on
spectrophotometer plate at a wavelength of 595nm. Active were
considered those that showed IC
50
< 4 g/mL. LPSF/MS-02 was more
selective for the melanoma cell line (MDAMB-435) with IC
50
equal to 0.63
g/mL, with a cytotoxic dose very close to the standard dose of the
anticancer doxorubicin (0,48 g/mL). Statistical analysis was performed
according to their means and their standard errors and their deviations
were made from non-linear regression using GraphPad Prism (version 5).
Conclusions: Compound LPSF/MS-02 was synthesized in satisfactory
yield and characterized spectroscopically and showed cytotoxic potential
on human cell lines, which showed selectivity for lineage MDAMB-435, with
a cytotoxic dose very close to the standard dose of the anticancer
doxorubicin (0,48 g/mL).


68

CODE 63
DETERMINATION OF PROTEIN CONTENT OF Zizyphus joazeiro.

Campos, R.S.
1
; Pinto, Y.S.
1
; Arajo, N.K.
2
; Pagnoncelli, M.G.B.
1
; Assis,
C.F.
1

1
Laboratrio de Bromatologia, Departamento de Farmcia, Universidade
Federal do Rio Grande do Norte
2
Laboratrio de Engenharia Bioqumica, Departamento de Engenharia
Qumica, Universidade Federal do Rio Grande do Norte

Introduction: The Zizyphus joazeiro is widely distributed throughout the
Brasilian Northest, its leaves and fruits are valuable food source for
humans and animals. Objectives: The aim of this work was evaluate two
excs (ehnoc nd queous) usng dffeen mehods of poens
determination. Materials and Methods: Ziziphus joazeiro fruits were
collected in Macaba city at Escola Agrcola de Jundia region of Rio
Gnde do Noe Se, Bz, ude 927S nd ongude 3527W of
Greenwich, between the months of May and June, 2011.Than, were made
aqueous and ethanol pulp and seed for protein quantify by
spectroptotometer and content of nitrogen. The three methods
spectroptotometer were used: BCA, Lowry and Biureto methods and
Kjeldahl determination. Results and Discussion: The content of protein
using the aqueous extract was higher than ethanolic extract; this confirms
the higher solubility of proteins in water than organic solvent. According to
the spectrophometric methods used in this work the most sensitive was
BCA (Bicinchoninic Acid) methods. The pulp presented 12,5g protein/100 g
sample, while seed 3,02 g protein /100 g sample. The Kjeldahl method
present results of 6,66 g protein/100 pulp and 2,91 g protein/100 g seed. In
comparison to the absolute values determined by Kjeldahl method, BCA
remained the most reliable protein assay when quantifying protein of seed
and pulp. Conclusion: According to our results we can conclude that the
pulp has more protein and that the BCA had a higher sensibility in
comparision of the others methods.


69

CODE 64
COMPARISON OF LABORATORY CHARACTERISTICS OF PATIENTS
WITH SICKLE CELL DISEASE

Fernandes, T.A.A.M.
1,5
; Bezerra, C.M.
2
; Cabral, C.H.K.
3
; Leito, R.L.T.S.
3
;
Nascimento, A.N.C.
4
; Lopes, D.R.
4
; Sonati, M.F.
5
; Medeiros, T.M.D.
4

1
Departamento de Cincias Biomdicas, Universidade do Estado do Rio
Grande do Norte (UERN)

2
Departamento de Microbiologia e Parasitologia, Universidade Federal do
Rio Grande do Norte (UFRN)

3
Hemocentro Dalton Barbosa Cunha (HEMONORTE)

4
Departamento de Anlises Clnicas e Toxicolgicas, Universidade
Federal do Rio Grande do Norte (UFRN)

5
Laboratrio de Micologia, Natal-RN

6
Departamento de Patologia Clnica, Universidade de Campinas
(UNICAMP)
Corresponding author: thalesallyrio@yahoo.com.br

Introduction: Sickle cell disease (SCD) is a constellation of similar
disorders, all of which have at least 50% HbS in the blood. Homozygotes
for the HbS gene are said to have sickle cell anemia; common compound
heterozygous forms of disease include HbSC disease and HbS-|
thalassemia. The phenotype are highly variable among theses patients.
Objective: Compare the laboratory characteristics of patients with different
genotypes of sickle cell disease. Materials and methods: We analyzed 44
patients (25 SS, 12 SC and 7 S-|) attended at HEMONORTE, Natal-RN.
The patients had not received blood transfusions in the preceding 3
months nor had taken hydroxyurea during the last 12 months. They were
stratified by age group and we analyzed the following laboratorial
parameters: erythrogram (RBC, HGB, HCT, MCV, MCH, MCHC, RDW),
reticulocyte count, Hb A2 and Hb Fetal, leukocyte count, bilirubin (total,
direct and indirect), creatinine, lactate dehydrogenase (LDH), aspartate
aminotransferase (AST), alanine aminotransferase (ALT) and ferritin.
Results and Discussion: The levels of RBC, HGB and HCT were
significantly lower in patients SS, and VCM and HCM were lower in S-|
patients. Besides, we found higher levels of reticulocyte count, Hb F and
LDH in patients SS, when compared to SC. These results can be attributed
to the higher levels of hemolysis found in patients SS, and the higher level
of HbF can be explained by our exclusion criteria. Conclusions: Patients
with different genotypes of sickle cell disease present statistically
significant differences in some hematological and biochemical parameters.

Financial Support: CNPq / Brazil, UERN / Brazil


70

CODE 65.1
UV SPECTROPHOTOMETRIC PROFILE OF CEFAZOLIN SODIUM

Pedroso, T. M.
1*
; Salgado H.R.N.
1

1
Departamento de Frmacos e Medicamentos - Controle de Qualidade,
FCF, UNESP, Araraquara, SP, Brasil
Corresponding author: tahisa@fcfar.unesp.br

Introduction: Cefazolin sodium (CFZ) is an antimicrobial agent -lactam
parenteral drug belonging to first generation cephalosporins. Its use in
clinical practice stands out for its effectiveness and therapeutic agent as in
surgical prophylaxis. Objective: This work proposes quantitative tests for
the inclusion of cefazolin sodium in the Brazilian Pharmacopoeia. Material
and Method: Spectrophotometer UV Shimadzu, quartz cells, aqueous
solutions of CFZ at concentrations of 8, 12, 16, 20, 24 and 28 g/mL. A
standard calibration curve of CFZ was constructed by plotting absorbance
versus concentration. The dilutions were analyzed individually in the UV
and using their respective absorbance values an absorption curve was
constructed. Results and Discussion: In the spectrophotometer, the
absorption was obtained at 271 nm. The linearity of the method was
proven by the correlation coefficients that were 0.9999 for standard
reference and sample. The content of CFZ in the samples analyzed,
98.777%, is consistent with the official compendium from 95.0 to 102.0%.
The accuracy of the method was proven by the recovery test, with an
average of 100.56%. The robustness was assessed at 2 wavelengths,
above and below 271 nm. Conclusions: The CFZ showed a linear relation
between absorbance and concentration in wavelength. Thus, the results
parameters demonstrated that the spectrophotometric method could be
applied for the analysis of the pharmaceutical formulations assuring the
quality and efficacy of the CFZ under investigation.

Financial support: FAPESP, CAPES, PADC-FCF and ABL
Pharmaceutical industry.


71

CODE 65.2
A NEW, SAFE AND EASY METHOD OF THIN-LAYER
CHROMATOGRAPHY FOR CEFAZOLIN SODIUM IDENTIFICATION.

Pedroso, T. M.
1*
; Salgado H.R.N.
1

1
Departamento de Frmacos e Medicamentos - Controle de Qualidade,
FCF, UNESP, Araraquara, SP, Brasil
Corresponding author: tahisa@fcfar.unesp.br

Introduction: Cefazolin sodium is an antimicrobial agent -lactam
parenteral drug belonging to first generation cephalosporins. The Thin-
Layer Chromatography (TLC) is a common method fo dugs denfcon,
because it is simple, fast, visual and relatively inexpensive. TLC is based
on differential migration of components over a thin layer of adsorbent
material retained on a flat surface. Objective: This research aims to
develop an easy, safe and economical TLC method for cefazolin sodium
identification, which can be used in the routine analysis of quality control.
Material and Method: The mobile phases tested in this work were:
methanol; absolute ethyl alcohol: water (80:20, v/v). The stationary phase
used was silica gel impregnated in aluminum. The standard and sample
solutions of cefazolin sodium were prepared in purified water at
concentration of 10 mg/mL (w/v). It was applied 5 L of both solutions on
the plate. After the mobile phses euon, he spos wee deeced by
exposing the plate at UV chamber (365 nm) and/or iodine vapor until they
appear. Results and Discussion: The chromatogram showed clearly two
separated spots. The spot in the chromatogram obtained with the test
solution was similar in position, color and size to the spot in the
chromatogram obtained with reference solution. Conclusions: The TLC
method proposed in this study is easy, safe for the operator, economical
and can be used for cefazolin sodium identification, in the routine analysis
of quality control.


72

CODE 66
VOLTAGE-OPERATED POTASSIUM CHANNELS ARE INVOLVED IN (-
)-BORNEOL-INDUCED VASORELAXANT RESPONSE ON RAT
MESENTERIC ARTERY

Silva-Filho, J. C.
1
; Oliveira, N. N. P. M.
1
; Ferreira-Filho, E. S.
1
; Sabino, C.
K. B.
1
; Mendes, M. B.
1
; Arcanjo, D. D. R.
1
; Quintans-Junior, L. J.
2
; Santos,
M. R. V.
2
; Oliveira, R. C. M.
1
; Oliveira, A. P.
1

1
Ncleo de Pesquisas em Plantas Medicinais, Universidade Federal do
Piau (UFPI).
2
Departamento de Fisiologia, Universidade Federal de Sergipe (UFS).
Corresponding author: aldeidia@gmail.com

Introduction: The monoterpene (-)-borneol is found in essential oils of
several medicinal plants. Previously, (-)-borneol showed analgesic, anti-
inflammatory, antioxidant, vasorelaxant and anti-hypertensive properties.
Objective: Evaluate the potassium channels involvement in (-)-borneol-
induced vasorelaxant effect on rat superior mesenteric artery. Materials
and Methods: Endothelium-intact and denuded superior mesenteric
artery rings (1-3 mm) from male Wistar rats were kept in Tyrode (pH 7.4;
37C), aerated with 95% O
2
and 5% CO
2
suspended by cotton threads and
attached to force transducers coupled to a data acquisition system (AVS
Projetos, Brazil) for registration of isometric tension. After a stabilization
(1h / 0.75 g), rings were contracted with phenylephrine (10
-5
M) and (-)-
borneol (10
-9
- 10
-3
M) was added on contraction tonic phase. Potassium
channels involvement were evaluated by tetraethylammonium (3 mM), 4-
aminopyridine (1 mM) or glibenclamide (10 M) pretreatment. Values were
expressed as mean S.E.M. and results were considered significant when
*p < 0,05. Results: The cumulative administration of (-)-borneol (10
-9
- 10
-3
M) induced a concentration-dependent vasorelaxant effect and
independent of vascular endothelium (pD
2
= 4.86 0.18; 4.92 0.14,
endothelium-intact and -denuded, respectively; n=5). In endothelium-
denuded rings, (-)-borneol-induced vasorelaxant response was attenuated
after tetraethylammonium (pD
2
= 4.38 0.10*) or 4-aminopyridine (pD
2
=
4.41 0.08*) pretreatment, but not in glibenclamide pretreatment (pD
2
=
4.86 0.12). Conclusion: The monoterpene (-)-Borneol promoted a
endothelium-independent vasorelaxant effect on rat superior mesenteric
artery, and voltage-operated potassium channels are probably underlying
this effect.

Financial support from UFPI, UFS and CAPES/Brazil.


73

CODE 67
PREPARATION OF NANOSTRUCTURED SYSTEMS SUNSCREENS
ORGANIC INORGANIC

Rodrigues, K.
1,2
; Silva, T. A.
3
; Grillo, L. A. M.
4
; Dornelas, C. B.
3
;
Magalhes, N. S.
1,2

1
Departamento de Farmcia, Universidade Federal de Pernambuco.
2
Laboratrio de Imunopatologia Keizo-Asami (LIKA), Bioqumica,
Universidade Federal de Pernambuco Universidade Federal de
Pernambuco.
3
Laboratrio de Tecnologia e Controle de Medicamentos, Escola de
Enfermagem e Farmcia Curso de Farmcia, Universidade Federal de
Alagoas (UFAL).
4
Laboratrio de Biocincias Farmacuticas, Escola de Enfermagem e
Farmcia Curso de Farmcia, Universidade Federal de Alagoas (UFAL).
Corresponding author: katyyrodrigues@hotmail.com

Introduction: The improvement of research on products that block the
various effects of ultraviolet radiation incident on the human body became
the use of sunscreen indispensable. Several studies have demonstrated
the necessity of better understanding new and traditional molecules used
as sunscreens, as the use of some organic sunscreens can cause
undesirable reactions such as photoisomerization and photosensitization,
with a reduction of action, and potential systemic absorption. To overcome
these limitations, it is necessary to develop safe and effective sunscreen
preparations. Objective: The aim of this work was to prepare
nanostructured systems sunscreens containing an organic sunscreen
liquid-oil and an inorganic filter with lamellar silicate. Materials and
Methods: The nanosystems were prepared by the solvent method, and the
suspension obtained was submitted centrifugation and evaporation at
reduced pressure to remove the solvent. Results and Discussions: After
the rotaevaporation the nanosystems maintained the oily appearance but
those obtained by centrifugation presented powder aspect. The result
suggests that organic filter was intercalated between the silicate lamellae.
Through spectrophotometry was possible to indirectly quantify the yield of
reaction products obtained by centrifugation, which reached 95.36
1.58%, significant result compared to others nanosystems that can carry
drugs. Conclusions: As perspective, it is expected to analyze the possibly
reduction of the adverse effects of the organic sunscreen, supposedly
achieved by the formation of intercalated type structure, as well as ratify
the synergism of the filters on the increase in sun protection factor.

CAPES/Brazil.


74

CODE 68
ANTIBACTERIAL EVALUATION OF NEW DERIVATIVES OF
THIOPHENE

Xavier, L. A.
1
; Arajo, G. M.
1
; Medeiros, R. G. L.
1
; Pinto, A. C. S.
3
;
Mendona Junior, F. J. B.
4
; Lima, M. C. A.
4
; Galdino, S. L.
4
; Pitta, I. R.
4
;
Moura, R. O.
5
; Carvalho,

M. S.
2
; Andrade, V. S.
1
rade

1
Laboratrio de Micologia Mdica e Ambiental (LAMEA), Departamento de
Microbiologia e Parasitologia, Centro de Biocincias, Universidade Federal
do Rio Grande do Norte (UFRN).
Laboratrio de Farmacologia, Departamento de Biofsica e Farmacologia,
Centro de Biocincias, Universidade Federal do Rio Grande do Norte
(UFRN).
Curso de Farmcia, Centro de Cincias da Sade, Universidade Federal
4
Laboratrio de Planejamento e Sntese de Frmacos, Departamento de
Antibiticos da Universidade Federal de Pernambuco (UFPE).
5
Laboratrio de Sntese e Vetorizao de Molculas, CCBSA,
Universidade Estadual da Paraba (UEPB).
Corresponding author: vsandrade@cb.ufrn.br

Introduction: One structural characteristic present in several biologically
active compounds, is the presence of the thiophene ring. Compounds with
this feature present biological properties such as anti-inflammatory,
antiviral and antifungal activities, making this a very attractive structure for
medicinal chemists. Given the importance of the thiophene ring indicated in
the literature, we pursued new thiophene derivative bioactive compounds
like potential antibacterial activity. Objective: Evaluate the antibacterial
potential in vitro thiazolidine analogs structurally modified. Materials and
Methods: Filter paper discs were impregnated with samples (1mg/mL) and
distributed on the surface of Mueller-Hinton agar previously inoculated with
bacteria suspension. The strains tested were Staphylococcus aureus
ATCC 25923 and Escherichia coli ATCC 25922, were positives controls,
respectively, oxacillin and norfloxacin (incubated at 36C for 24 hours). The
determination of minimum inhibitory concentration was performed from the
broth microdilution using gentamicin (10g/mL) as positive control and for
both tests: distilled water, 5% DMSO and 5% tween as a negative control.
Results and Discussion: Among the series two compounds showed
activity against a strain of S. aureus. The formation of small inhibitory zone
observed in disk diffusion test and the color displayed in the development
of microdilution suggest a possible bacteriostatic action, to be confirmed in
bioassays currently in progress. These compounds deserve further
structural investigations that may contribute to the enhancement of
antimicrobial activity, considering the limitation of adaptive mechanisms of
resistance by bacteria and their cytotoxicity. Conclusions: The constant
search for new bioactive compounds is the first step in the development of
new drugs as an alternative to the treatment of infections caused by
resistant microorganisms.

Financial support from CAPES, UFRN (PROPESQ-REUNI) /Brazil.


75

CODE 69
THEORETICAL AND PRACTICAL HANDBOOK: A TOOL FOR THE
TRAINEESHIP LIVE PHARMACIES.

Moura, C. A.
1
; Bandeira, M. A. M.
1

1
Corresponding author: camila_9626@hotmail.com

Introduction: Setting the list of medicinal plants to be used in Herbal
Medicine in Public Health, The State Board of Phytotherapy, based on the
UFC Living Pharmacies/LP Project, took into account the epidemiological
profile of the population of Cear and the efficacy of treatment, then thirty
medicinal plants selected were scientifically validated. The graduation
students of the Course of Pharmacy UFC perform curricular traineeship on
the LP that as one of the focus identifying these plants included in that
Project, so for these students recognize these medicinal plants in herbal
medicine services are required research sources reliable and easily
accessible. Objective: Develop a Theoretical and Practical Handbook
about characterization of medicinal plants to assist in traineeship in LP.
Materials and Methods: We carried out several literature researches on
methods of macro and microscopic identification of the component parts of
plants. From these data, we prepared a Handbook adapted to the thirty
medicinal plants consisting of on the theoretical characterization of leaves,
bark and underground organs. Compounding the album yet, put up boards
with illustrative drawings and tables that enable gather therapeutic
information, botanical identification and major macro and microscopic
features of these parts, with room for schematic drawings by the trainees.
Results and Discussion:Using the Handbook, during the traineeship is
expected the consolidation of knowledge acquired by trainees.
Conclusions:The development of this Handbook, in addition to improving
the fixation of the knowledge acquired during the traineeship, can be used
by students as a source of research on plants of LP.


76

CODE 70
DEVELOPMENT OF STRATEGIES OF MOLECULAR DIAGNOSTIC
APPLIED TO DYSLIPIDEMIA

Osorio, E. D. S. S.; Santos, A. B.; Bastos, R. C.; Andre, N. V. C.;
Azevedo, N. L.; Rezende, A. A.; Luchessi, A. D.; Silbiger, V. N.

Laboratrio de Biologia Molecular, Departamento de Anlises Clnicas e
Corresponding author: ikes.alu@hotmail.com

This study select candidate genes and SNPs to establish molecular
strategies to genotyping and gene expression associated with
cardiovascular diseases (CDs) and dyslipidemia. The selection candidate
genes were performed by research in articles published (2008-2011) in
PubMed/NCBI followed by gene and diseases interaction analysis using
the Ingenuity Pathway Analysis. Candidate SNPs were select using the
dbSNP/NCBI. The primers for genotyping strategies were determined
using the Primer Express 3.0 to the TaqMan system, Primer
BLAST/NCBI and Web Site NEBcutter V2.0 to the PCR-RFLP and
PyroMark Assay 2.0 to the pyrosequencing of according with this priority
order. Firstly 35 were selection as candidate genes of dyslipidemia. Among
of them 14, 4 and 5 genes presented direct, indirect and without
relationship with dyslipidemia, respectively. Considering the previously
association with CDs at the end 11 genes were selected, 6 to elaborate the
genotyping strategies (CHI3L1; FABP2; INSIG1; NPC1L1; PCSK9 and
TRIB1) and another 5 genes for gene expression (APOA1; APOB; APOC3;
APOE and LDLR). It was possible to select 4 SNPs of gene CHI3L1, 8 of
FABP2, 10 of INSIG1, 6 of NPC1L1, 1 of PCSK9 and 10 of TRIB1. Fifty-
two percent of SNPs can be determinate by TaqMan real time PCR, 2%
by PCR-RFLP and 23% by pyrosequencing. Also it was possible select
primers and probes of gene expression by TaqMan real time PCR. This
study created one list of candidate genes, SNPs and mRNAs using tools of
interaction between genes and diseases providing the determination of
strategies of genotyping and gene expression.


77

CODE 71
EVALUATION OF THE ROLE OF THE NITRIC OXIDE IN
GASTROPROTECTIVE ACTIVITY OF PARTITION FRACTIONS OF
Neoglaziovia variegata Mez. (BROMELIACEAE) IN MODEL OF
ULCERS INDUCED BY ETHANOL

Machado, F. D. F.
1
; Bezerra, M. A.
1
; Diniz, J. A.
1
; Viana, A. F. S. C.
1
;
Oliveira, I. S.
1
; Lima, J. T.
2
; Almeida, J. R. G. S.
2
; Oliveira, F. A.
1
; Oliveira
R. C. M.
1

1
Ncleo de Pesquisas em Plantas Medicinais, Universidade Federal do
Piau (UFPI).
2
Colegiado de Cincias Farmacuticas, Universidade Federal do Vale do
So Francisco (UNIVASF).
Corresponding author: menesesoliveira@gmail.com

Introduction: Previous studies have shown that partition fractions of
ethanol extract of Neoglaziovia variegata Mez (NV-EtOH), presents
cytoprotective activity in animal models of ulcer induced by ethanol and
ischemia/reperfusion.Objective: To evaluate the involvement of nitric oxide
(NO) in the gastroprotection of ethyl-acetate (NV-AcOEt), hexane (NV-Hex)
and chloroform (NV-CHCl
3
) fractions in models of gastric ulcer induced by
ethanol in mice. Materials and Methods: Swiss albino mice (25-30 g, n =
10), fasting 18 h, were treated intraperitoneally with vehicle (0.9 %
saline/10 mL/kg) or L-NG-nitro arginina (L-NOARG 70 mg/kg). After 30
min, the animals received NV-AcOEt, NV-Hex, NV-CHCl
3
at dose 100
mg/kg p.o, vehicle (0.9 % saline p.o) or L-arginina (600 mg/kg i.p.).
1 h after treatment with NV-AcOEt, NV-Hex, NV-CHCl
3
or vehicle and 30
min later administration of L-arginina each animal was given orally 0.2 ml
of ethanol. It were euthanized 30 min later. After euthanasia all the
stomachs removed, opened and area of injury measured by planimetery
(mm
2
). All animal experiments protocols were approved by Ethics
Committee of the Federal University of Piau (N 076/10). The significance
level was evaluated for values of *p < 0.05 (ANOVA one way). Results
and discussion: L-NOARG reversed the protection gastric
mucosa presented by NV-AcOEt (1.8 0.25 *) to (15.9 3.02), NV-Hex
(3.5 0.32 ***) to (23.86 3.16), however not inhibited the gastroprotector
effect of NV-CHCl
3
significantly. Conclusions: The results suggesting a
possible participation of NO in the gastrprotective activity NV-AcOEt and
NV-Hex.

Financial support: UFPI, CAPES, UNIVASF, FACEPE, CNPq/Brazil.


78

CODE 72
OPTIMIZATION OF AN EXTRACTION PROCEDURE FOR THE
BIOPOLYMER CHITIN

De Souza, I.
1
; Santos, S. R. A.
1
; Pedrosa, M. F. F.
1,2
; Fonseca, J. L.
3
; Silva-
Jnior, A. A.
1,2

1
Undergraduate course of Pharmacy, Federal University of Rio Grande do
Norte (UFRN).
2
Graduate Program in Pharmaceutical Sciences, Federal University of Rio
Grande do Norte (UFRN).
3
Department of Chemistry, Federal University of Rio Grande do Norte
(UFRN).

Introduction: The biggest activity of shrimp farming in Brazil is located in
Rio Grande do Norte. The waste from this production is generally
discharged. However it is possible to extract chitin, a polymer widely used
as raw material for adsorbents, chelating agents and for production of
chitosan. Objectives: To optimize an extraction procedure for the
biopolymer chitin using the shell waste. Materials and methods: The
extraction procedure was conducted in laboratory scale and optimized by
varying the concentration of HCl (2.5% w/ v, 5.0% w/ v, 7.5% w/v 10%
w/v). The physicochemical characterization included thermal analysis
(thermogravimetry - TG), Infrared Spectroscopy (IR), scanning electron
microscopy (SEM) and X-ray diffraction (XRD).Results and discussion:
The TG analysis showed that chitins treated with different acid solutions
presented different mineral residues (22.49, 16.73, 10.19 and 12.99%) for
samples treated with 2.5, 5.0, 7.5 and 10.0% respectively. The IR spectrum
showed the characteristic stretching bonds at 3444cm-1 (OH axial
stretching OH); 3270cm-1 e 3107cm-1 (NH axial stretching)of chitin.
The X-ray analysis confirms the crystallinity of the compound extracted.
SEM micrographs chitin showed homogeneous, compact and smooth
surface particles.Conclusion: The experimental results confirmed that the
smpe exced ws -chitin. XRD patterns showed the characteristic
crystalline domains. TG curves showed that the mineral residues
decreased with increasing solvent concentration, and the better acid
solution was with HCl 7.5% w/v. The optimization of extraction procedure
was effective showing that variations in the solvent concentration can
interfere with the purity of this biopolymer.


79

CODE 73.1
USE OF POTENTIALLY INAPPROPRIATE MEDICATIONS AND THE
RISK OF POSSIBLE DRUG INTERACTIONS BETWEEN
INSTITUTIONALIZED FRAIL ELDERLY IN A CITY IN THE ZONA DA
MATA MINEIRA

Zimmermann-Franco, D. C.
1,2
; Fochat, R. C.
1,2
; Horsth, R. B. O.
1
; Sette, M.
S.
3
; Raposo, N. R. B.
1,2
; Chicourel, E. L.
1,2

1
Ncleo de Identificao e Quantificao Analtica, Faculdade de
Farmcia, Universidade Federal de Juiz de Fora (UFJF).
2
Ncleo de Pesquisa e Inovao em Cincias da Sade, Faculdade de
3
Faculdade de Medicina, Universidade Federal de Juiz de Fora (UFJF).
Corresponding author: courel@terra.com.br

Introduction: Drugs have great importance in health care for the elderly
and are more consumed with advancing age. However, some drugs are
considered potentially unsuitable for the elderly because they are not
effective and/or are an unnecessarily high risk. The use of multiple
medications also should be supervised because it increases the risk of
drug-related problems. Objectives: To evaluate the presence of potentially
inappropriate medications and possible drug interactions between
institutionalized frail elderly in Juiz de Fora (MG). Materials and Methods:
It was consulted personal records and prescriptions of 122 individuals. The
drugs were classified according to Anatomical Therapeutic Chemical
Classification System and the Beer-Fick criterion to identify those
potentially inadequate. Possible interactions were established with
Micromedex. Results and Discussion: The results showed that 68.0% of
the subjects were women, 55.7% were 80 years or older and 67.2% of
them were polymedicated. Diseases of the circulatory system (27.0%)
were the most prevalent. There were 219 possible interactions (87.7%
severity moderate/severe and 70.8% of scientific evidence good/excellent)
involving 75 elderly (61.5%), and the antiepileptics, psycholeptics,
diuretics, agents that act in renin-angiotensin system were the main
responsible for possible interactions. It was observed that 44 elderly
(36.0%) used 58 potentially inappropriate drugs (8.2%), 87.9% could have
serious implications.
Conclusion: These findings indicate some gaps in prescription and
highlight the need to review the drug regimens, aiming towards their
rational, safe and effective use.

Financial support: CNPq; CAPES; Propesq/UFJF.


80

CODE 73.2
ANTIFUNGAL ACTIVITY OF OLEORESIN EXTRACTED FROM
Copaifera langsdorffii Desf AGAINST DERMATOPHYTES FUNGI

Zimmermann-Franco, D. C.
1
; Baptista, E. B.
1
; Chaves, M. G. A.
2
; Raposo,
N. R. B.
1,3

1
2
Faculdade de Odontologia, Universidade Federal de Juiz de Fora (UFJF).
3
Corresponding author: dannyzimmermann@yahoo.com.br

Introduction: Essential oils and resin stand out as the main elements
associated with the plant's defense against bacteria and fungi. Therefore, it
have been demonstrated a promising source of compounds with
antimicrobial activity. The cutaneous mycoses are diseases worldwide,
which consume millions of dollars with treatment costs each year. The
oleoresin extracted from Copaifera langsdorffii Desf. is widely used in
Brazilian popular medicine under the name copaiba, predominantly as a
healing, antiseptic and antiinflammatory agent. Objective: To evaluate the
antifungical activity of oleoresin extracted from copaiba against species of
two dermatophytes fungi: Microsporum canis and Trichophyton
mentagrophytes. Material and Methods: The qualitative antifungal assays
were performed using fragments (2 mm) of Microsporum canis (ATCC
32903) and Tricophyton mentagrophytes (ATCC 11481). The fragments
were inoculated on Sabouraud Dextrose agar previously incorporated with
oleoresin of copaiba (1000 g. mL
-1
) and the antifungal activity was
determined by total growth inhibition. The minimal inhibitory concentration
(MIC) was performed, according to recommendations of the Clinical and
Laboratory Standards Institute (M-38 A2), with adaptations. Results and
Discussion: Qualitative results showed that the copaiba oleoresin
presented activity against M. canis e T. mentagrophytes. The quantitative
evaluation showed that 125 g.mL
-1
oleoresin inhibited the growth of M.
canis and 8 g.mL
-1
inhibited the growth of T. mentagrophytes.
Conclusion: These findings provide basic information for the use of this
oleoresin extract from copaiba in fungical infectious, but further studies are
required.

Financial support: Propesq/UFJF.


81

CODE 74
EVALUATION OF ACCELERATED STABILITY OF SOYBEAN O/W
EMULSION CONTAINING ETHANOL AND BENZYL ALCOHOL AS
COSURFACTANTS

De Araujo, M. M.
1
; De Souza, I.
2
;

Streck, L.
1
; Santos, S. R. A.
2
; Oliveira,
A.G.
3
; Silva-Jnior, A. A.
1

1
Graduate Program in Pharmaceutical Sciences, Federal University of Rio
2
Undergraduate Course of Pharmacy, Federal University of Rio Grande do
Norte (UFRN).
3
Graduate Program in Pharmaceutical Sciences, Pharmaceutical Sciences
Scholl of Araraquara-SP, Unesp-SP.
Corresponding author: margaretemoreno@gmail.com

Introduction: The suitable emulsions (EMs) should present a defined
period and pre-determined of physical and chemical stability depending on
the application. Objective: This study evaluates different concentrations (5-
15%v/v) of ethanol and benzyl alcohol as cosurfactant associated with a
mixture of tween 80 and span 80 (HLB=11) in physicochemical stability of
a soybean w/o emulsions. Materials and methods: The analytical
methodology was validated with success. The stability study of different
formulations was conducted during 30 days, in different conditions every
day, such as 24 hours refrigeration (RE) (4 2 C) 24 hours ambient
temperature (AM) (25 2 C) and 24 hours warming (AQ) (45 2 C).
Different parameters such as creaming index, pH, conductivity and turbidity
of samples were recorded at intervals of 1, 7, 15 and 30 days. Results and
discussion: The ethanol did not lead to statistical differences on the
physicochemical stability parameters of the emulsions. However, the
benzyl alcohol led to decreasing the stability of the emulsions.
Conclusion: The different investigated concentrations of ethanol and
benzyl alcohol did not lead to increment in the physicochemical stability.
On the contrary, benzyl alcohol lead to unstable emulsions.

Financial support: CAPES and CNPq


82

CODE 75
EVALUATION OF MORPHOGENESIS AND PROTEINASE ACTIVITY OF
CLINICAL ISOLATES OF C. albicans OBTAINED FROM PATIENTS
WITH VULVOVAGINAL CANDIDIASIS

Medeiros, M. A. P.
1
; Melo, A. P. V.
1
; Pereira, R. M.
1
; Milan, E. P.
2
; Chaves,
G. M.
1

1
Laboratrio de Micologia Mdica e Molecular, Departamento de Anlises
Clnicas e Toxicolgicas, Universidade Federal do Rio Grande do Norte
(UFRN).
2
Departamento de Infectologia, Universidade Federal do Rio Grande do
Norte (UFRN).
Corresponding author: marianapmedeiros@hotmail.com

Introduction: C. albicans is the most virulent species of the genus
Candida. This fact can be explained by the presence of a group of
virulence attributes which contribute for its pathogenicity.
Vulvovaginal candidiasis (VVC) is one of the most common causes
of vaginitis and the majority of the cases (80 to 90%) are due to C.
albicans. Several virulence attributes are proposed in VVC, but a few
of them have been studied. Objective: The present study aims to
evaluate bud-to-hypha transition (morphogenesis) and proteinase
activity of clinical isolates of C. albicans sequentially obtained from
the anus and vagina of patients with VVC by using phenotypic
methods. Materials and Methods: We evaluated 24 strains of C.
albicans belonging to our yeast culture collection. Hypha formation
was induced by incubating Candida cells in bovine fetal serum
diluted at 20% in broth YPD, and the morphological index
determined by optical microscopy after 3 h incubation. We used the
YCB + BSA medium to induce proteinase production, and
determined the proteolytic activity as the amount of degraded
albumin as a substrate (OD 280 nm). Results and Discussion: The
morphological index of the strains ranged from 1,8 to 3,7, while the
proteinase activity ranged from 0,03 to 0,09. Conclusions: The
vaginal isolates showed in general higher virulence properties, or
similar levels to the corresponding anal isolate. While sequential
isolates showed an increase of proteinase activity followed by a
reduction of the enzyme levels, they had a trend to form filamentous
morphologies as the infection succeeded.

Financial support from FAPERN/Brazil.


83

CODE 76
DETERMINATION OF LODENAFIL CARBONATE USING CAPILLARY
ZONE ELECTROPHORESIS METHOD

Codevilla C.F.
1,*
; Ferreira P.C.L.
1
; Sangoi M.S.
1
; Frehlich P.E.
1
; Bergold
A.M.
1

1
Laboratrio de Produo de Padres Secundrios (LAPPS), Faculdade
de Farmcia, UFRGS.
E-mail: cristianefc@hotmail.com

Introduction: Lodenafil carbonate (LOC) is a phosphodiesterase type 5
inhibitors that show safety and efficacy in treating erectile dysfunction. The
literature shows few quantitation studies of LOC in tablets

and there is no
described method for determination of LOC using capillary zone
electrophoresis (CZE). Objective: The aim of this study was the
development, optimization and validation of a CZE method for the
determination of LOC in tablets, establishing comparison with the validated
HPLC and UV spectrophotometric methods. Materials and Methods: CZE
experiments were performed on an Agilent
3D
CE apparatus. All
experiments were carried out on an uncoated fused-silica capillary with 40
cm effective length, at 32.5 C. The applied voltage was 15 kV, detection at
214 nm and injection volume of 5 s at 50 mBar. The electrolyte used was
borate buffer 50 mM, pH 10. The optimized method was validated
according to ICH. Results and Discussion: The effects of background
electrolyte concentration, pH, capillary temperature and voltage were
investigated by means of response surface methodology. The method
shows to have specificity, linearity, accuracy and precision. The robustness
was studied using a 2-Level 2
4-1
fractional factorial design. Results
obtained by CZE method were compared statistically by ANOVA with
HPLC and UV spectrophotometric methods previously validated and
showed no significant difference between the techniques. Conclusions: A
simple CZE method was successfully developed for the determination of
LOC in tablets. The proposed method can be used as an alternative for the
routine quality control of LOC in pharmaceutical formulations.



84

CODE 77
ANALYSIS OF EPIDEMIOLOGICAL SITUATION OF LEPROSY IN
CHILDREN UNDER 15 YEARS OLD IN FORTALEZA IN THE PERIOD
OF 2001 TO 2010.

Costa, M. A. R.*; Oliveira, Y. S.; Th, M. F. D. S.; Carvalho, M. M. F.;
Costa, W. S.; Assis, C. N.; Amaral, H. E. G.

Department of Pharmacy, Federal University of Cear, Fortaleza/CE-
Brazil
Cell of Epidemiological Surveillance of Fortaleza (CEVEPI).
*melissacosta14@gmail.com

Introduction: Leprosy is a chronic infectious disease, transmissible, has a
slow evolution and has as etiologic agent Mycobacterium leprae. The
detection of cases in minors with less than 15 years demonstrates that
early exposure of this population to the bacillus, and has been used as an
epidemiological indicator for signaling the dynamics of recent transmission
and the magnitude of the problem. Objective: Analyze the situation of
leprosy in children under 15 years old in the city of Fortaleza in the period
2001 to 2010. Materials and Methods: A retrospective descriptive study
based on information generated by the National Notifiable Diseases
System, covering the cases of Leprosy occurred in children younger than
15 years in Fortaleza in the period 2001 to 2010. Results and
Discussion: The analysis results show a ascending behavior in the rate of
detection of the disease from 2001 to 2005. From 2006 to 2010 there was
a slight decline and in 2008 was observed a greater number of cases
reported (94). Despite this, during the whole period the number of cases
was considered high, featuring a hyperendemic (1,11/10,000 inhabitants).
It may be related to early exposure of these minors to cases not treated
yet, to the delayed diagnosis and treatment, to the incorrect diagnosis
caused by errors in the differential approach with other dermatological
diseases. Conclusion: It is extremely important the participation of health
teams in the early diagnosis of leprosy in children with less than 15 years,
with the development of preventive actions of control.


85

CODE 78.1
IN VITRO INHIBITION OF CLINICAL Pseudomonas aeruginosa
ADHESION BY XYLITOL

Sousa, L.P.
1
; Silva, A.F.
1
; Calil, N.O.
1
; Suzuki, E.Y.
1*
; Silva, S.S.
2
;
Raposo, N.R.B.
1,3

1
2
Departamento de Biotecnologia, Escola de Engenharia de Lorena
Universidade de So Paulo (USP).
3
Farmcia, Universidade Federal de Juiz de Fora (UFJF)
Author for correspondence: suzukibarbacena@uol.com.br

Introduction. In the scientific literature, there are some reports on the anti-
adherent action of xylitol, a five-carbon polyalcohol, on many bacteria. A
very promising approach is based on the awareness of the microorganisms
adherence to several surfaces, and of the mechanisms which may prevent
such colonization. Objective. The present study aimed at evaluating the
antimicrobial and the anti-adherent activities of xylitol on a clinical strain of
Pseudomonas aeruginosa, responsible for biofilms development on
implanted devices and hospital equipment. Material and Methods. The
test of antimicrobial activity was realized to determinate a minimum
inhibitory concentration (MIC), using chloramphenicol as reference
antibiotic. It was performed two parameters to evaluate the adhesion test:
1) number of colony-forming units (CFUs) released and 2) evaluation of
slides using scanning electron microscopy (SEM). Results and
Discussion. It was found that 0.5%, 2.5% and 5.0% concentrations of
xylitol do not have antimicrobial activity on the strain tested. However, the
inhibition of adherence, not dose-dependent, was demonstrated both by
counting the released bacilli and by SEM microphotographs. For 0.5%,
2.5% and 5.0% concentrations of xylitol were released, respectively,46
35 x 10
6
; 15 13 x 10
6
and 69 1 x 10
5
UFCs. Xylitol was able to prevent
the bacilli adherence on the slides evaluated by SEM, in all concentrations
tested. These results corroborate with previous studies that have shown
the use of 5.0% xylitol in the prevention of Pseudomonas aeruginosa
biofilm development. Conclusion. Xylitol could be an appropriate
biomolecule to prevent Pseudomonas aeruginosa biofilms.

Financial support from CAPES/Brazil


86

CODE 78.2
ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL FROM Baccharis
trimera (Less) DC
Suzuki, E.Y.
1*
; Chaves, M.G.A.M
2,3
; Raposo, N.R.B.
1,2

1
2
3
Faculdade de Odontologia, Universidade Federal de Juiz de Fora (UFJF)
*Author for correspondence: suzukibarbacena@uol.com.br

Introduction: Baccharis trimera (Asteraceae) is a medicinal Brazilian plant
well-known as "carqueja". Natural from tropical regions, it is traditionally
used in folk medicine as anti-inflammatory, hypoglycemiant and for the
treatment of digestive problems. Objectives. The present study aimed to
investigate the antimicrobial activity against four microorganisms and to
establish the minimum inhibitory concentration (MIC) of the essential oil
obtained from Baccharis trimera. Material and methods. The
microorganisms tested were Corynebacterium xerosis IAL105,
Staphylococcus epidermidis ATCC12228, Proteus vulgaris ATCC13315
and Micrococcus luteus ATCC7468. Antimicrobial activity was determined
using the broth microdilution method and the MIC was then established by
the turbidity in the growth medium after 24hs of incubation. The oil was
prepared at a concentration ranging from 62.5 to 1000 g/ml. In addition,
chloramphenicol (0.025 250 g/ml) was used as reference antibiotic.
Tests were carried out in triplicate. Results. Bactericidal activity of
essential oil was observed against P. vulgaris (MIC=1000g/ml) and
bacteriostatic activity was observed against M. luteus and C. xerosis with
the same MIC values (MIC=500g/ml). Chloramphenicol demonstrated
bactericidal activity against all tested microorganisms with MIC values of
25 g/ml for S. epidermidis, P. vulgaris and M. luteus and 250g/ml for C.
xerosis. It also showed bacteriostatic activity against S. epidermidis, P.
vulgaris and M. luteus with values of 2.5g/ml and 25g/ml for C. xerosis.
Conclusion. These findings provide basic information for the use of this
species in infectious processes, but further studies are required.

Financial support from CAPES/Brazil


87

CODE 80
PHYSICAL-CHEMICAL COMPATIBILITY BETWEEN ATTAPULGITE,
RIFAMPICIN AND ISONIAZID

Soares, D.S
1
, Fernandes, C. S.
1
, Raffin, F.
1
, Acchar
2
, W., Moura, T. M.
1
.

1
Drug Development Laboratory, Department of Pharmacy, Federal
University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil
2
Physical Properties of Ceramic Materials Laboratory Department of
Physics, Federal University of Rio Grande do Norte, Natal, Rio Grande do
Norte, Brazil
Corresponding author: soares_ds@yahoo.com.br

Introduction: According to composition, clays can be employed as
pharmaceutical excipients. Previous studies showed that attapulgite, one
type of clay from northeastern of Brazil, has potential for use as lubricant.
To employ a compound as excipient, it is necessary to evaluate its
compatibility with the drugs. Rifampicin and isoniazid are the main drugs
used in the treatment of tuberculosis. Objective: Evaluate the physical-
chemical compatibility between Attapulgite, Rifampicin and Isoniazid
mixtures by differential scanning calorimetry (DSC). Materials and
Methods: Physical mixtures were prepared in the different proportions of
Rifampicin, Isoniazid and Attapulgite. To evaluate physical-chemical
incompatibilities between drugs and clay, the DSC curves were obtained
on a DSC-60 cell (Shimadzu) using aluminum crucibles with about 2 mg of
sample, under dynamic N2 atmosphere (50 mL min-1) and heating rate of
10 C min-1 between 25 to 400 C. Results: On the DSC curve of
rifampicin, there is an endothermic event due to melting (Tpeak = 181,7
C). The second event is exothermic and is attributed to a recrystallization
(Tpeak = 198,7 C) and corresponds to conversion of polymorphic form II
into polymorphic form I. On the DSC curve of isoniazid, it is observed an
endothermic event due to melting (Tpeak = 172,9 C). On the DSC curve
of attapulgite, no transition it was observed. Concerning to the thermal
behavior of the mixtures comparing to the drugs alone, no significant
changes could be observed. Conclusion: According to DSC, attapulgite
presents no incompatibility with the tested drugs. Financial support:
FAPERN, UFRN.


88

CODE 81
NEW METHOD TO PRODUCE XYLAN MICROPARTICLES USING
SODIUM TRIMETAPHOSPHATE AS CROSS-LINKING AGENT

Costa, S. C.
1
; Lucena, C. A. A.
1
; Gomes, A. A. S.
1
; Marcelino, H. R.
2
;
Souza, B. S.
2
; Gomes, M. C. S.
2
; Eleamen, G. R. A.
1
; Silva, A. E.
2
; Egito,
E. S. T.
2
; Oliveira, E. E
1
.

1
Laboratrio de Sntese e Vetorizao de Molculas (LSVM), Univ.
Estadual da Paraba (UEPB)
2
Laboratrio de Sistemas Dispersos (LASID), Univ. Federal do Rio Grande
do Norte (UFRN)

Corresponding author: elquioeleamen@yahoo.com.br,

Introduction: Xylan is the second most abundant biopolymer in the plant
kingdom. It is the most common hemicellulose component in cell wall of
agricultural crops such as straw, sorghum, sugar cane and corn cobs.
Such polysaccharide can be hydrolyzed in colonic environment in human
body. This fact enables the use of xylan to produce colon specific drug
carriers. Objective: The aim of this work was to develop a new method to
produce xylan microparticles by emulsion cross-linking method using
sodium trimetaphosphate as cross-linking agent. Materials and Methods:
An alkaline solution containing xylan and sodium trimetaphosphate was
emulsified in 150 mL of liquid paraffin contain 7.5g of Span 80. The cross-
linking reaction took place at 50C with a constant stirring speed of
800rpm. After 6h of reaction, the microparticles were isolate and washed
with acetone, petroleum ether, deionized water and absolute alcohol by
means of centrifugation. Finally, the xylan microparticles were dried under
vacuum for 24h and kept in closed containers. Results and Discussion:
The results showed that xylan microparticles presented suitable
characteristics, with a good homogeneity. Regarding the visual aspect, the
sample appeared to be a yellowish powder. Microscopy analysis showed
that microparticles were spherical in shape and no agglomerates were
formed with a mean particle size of 7.7 0.17m. Conclusions: The
results showed that xylan microparticles produced with sodium
trimetaphosphate can represent an eligible colon specific drug delivery
system.

Financial Support from PROPESQ/UEPB and CAPES/Brazil


89

CODE 82
SYNTHESIS OF TIN (IV) COMPLEXES WITH POTENTIAL
ANTIMICROBIAL ACTIVITY

Guedes, J. S.; Barbosa, A. S. L.; Meneghetti, M. R.

Grupo de Catlise e Reatividade Qumica, Instituto de Qumica e
Biotecnologia, Universidade
Federal de Alagoas (UFAL)
Corresponding author: gessica_guedes@hotmail.com

Introduction. The discovery of cisplatin was a revolution in medicinal
chemistry. The formation of drugs containing metal elements in their
structures allows the development of a new series of synthetic drugs. One
potential candidate to such compounds, are organometallic tin complexes.
The association of tin compounds with bioactive ligands can be a suitable
strategy to the development of hybrid drugs, i.e. at least two pharmacofore
groups in the same drug.Objective. Synthesize of new tin organometallic
compounds bearing ligands with potential pharmacological activity.
Materials and Methods. The complexes n-Bu2SnCl2, Me3SnCl,
Me2SnCl2, n-Bu3SnCl, and t-Bu2SnCl2 were used as tin source
compounds, and the sodium salt of the nalidixic acid as bioactive ligand
precursor. The complexes were prepared from the reaction of the tin
source and the sodium salt of the nalidixic acid (Nal.Na) in toluene at
refluxed conditions for 24 hours under argon atmosphere. The mixture is
cooled down and chloroform is added, the solid is removed out and the
solution is concentrated under high vacuum. The desired products are
normally isolated as white solids. Yields around 80%. Results and
Discussion. Until now, all complexes were characterized by 1H NMR and
FTIR, giving strong evidences of the formation of the complexes n-
Bu2Sn(Nal)2, n-Bu3SnNal, Me3SnNal, and t-Bu2Sn(Nal)2. In the 1H
NMR can recognize signs of the ligand and the organotin used in the
synthesis. The antibacterial activity of these complexes are under
investigation. Conclusion. The methodology was simple and effective, the
potential antibacterial activity is under investigation.


90

CODE 83
ADVERSE DRUG REACTIONS AS A CAUSE OF HOSPITAL
ADMISSION

Medeiros, J. W. T.
1
; Oliveira, R. A.
1
; Pinto, C. O.
1
; Irineu, M. M.
1
; Barbosa,
M. M. S.
2
; Brito, A. E.
2

1
Academic of Pharmacy, Hospital Universitrio Onofre Lopes (HUOL),

2
Pharmacist, Hospital Universitrio Onofre Lopes (HUOL), Universidade
Corresponding author: joseffertrindade@yahoo.com.br

Introduction: The pharmacovigilance has the function to search and
monitor adverse drug reactions (ADRs), which according to World Health
Organization (WHO) is "a response to a drug that is noxious and
unintended and occurs at doses normally used in man for the prophylaxis,
dgnoss o hepy of dsese. One cn esme h 3-6% of all hospital
admissions are caused by ADRs and only 10% are reported. Objective:
This study aims to quantify the suspected ADRs that led to hospitalization
at Hospital Universitrio Onofre Lopes (HUOL). Methodology: The
suspected ADR as a cause of admission was evaluated through an active
search of medical records of patients newly admitted to the HUOL, a
teaching hospital, tertiary, with 189 beds in the period from January to
October 2011. Adverse reactions were classified according to causality and
severity. Results: 6 suspected adverse reactions were reported as cause
of hospitalization, of which one was classified as definite, 2 were probable
and 3 were possible. As for the gravity, one was lethal, one was severe
and 4 were moderate. Conclusion: One can observe that there is an
underreporting due to lack of diagnosis of ADRs at the time of
hospitalization and that the ADRs reported responsible by hospitalization
were severe, moderate and lethal. This is the first study in institution that
will provide the basis to encourage voluntary reporting by the physician at
the time of admission.

Financial support: unfunded.


91

CODE 84.1
ANTI-INFLAMMATORY ACTIVITY OF AQUEOUS LEAF EXTRACT OF
J atropha gossypiifolia L. IN CARRAGEENIN-INDUCED PAW EDEMA
IN RATS

Flix-Silva, J.1; Barbosa, L. M. Q.2; Gomes, J. A. S.1; Pinheiro, I. T. M.
G.1; Silva-Jnior, A. A.1,2; Zucolotto, S. M.2; Fernandes-Pedrosa, M.
F.1,2*

1
Laboratrio de Tecnologia & Biotecnologia Farmacutica (TecBioFar),
Departamento de Farmcia,
2
Programa de Ps-Graduao em Cincias Farmacuticas, Centro de
Cincias da Sade,
*Corresponding author: mpedrosa@ufrnet.br

Introduction: Jatropha gossypiifolia L. (Euphorbiaceae), popularly known
in Brazil as pnho-oxo, s vege spece used n fok medcne s n
anti-inflammatory, antiophidic, antipyretic, and healing drug. It is related in
the National List of Medicinal Plants of Interest to the Brazilian Public
Health System (RENISUS) due its recognized therapeutic potential.
Objective: Evaluate the systemic (oral route) anti-inflammatory activity of
aqueous leaf extract of Jatropha gossypiifolia (JG) and characterize its
preliminary phytochemical constitution. Materials and Methods: JG,
prepared by infusion, was first characterized by phytochemical screening
and thin layer chromatography. Then, it anti-inflammatory activity (50-200
mg/kg) was evaluated against carrageenin-induced paw edema in rats.
Extracts were administered orally one hour before carrageenin injection (1
mg/paw). Results and Discussion: Were detected alkaloids, phenols,
flavonoids, gums, resins, and sulfur compounds in JG, being flavonoids
(isoorientin and vitexin) identified as the possible major substances. At 100
and 200 mg/kg doses, JG were effective in reducing carrageenin-induced
paw edema (p<0.05), with intensity similar to standard anti-inflammatory
drug indomethacin. The peak effect occurred around the third hour after
injection of carrageenin, which corresponds to the period in which there is
principally production of prostaglandins in this model, suggesting an
inhibitory effect on cyclooxygenase action, in accordance with studies
showing that flavonoids have important inhibitory effect on arachidonic acid
metabolism. Conclusions: These results suggest, for the first time, the
potential of aqueous leaf extract of Jatropha gossypiifolia as a source of
molecules with anti-inflammatory activity, and seem to justify one of its
main popular uses.

Financial support: CNPq/Brazil.


92

CODE 84.2
ANTI-INFLAMMATORY ACTIVITY OF BULLFROG (Rana catesbeiana)
OIL IN CARRAGEENININDUCED PAW EDEMA IN RATS

Barbosa, L. M. Q.
1
; Flix-Silva, J.
2
; Gomes, J. A. S.
2
; Pinheiro, I. T. M. G.
2
;
Rocha, K. B. P.
3
; Fernandes-Pedrosa, M. F.1,
2
; Egito, E. S. T.
1
*

1
2
Departamento de Farmcia, Universidade Federal do Rio Grande do Norte
(UFRN)
3
Departamento de Patologia, Centro de Cincias da Sade, Universidade
*Corresponding author: socrates@pq.cnpq.br

Introduction: Bullfrog oil (BFO) is a mixture of lipid compounds extracted
from fat tissue from American bullfrog (Rana catesbeiana) legs. It is largely
used in Brazilian folk medicine as a topic or oral drug for treatment of
inflammation and others related diseases. Objective: Evaluate the
systemic (oral route) anti-inflammatory activity of BFO in rats. Materials
and Methods: The anti-inflammatory activity of BFO was evaluated
against carrageenin-induced paw edema in Wistar rats. One hour before
carrageenin injection (1 mg/paw), BFO was administered orally at 50, 100,
and 200 mg/kg, and progression of inflammation was measured by 4
hous. Hsophoogc nyss of s pws ws pefomed n ode o
investigate tissue changes after four hours of experiment. Results and
Discussion: BFO, at all doses tested, was effective in reducing
carrageenin-induced paw edema (p<0.05 compared to control group), in
the same magnitude of indomethacin (standard anti-inflammatory drug).
Histopathological analysis showed that there was no edema and only
minimal signals of inflammation in paws of BFO-treated rats. Higher doses
of BFO exhibited greater inhibitory effects than smaller doses, suggesting a
dose-dependent action. Conclusions: These results suggest, for the first
time, the potential of BFO as a source of molecules with antiinflammatory
activity, justifying one of its main popular uses.


\


93

CODE 86
INCLUSION COMPLEXES OF FUROSEMIDE WITH -CYCLODEXTRIN
AND PVP

Campos, M. L. G
(1)
, Silva-Jnior, A. A.
(1,2)*
.

1
Laboratrio de Tecnologia e Biotecnologia Farmacutica TecBioFar
Departamento de Farmcia UFRN.
2
Programa de Ps-Graduao em Cincias Farmacuticas da UFRN.
Corresponding author:bxmalulu@hotmail.com

Introduction: Furosemide is a diuretic drug used in the treatment of
hypertension. However, this drug is practically insoluble in water, and
strategies that may modify its physico-chemical properties as aqueous
solubility it is very important for production of the oral solid dosage forms.
Objective: The mainly aim of the present study was to investigate the
conditions for obtaining inclusion complexes of -FZ with the CD and
hydrophilic polymers such as PVP in solution. Materials and Methods:
The phase solubility study was performed to evaluate the complexation in
solution at 30 + 2C using the Higushi and Connors model. The
quantitative analysis of drug was performed by UV-VIS spectrophotometry
at 277nm (n=5). The analytical methodology was validated according to
analytical parameters established by ANVISA (Brazilian Sanitary Vigilance
Agency). The association of a third component, like PVP, was also
investigated. Results and Discussion: The solubility diagrams showed
the formation of inclusion complexes of FZ with B-CD with stoichiometry of
1:1 M with a stability constant of 166M-1. An increment in the FZ solubility
about 4 times was obtained. The association of B-CD (0.015 M) and the
PVP was increased solubility about 6 times compared to water.
Conclusions: The results indicate that the potential use of ternary
complexes and the effect of PVP on the increment of the solubility of the

Financial support: Brazilian Agency CNPq, PROPESQ-UFRN


94

CODE 87.1
RATIONAL USE OF DRUGS IN HYPERTENSION: AN ACHIEVEMENT
OF PHARMACEUTICAL CARE

Malheiro, J. P. T.
1
; Silva, P. C. D.
1
; Falco, R. A.
1
; Cartaxo, N. A. de O.
1*
;
Silva, S. A. S.
1
; Portela, A. S.
2
; Queiroz, M. S. R.
3
; Simes, M. O.
3

1
Discentes de Farmcia, Universidade Estadual da Paraba (UEPB)
2
Programa de Desenvolvimento e Inovao Tecnolgica de
Medicamentos, Universidade Federal do Rio Grande do Norte (UFRN)
3
Departamento de Farmcia, Universidade Estadual da Paraba (UEPB)
Corresponding author:* nathalia_cartaxo@hotmail.com

Introduction: The Pharmaceutical Care is a professional practice model
that uses the rational pharmacotherapy to achieve concrete results in
response to prescribed therapy. It requires of the pharmacist, the
translation of scientific knowledge and the promotion of rational use of
drugs, the main therapeutic tool. The irresponsible use of drugs may mask
symptoms or to propitiate drug interactions and adverse effects. Objective:
Based on this context, the study aimed to evaluate the use of medications
for hypertension assisted by a group of pharmaceutical care. Materials
and Methods: It was a cross-sectional study, whose sample consisted of
100 hypertensive patients assisted by the Program for Pharmaceutical
Care (PROATENFAR) of UEPB. The information in this analysis were
obtained by means of a questionnaire developed and validated previously,
applied by the same interviewer. The data were treated with the Statistical
Package for the Social Scienses (SPSS) version 17.0. Results and
Discussion: The drugs used by the sample covered 29 medicines at
doses as monotherapy or combined, the being drugs: hydrochlorothiazide
(44%), captopril (33%), amlodipine (20%), Aspirin (26%), Atenolol (14%)
and Propatilnitrato (11%), the most frequent. In the absence of any of the
medicines available by the public service, over 90% of the sample said
they did not discontinue therapy, using loans or purchases. And, 39.65%
said the pharmacist or pharmacy students of PROATENFAR as
information providers on the rational use of medicines. Conclusions:
Thus, these results reinforce the importance and effectiveness of
pharmaceutical care in patient adherence and in the promotion of rational
use of medicines.

Financial support from: Programa de Incentivo Ps-Graduao e
Pesquisa (PROPESQ), UEPB


95

CODE 87.2
EXTRACTION OF FLAVONOIDS AND POLYPHENOLS IN LEAVES OF
Mentha piperita L. PROCESSED IN FIXED BED CONVECTIVE DRYER

Cartaxo, N. A. de O.
1
Oliveira, A. R.
1
Apolinrio, A. C.
1
Fialho, G. J. D.
1
Feitosa,V.A.
1
Pach, C. O.
1,

1
Departamento de Farmcia, Universidade Estadual da Paraba (UEPB);

INTRODUCTION: Mentha x piperita L. is a species cultivated in semi-arid
and traditionally used popular medicine. Snce s used by he SUS, should
to observe the optimization of the drying process for preservation of
bioactive compounds. OBJECTIVE: Study the drying process leaves
Mentha x piperita L. and its effects on the extraction of polyphenols and
flavonoids. METHODS: The study was conducted at the Laboratrio de
Meios Porosos e Sistemas Particulados at UFCG, Campina Grande PB,
from September/2009 to May/2010. Once collected, the samples were
identified by the Herbrio Lauro Pires Xavier (N 41899). The leaves were
dried in a fixed bed convection dryer at temperature of 40, 50 e 60C and
then macerated using 70% ethanol solution. In the resulting extracts were
carried out determinations of the compounds, for spectrophotometry, total
polyphenols and total flavonoids, using the Folin-Denis standard tannic
acid (760nm) and aluminum chloride solution - standard quercetin
(425nm), respectively. RESULTS: The drying proved to be fast, being
completed at intervals of 125, 110 and 100 minutes on average to
temperatures of 40, 50 and 60C, respectively. Quantification of total
polyphenols in average was 18.71, 13.36 and 9.43 g/mL for 40, 50 and
60C, respectively. For total flavonoids quantified the mean was 9.80, 7.01
and 5.44 g/mL for 40, 50 and 60C, respectively. CONCLUSIONS: The
leaves of Mentha x piperita L. had higher concentrations of the compounds
quantified in the temperature of 40C in fixed bed convection dryer, proving
the best methodology.

Work funded by the Programa de Iniciao Cientfica / UEPB.


96

CODE 90
DISTRIBUTION OF TRIATOMINES FAUNA AND NATURAL INFECTION
BY Trypanosoma cruzi IN THE SEMIARID OF THE STATE OF RIO
GRANDE DO NORTE, BRAZIL

Silva, A. N. B.
1
; Martins, K.
2
; Cmara, A. C. J.
1, 2
; Chiari, E.
3
; Galvo, L. M.
C.
1, 4
;

1.
Programa de Ps-Graduao em Cincias Farmacuticas, CCS, UFRN
2.
Departamento de Anlises Clnicas e Toxicolgicas, CCS, UFRN
3.
Departamento de Parasitologia, ICB, UFMG
4
. Programa de Ps-Graduao em Cincias da Sade, CCS, UFRN
Corresponding author:andressanb@hotmail.com

Introduction: The predominant triatomines species with potential for
transmitting Trypanosoma cruzi and ability for adapting to various ecotopes
in the semiarid are Triatoma brasiliensis and T. pseudomaculata.
Objective: To evaluate the triatomines fauna and natural infection by T.
cruzi in different semiarid environments. Materials and methods: The
insects were captured manually in peridomicile, intradomicile and wild
environment of different municipalities in west and central mesoregions.
The insects were identified and examined by the direct method and
xenoculture for detecting T. cruzi. Results and discussion: Of the 779
captured triatomines, 43.4% were T. brasiliensis (59.8% adults and 40.2%
nymphs), followed by 27.6% of T. pseudomaculata (83.7% adults and
16.3% nymphs), 26.2% of Panstrongylus lutzi (only adults) and 2.8% of
Rhodnius nasutus (63.6% adults and 36.4% nymphs). The species
captured in intradomicile were T. brasiliensis (95.5%) and T.
pseudomaculata (4.5%). In peridomicile were found T. brasiliensis (52.3%),
T. pseudomaculata (35.4%) and R. nasutus (12.3%). In wild environment
were captured 36.4% of T. brasiliensis, 36.6% of P. lutzi and 27% of T.
pseudomaculata. The rate of natural infection of triatomines by T. cruzi was
9.8%. The highest infection rate, 29.9%, was detected in P. lutzi, while T.
brasiliensis was 4.1%, T. pseudomaculata 3.2% and R. nasutus 0.0%.
Conclusion: T. brasiliensis and T. pseudomaculata were captured in
different stages and in all environments. Infected nymphs in the
peridomicile and adults inside the houses show the adaptability to
anthropic environment and their potential as vector, which suggests the
need for continuous epidemiological surveillance actions.

Financial support from CNPq/MCT/CNPq; FAPERN/PPSUS;
DCR/CNPq/FAPERN


97

CODE 91
DEVELOPMENT OF THIOPHENES DERIVATIVES BIOCOMPATIBLES
MICROEMULSIONS

Pedrosa, M.O.
1
; Arajo, G.M. F.
1
; Silva, N.E.S
2
.; Patriota, Y.B.G.
2
; Pereira,
R.C.
2
, Silveira, W.L.L.
3
. Ferreira, L.F.
3
, Silva-Filho, M.A.
3
, Egito, E.S.T.
3
,
Mendona-Jr, F.J.B.
4
, Silva, J.A.
1
, Damasceno, B. P. G. L.
1

1
Universidade Estadual da Paraba (UEPB), Programa de Ps-Graduao
em Cincias Farmacuticas (PPGCF), Laboratrio de Desenvolvimento e
Caracterizao de Produtos Farmacuticos (LDCPF), 58429-600,
Campina Grande-PB-Brazil,
2
UEPB, LDCPF, Departamento de Farmcia, 58429-600, Campina
Grande-PB-Brazil.
3
Universidade Federal do Rio Grande do Norte (UFRN), Departamento de
Farmcia, Laboratrio de Sistemas Dispersos (LASID), 59010-180, Natal-
RN-Brazil.
4
UEPB, PPGCF, Laboratrio de Sntese e Vetorizao de Molculas
(LSVM), Joo Pessoa-PB-Brazil.

Introduction: Thiophenes derivatives (TD) are strong insoluble molecules
and have important structural fragment of many pharmaceutical
compounds (aromatic heterocyclic group). They exhibit significant action
against fungals, bacterias, viruses, worms and insects. Several TD were
synheszed by UEPBs esech goup, keLSVM-5CN06 and LSVM-
6CN10. Objective: To develop and to characterize a LSVM-5CN06oil-in-
water microemulsion(LSVM-5CN06-ME) and LSVM-6CN10 oil-in-water
microemulsion (LSVM-6CN10-ME). Materials and Methods: The ME was
prepared by a direct mixture of four inactive ingredients: Lipoid
S 100,
Tween
80, Mygliol
812 N and distilled water.The TD were previously

dissolved in chloroform and added under stiring into the ME. The solvent
was evaporated by a rotative evaporator, and the macroscopic aspect, pH
and conductivity were analyzed. Results and Discussion: The solubility
test has shown that LSVM-5CN06 was insoluble in acetronitrile and
methanol, and soluble in chloroform; the LSVM-6CN10 was sparingly
insoluble in acetronitrile and methanol, and soluble in chloroform. The pH
values were 5,680,12 and 7,590,016 to LSVM-5CN06-ME and LSVM-
6CN10-ME, respectively.To the electrical conductivity, the values
were97,8323,39 and 625,97,41 to LSVM-5CN06-ME and LSVM-6CN10-
ME. Conclusions: These results allow us to conclude that ME is a good
delivery system to LSVM-5CN06 and LSVM-6CN10. However, other future
aspects need to be investigated for a better analysis of the system.

Financial support from PROPESQ/UEPB.


98

CODE 92
INFLUENCE OF PLURONIC
F68 ON THE BENZATHINE PENICILLIN G

ANTIBACTERIAL ACTIVITY

Silva, K. S.; Silva, K. G. H; Silva, K. C. H; Alexandrino Junior, F;
Godoy, J. S. R; Svidzinski, T. I. E; Egito, E. S. T
#
.

1
, Laboratrio de Sistemas Dispersos, Departamento de Farmcia,

2
Laboratrio de Micologia Clnica, Departamento de Farmcia,
Universidade Estadual de Maring (UEM)
Corresponding author:socrates@ufrnet.br
#

Introduction: Polymer-based nanotechnology became one of the most
attractive and fast growing areas of pharmaceutical research. In aqueous
solutions at concentrations above critical their micelle concentration (CMC)
these polymers self-assemble into micelles. Objective: The purpose of
this study was to evaluate the antibacterial activity of Benzathine Penicillin
G (BenGP) into a micellar solution (MS) of Pluronic
F68. Materials and

Methods: Firstly the MS of Pluronic
F68 was prepared in water at its CMC

of 1.23x10
-4
M at 37C. Antimicrobial activity of BenGP (500g/mL) into
these Micellar Solution (MSBenGP) was evaluated by the determination of
minimum inhibitory concentration (MIC) using such as blank the
Pluronic
F68 MS and as a standard a BenPG solution at the same

concentration against S. pyogenes ATCC19615, S. mutans ATCC25175,
S. faecalis ATCC51299(1), S. faecalis ATCC29212(2) and S. aureus
ATCC25923, cultivated as described in the Clinical and Laboratory
Standards Institute. Results and Discussion: The measurement of the
MICs for S. pyogenes was reduced from 1.953 to 0.488g/mL, S. mutans
from 1.953 to 0.122g/mL, S. faecalis(1) from 0.977 to 0.244g/mL, S.
faecalis(2) from 0.977 to 0.061g/mL and S. aureus from 7.813 to 0.976
g/mL using MSBenPG. The MS without of BenPG did not show
antimicrobial activity. These results showed that MS enhances antibacterial
activity of BenGP for all tested strains. Conclusions: There are, MS could
be useful for treatment of infectious diseases against these
microorganisms, leading to a significant reduction of drug used during a
treatment, which also may decrease the number of injections and improves
the patient compliance to the treatment.

Financial Support from CNPq/Brazil and master scholarship from
CAPES/Brazil (Procad NF 2008)


99

CODE 93
EVALUATION OF THE ANTIFUNGAL ACTIVITY OF NOR-LAPACHONE
DERIVATIVE AGAINST ISOLATES OF CANDIDA spp.

Th, M. F. D. F.
1
; Santos Neta, M. A.
1,2
; Silva, C. R.
1,2,
; Andrade Neto, J
.B.
1
; Macedo, D. S.
2,3
; Silva Jnior, E. N.
4
; Pinto, M. C. F. R.
4
; Macdo, D.
S.
3
; Cavalcanti, B. C.
3
; Magalhes, H. I. F.
3
; Moraes, M. O.
3
; Nobre Jnior,
H. V.
1,2,3

1
Department of Clinical and Toxicological Analysis, School of Pharmacy,
Laboratory of Bioprospection and Experiments in Yeast (LABEL). Federal
University of Cear, Fortaleza, CE, Brazil.
2
Department of Pathology and Legal Medicine, School of Medicine,
Specialized Medical Mycology Center, Federal University of Cear,
Fortaleza, CE, Brazil.
3
Department of Physiology and Pharmacology, Federal University of
Cear, Fortaleza, CE, Brazil.
4
Department of Chemistry , Federal University of Minas Gerais, Belo
Horizonte, MG, Brazil
Corresponding author: helioufc@yahoo.com.br

Introduction: Nor-lapachones, the inferior homologue of the -
lapachones, have been showed important biological properties. In a
program for obtaining naphthoquinones more potent against Candida spp.,
we prepared a new compound (NPPN 3223) from 2-Allyl-3-hydroxy-
[1,4]naphthoquinone. In recent investigation, NPPN 3223 was active
against Trypanosoma cruzi (he eoogc gen of he Chgs dsese)
and Lymphoma T Murine EL-4 cells. Objectives: Herein, the antifungal
potential of NPPN 3223 against 04 strains of Candida spp was evaluated.
Methods: A total of 04 strains of Candida spp. belonging to the Laboratory
of Bioprospecting and Experimentation in yeast (LABEL) from Federal
University of Cear were used. The antifungal susceptibility testing was
performed by broth microdilution method, according to the
protocol of CLSI M27-A3. NPPN 3223 was tested at concentration ranging
from 0.25 to 128 mg / mL. Strains of C.parapsilosis (ATCC 22019) and
C.krusei (ATCC 6258) were used as control strains.
Results and Discussion: The NPPN 3223 showed antifungal activity
against all strains of Candida spp. Conclusion: The obtained results
showed, under our experimental conditions, a cytotoxity action of
NPPN 3223 on all Candida strains evaluated. However, studies with large
numbers of strains are needed.

Financial Support from FUNCAP, CNPq.


100

CODE 94
QUALITATIVE PHYTOCHEMICAL PROFILE OF J atropha gossypiifolia
L. EXTRACTS PREPARED BY DIFFERENT METHODS OF
EXTRACTION

Gomes, J. A. S.
1
; Flix-Silva, J.
1
1
; Zucolotto, S. M.
2
;
Fernandes-Pedrosa, M. F.
1*

1
Departamento de Farmcia, Universidade Federal do Rio Grande do Norte
(UFRN)
2
Laboratrio de Farmacognosia, Departamento de Farmcia, Universidade
Corresponding author: mpedrosa@ufrnet.br

Introduction: Jatropha gossypiifolia L. (Euphorbiaceae), popularly known
n Bz s pnho-oxo, s vege spece used n fok medcne s n-
inflammatory, antimicrobial, antiophidian, antipyretic, and hypotensive
drug. Substances such as flavonoids, terpenoids and lignans have been
isolated from different parts of the plant, however, the literature lacks
systematic studies that differentiate the chemical composition of each plant
organ, and the majority of these reports does not discern or specify which
method of extraction was employed. Objective: Determine and compare
the phytochemical profile of leaf, stems, and roots extracts from J.
gossypiifolia prepared by different methods of extraction aiming future
studies in screening of bioactive compounds. Materials and Methods:
Leaves, stems and roots were dried at 60C and pulverized. Extracts of
each part, separately, were prepared at 10% w/v (dry plant: solvent
extractor) by infusion, decoction, ultrasound bath (using water as solvent
extractor) and maceration (ethanol). These extracts obtained (twelve
extracts) were compared qualitatively by phytochemical screening and thin
layer chromatography. Results and Discussion: Alkaloids, flavonoids,
gums, lactones, quinones, resins, sulfur compounds, tannins, and
terpenoids were identified in J. gossypiifolia extracts. In general, leaf
extracts presented better results (greater number of substances identified)
than stems and roots, and aqueous extracts (even if obtained at different
methods) showed similar profiles. Flavonoids were identified as the
possible major substances of leaf extracts. Conclusions: This study
highlights the potential of the specie as a source of bioactive molecules,
opening ways for the study of this plant in order to obtain new therapeutic
alternatives.



101

CODE 95.1
EVALUATION OF ANTIFUNGAL ACTIVITY OF CRUDE EXTRACT
FROM Caesalpinia ferrea Mart AGAINST CLINICAL ISOLATES FROM
THE ORAL CAVITY OF RENAL TRANSPLANTATION

Ferreira, M. R. A.
1
; Santiago, R. R.
1
; Diniz, M. G.
1
; Milan, E. P.
1
; Faria, M.
G. I.
2
; Mello, J. C. P.
3
, Svidzinski, T. I. E.
2
; Soares, L. A. L.
1,4*

1
Programa de Ps graduao em Cincias Farmacuticas,

Universidade
2
Laboratrio de Micologia Mdica,

Departamento de Anlises Clnicas e
Biomedicina,

3
Departamento de Farmcia e Farmacologia,

Universidade Estadual de
Maring (UEM).
4
Departamento de Cincias Farmacuticas,

Universidade Federal de
Pernambuco(UFPE).
Corresponding author:lals@gmx.de

Introduction. The increases in the drug resistance as well as the several
side effects from current antifungal therapy reinforce the importance and
the potential of plants extracts and their secondary metabolites as
alternatives for new, more effective and less toxic treatments. Objective. In
this context, the aim of this study was to evaluate crude extracts of C.
ferrea against clinical isolates from the oral cavity of renal transplantation.
Materials and Methods. The extractive solutions were obtained by turbo
extraction (water or acetone:water, 7:3) and then were filtered,
concentrated and lyophilized to yield the crudes extracts (CE). The CE
were evaluated against 91 isolates (Candida spp.) obtained from Natal/RN
and Maring/PR. The minimum inhibitory concentration (MIC) was
determined according to CLSI with adaptations for natural products. The
data were analyzed by the MIC, MIC
50
and MIC
90
. Results and
Discussion. The both CE (aqueous and acetone:water) of C. ferrea
showed the same MIC (ranged from 3.9 to 1000g/ml), MIC
50
and MIC
90

(125 and 500g/ml, respectively), against C. albicans. Concerning the
activity against C. non-albicans the CE extracts showed similar
performance, and the MIC was ranged from 3.9 to 500g/ml, while MIC
50

and MIC
90
were 31.25 and 500g/ml, respectively. Conclusion. To
conclude, the significant antifungal activity observed for both CE extracts,
suggest that the plant species can play an important role as a potential
antifungal agent. However, complementary studies are necessary to obtain
fractions and isolated compounds.

Financial support: CAPES and CNPq.


102

CODE 95.2
BIOASSAY-GUIDED PURIFICATION OF CRUDE EXTRACT FROM
STEM BARKS FROM Caesalpinia ferrea Mart (PAU FERRO)

Ferreira, M. R. A.
1
; Mello, J. C. P.
2
; Svidzinski, T. I. E.
3
; Soares, L. A. L.
1,4*

1
Programa de Ps graduao em Cincias Farmacuticas,

Universidade
2
Departamento de Farmcia e Farmacologia,

Universidade Estadual de
Maring (UEM).
3
Laboratrio de Micologia Mdica,

Departamento de Anlises Clnicas e
Biomedicina,

4
Departamento de Cincias Farmacuticas,

Universidade Federal de
Pernambuco(UFPE).
Corresponding author:lals@gmx.de

Introduction. Caesalpinia ferrea (Caesalpiniaceae) is a tree native from
Brazil, known as pau ferro. Because of its widespread use, research is
needed to identify which are the active compounds of the species.
Objective. The aim of this study was to investigate an antifungal activity by
the bioassay-guided of a crude extract (CE) from C. ferrea. Materials and
Methods. The steam barks of C. ferrea were submitted to turbo extraction
with acetone:water (7:3), yielding the CE. The CE was partitioned with
ethyl-acetate/water (1:1), resulting in aqueous (FW) and ethyl-acetate
(FEtOAc) fractions. The FEtOAc was subjected to column chromatography
(Sephadex
LH-20) with an eluent gradient EtOH (50%, 100%), MeOH

(50%, 100%) and acetone:water (7:3), to yield 12 subfractions (F1-F12).
The fractionation was monitored by thin layer chromatography (TLC) and
the antifungal activity was evaluated by the CLSI using strains ATCC of
Candida spp. Results and Discussion. The CE was partitioned yielding
the FA and FEtOAc. The evaluation of the antifungal activity showed that
the FEtOAc was more active, with MIC between 0.9731.25g/ml. Thus,
the FEtOAc was selected for new purification process and 12 subfractions
were obtained. The subfractions were monitored by TLC and antifungal
activity, and the fraction from F8-F11 presented most intense spots on
TLC, typical for polyphenolic compounds of high molecular weight, and
even better antifungal activity, which MICs were ranged from 1.95-
250g/ml. Conclusion. The bioassay-guided purification of crude extract
from C. ferrea suggested that the antifungal activity seem to be attributed
to the polyphenolic composition of the drug material.

Financial support: CAPES and CNPq.


103

CODE 96
IDENTIFICATION AND PHOSPHOLIPASE ACTIVITY OF FUNGI
ISOLATED FROM DERMATOMYCOSES OF CHILDREN ATTENDING
MUNICIPAL CENTERS OF CHILDREN EDUCATION FROM NATAL-RN

Silva, W.P
1
; Lemos, V.L.B
1
; Chaves, G.M
1

1
Laboratrio de Micologia Mdica e Molecular Departamento de Anlises
Clnicas e Toxicolgicas Universidade Federal do Rio Grande do Norte
(UFRN)
Corresponding author:ranisonbass@hotmail.com

Introduction: Dermatomycoses are superficial mycoses which damage
the cutaneous tissue. They are extremely prevalent among agglomerated
environment, where people share personal objects. Objective: The aims of
the present study were to isolate and investigate phospholipase among
fungi obtained from superficial infections from children who attend
Municipal Centers of Children Education, at Natal-RN. Materials and
Methods: Children from 0 to 6 years old were evaluated. They belonged to
the foundations: Amor de Me, at Praia do Meio; Maria Eullia, at Passo
da Ptria community and Professora Maria Itacira Bento, from Cidade da
Esperana neighborhood. Clinical samples were collected from children
showing lesions probably due to dermatomycoses. Samples were smeared
on the surface of Sabouraud Dextrose Agar with Cloramphenicol (100
mg/L), and incubated at room temperature (26 + 1 C), for up to 15 days.
Yeasts identification was performed with the chromogenic medium
CHROMagar Candida, microculture slides and classic methods.
Phospholipase activity was determined by growing yeast cells on the
surface of phospholipase agar. Results and Discussion: We collected
samples from 102 patients, and obtained 55 positive results (54%).
Positive results are distributed as follows: 22 yeasts from the genus
Trichosporon (40%), 10 yeasts from the genus Malassezia (18.2%), 10 C.
parapsilosis (18.2%) 7 C. albicans (12.7%), 3 P. ohmeri (5.45%), 1 C.
tropicalis (1.82%), 1 Cryptococcus sp. (1.82%) and 1 isolate of T. rubrum
(1.82%). Conclusions: C. albicans isolates had high phospholipase
activity. C. parapsilosis and P. ohmeri strains showed intermediate levels
of phospholpase activity. C. tropicalis, Cryptococcus sp. and Trichosporon
spp. Isolates showed low or none phospholipase activity.
.


104

CODE 97
EVALUATION OF THE REPRODUCTIVE AND FETAL TOXICITY OF
CYLINDROSPERMOPSIN IN THE RAT

Almeida, C.S.
1
; Arruda, A.C.C.
1
; Queiroz, E.C.
1
; Costa, H.T.M.L.
1
; Barbosa,
P.F.
1
; Lemos, T.M.A.M.
2
; Oliveira, C.N.
3
; Schwarz, A.
1
; Pinto Ernani
4
;
Kujbida, P.
1

1
Laboratrio de Toxicologia, Departamento de Anlises Clnicas e
2
Laboratrio de Bioqumica Clnica, Departamento de Anlises Clnicas e
3
Departamento de Patologia, Universidade Federal do Rio Grande do
Norte (UFRN).
4
Laboratrio de Toxinas e Produtos Naturais de Algas, Departamento de
Anlises Clnicas e Toxicolgicas, Universidade de So Paulo (USP).
Corresponding author: paulask@ufrn.br

Introduction: Cylindrospermopsin (CYN) is a naturally occurring alkaloid
produced by different genera of freshwater cyanobacteria, e.g.
Cylindrospermopsis, Umezakia, Aphanizomenon, Raphidiopsis. The CYN
toxicity is characterized by fatty degeneration of the liver, cholestasis, injury
to renal tubules and thymic atrophy. Objective: In the present study, the
potential of CYN to induce toxic effects and to impair gestation in rats
exposed orally during pregnancy was investigated. Materials and
Methods: The animals were exposed to 0.03, 0.30, 3.00 g CYN/kg of
body weight/day, for 21 days, by oral administration (gavage). These
doses were lower than the no-observed-adverse effect level (NOAEL) used
to establish the guideline for a safe level of drinking water for this toxin (1
g/L) by World Health Organization (WHO). Females were sacrificed on
day 21 of gestation, when maternal body and organ weights were
recorded, serum and biochemical analyses were performed, reproductive
parameters (number of pregnant rats, body and reproductive organ weight,
number of implantation sites, viable fetuses, and resorption sites) were
determined and skeleton and viscera of fetuses were evaluated. Results
and Discussion: The reproductive performance and teratology results
were similar between control and exposed group, however, cyanotoxin
administration increased the size of the kidneys and the urea rates in
pregnant rats. Conclusions: Our findings suggest that the consumption of
CYN in the concentration recommended by WHO is not able to promote
fetotoxicity or affect the reproductive performance of rats. On the other
hand, orally administered CYN may induce initial nephrotoxicity in pregnant
mammals.

CAPES/Brazil


105

CODE 98.1
DEVELOPMENT OF A LIQUID DETERGENT FORMULATION FOR USE
IN UFCG/CES AND EVALUATION OF VISCOSITY AND POWER FOAM

Azevedo, M. G. B; Almeida, M. A. A. L. S.; Batista, M. T.; Souza, J. B. P.;
Alencar, J. S.

Farmcia Escola Manoel Casado de Almeida, Curso de Bacharelado em
Farmcia, Universidade Federal de Campina Grande/Centro de Educao
e Sde, Oho Dgu d Bc, s/n, Cu, PB, 58175-000.
Corresponding author: gloria.farmacia@hotmail.com

Introduction: The cleaning materials are sanitizing consumed in large
scale, including in universities, in which the bidding processes for
acquisition, takes time-consuming and often are products of low quality and
yield. Among these articles, the liquid detergent is one of the most
consumed products by UFCG/CES.Objective: Aiming this need, the
objective of this study is to develop a formulation of liquid detergent to be
used in UFCG/CES, evaluating viscosity and foaming power. Materials
and Methods:Thus, twelve formulations were prepared asa pilot
formulation.The first eight contain surfactants, stabilizers, hidrtopos,
preservatives and additives, with the concentration of the agent variable
viscosity. The remaining four used identical components to those used in
the previous group, varying only the concentration of primary surfactant
Sodium laureth-2 sulfate (LESS).Results and Discussion: The
manipulation of the first formulations allowed the construction of the Critical
Micelle Curve (CMC) and showed that regardless of the concentration of
the thickener was impossible to obtain the ideal viscosity for samples of
commercial reference. In the subsequent four, set the viscosity with
increasing concentration of less, thus obtaining the optimal formulation
consisting of sulfonic acid (5%), Sodium hidroxide (1%), Triethanolamine
(0.4%), LESS (5%), amphotericbetaine (1%), Dietanolamida (0.5%),
Viscopon (1.5%), urea (1%), isothiazolinone (0.25%), core (qs), essence
(0,1%) and NaCl (0.8%), with medium viscosity of 413 cP and foam height
of 5.5 cm, compatible with the benchmark. Conclusions:The success of
this formulation meets the need of that educational institution, providing a
product with high-quality in relation to the bid, seeking a better cost-
effective.


106

CODE 98.2
RETROSPECTIVE STUDY OF ADVERSE DRUG REACTIONS IN THE
PEDIATRIC WARD OF THE UNIVERSITY HOSPITAL ALCIDES
CARNEIRO, CAMPINA GRANDE / PB

Almeida, M. A. A. L. S.
1
; Azevedo, M. G. B.
1
;

Oliveira, H. F. A.
1
; Jcome, R.
C.
1
; Neto, A. R. M.
2
; Lima, D. D. F. S.
1
; Farias, A. D.
1
; Porto, M. L. S.
3

1
Universidade Federal de Campina Grande/Centro de Educao e Sade,
Oho Dgu d Bc, s/n, Cu, Pb, Bs Em:
2
Acad. Enfermagem, Universidade Estadual da Paraba, Campina Grande,
Paraba
3
MSc Farmacutica-Bioqumica - gerente de risco do Hospital Universitrio
Alcides Carneiro, Campina Grande, Paraba
Corresponding author: cida_alm@hotmail.com

Introduction: The limitation in pre-clinical and clinical studies on the use of
medicines in children, often leads the profissional to interpret the
prescribing drug therapy in a pediatric equivalent of what is practiced in
adults. This fact compromises the result and safety of treatments, and can
trigger Adverse Drug Reactions (ADRs). Objective: To describe the main
ADRs n pedc Unvesy Hosp Acdes Cneo membe of he
Sentinel Network of National Health Surveillance Agency (ANVISA).
Material and Methods: Retrospective and descriptive study, based on
reporting forms developed by ANVISA and recorded through an active
search on Sentinel Hospital during the period 2002 to 2010. Study was
carried out with the aid of didactic literature and the electronics teaching
one. Results and discussion: During that time, 52 notifications were
made, these were 31.0% in pediatrics. In that sector it was noted that the
ADRs hives were more frequent (46.6%), pruritus (20.0%), fever (13.3%),
vomiting, edema, headache, tremors pyrogenic shock, and covered 6.6%
of ADRs. The most common drugs responsible for that damage was the
sodium dipyrone and ceftriaxone in 53.3% of total pediatrics ADRs.
Children can vary pharmacokinetic and/or pharmacodynamic, that may
lead to an increase or decrease in sensitivity to some drugs, whose causes
are not always known. Conclusion: The study shows that such changes
require the use of medications in children should be further studied and
monitored by the pharmacist, during therapy.


107

CODE 99
IN VITRO RELEASE OF METHOTREXATE-LOADEDSPRAY DRIED
PLGA MICROPARTICLES

Oliveira, A. R.
1,2
, Abrantes, J. A.
1,2
, Oliveira, A. G.
3
, Silva-Junior, A. A.
1,2
.

1
Pharmaceutical Technology and Biotechnology Laboratory, Federal
University of Rio Grande Norte (UFRN), Natal Brazil;
2
Graduate Program in Pharmaceutical Sciences (UFRN), Natal Brazil;
3
Graduate Program in Pharmaceutical Sciences (UNESP) Araraquara
SP Brazil.
Corresponding author:farmalix@yahoo.com.br

Introduction: Methotrexate is a chemotherapeutic that has a rapid
plasmatic elimination. Biodegradable microparticles have been extensively
investigated to prolong the drug release. The study of drug release profile
is essential to ensure success and safety of these drug delivery systems.
Objective: To assess the in vitro release profiles of different PLGA
microparticles containing MTX obtained by spray drying.Materials and
Methods: MTX-loaded PLGA microparticles were obtained in a mini spray
dryer Buchi-191 at different ratios drug/polymer (10, 20, 30 and 50% w/w).
The in vitro release studies were performed in phosphate buffer pH 7,4
(0.05M KH
2
PO4) medium thermostatized 37C 0,2C. At established
intervals, the tubes were centrifuged and then the supernatant were
analyzed by UV-Vis spectrophotometry. Cumulative drug released were
fitted using different kinetic release models: zero order, fist order, Higuchi
and Weibull treatments. Results and Discussion: The in vitro release
studies performed for all microparticles systems shown a MTX sustained
release. The time necessary to release all drug content was 1, 2, 8 hours
for PLGA microparticles containing 50, 30 and 20% w/w MTX respectively.
However, the time necessary for release about of 80% of drug content in
the microparticles containing 10%w/w/ of drug was 1008 hours. The
analysis of drug release revealed a prevalent Weibull model, which was
evident by the correlation coefficient (r
2
= 0.985).Conclusions: PLGA
microparticles containing methotrexate were successfully obtained by
spray drying technique, which demonstrating an extended release profile
for all drug-polymer ratios used.

Financial support: Brazilian Agency CNPq, CAPES


108

CODE 101
BENZNIDAZOLE-LOADED SOYBEAN O/W EMULSION SYSTEM

Streck, L.
1*
; Arajo, M. M.
1
; Santos, S. R. A.
2
; Souza, I
2
; Oliveira, A G
3
;
Silva-Jnior, A.A.
1

1
Federal do Rio Grande do Norte, UFRN, Natal, RN
2
Curso de Farmcia, UFRN, Natal, RN
3
Faculdade de Cincias Farmacuticas de Araraquara, UNESP,
Araraquara, SP
Corresponding author:leticiastreck@gmail.com

Introduction: Benznidazole (BNZ) is not only the drug of choice for
Chags dsese emen, bu nowdys s so he ony one dug
avaible in Brazil. However, BNZ is poorly soluble drug and it is available
just as tablets. Objective: Developing a soybean o/w emulsion system
(ES) containing BNZ. Materials and Methods: Soybean oil was use as oil
phase and a mixture of span 80 (Sigma Aldrich, EUA) and tween 80
(Synth, Brazil) as surfactants, deionized water as aqueous phase and BNZ
(Roche, Brazil). An excess of BNZ was loaded using the ready emulsions
at room temperature or added on inversion phase moment at 70C. The
effect of different co-surfactants such as ethyl alcohol, benzyl alcohol and
methyl-1 pyrrolidone-2 and the proportion of the components were
investigated, which was estimated using UV-Vis epectrophotometry,
validated previously, and varying proportions of the components from ES
were studied when modified the ratios of soybean oil and surfactant
mixture. Results and Discussion: BNZ loaded in the O/W ES was about
0.04 and 0.039% for technique at room temperature and and 70C,
respectively. The different methods and co-surfactants used did not
provoke statistical differences on drug loaded. Increasing the rate of
soybean oil declined the BNZ loaded however, the rate surfactants studied
did not induce changes in the BNZ loaded. Conclusions: The
experimental results showed that the different methods and surfactants
used did not change statistically the drug loading. The oil phase increment
leads to decreasing of these levels.

Financial support: CNPQ and CAPES


109

CODE 102
EVALUATION OF CITRONELLA (Cymbopogon winterianus JOWITT
EX BOR.) OIL COMPOSITION EXTRACTED FRESH AND DRY PLANT
AND INFLUENCE OF HARVESTING PERIOD.

Silva, M. E.
1
; Carbogim, L. G. S.
1
; Silva, T. M.
2
; Amaral, M. P. H.
1
; Cheider,
L. M.
3

1Pharmacotechniques Laboratory, Pharmaceutical Department, Juiz de
Fora Federal University (UFJF);
2Food and Toxicology Laboratory, Food and Toxicology Department , Juiz
de Fora Federal University (UFJF).
3Phytochemistry Laboratory, Botany Department, ICB, Juiz de Fora
Federal University (UFJF).
Corresponding author:milenee@gmail.com

Introduction: The essential oil extracted from leaves of citronella grass
(Cymbopogon winterianus Jowitt ex Bor.) has aromatic and therapeutic
features, and raw material for the industry in repellents and toilet
preparations. Among the many factors that can influence the chemical
composition of volatile constituents stand out from the time of harvest and
drying process. Objective: Evaluation of the influence of harvest time and
drying the composition of oil, compared with the fresh plant. Materials and
Methods: Plant Origin: Medicinal Plants Garden, Pharmacy Faculty, UFJF.
Harvesting periods: August 2010, January and April 2011. For this study,
extractions were performed by hydrodistillation in Clevenger apparatus, of
fresh leaves (FL) and dried leaves (DL) between 32-35C for 7 days. The
essential oil samples were analyzed by GC/MS. Results and Discussion:
During August 2010, it was observed in the essential oil of FL the presence
of -muroleno, -cadinene and farnesol and in April 2011, in the essential
oil of DL, the presence of cis-2,6-dimethyl-2,6-octadieno-acetate and
geno, h wsn found n ohe peods. The gee qunes of found
components in all samples were citronellal, geraniol and citronellol.
Regarding the drying process, there was a reduction in the number of
substances identified from the DL, when compared with FL. Conclusion:
The different times of harvest influenced the number of components found
in essential oils. The drying method affected the essential oils causing a
reduction in the number of components detected in samples taken from the
dried leaves.

Financial support from: Pharmaceutical Department,
Pharmacotechniques Laboratory - UFJF


110

CODE 103
SCREENING FOR ANTIBACTERIAL ACTIVITY ANTIMICROBIAL
ACTIVITY OF HYDROALCOHOLIC EXTRACT OF Anadenanthera
macrocarpa Benth AND Myracodruom urundeuva All. AGAINST
STRAIN OF Staphylococcus aureus

Almeida, C. L. F.
1
; Nunes, L. E.
1
; Fernandes, A. F. C.
1
; Viana, A. P. P.
1
;
Oliveira, A. S. C.
1
; Cato, R. M. R.
2

Pharmaceutical Sciences Graduate Program of State University of
Paraiba (UEPB)
2
Center of Biological and Health Sciences, Pharmacy Department, State
University of Paraiba (UEPB)
Corresponding author:cynthialayse@gmail.com

Introduction: Anadenanthera macrocarpa (AM) Benth - Fabaceae, known
s ngco e exensvey used n fok medicine for the treatment of
coughs, whooping cough, bronchitis, inflammation, healing and antioxidant.
Myracrodruon urundeuva (MU) All.-Anacardiaceae, popuy oe-do-
seo, dug use hs ncesed due o he pesence of nnns, used
against upper respiratory tract, urinary tract, hemoptysis, and metrorrhagia.
Objective: The aim of this study was to evaluate the in vitro antimicrobial
activity of hydroalcoholic extract of AM and MU, compared to strains of
Staphylococcus aureus outpatient source. Materials and Methods: Was
used as test microorganisms the standard strain S. aureus ATCC 25923,
and strains of S. aureus home care (UEPB). The study was performed by
disk diffusion method, 20L was added to extract discs with sterile filter
paper 6 mm in diameter, previously identified. The plates were incubated at
37C/24h and read thereafter. Results and Discussion: The inhibition
halos formed from the activity of the extract of AM and MU varied between
8 and 14mm. The results show that the use of medicinal plants such as
antibacterial therapy, may be quite feasible for the discovery of substances
with biological activity. Conclusion: All examined extracts showed
antibacterial activity against the strains of S. aureus tested.

Financial support: CAPES.


111

CODE 104
OUABAIN REDUCES THE PHAGOCYTOSIS OF PERITONEAL
MACROPHAGES

Leite, J. A
1
; Alves, A.K.A.
1
; JACOB, P. L.
2
; GALVO, J.G.F.N.
1
;
2
Nris, P.
L. N.;
2
Oliveira, M. R.; Rodrigues-Mascarenhas, Sandra
1

1
Centro de Biotecnologia, Laboratrio de Imunologia, Universidade Federal
da Paraba (UFPB)
2
Departamento de Biologia Molecular e Celular, Laboratrio de
Leishmaniose, Universidade Federal da Paraba (UFPB).
Corresponding author:anneabreu.farm@gmail.com

Introduction: Ouabain, a potent Na
+
, K
+
-ATPase inhibitor, was identified
as an endogenous substance of human plasma. Our group have
demonstrated the ability of ouabain to modulate inflammatory processes,
but little is known about the mechanisms involved. Phagocytosis is a
complex process, important for immune responses against pathogens and
resolution of inflammation. Objective: The aim of this work was to
investigate, in vitro, the modulating effect of ouabain on phagocytosis.
Materials and Methods: The cytotoxic effect of ouabain on peritoneal
macrophages was determined by MTT. Macrophages (2x10
5
cells/well)
were cultured in the presence of different ouabain concentrations (10nM,
100nM and 1000nM) for 24 hours. For the phagocytosis assay, cells were
also exposed to zymosan A (5x10
6
per well). Cytokine concentration was
assayed using an ELISA kit (Bio Science). Results and discussion:
Ouabain showed no cytotoxicity in all concentrations studied. However,
ouabain 100nM suppressed phagocytosis (20%), but did not affect
phagocytosis index, as well as the levels of pro- and anti-inflammatory
cytokines analyzed. Conclusions: Ouabain interferes with the phagocytic
function of macrophages. This effect is independent of IL-1, IL-6, TNF
and IL-10 release. However, further studies are necessary to elucidate the
mechanisms involved.

Financial Support: The study was conducted with financial support
from CNPq / Brazil.


112

CODE 105
EVALUATION OF THE RELAXING ACTIVITY OF ETHANOL EXTRATCT
FROM LEAVES ASPIDOSPERMA MACROCARPUM IN ISOLATED RAT
MESENTERIC ARTERY.

Oliveira M. P.
1
; Paulino, J. V. S.
1
; Aquino, P. G. V.
2
; Sant'Ana, A. E. G.
2
;
Ribeiro, . A. N.
1
; Arajo-Jnior, J. X.
2

1Escola de Enfermagem e Farmcia, UFAL, Macei/AL
2Instituto de Qumica e Biotecnologia, UFAL, Macei/AL, Brazil.

INTRODUCTION: Aspidosperma macrocarpum Mart. is a plant popularly
known s peoba-gigante-do-cedo. Seve kods hve been
isolated from the genus Aspidosperma. These alkaloids have several
pharmacological properties, such as: -adrenergic blocking activity,
hypotensive and analgesic (J. Pharm. Biom. Ana. 12:10, 1994; J. Pharm.
Sci. 62:11, 1973). In a previous work, our group has demonstrated
hypotensive activity of Aspidosperma macrocarpum in non-anesthetized
Wistar rats (Oliveira et al, 2011).OBJECTIVE: The aim of this study was to
investigate the relaxing activity of ethanolic extract Aspedosperma
macrocarpum (EEAM) in superior mesenteric artery isolated from rats
Wistar.MATERIALS AND METHODS: The plant was collected at
Planaltina (GO) in November 2007, and identified by Professor Jos E. de
Paula. The voucher specimen is deposited in the herbarium of University of
Brasilia (UnB), encoded as JEP 3767. The leaves extract was obtained by
maceration in 90% ethanol by 48 hours, and the EEAM was obtained after
removal of the solvent. From 1.6 Kg of the dry vegetal material were
obtained 517 g of EEAM. Rat superior mesenteric rings (2-4 mm) were
suspended by coon hed fo somec enson ecodngs n Tyodes
solution at 37C and gassed with a 95% O
2
and 5% CO
2
, under a resting
tension of 0.750 g. Statistical analyses were pefomed by suden es.
The study was approved by the ethics committee of the Federal University
of Alagoas (010151/2008-82). RESULTS AND DISCUSSION: In isolated
rat superior mesenteric rings, increasing concentrations of EEAM (0.1, 0.3,
1, 3, 10, 30, 100 and 300 g.ml
-1
) were able to antagonize the effects of
phenylephrine (10 M) and KCl (80 mM) induced contractions induced
contractions (EC
50
= 273,30,84 g.ml
-1
and 323,30,5 g.ml
-1
,
respectively). However, the smooth muscle relaxant activity of EEAM was
not atenuated by endothelium removal (EC
50
= 116,60,48 g.ml
-1
).
CONCLUSIONS: These results demonstrated that the non-specific
vasorelaxante activity induced by EEAM can not be attributed to the
participation of vascular endothelium. Further experiments are necessary
to elucidate the mechanisms involved.

Financial support: FAPEAL, CAPES and CNPq.


113

CODE 106
EVALUATION OF TESTS PRE-TRANSFUSIONAL IMMUNO-
HEMATOLOGICAL IN RECEPTORS OF BLOOD

Batista, M.T
1
; Ferino, L.P.P.
2
; Leal, K.D.B.

1,2,3
Academic Unit of Health, Education and Health Center, Federal
University of Campina Grande (UFCG).
Corresponding author: magnatbatista@gmail.com

INTRODUCTION: The hemotherapy in Brazil, has been characterized by
the development and adoption of new technologies with the objective at
minimizing the risks of transfusion, especially with regard to preventing the
spread of infectious and contagious agents (Carrazzone, 2004). With
regard to blood receptors, the Ministry of Health (Decree 1376/93)
determines the tests immune-hematological such as pre-transfusional
ABO / Rh classification, research irregular antibody (RIA) and compatibility
testing (crossmatch). OBJECTIVES: This study aimed to describe the
importance of immune-hematological testing pre-transfusional performed in
blood banks. MATERIAL AND METHODS: This study was compiled from
a literature review, with research in books, scientifics magazines and
scientific article. The method chosen to perform the blood type and Rh
factor was the use of "Cards", the DiaMed-ID, which is contained in a
"card" the blood type, Rh factor and reverse tests, and other "Card" RIA's
research and direct Coombs test. RESULTS AND DISCUSSION: The
points discussed in this review show that the technique using "cards" for
carrying out the immune-hematological testing has proven effective and
fast with a high level of security (Ferreira, 2010), as it was not reported in
no work the presence of immune-hematological reactions after transfusion,
showing no incompatibility for the receivers. CONCLUSION: The points
discussed in this study show that qualitative measure of immune-
hematological tests pre-transfusion taken to a safe blood transfusion have
been improved, making it more safe for the realization of the realization of
hemotherapy.


114

CODE 107
DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE LIQUID
CHROMATOGRAPHY METHOD FOR THE EVALUATION OF
RECOMBINANT HUMAN INTERLEUKIN-11. CORRELATION WITH THE
BIOASSAY

Souto R. B.
1
, Calegari G. Z.
1
, Walter M. E.
1
, Cardoso Junior C. D. A.
1
,
Stamm F. P.
2
, Dalmora S. L.
1

1
Department of Industrial Pharmacy,
2
Postgraduate Program in
Pharmaceutical Sciences, Federal University of Santa Maria, 97105-900
Santa Maria-RS, Brazil
Corresponding author: sdalmora@terra.com.br (Dalmora S. L.)

Introduction: Interleukin 11 is a multifunctional cytokine of the IL-6 type
subfamily of long-chain helical cytokines, which modulates the proliferation,
differentiation and maturation of various types of hematopoietic cells.
Recombinant human interleukin-11 produced by DNA technology is
currently being used worldwide for the prevention of thrombocytopenia and
to reduce the need for platelet transfusions after myelosuppressive
chemotherapy in patients with nonmyeloid malignancies. Objective: The
aim of this study was to develop and validate a stability-indicating
reversed-phase liquid chromatography (RP-LC) method for the
content/potency assessment of rhIL-11 and correlate the results to the
bioassay.Materials and Methods: The LC method was carried out on a
Jupiter C
4
column (250 mm x 4.6 mm i.d.), maintained at 25C. The mobile
phase A consisted of 0.1% trifluoroacetic acid (TFA) in water and mobile
phase B consisted of 0.1% TFA in acetonitrile. The gradient elution was
performed at a flow rate of 1 mL/min with UV detection at 214 nm. The
biological potency was evaluated using the bioassay based on the
proliferative activity of the TF-1 cells.Results and Discussion: The RP-LC
method validation demonstrated acceptable results for the linearity,
accuracy, precision, sensitivity, specificity and robustness, without any
interference from the excipients. Biopharmaceutical batches of rhIL-11
were analysed by the RP-LC and by the bioassay, giving potencies
between 93.23 and 103.83% of the stated potency, with significant
correlation (p>0.05). Conclusion: The RP-LC method represents an
alternative which can be used in combination with the bioassay,
contributing to improve the quality control and to assure the therapeutic
efficacy of the biotechnology-derived product.

Financial support: CNPq


115

CODE 108
STUDY OF A RP-LC METHOD FOR THE ANALYSIS OF
RECOMBINANT PARATHYROID HORMONE IN
BIOPHARMACEUTICAL FORMULATIONS

Stamm, F. P
b
, Butzge, C. S
a
, Chiossi, K.
a
, Calegari, G. Z
a
, Souto, R. B
a
,
Dalmora, S. L.
a

Department of Industrial Pharmacy
a
, Postgraduate Program in
Pharmaceutical Sciences
b
, Federal University of Santa Maria, 97105-900
Santa Maria-RS, Brazil
Corresponding author:sdalmora@terra.com.br (Dalmora SL)

Introduction: Human parathyroid hormone, hPTH, is a single-chain
polypeptide of 84 amino acids secreted by the cells of the parathyroid
glands. It is a major regulator of blood calcium levels. Recombinant human
parathyroid hormone produced by DNA technology in Escherichia coli,
consists of a 34 amino acids with a molecular mass of 41 kDa. It is
currently being used for the treatment of postmenopausal osteoporosis in
women as well as primary or hypogonadal osteoporosis in men. Objective:
The aim of this study was to develop a reversed-phase liquid
chromatography (RP-LC) method for the evaluation of rhPTH (1-34) in
biotechnology-derived products. Materials and Methods: The LC method
was carried out on a Zorbax 300SB C
18
column (150 mm x 4.6 mm i.d.),
maintained at 45C. The mobile phase consisted of 0.2 M sodium sulfate
buffer pH 2.3 and acetonitrile (65:35, V/V). The gradient elution was
performed at a flow rate of 1 mL/min with UV detection at 214 nm. Results
and Discussion: The chromatographic separation was obtained with the
retention time of 12 min and was linear over the concentration range of 10 -
300 g/mL (r
2
=0.9995).The specificity was tested showing that there was
no interference of the excipients of the formulation in the analysis. The
method was applied for the analysis of rhPTH (1-34) giving potencies
between 98.72% and 104.39%. Conclusions: The method was partially
validated and applied for the analysis of rhPTH (1-34) in biopharmaceutical
formulations, contributing to the research of alternative which improve the
quality control assuring the therapeutic efficacy of the biological medicine.

Financial support: CNPq


116

CODE 109
EFFECT OF TRIETHANOLAMINE/METHYL-1-PYRROLIDONE-
2/BETACYCLODEXTRIN MULTICOMPONENT COMPLEXES SYSTEMS
ON THE BENZNIDAZOLE APPARENT SOLUBILITY

Melo, P. N.
1
; Rocha, H. C. S.
2
; Silva-Junior, A. A.
1

1
Graduate Program on Pharmaceutical Sciences, Federal University of
Rio Grande do Norte (UFRN), Natal RN, Brazil.
2
Laboratory of Pharmaceutical Technology and Biotechnology, Department
of Pharmacy, UFRN, Natal RN, Brazil.
Corresponding author:polyanne_melo86@yahoo.com.br

Introduction: Benznidazole is a poor soluble drug and the only available
for Chagas disease treatment in Brazil. Tthe increment on the its aqueous
solubility is an interesting alternative. Objectives: The purpose of this
study was to check the influence of betacyclodexn (-CD),
triethanolamine (TEA) and methyl-1-pyrrolidone-2 (MP), and to establish
the cosolvency or complexant mechanism of both TEA and MP in change
of BNZ aqueous solubility. Materials and methods: The solubility studies
were performed using the phase solubility diagrams described by Higushi
nd Connos (1965). The nfuence of he ssocon of -CD (0-0,015
mol.l
-1
) with TEA (10%) and MP (10%) was also investigated. The
cosolvency or complexing mechanism was elucidated using the Jain and
Yalkowsky method (2007), in which the the cosolvency occurs when a
semi log plot Sw (solubility in water)/Smix (solubility in the co-solvent
solution) versus fraction of solubilizer (f) (TEA or MP) is linear. Results
and discussion: The increment on the BZN solubility was about 1.72; 4.9
and 28 fold for -CD, TEA, and MP, respectively. The association of TEA
or MP with -CD lead to increase of 1.56 (-CD 0-0,015 mol.l
-1
+ TEA
10%) and 3 times (-CD 0-0,015 mol.l
-1
+ MP 10%). It was identified a
consolvency mechanism for TEA, while MP presented also a complexant
effect. Conclusion : TEA and MP increase the BNZ solubility significantly.
However, the association with TEA and MP did not show a synergic effect.

Financial support from CNPq/Brazil CNPq


117

CODE 111.1
PHYSICAL PARAMETERS OF QUALITY ASSESSMENT OF
DIFFERENT LOTS OF HOMEOPATHIC GLOBULES IN USE IN NATAL
/RN/BRAZIL

Cab, C. M.; Queiroz, A.; Navarro, M. V. M.; Arago, C. F. S.

Quality Control of Drugs Laboratory LCQMed Pharmacy Department
Federal University of Rio Grande do Norte - Brazil

Introduction: Homeopathic globules are obtained industrially from sucrose
or mixture of sucrose and lactose. The composition of them may be
affected by factors such as temperature and humidity. Moreover, the
method used for impregnation can have an effect on factors such as dose
uniformity and weight of the globules. Objective: This study aims to
evaluate the quality of globules used in the preparation of homeopathic
products, used in Natal/RN/Brazil. Material and methods: Homeopathic
globules, inert and impregnated with EtOH70%, in three different lots were
assessed by determining the physical parameters. The friability test was
carried out by friabilator and rotated 100 times (25 rpm for 4 min). The
hardness test was carried out by using two different hardness tester
(manual and automatic tester). The weight uniformity test was carried out
as follows: 20, 50 e 100 globules from each brand were selected, dusted
and weighed individually.Adequate globules hardness and resistance to
powdering and friability are of paramount importance for consumer. Recent
studies have explained relationship that exists between globules hardness
and its disintegration and more significantly drug dissolution. Results and
discussion: Regarding theweight, no significant changes were observed in
inertlots, compared to impregnated withEtOH70%. No significant changes
were observed for friability tests in inert and impregnated globules. The
hardness test showed a decrease in values observed in impregnated as
compared toinert globules, demonstrating a possible reduction of the
hardness due to the presence of the vehicle.


118

CODE 111.2
SPECTRAL ANALYSIS (FT-IR, UV) OF RELEASE OF LEACHABLES
FROM NATURAL RUBBER BULBS

Cab, C. M.; Navarro, M. V. M.; Arago, C. F. S.

Quality Control of Drugs Laboratory LCQMed Pharmacy Department
Federal University of Rio Grande do Norte - Brazil

Introduction: Chemical bonds vibrate at specific frequencies, which are
determined by the mass of the constituent atoms, the shape of the
molecule, the stiffness of the bonds, and the periods of the associated
vibrational coupling. Objective: In this study, we realized spectral analysis
(FT-IR, UV) of release of leachables from natural rubber bulbs by factorial
design. Material and methods: Natural rubber bulbs were analyzed with
infrared and UV spectrometry. Rubber bulbs from West
samples were
prepared with the help of factorial design.90mgof the bulbs were extracted
with 10ml of different solvents (chloroform, n-hexane, acetone and ethanol-
30, 70 and 100%). Results and discussion: The extractive solutions
showed characteristics transitions of possible transfer of materials: natural
rubber was characterized by the vibrations of2920 (m) and 2852(F), (vCH),
3437(F), (vOH), 1617 (m) (oC=C) and 1384 (F), (oC=O). The bands
corresponding to the presence of the accelerator were found in 3557 (F)
and 3406 (F) (vNH). The bands found in 3412 (F) (vS=O) corresponds to
the vulcanizing agent. Conclusions: According to data obtained by UV
spectrometry, the samples followed the order of release of constituents of
the rubber in the following order: CHCl
3
>acetone> n-hex>EtOH>70%
EtOH>30% EtOH.


119

CODE 112
TOXICITY OF DENTAL VARNISH CHITOSAN

Cavalcante, H. M. M.
1,3
; Lidiane Pinto Correia
1
; Thayza Christina
Montenegro Stamford
1
; Sergio Luiz Dalmora
2
; Rui Oliveira Macdo
1

1
Department of Pharmaceutical Sciences, Unified Development
Laboratories and Essays of Drugs, Federal University of Paraba,
2
Department of Industrial Pharmacy, Federal University of Santa Maria,
3
Nucleus of Health Research of Integrated Colleges of Patos- Brazil
Corresponding author:horacinna@yahoo.com.br

Introduction:Chitosan, a cationic amino polysacchde, esseny -1,4
D-glucosamine (GlcNAc) linked to N-acetyl-D-glucosamine residues, is
naturally present in the cell wall of certain fungi, and can also be obtained
by chitin deacetylation. Objective: The objective of this study was to
evaluate the toxicity of dental varnish chitosan in chorioalllantoic membrane,
NCTCclone929 and CHO cell culture. Material and methods:The toxicity
of the dental varnish chitosan was evaluated inchorioallantoic membrane of
chick embryo at 10
th
day of incubation. The eggs were obtained from
GuaravesGuarabira Aves Ltda and incubated at 37C.
Thecytotoxicitystudieswere conductedbyneutral reduptake
assayusingNCTCclone929 and CHO cell culture. Results and
discussion:The dental varnish chitosan evaluated inchorioallantoic
membrane promotedvasoconstriction, coagulation and bleedingwhich was
initiated at the following time (seconds) 133 7.35, 205 4.97 and 169
5.72,respectively.The DL
50
values obtained for dental varnish chitosan in
NCTCclone929 and CHO cell culture were: 0.2064 mg ml
-1
and 0.3691,
respectively.Conclusions:The dental varnish chitosan was shown to be
irritating inchorioallantoic membrane. The results of cytotocity corroborate
with the of chorioallantoic membrane.
.



120

CODE 113
NEW TALENT PROGRAM EDUCATION FOR HEALTH ANALYSIS
AND EDUCATION IN WATER USE IN THE OF TRAIPU ALAGOAS.

Silva Jnior, E. F.; Reys, J. R. M.; Trindade
1
, R. F. C; Nascimento
1
, T. G.;
Silva, M. E. B
2
.; Silva, A. T.; Souza, R. S
1
.; Mbambi, S. J. T
1
.; Amado, A.
P.
2
; Silva, I. M.
1
; Silva, P. H. V. M
1
.

1
Escola de Enfermagem e Farmcia, Coordenador do Curso de Farmcia,
Universidade Federal de Alagoas UFAL
2
Faculdade de Medicina, Universidade Federal de Alagoas UFAL
3
Centro de Tecnologia, Engenharia-Qumica, Universidade Federal de
Alagoas UFAL
Corresponding author: edeildojr@hotmail.com

Introduction: With actual greats problems involve the Water in several
parts of the world, become each time more essential to work the question
of the conscience of people of one shape general. That work was realized
in Traip AL, where it appears on second place on the raking of cities
with worse IDH of country. Materials and Methods: The work was
accomplished with 30 children and 30 teachers, both of public and
municipal schools. Were used banners for exposition of pictures related
wh he wes wse, pouon, dseses nd ohes; compues fo
presentation of documents pptx; books for to help on building of
educational dramatizon bou good pcces fo vod he wes
waste. Results and Discussion: Was clear the city have biggest needs
wh eon he quesons of pocs of weness fo vod he wes
waste and pollution. There was, after the realize works, had a major
advance on question educative referent the water. Conclusions: The work
was an awesome way of awareness for the target-public, leading a level of
understands major about the discussions involving in Water. But, know
there more for to make, because is a continuous work and can expand for
other regions.

CAPES/Brazil.


121

CODE 114
EVALUATION OF THE CARDIOVASULAR RISK IN CONNECTION TO
C-REACTIVE PROTEIN

Ramos, K. R. L. P.; Silva, J. R. S.
2
; Muniz, T. T. M.
2
; Sousa, A. P. B.
2
;
Ramos, R. A.
2
; Rocha, W. R. V.
2
; Peixoto, J. B. S.
3
; Guimares, N. E. S.
4
;
Clementino, F. R. B.
4
; Cunha, M. A. L.
5
.

Paraiba (UEPB)
2
Pharmaceutical Degree of State University of Paraiba (UEPB)
3
Public health Graduate Program of State University of Paraiba (UEPB)
4
Lab technician of State University of Paraiba (UEPB)
5
University of Paraiba (UEPB)
Corresponding author katharinaporto@gmail.com

Introduction: The C-Reactive Protein (CRP) is an acute phase protein,
synthesized by the liver in answer to cytokines, that reflect active systemic
inflammation constituting an inflammatory marker considered as a strong
predictor independent of risk for a cardiovascular event. Objective: The
analysis of the us-CRP in patients assisted in a Basic Unit of Family
Health. Methodology: The study was of the transversal type, documental,
descriptive and analytical whose data was analysed statistically through
the Epi-Info version 3.4. Between august of 2010 and august of 2011. The
biochemical determinations were made in the Metrolab 2300 and the us-
CRP was dosed by chemiluminescence in automated equipment
IMMULITE 1000. Results and Discussion: The sample was constituted of
104 patients, being 18.3% of the male genre and 81.7% female, of which
48.1% correspond to the age group of 60-80 years. It was observed that
35.5% of the patients were hypertensive and had type 2 diabetes mellitus,
of these, 25% were classified in accordance to us-CRP as high risk (p<
0.009). After analysis it was observed that 42% presented elevated
cholesterol with 24% of high risk for us-CRP. As for the HDL-cholesterol,
82.7% of the patients showed altered values, being 47.1% with high risk for
us-CRP. Conclusion: A great relevance was observed of the research of
us-CRP as a way of identifying the subclinical inflammation what will
contribute to the preventive strategies aiming the reduction of risk factors
that contribute to the development of Cardiovascular Diseases.



122

CODE 115
CHEMICAL PROFILE VERIFICATION OF THE EXTRACTS AND SEMI-
PURIFIED FRACTIONS of Andira nitida Mart. ex Benth BY TLC

Ferreira, A. V. D, Moraes, M. A.; Fonseca, B. A. D.; Guia, F. C.,
Monteiro, D. K.; Moreira, G. A., Santana, M. C.; Machado, A. K. M. S.;
Silva, A. E. G.; Simas, N. K., Zanetti, G. D., Konno, T. U.; Leite, M. N.;
Rocha, F. D.
2,3

1
Mestrando em Cincias Farmacuticas, UFJF,
2
Laboratrio de Farmacognosia, Faculdade de Farmcia, Universidade
Federal de Juiz de Fora (UFJF).
3
Faculdade de Farmcia, Universidade Federal do Rio de Janeiro (UFRJ-
Maca).
Corresponding author antonioviniciusdf@gmail.com.

Introduction: Andira nitida (Fabaceae) is part of the plants popularly
known as Angelins, which are species rich in isoflavones, flavanols,
rotenoids. Objective: Evaluating the chemical profile of the A. nitida
branches Hex / DCM 8:2 and EtOH: H2O 8:2 extracts, as well as semi-
purified fractions. Material and Methods: The botanical material was
collected at restinga of Jurubatiba in Quissam-RJ, Brazil. After drying and
grinding, 1.2 kg of material was extracted consecutively with Hex / DCM
8:2 and EtOH: H2O 8:2. The lyophilized obtained from hydroalcoholic
extract was purified with solvents of increasing polarity (hexane, CHCl
3
,
EtOAc, BuOH) on ultrasound. For CCD profiles verification, 10mg/mL
solutions were prepared from dry crude extracts and semi-purified
fractions. Solutions (5mg/mL) of eugenol, coumarin apigenin, standard
steroid, quercetin, sparteine, terpene standard, rutin, tannic acid, coumaric
acid and catechin were used as standards of comparison. 5L were
applied on silica gel plates. After development with several eluent systems,
the dry plates were visualized under UV light (254 and 366nm), then they
were reveled with ceric sulfate, anisaldehyde sulfuric acid, vanillin sulfuric
acid, 10% FeCl
3
in MeOH, Dragendorff, NP-PEG, KOH 1N, 0.2% DPPH in
MeOH. Results and Discussion: Compounds as terpenes, steroids and
phenolic derivatives (especially flavonoids and tannins) were identified. In
addition, some substances with antioxidant potential in the hydroalcoholic
extract and their fractions could be visualized. Conclusion: The conditions
for analysis by TLC showed adequated for identification of secondary
metabolites classes present in organic extracts and their fractions. These
results are consistent with the literature.

Financial support from FAPERJ, CNPq, PROPG


123

CODE 116
PRO-INFLAMMATORY AND CYTOTOXIC ACTION CARRAGEENAN OF
THE SEAWEED AMANSIA MULTIFIDA

Souza, L. A. R.*; Cruz, A. K. M. *; Leite, E. L. *

1
Laboratory of vegetal Glicobiology, Department of Biochemistry, Federal
University of Rio Grande North (UFRN)

Introduction. Carrageenan are sulfated polysaccharides present in cell
walls of red algae and well known in the food industry as gelling and to
induce inflammation process in animal model. Objective. This work aims
to evaluate the pro-inflammatory and cytotoxic effects of polysaccharide
fraction extracted from red algae Amansia multifida. Materials and
Methods. The pro-inflammatory effects in vitro were determined by the
ability of fractions cause hemolysis by the alternative pathway
of complement system (CS) and direct cell hemolysis (10, 25 and 50 g of
sample) and in vivo by testing acute peritonitis in swiss mice (n =5),
determining the amount of leukocytes and the interleukin-6 (IL-
6) concentration of the peritoneal cavity (dose of 25 and 50 mg/kg of body
weight (BW)). The in vitro cytotoxicity was performed
using mouse fibroblast cells (3T3), evaluated by MTT method,
with incubation time of 24 and 48 h (10, 50 and 100 g of sample). Results
and Discussion The fraction had no directly lysis effect on red blood
cell tested in the masses, however, caused stimulation of up to 42.6%
(50 g) (p<0.001) of hemolysis by the alternative pathway of the CS.
The fraction promoted an increasing of 313.8% (25 mg/kg BW)
and 670.1% (50 mg/kg BW) in cell migration when compared to group
treated with saline (p<0.001). The dosage of IL-6 showed values of
45.8 pg/mL (25 mg/kg BW) and 120.2 pg/mL (50 mg/kg BW). Conclusions
Wasnt observed a significant cytotoxic effect of 3T3 cells treated with the
fraction in 24 h of incubation. However, with 48h observed cell death of
79% (100 g) (p<0.001). The results in question on the use
of carbohydrates as additives in foods, cosmetics and medicines.

Financial supported by CNPq/Brazil


124

CODE 117.1
ANTIFUNGAL ACTIVITY OF MEDICINAL PLANTS AGAINST CANDIDA
SPECIES

Guimares, G. P.
1
; Santos, R. L.
2
; Medeiros, A. C. D.
2
; Vieira, K. V. M.
3
.

1
Campina Grande-PB-Brazil.
2
UEPB, PPGCF, Laboratrio de Desenvolvimento e Ensaios de
Medicamentos (LABDEM), Campina Grande-PB-Brazil.
3
UEPB, Departamento de Farmcia, Laboratrio de Microbiologia,
Corresponding author geovanipguimaraes@gmail.com

Introduction: The use of medicinal plants has been widely seen as basis
for health maintenance. It is commonly found in female disorders such as
oral and vulvovaginal candidiasis caused by Candida species. The
increasing resistance to usual antifungal agents demands for research on
new sources, such as medicinal plants. Objectives: To evaluate the
antifungal activity of medicinal plant against the main Candida species
related to oral and vulvovaginal candidiasis. Materials and Methods: Was
evaluated the antifungal activity of hydroalcoholic extract (70%) made from
medcn pns used n Bzn fok medcne: fvc-de-cboco
(Hyptis Mutablis Bq.), cum (Annona coriacea L.), mex (Ximenia
americana L.), bn (Schinopsis brasiliensis Eng.) nd cpcho-de-
cgno (Acanthospermum hispidum DC.). The test was performed against
the species Candida albicans (ATCC 18804), C. guilliermondii (ATCC
6260), C. krusei (ATCC 34135), C. parapsilosis (ATCC 22019) and C.
tropicalis (ATCC 13803) using the agar diffusion method with cylinders.
The results were expressed as the average diameter of the growth
inhibition zone around the cylinders. The experiments were performed in
triplicate. Results and Discussion: The extract of brana (Schinopsis
brasiliensis Engl.) was active against the tested species, and minimum
inhibitory concentration (MIC) at a dilution of 25 and 50% to
C.guilliermondii and C. parapsilosis respectively. The extracts from other
plants has not shown activity against the yeasts. Conclusions: The leaf
extract of brana can be further studied and the extract or her (s) active (s)
possibly can be used to obtaining alternative drugs against vulvovaginal
and oral candidiasis caused by these Candida species.

Financial support from CNPq-PIBIC/UEPB.


125

CODE 117.2
DEVELOPMENT OF A LSVM-5CN05 TRANSDERMIC
MICROEMULSION

Guimares, G.P.
1
; Mendona-Jr, F.J.B.
1,2
, Damasceno, B. P. G. L.
1
, Silva,
J.A.
1

1
2
UEPB, PPGCF, Laboratrio de Sntese e Vetorizao de Molculas
(LSVM), Joo Pessoa-PB-Brazil.
Corresponding author geovanipguimaraes@gmail.com

Introduction:Thiophenes derivatives (TD) are strongly insoluble molecules
with several biological activities, especially against fungal species of
genus Cndd nd Aspegus. Seve TD wee synheszed by UEPBs
research group, like 2-[(3,4-dichloro-benzylidene)-amino]-5.6-dihydro-4H-
ciclopen-ta[b]thiophene-3-carbonitrile (LSVM-5CN05). Microemulsions
(ME) are defined as systems thermodynamically stable and optically
transparent formed from a mixture of two immiscible liquids stabilized by an
interfacial film of surfactants. These systems can be aimed to develop
therapeutic agents based on drugs hardly incorporated due to their high
lipophilicity, such as LSVM-5CN05. Objective: To develop and to
characterize a LSVM-5CN05-ME. Material and Methods: The
microemulsion was prepared by a mixture of Labrasol (S), Plurol
Oleique (CS), isopropyl myristate (O) and distilled water (W). The LSVM-
5CN05-ME was obtained by adding the drug to the mix of components S,
CS and O by stirring in Ultra-Turrax T25 at 9000 rpm for 3 minutes. Then,
the distilled water was added to this mixture, followed by stirring and ultra-
sound bath and the macroscopic aspect, refractive index and pH were
analyzed after 48 hours. Results and Discussion: The preparation of the
LSVM-5CN05-ME at 400g mL
-1
result in a system clear, isotropic and
translucent, with the refractive index values of 1.410 (blank ME) and 1.412
(LSVM-5CN05-ME). The pH values were 4.100.04 and 4.180.02 to blank
ME and LSVM-5CN05-ME, respectively. Conclusions: This ME system
appears to be a good delivery system to theLSVM-5CN05 and other
aspects will be investigated for better analysis of the system.

Financial support from PROPESQ/UEPB.


126

CODE 118
ACUTE AND SUBACUTE TOXICITY OF Caesalpinia ferrea STEM
BARK EXTRACT

Vasconcelos, C.F.B.
1
; Maranho, H.M.L.
1
; Menezes, M.H.G.
2
; Limeira,
M.M.F.
2
; Lima, C.R.
1
; Dimech, G.S.
2
; Gonalves, E.S.
1
; Costa-Silva, J.H.
3
,
Wanderley, A.G.
1,2
.
1
Department of Pharmaceutical Sciences,
2
Department of Physiology and Pharmacology,
3
Department of Physical
Education and Sport Sciences, UFPE, Brazil.

Introduction: Caesalpinia ferrea Martius (Leguminosae) has been used
for the treatment of diabetes, however there are no toxicological studies
about its safety use. Objectives: To evaluate acute and subacute toxicity
of aqueous extract of stem bark of C. ferrea on hematological parameters
of Wistar rats. Materials and Methods: In acute test, the extract was
administered at dose of 2000 mg/kg for both sexes (n=5/group/sex) and
changes in body weight, behavior, warning signs and mortality were
observed for 14 days. In subacute test, animals were treated orally with
vehicle (control group, C) or aqueous extract of stem bark of C. ferrea in
doses of 300 (T1) or 1500 (T2) mg/kg/day for 30 days. At the end of the
treatment, animals were anesthetized with Nembutal (35 mg/kg, ip) and
blood samples were collected for hematological analysis. Results and
Discussion: Acute dose of C. ferrea 2000 mg/kg produced no signs of
toxicity or death in rats. In subacute test, no deaths or toxic signs were
observed. In addition, there were no changes in hematological parameters:
Eritrocytes (C=8.430.13, T1=8.520.19 and T2=8.420.20 10
6
/mm
3
),
hemoglobin(C=16.070.34,T1=16.290.43 and T2=15.880.39 g/dL),
hematocrit (C=47.200.92, T1=47.471.12 and T2=46.111.04%), platelet
count (C=750.4028.71, T1=716.9031.29 and T2=657.8034.07
10
3
/mm
3
), leucocytes (C=16.010.99, T1=11.460.78 and T2=15.411.16
10/mm), neutrophils (C=10.430.85,T1=10.470.83 and T2=11.15
0.81%), eosinophils (C=0.890.31, T1=0.260.22 and T2=0.190.18 %),
basophils (C=0.560.05,T1=0.440.08 and T2=0.350.04%), lynfocytes
(C=84.851.13, T1=85.171.04 and T2=83.321.44 %) and monocytes
(C=3,270.39, T1=3.660.35 and T2=4.990.87 %). Conclusions: The
acute and subacute oral administration of aqueous extract of stem bark of
C. ferrea presented no toxicity in Wistar rats.


127

CODE 119.1
ANTINOCICEPTIVE ACTIVITY OF CURINE ON THE ACETIC ACID
ABDOMINAL CONSTRICTION TEST (WRITHING TEST) IN MICE

Salgado, P. R. R.
1
; Leite, F. C.
1
; Fonsca, D. V.
1
; Piuvezam, M. R.
1
;
Almeida, R. N.
1

1
Laboratrio de Psicofarmacologia, Centro de Cincias da Sade,
Universidade Federal da Paraba (UFPB)

Introduction: Curine is the major structural bisbenzylisoquinoline alkaloids
that is isolated from the root barks of Chondrondendron platyphyllum, a
medicinal plant from the northwest region of Brazil belonging to the
Menispermaceae family. It has been reported on literature that some
species from this family exert activity on central nervous system (CNS).
Objective: This study aims to investigate the possible antinociceptive
effects of curine through the acetic acid abdominal constriction test
(writhing test) in mice. Material and Methods: Swiss male mice were
used, 25-35 grams, 3 months old (N=8). The negative control group
received distilled water and tween 80; the experimental group was treated
with curine (2.5 mg/kg) and positive control, morphine (10 mg/kg; i.p.). In
the writhing test, the animals were treated with acetic acid 1.0%, and then
observed the latency to the onset of the first contortion and the total
number of contortions displayed by each animal. Results and
Discussion: Treatment with curine (2.5 mg/kg) induced a significant
increase in the latency to the onset of the first contortion compared to the
control group (267.210.9 vs 217.710.8) and induced a significant
reduction in the number of contortions (35.42.2) compared to the control
group (47.81.3). These results show that curine (2.5 mg/kg) has, at least
preliminarily, antinociceptive activity, demonstrated by the writhing test.
Conclusions: These results suggest that the curine has antinociceptive
activity. Therefore, more studies are needed to determine whether the
antinociceptive activity of the type reported is central, peripheral or
involving the two pathways.

Financial support: CNPq.


128

CODE 119.2
EVALUATION OF THE ANXIOLYTIC EFFECT OF ETHANOLIC
EXTRACT OF Sidastrum micranthum (MALVACEAE) ON THE
ELEVATED PLUS-MAZE TEST IN MICE.

Salgado, P. R. R.
1
; Carvalho, F. L.
1
; Torres, P. A.
1
; Pereira, W. B.
1
; Gomes,
R. A.
1
; Almeida, R. N.
1
; Morais, L.C.S.L.
1

1
Universidade Federal da Paraba (UFPB)

Introduction: It is estimated that about 25% of all modern medicines are
directly or indirectly derived from plants. The object of this paper is the
extract of Sidastrum micrathum, a species of the family Malvaceae,
unprecedented with regard to pharmacological studies. Objective:
Evaluate whether Sidastrum micranthum produces a pharmacological
action similar to anxiolytic drugs using the elevated plus-maze method in
mice. Matherials and Methods: Swiss male mice were used, 25-35
grams, 3 months old (N=8). The negative control group received distilled
water and Tween 80, the experimental group were treated with ethanolic
extract of the Sidastrum micranthum (250 mg/kg) and positive control,
diazepam (0.5 mg/kg). All administrations were performed intraperitoneally.
After treatments, the animals were submitted to the elevated plus maze
apparatus for 5 minutes. Results and Discussion: The ethanolic extract of
Sidastrum micranthum (250 mg/kg) showed no significant changes in
compared to control group in the different parameters observed, such as:
number of visits to the open and closed arms (3.10.9 vs 2.30.9; 6.60.9
vs 5.51.1, respectively) and time spent in open and closed arms (20.95.9
vs 18.57.6; 199.826.0 vs. 246.117.9, respectively). The results show
that the extract in question, despite having CNS depressant effects
demonstrated in previous studies, has not anxiolytic drug profile.
Conclusions: These data suggest that the ethanolic extract of Sidastrum
micranthum not change the exploratory ability of animals in the elevated
plus-maze testing, might not have anxiolytic activity, which is still a
previous report, requiring further studies to validate it.

Financial support: FAPESQ.


129

CODE 122
SCANNING ELECTRON MICROSCOPY (SEM) / ENERGY-
DISPERSIVE X-RAY SPECTROSCOPY (EDS) AS AN METHOD FOR
THE CHARACTERIZATION OF MICROPARTICLES.

Lima Neto, S. A. de
2
, Batista, C. C. N.
2
, Vargas, M. R. W. de
3
, Torres, S.
M.
4
, Souza, F. S. de
2
, Oliveira, E. de J.
1

1
Laboratrio de Tecnologia Farmacutica, Departamento de Cincias
Farmacuticas, Universidade Federal da Paraba (UFPB).
2
Laboratrios Unificados de Desenvolvimento e Ensaios de
Medicamentos, Departamento de Cincias Farmacuticas,
Universidade Federal da Paraba (UFPB).
3
Laboratrio de Desenvolvimento de Medicamentos. Departamento de
Farmcia, Universidade Federal do Rio Grande do Norte (UFRN).
4
Departamento de Tecnologia Mecnica, Centro de Tecnologia,
Corresponding author: severino.lima37@gmail.com

Introduction. Solid dispersions (SD) are of great current interest as a
formulation strategy. This is because if can be used to increase the
effective solubility and bioavailability of insoluble active pharmaceutical
ingredients. This work was aimed to characterize SD of glibenclamide
(GB) with Polyethylene glycol (PEG1500/6000) and Colloidal silicon
dioxide (AER) obtained by spray drier (SDy). Objective. Characterization
of microparticles obtained by spray drier by SEM / EDS. Materials and
Methods. Solid dispersion (GB+AER+PEG1500/6000) were analyzed by
SEM recorded in a LEO1430, using a voltage of 10 or 20 kV
(backscattered electron (BSE) image), after they have been gold
sputtered. EDS spectroscopy image (dot-mapping) analysis of SD, was
done by compressing the SD into a cellulose matrix (120 mg cellulose :
80 mg SD) and pressed at 8,0 tons. The resulting tablet was polished
with sandpaper of three different grades (down to 1 micron), after what it
was gold sputtered and analyzed. Results and Discussion. A complete
and homogeneous dispersion of the active pharmaceutical ingredient
(GB), represented by the elements chlorine, nitrogen, sulfur and silicon in
the tablet was observed by EDS-dot-mapping. The distribution of
characteristic elements of the GB is evidently seen as homogenous. The
same cannot be observed with respect to GB obtained by others
techniques. Conclusions. SEM / EDS was found to be a valuable tool in
the evaluation and characterization of microparticles. The resolution of
images obtained by EDS, provide a detailed observation of the
morphology of the inclusions, compositional qualitative in this type of
sample preparation.

Financial support from CAPES/CNPQ/Brazil.


130

CODE 123
CLINICAL MODELS AND EVALUATION MEASURES IN THE STUDY OF
EXPERIMENTAL ANXIETY

Chaves neto, G
1
; Almeida, RN
3; 4
; Arago, RF
1
; Braga,

JEF
2
; Trajano,
FMP
1
.

1
Graduating in Nursing from the Federal University of Paraba.

2
Department of Public Health Nursing and Psychiatry of the Federal
University of Paraba.
3
Departament of Physiology and Pathology of the Federal University of
Paraba.
4
Lab of Pharmaceutical Technology of the Federal University of Paraba.
Corresponding author: gabrielchavesufpb@hotmail.com

Introduction. Anxiety is a universal experience characterized as a human
emotional state like love, hate and blame. It is composed by
emotional/behavioral and physiological factors. The discovery of drugs with
therapeutic potential for anxiety in its pathological manifestation involves
the development of scientific researches that use animal and human
models of anxiety. In order to evaluate elicited anxiety in the human
models for anxiety induction, it is commonly used psychological and/or
physiological measures. The psychological measures implicate the use of
psychometric scales and the physiological ones the measurement of
parameters that reflect the organic changes produced or resulting from the
elicited state of anxiety. Objective.The following review aims to present
accumulated evidence in the literature around the main human models for
induction of anxiety and the measures frequently used for its evaluation.
Materials and Methods It was made a bibliographic research having as
source of research articles listed by Pubmed and Medline databases and
research in specific books which have investigated the main human
models for experimental anxiety induction. Both the psychological and
chemical models for anxiety induction present ethical limits and are
constituted of fundamental links between basic research and therapeutic
tests done with the patients. The psychological and/or physiological
measures for evaluating experimentally produced anxiety reflect body
changes that express the level of the elicited anxiety. Conclusions.The
use of clinical models and evaluation measures in the study of
experimental anxiety makes possible, under the scientific research,
understanding the neurobiological substrate of anxiety in its pathological
manifestation, beyond evaluating drugs with anxyolitic potential.


131

CODE 125
EVALUATION OF THE TYPE ACTION ANTIDEPRESSANT OF THE
EXTRACT HYDROALCOHOLIC OF Eupatorium ayapana Vent.

MELO, A.S.; CARVALHO, S
2
.; SILVA, J. B
2
.;BAETAS
3
, A. C.;SOUZA,
P.J.C
3
; MAIA, C.S.F
3

1
Student of the Post-Graduate Pharmaceutical Sciences, College of
Pharmacy, University Federal of Par (UFPA). pharmamelo@gmail.com
2
Student of the Post-Graduate Pharmaceutical Sciences, College of
Pharmacy, University Federal of Par (UFPA).
3
Professor of program in Pharmaceutical Sciences, College of Pharmacy,
University Federal of Par (UFPA).

Introduction: The Eupatorium ayapana Vent., popularly known as japana is
used in infusions, decoctions, like sedative, to combat insomnia, headache,
etc.. Is widely used by the Amazonian population. Objective: The objective
of this study was to evaluate the behavioral effects in the SNC, the
hydroalcoholic extract (EHAEA) of the leaves and stems of Eupatorium
ayapana Vent. Materials and Methods: This study evaluated the action of
different doses of EHAEA on the behavior of Wistar rats, aged 2 months.
We used eight groups of rats (n = 7) were divided into control (saline 0.9%
ml/kg), anxiolytic action of standard drug (diazepam 1 mg/kg), standard
drug action antidepressant (fluoxetine 10 mg / Kg) and 5 doses of the
extract (100, 200, 400, 600, 800 mg / kg) . The administration was by oral.
The behavioral test was the open field, the elevated plus-maze and forced
swimming. To perform the experiment, animals were keept 1 hour before
testing at the Laboratory of Experimental Pharmacology / UFPA, where
testing was performed to acclimatization of the animals. Results and
Discussion: The results in the open field test showed decreased locomotor
activity of animals when compared with the control group. In the elevated
plus-maze, the extract did not show anxiolytic activity in the forced swim
and there was a reduction in immobility time compared to control,
characterizing a type of antidepressant action of the extract. Conclusions:
With these results, this paper aims to contribute to future studies,that can
strengthen the use of extract of Japana in folk medicine.

Financial support from Fapespa /Brazil


132

CODE 126
EXTRACTION, MEASUREMENT AND ANALYSIS OF TOTAL PROTEIN
OF NATIVE PLANTS FROM RN

Menezes, Y. A. S.
1
1
1
; Uchoa, A.
F.
2
; Pedrosa, M. F. F.
1
*

1
Laboratrio de Tecnologia e Biotecnologia Farmacutica, Departamento
de Farmcia, Universidade Federal do Rio Grande do Norte (UFRN)
2
Laboratrio de Qumica de Protenas, Departamento de Bioqumica,
Corresponding author: pedrosa@ufrnet.br

Introduction: In recent years the discovery of bioactive peptides increased
exponentially. Noting the great potential that the plant kingdom presents as
a source for new drugs, the extraction of compounds in plants has been an
important source of identification of molecules with pharmaceutical
properties. Objective: This study aims to isolate and characterize plant
proteins with antimicrobial, anti-venom and anti-inflammatory activities.
Materials and Methods: Samples of plant tissue Acmella uliginosa,
Chenopodium ambrosioides, Euphorbia tirucalli, Jatropha molissima and
Cnidoscolus urens L. were fragmented and macerated in liquid nitrogen.
The extraction was performed in Tris-HCl 0.05 M NaCl 0.15 M, pH 7.5, at
ratio of 1:2 (w / v) for 2 hours at 4 C while stirring. The extracts were
evaluated by UV spectrophotometry at 280nm, measured by the BCA
method, and evaluated by SDS-PAGE denaturing gel to 12%. The crude
extract was fractionated by precipitation with acetone at 0.5, 1.0 and 2.0
volumes. Results and discussion: In the evaluation of protein content by
spectrophotometry and measurement by BCA, we found that samples of
leaves of C. urens (UV/280nm: 29605.44g/mL and BCA: 15369.7g/mL)
and J. molissima (UV/280nm: 18775.51g/mL and BCA: 9384.52g/mL)
stood out in the two methods applied. When analyzed by SDS-PAGE, the
profile has changed, where the samples C. ambrosioides (sheet: 15 bands;
root: 14 bands) and C. urens (sheet: 6 bands) stood out. Conclusion:
Samples of C. ambrosioides and C. urens reported excellent results for the
presence of proteins. The next stage of this work is testing of biological
activity.

Financial Support: CNPq/Brazil.


133

CODE 128
REPEAT-DOSE TOXICITY OF THE AQUEOUS EXTRACT OF
Caesalpinia ferrea STEM BARK

Vasconcelos, C.F.B.
1
; Maranho, H.M.L.
1
; Limeira, M.M.F.
2
; Menezes,
M.H.G.
2
; Lima, C.R.
1
; Dimech, G.S.
2
; Gonalves, E.S.
1
; Costa-Silva, J.H.
3
,
Wanderley, A.G.
1,2

1
Department of Pharmaceutical Sciences,
2
Department of Physiology and Pharmacology,

3
Department of Physical Education and Sport Sciences, UFPE, Brazil.

Introduction: Caesalpinia ferrea Martius(Leguminosae) has been used in
Brazilian traditional medicine, especially to treat diabetes. Objective: To
evaluate the subacute toxicity (30days) of the aqueous extract of the stem
bark of C. ferrea on biochemical parameters of rats. Materials and
Methods: Male Wistar rats (280300g) were divided into three groups
(n=10/group). Animals were orally treated with vehicle (control group, C)
and the aqueous extract of stem bark of C. ferrea at doses of 300(T1) and
1500(T2) mg/kg/day for 30 days. At the end of the treatment, animals were
anesthetized with Nembutal (35 mg/kg,i.p.), and blood samples were
obtained for biochemical studies. Results and Discussion: No toxicity
signs or deaths were recorded during 30 days of oral treatment with C.
ferrea 300 and 1500 mg/kg. In biochemical analysis, it was observed an
increase (*p<0.05) in amylase levels (C=498.2033.22; T1=607.4022.36*;
T2=664.3039.55* U/L), but no differences were observed in glucose
(C=92.896.12; T1=98.783.52; T2=92.902.04 mg/dL); blood urea
nitrogen (C=33.441.16; T1=29.561.65; T2=31.401.45 mg/dL);
creatinine (C=0.710.03; T1=0.700.02; T2=0.650.02 mg/dL); aspartate
aminotransferase (C=108.205.93; T1=106.206.67; T2=108.006.13
U/L); alanina aminotransferase (C=50.221.86; T1=51.891.70;
T2=52.101.49 U/L); total bilirubin (C=0.1000.005; T1=0.1100.002;
T2=0.1200.006* mg/dL); direct bilirubin (C=0.070.01; T1=0.060.01;
T2=0.100.01* mg/dL); indirect bilirubin (C=0.0400.007; T1=0.0500.006;
T2=0.0300.004 mg/dL); alkaline phosphatase (C=138.9012.72;
T1=130.705.16; T2=121.806.77 U/L); gamma-glutamyl transpeptidase
(C=6.000.37; T1=5.560.18; T2=6.000.21 U/L); total cholesterol
(C=99.5611.13; T1=83.005.91; T2=78.203.35 mg/dL); uric acid
(C=1.030.06; T1=0.960.06; T2=1.030.05 mg/dL). Conclusions: Oral
treatment with aqueous extract of stem bark of C. ferrea for 30 days
presented low toxicity in rats. However, the increase in serum amylase
levels may be investigated in details.


134

CODE 129
PRELIMINARY STUDIES FOR THE USE OF ARTIFICIAL NEURAL
NETWORKS (ANN) IN THE STUDY OF RHEOLOGIC PROPERTIES OF
TUBERCULOSTATICS AND SOLID EXCIPIENTS

Navarro, M. V. M.
1
; Moura, T. F. A. L.
1
; Nbrega, G. A. S.
2
; Lavor, E. P.
3
;
Soares, D. S.
3

1
Programa de Ps-graduao em Desenvolvimento e Inovao
Tecnolgica em Medicamentos, Universidade Federal do Rio Grande do
Norte (PPgDITM/UFRN).
2
Programa de Ps-graduao em Engenharia Eltrica e Computao,
Universidade Federal do Rio Grande do Norte (PPgEEC/UFRN).
3
Federal do Rio Grande do Norte (PPgDITM/UFRN).
Corresponding author: marconavarro@hotmail.com

Introduction: Artificial neural networks (ANN) are mathematical
constructions able to learn the relationships between data. The ability of
the interpretation and decision in complex systems make ANN as
promising tools to optimize pharmaceutical formulations. Studies for the
development of a solid formulation containing isoniazid, rifampsin,
pyrazinamide and ethambutol showed that these drugs have serious flow
problems. Objectives: This work aims at the development and use of
optimizing ANN properties for optimization of flow properties of a powder
mixture of tuberculostatic drugs. Materials and Methods:
Backpropagation ANNs with a single hidden layer and two hidden layers
were developed using Levenberg-Marquardt algorithm to adjust the
synaptic weights. Carr index (IC), Hausner factor (FH) and repose angle
() wee used s oupus. Results and Discussion: The fit between
experimental data and those provided by ANN were quite satisfactory just
after the first training cycles, with percentage error ranging from 2% (FH) to
5% (IC) and no anomalous behavior observed in the error decay curve.
Data fitting for was poor; error percentage was close to 45% with
abnormalities in the error decay curve denouncing suffering in learning
process. Subsequent adjust of the network resulted in an adjustment of
data better than those obtained for IC and FH, with percentage error close
to 0%. Conclusions: Low complexity networks with good predictive
capacity were obtained. The predictive capacity and the error evaluation
parameters indicate an appropriate learning curve for classification of
rheological parameters of powders.

Financial Support: CAPES/CNPq.


135

CODE 130
GASTROPROTECTIVE EFFECTS OF ETHANOLIC EXTRACT FROM
SPONDIA PURPUREA ON EXPERIMENTAL GASTRIC ULCER
MODELS IN RODENTS

Dantas, A.M.
1
*; Silva, K.M.A.
1
; Coutinho, A.C.
1
; Santos, V.L.
1
; Silva Jnior,
E.D.
2

1
Department of Pharmacy, State University of Paraba, Brazil.
2
Department of Pharmacology, Federal University of So Paulo, Brazil.
Corresponding author: alexandremedeiros6@yahoo.com.br

Introduction: The Spondia purpurea, Anacardiacea, is a Brazilian plant
commonly known as Siriguela. The leaf of this herb has pharmacological
properties against several diseases such as gastric ulcer. Objective:
Therefore, the aim of this study was to evaluate the effect of ethanolic
extract from S. purpurea (EESP) on animal models of human gastric ulcer.
Materials and Methods: Adult male Wistar rats and Swiss mice were
used. Gastric ulcer was induced in rats by Ethanol (99.6%) and in mice by
Piroxican 20mg.Kg-1. The animals were pretreated orally with drug-free
vehicle 10mL.Kg-1 (negative control), EESP at doses of 250, 500 and
750mg.Kg-1, Lanzoprazole 30mg.Kg-1 (for Ethanol-induced gastric ulcer in
rats) or Cimetidine 20mg.Kg-1 (for Piroxican-induced gastric ulcer in mice).
Results and Discussion: Both models of gastric ulcer showed a
significant inhibition of the lesions when compared to negative control,
expressed as inhibition percentage. Ethanol-induced gastric ulcer in rats
was inhibited at about 34.21% by Lanzoprazole. However, EESP was able
to inhibit the lesions in 47.04, 53.93 and 74.07%, at doses of 250, 500 and
750mg.Kg-1, respectively. On the other hand, Piroxican-induced gastric
ulcer in mice was diminished in 60.45% by pretreatment with Cimetidine. In
this model, EESP decrease the gastric lesion levels in 24.29, 20.9 and
60.45%, at doses of 250, 500 and 750mg.Kg-1, respectively. Conclusion:
From our data we suppose that ethanolic extract from S. purpurea has a
similar antiulcerogenic activity to Lanzoprazole and Cimetidine.
Accordingly, we concluded that plant species studied has a
gastroprotective effect.

Financial support from CNPq/UEPB.


136

CODE 131
IMPACT ON HEMATOLOGICAL PARAMETERS OF L
ARGININE SUPPLEMENTATION IN PATIENTS WITH SICKLE
CELL ANEMIA IN USE OF HYDROXYUREA

Elias, D.B.D.
1
; Barbosa, M.C.
1
; Bandeira, I.C.J.
1
; Santos, T.E.J.
1
; Dutra,
L.L.A.
1
; Pedrosa, A.M.
1
; Silva, H.F.
2
; Machado, R.P.G.
2
; Martins, A.M.C.
1
;
Gonalves, R.P.
1

1
Laboratory of Hematology, Department of Clinical and
Toxicological Analyses, Federal University of Cear (UFC).
2
Hematology and Hemotherapy Center of Cear (HEMOCE).
Corresponding author: izabelcristina.farmacia@yahoo.com.br

Introduction: The sickle cell anemia (SCA) is a chronic hemolytic
anemia, characterized by vascular damage, mainly attributed to lower
availability of nitric oxide (NO). Hydroxyurea (HU), used in the treatment
of AF, induces the synthesis of HbF and acts as NO donor. The
erythrocyte arginase released during hemolysis consumes L-
arginine, substrate for NO production, compromising the action of HU in
inducing HbF. Objective: We assessed the impact of the
association of HU with L-arginine supplementation in blood parameters of
patients with SCA treated at University Hospital Walter Cantdio.
Materials and Methods: A total of 13 patients with SCA in the use
of HU were analyzed, as follows: Group I, patients with SCA in the use
of HU (> 1g/day) (n = 6) and Group II, patients with SCA in the use
of HU (> 1g/day) with L-arginine supplementation (250mg/day) for 12
weeks, which were followed in four stages (0, 4, 8 and 12 weeks) (n = 7).
Results and Discussion:We analyzed the mean and standard deviation of
Hb, MCV, HbF, WBC,Reticulocytes and lactate dehydrogenase (LDH) of
patients in Group II (at 0, 4, 8 and 12 weeks of combined use of L-
arginine + HU) n = 7, and we observed a significant increase in
the reticulocyte count is a non-significant increase of Hb and HbF. There
were no differences in other parameters. Conclusion: The L-arginine
supplementation in patients receiving HU induced increase in
erythropoiesis, being related to the levels of HbF, suggesting
that HU increases HbF levels using nitric oxide pathway.


137

CODE 132
ALTERAES HEMATOLGICAS EM CRIANAS COM TETRALOGIA
DE FALLOT SUBMETIDAS CIRURGIA CARDACA COM
CIRCULAO EXTRACORPREA

Arruda, A.B.L.
1
; Souza, I.P.
1
; Gonalves, R.P.
1
; Mota, A.M.
1
; Esteves,
B.S.
1
; Lima, D.M.
1
; Bandeira, J.A.
1
; Barbosa, M.C.
1
, Pedrosa, A.M.
1
;
Bandeira, I.C.J.
1

1
Laboratory of Hematology, Department of Clinical and
Toxicological Analyses, Federal University of Cear (UFC).
Corresponding author: izabelcristina.farmacia@yahoo.com.br

Introduction: Tetralogy of Fallot (T4F) is most common form of cyanotic
congenital heart disease. During cardiac surgery is used cardiopulmonary
bypass (CPB) with heart-lung machine, which can produce haematological
changes. Objective: To characterize the main hematological changes
induced by cardiopulmonary bypass (CPB) in children with T4F who
underwent cardiac surgery at the Hospital de Messejana, in
Fortaleza. Materials and Methods: We studied 175 children in pre-and
post-operative (PO) immediate heart surgery and 1, 2 and 7 days after
surgery. Count of red blood cells were analyzed (He), hemoglobin (Hb),
hematocrit (Ht), prothrombin time (PT), activated partial thromboplastin
time(APTT) and platelet count. Results and Discussion: Of the
34 children with T4F, 65.0% were female, aged between 5 and 10
years and weighing between 10 and 20 kg. The data analysis showed a
decrease in He, Hb and Ht in post-operative as a result of surgical
correction of T4F, which caused a reduction of cyanosis and
polycythemia and improves the functional capacity of patients. Significant
changes were observed in TAP (sec), Activity (%), INR and platelets. As
for the APTT (sec), an increase in value after the surgery (POI) and, over
time, there was a decrease. Conclusion: The significant decrease
in platelet count after surgery demonstrates the ability to
differentiate groups of children with cyanotic more or less likely the risk
of thrombocytopenia and bleeding in the times studied.


138

CODE 133
PROFILE OF PATIENTS WITH CHRONIC DISEASE NON-
TRANSMISSIBLE PARTICIPANTS OF A PHARMACEUTICAL CARE
PROGRAM

Rodrigues, T. P.
1
; Cartaxo, N. A. O.
1
; Oliveira, A. R.
1
; Brando, D. O.
1
;
Silva, P. C. D.
1
; Silva, F. R.
1
; Queiroz, M. do S. R.
2

1
Discente do curso de Farmcia, Departamento de Farmcia,
2
Docente da disciplina de Assistncia Farmacutica, Departamento de
Farmcia, Universidade Estadual da Paraba (UEPB).
Corresponding author: thiagopeixotocg@gmail.com

Introduction: Hypertension and diabetes mellitus are public health
problems responsible for cardiovascular events that cause early disability,
death and for the public sector increased hospital costs. Objective:
Tracing the profile of people with chronic disease non- transmissible.
Materials and Methods: This study was conducted from March to June
2009, in the Municipal Health Service, Campina Grande-PB.It was
evaluated blood pressure and glucose levels, so the patients were oriented
about the disease appears, pharmacotherapy and they received
educational folders. All data collection was done through the records of the
Pharmaceutical Care Program.The data were evaluated using the Epi-info
3.5. Results and Discussion: Of the 62 participants, most of them were
female (64%) and 69% just had hypertension. In the evaluation of
metabolic disorders, 65% of the participants had metabolic syndrome, and
the blood pressure (BP), central obesity and HDL cholesterol were the
main components changed. As for the dyslipidemia types, it were more
prevalent hypercholesterolemia associated with hypertriglyceridemia and
low HDL. The most used pharmacological groups were diurects and
inhibitors of angiotensin I converting enzyme. Conclusions: The practice
of pharmaceutical care contributes to the control of blood pressure, bood
glucose and metabolic disorders, however its necessary to intensify the
actions encouraging the practice of healthy habits that correspond to
proper diet and physical activity.

Financial support from UEPB/Brazil.


139

CODE 134
PREPARATION AND CHARACTERIZATION OF INCLUSION
COMPLEXES BETWEEN CHLORPROPAMIDE AND -CYCLODEXTRIN

Dasmasceno, G. A. B.1; Freire, F. D.2; Raffin, F. N.1

1
Depto. de Farmcia CCS - Univ. Federal do Rio Grande do Norte -
UFRN, R. General Gustavo Cordeiro de Farias, S/N CEP: 59010 180 Natal
(RN) Brasil.
2
Ps-graduao em desenvolvimento e inovao tecnolgica em
medicamentos PGDITM UFRN. Campus Universitrio Av. Senador
Salgado Filho, 3000 Lagoa Nova CEP 59.072-970 Natal (RN) Brasil.
Corresponding author: gabriel_azevedo_14@hotmail.com

Introduction: Chlropropamide (CLP) is a sulfonylurea acting on pancreas
beta cells that are still in activity. On Biopharmaceutics Classification
System, CLP is placed in class II because of its high permeability and poor
solubility which can lead to decreased efficacy of oral treatment. Inclusion
complexes (IC) with cyclodextrins are an interesting strategy to increase
the solubility of hydrophobic molecules as CLP. Objective: The aim of this
work was to evue he effecs of CD on he ppen souby of CLP
by the phase solubility technique, comparing IC obtained by different
techniques. Materials and Methods: Phase solubility studies were
performed in accordance to Higushi and Connors, CLP-CD complexes
were prepared by physical mixture (MP), kneading (KD) and freeze-drying
(FD) and analyzed through differential scanning calorimetry (DSC).
Solubility was evaluated by the addition of an excess amount of each IC to
5mL of water and the concentration of CLP has been determined by
spechophoomey UV (=232nm). Results and Discussion: Phase
solubility diagram showed an AL complex (K=175.665M-1). MP, KD and
FD showed an increase on the CLP apparent solubility and DSC curves,
different levels of inecon beween CLP nd CD fo MP nd KD nd
the formation of a true CI for FD. Conclusions: The results showed that
ICs are an interesting alternative to the poor CLP solubility which can
improve effectiveness of dosage forms used in the treatment of Diabetes
mellitus.


140

CODE 135
TOXICOLOGICAL PROFILE OF EMERGENCY ATTENDED AT VIOSA-
AL HOSPITAL

FERREIRA, D. G.
1
; MIRANDA, N.
1
; GOMES, N. S.
1
; DE MOURA, M. A. B.
F.
1

1
Laboratrio de Biocincia Farmacuticas, Ncleo de Toxicologia. Escola
de Enfermagem e Farmcia UFAL.
Corresponding author: alinefidelis@gmail.com

Introduction: This research aimed to identify cases of toxicological
emergencies, reported in 2010 the municipality of Viosa-AL, for this it is a
representative of the central municipality of the state. Materials and
Methods: The survey was conducted in Viosa Municipal Hospital,
between January and March 2011 on the period from February to
December 2011 were examined medical records of emergency, after
evaluation of each record, if it were found some poisoning case,
information was collected as sex, age, date, diagnosis and therapy
employed, then this information was compiled. Results and Discussions:
At the end of the study were identified two hundred fifty-five poisoning
cases, among them cases of alcohol poisoning, food, medicinal, poisonous
animals typical of the region, and cases of poisoning by disinfectants,
pesticides, poisoning volunteers, without apparent cause poisoning, among
others. It was noted that monthly were around two thousand to two
thousand and five hundred patients. Typically, cases of poisoning were in
the hospital care, only in really severe cases, is that the patient was
referred to the capital. Conclusion: Through this survey it was concluded
that the cases are the higher incidence of food poisoning, alcohol
poisoning and scorpion bites, respectively. It was also observed that there
was no specific toxicological analysis to identify the possible toxicant
involved in poisoning. Was also suggested an underreporting of cases of
poisoning.

Financial support from FAPEAL.


141

CODE 136
DEVELOPMENT AND VALIDATION OF HPLC FAST METHOD FOR
SIMULTANEOUS DETERMINATION OF NINE POLYPHENOLS
COMPOUNDS IN CRUDE EXTRACTS OF PLANTS

Araujo-Silva, G.
1
; Solon, L.G.S.
2
; Santos, R. N. P.
1
; Morais, L. V. F.
1
;
Lpez, J. A. R.
1
; Almeida, M. G.
1

1
LABMULT, Department of Clinical and Toxicological Analyses -
UFRN/Natal-RN/Brazil.
2
Quality Control of Drugs Laboratory, Department of Pharmacy-
UFRN/Natal-RN/Brazil.

Corresponding author: gabriel_ar4@yahoo.com.br

Introduction: A development and validation assay methods is an
important part of the quality control of natural products, and such assays
are required by Brazilian health authorities for registration of
phytomedicines. Objective: The aim of this work was describe a fast
chromatographic method for determination of polyphenolic compounds in
crude extract of plants from Northeast of Brazil. Methods: A reversed-
phase mode in a C18 column (100 x 4.6 mm id, particle size 2.6 m) and
UV absorption spectroscopy. The elution gradient was generated from
0.1% formic acid aqueous solution and acetonitrile as an organic modifier.
Experimental conditions including pH, percentage of organic modifier and
elution gradient profile have been thoroughly optimized. Linearity ranges,
precisions, detection limits and stability have been established under
selected experimental conditions using synthetic standards and extract
solutions. Results and discussion: The use of an analytical column
packed with 2.6 mm particles has contributed to achieve an efficient
separation of analytes in less than 20 min. The use of the experimental
design and the multicriteria approach has facilitated the optimization of the
separation to reach the best compromise among resolution of close peaks
and analysis time in a limited number of assays. Conclusion: The method
has been applied to analyze crude extracts of plants from Northeast of
Brazil, according to Brazilian legislation (RE 899); this seems to be a
significant advantage with respect to other methods described in the
literature for similar analyses.

Finnantial supported from CNPq/Brazil.


142

CODE 137.1

APPLICATION OF DIFFERENTIAL THERMAL ANALYSIS (DTA) TO
DETERMINE OF TRIOXSALEN-EXCIPIENT COMPATIBILITY

Barros, D. M. C.
2
, Lima, N. G. P. B.
1,2
; Lima, I. P. B.
2
; Oliveira T. S.
2
;
Medeiros, A. C. D.
1
; Gomes, A. P. B.
2
; Arago, C. F. S.
2

1
Department of Pharmacy, Stadual University of Paraiba UEPB.
2
Laboratory of Quality Control , Department of Pharmacy, Federal
University of Rio Grande do Norte UFRN, Brazil.
Corresponding author: denise.michelle04@gmail.com

Introduction: Trioxsalen plus ultraviolet (UVA) and photochemotherapy
(PUVA) is frequently used in psoriasis and other dermatoses (Edward;
Jeffrey, Laskin, 1991). Objective: The purpose of the present work was to
study the compatibility of trioxsalen (TX) with different pharmaceutical
excipients using differential thermal analysis (DTA). The samples were
analyzed by TG/DTA. Materials and Methods: The curves were obtained
on a SHIMADZU thermobalance, using an alumina crucible, at heating
speed of 10C.min
-1
, on a temperature interval of 35 900C, under an
atmosphere of nitrogen at 50 mL.min
-1
. Sample weight was around 8 mg.
The mixtures in a 1:1 (w/w) were prepared by mixing the drug:excipient. The
excipients used were magnesium stearate (MS), colloidal silicon dioxide
(CSD - aerosil), lactose monohydrate (o-lactose), microcrystalline cellulose
PH 102 (MC 102), pregelatinized starch (starch), mannitol, sodium lauryl
sulfate (SLS), sodium starch glycolate (SSG) and croscarmellose sodium
(CCS). Results and Discussions: The DTA curve of trioxsalen present a
sharp endothermic event at 234
o
C indicating the melting and which
corresponds to the values indicated in literature (234-235
o
C). The adjuvant
that showed interactions was SLS used in a 1:1 ratio. These results
indicated the occurrence of a strong interaction in the solid state with
temperature, but not necessarily corresponding to incompatibility.
Conclusion: The compatibility of trioxsalen with various pharmaceutical
excipients could be tracked by DTA measurements. The SLS presented
thermal interactions with the TX.

Financial Support from PROPESQ-REUNI-UFRN, UEPB, CNPq, CAPES
and FAPERN.


143

CODE 137.2
COMPATIBILITY STUDY OF VITAMIN D3 AND MANNITOL IN
PHYSICAL MIXTURES

Barros, D. M. C.
2
; Lima, N. G. P. B.
1,2
; Lima, I. P. B.
2
; Fernandes, F. H. A.
1
;
Correia, L. P.
1
1
; Arago, C. F. S.
2

1
Department of Pharmacy, Stadual University of Paraiba UEPB.
2
Laboratory of Quality Control, Department of Pharmacy, Federal
University of Rio Grande do Norte UFRN, Brazil.
Corresponding author: denise.michelle04@gmail.com

Introduction: Vitamin D, the sunshine vitamin, is now recognized not only
for its importance in promoting bone health in children and adults but also
for other health benefits, including reducing the risk of chronic diseases
such as autoimmune diseases, common cancer, and cardiovascular
disease (Edward; Jeffrey, Laskin, 1991). Objective: The purpose of the
present work was to study the compatibility of Vitamin D3 with mannitol
using differential scanning calorimetry (DSC). Materials and Methods: The
DSC curves were obtained using a DSC Q20 (TA instrument) under
dynamic nitrogen atmosphere with the flow rate of 50 mLmin
-1
. 2 mg of
samples were weighted out and placed in a sealed aluminum pan. The
analysis was carried out from 25 - 450 C at a heating rate of 10C min-1.
The mixtures in a 1:1 (w/w) were prepared by mixing the drug:excipient.
Results and Discussions: The DSC curve of vitamin D3 present a sharp
endohemc even 85 C ndcng he meng nd whch coesponds o
the values indicated in literature (84-85 C). A sharp melting endotherm was
observed at 167 C in the DSC of mannitol. DSC curves of vitamin D3-
mannitol mixture showed an endothermic peak of drug at 85 C, indicating
that there was no interaction. DSC data showed compatibility of vitamin D3
with diluent mannitol. Conclusion: However, further studies should be
performed such as FTIR and SEM to confirm the findings.

Financial Support from PROPESQ-REUNI-UFRN, UEPB, CNPq, CAPES
and FAPERN

.


144

CODE 138
A QUANTUM STUDY OF DRUG-RECEPTOR INTERACTION:
RECONHECENDO AS BINDING ENERGIES BETWEEN 16S rRNA
RESIDUES AND HYGROMYCIN B

Oliveira, J. I. N.
1
; Fulco, U. L.
1
; Albuquerque, E. L.
1
; Freire, V. N.
2
; Neto, J.
X. L.
1

1
Laboratory of Biophysics and Computational Simulation, Department of
Biophysics and Pharmacology, at Federal University of Rio Grande do
Norte, 59072-970 Natal, RN, Brazil.
2
Department of Physics at Federal University of Fortaleza, 60455-760,
Fortaleza, CE, Brazil.
Corresponding author: jonasivan@gmail.com

Introduction: The Hygromycin B (HygB) is an aminoglycoside initially
isolated from Streptomyces hygroscopicus, and as such inhibits the
bacterial protein synthesis through irreversible binding to the 16S
ribosomal RNA (16S rRNA) of the bacterial 30S ribosomal subunit (30S).
Objective: Due to strong electrostatic character of this connection, we
propose an energetic investigation of binding mechanism of this complex
using dielectric constants, which have been widely used to study the
electrostatic properties of biomolecules. Materials and Methods: For this,
we calculated the drug-binding energy of each nucleotide inside the
Hygromycin B recognition site on 30S bacteriam ribossome by using the
molecular fractionation with conjugate caps (MFCC) strategy. Results and
Discussion: We saw that high E values underestimate the individual
interaction energy between HygB and each 16SrRNA nucleotide of binding
site, and quickly enable the convergence state of the total binding energy.
From an efficient process of convergence, we define a suitable binding
pocket encompassing all major residues able to stabilize the Hyg B in the
30S ribosome. Thus, identify that C1403, C1404, G1405, A1493, G1494,
U1495, C1496 and U1498 are the residues that interact more strongly with
the drug. These residues probavly lead to a state of drug resistance in
bacteria when they suffer single base substitutuions, whereas those with
low interaction energy probably will not effectively influence the affinity of
the binding pocket Hygromycin B and therefore would not generate
resistance when mutated.

CAPES/Brazil).


145

CODE 140
STUDY OF 599 C>T POLYMORPHISM OF THE GPX1 GENE IN TYPE 1
DIABETES: IMPLICATIONS IN ANTIOXIDANT DEFENSE AND
DIABETIC NEPHROPATHY

Oliveira, Y. M. C.
1
; Souza, K. S. C.
1
; Silva, H.P.V.
1
; Ururahy, M. A. G.
1
;
Loureiro, M. B.
1
; Oliveira, G. H. M.
1
; Morais, L. V. F.
1
; Arrais, R. F.
2
;
Abdalla,

D. S. P.
3
; Hirata, R. D. C.
3
; Hirata, M. H.
3
; Rezende, A. A.
1
;
Almeida, M. G.
1

1
Department of Clinical and Toxicology Analyses - UFRN/Natal-RN/Brazil.
2
Department of Pediatrics- UFRN/Natal-RN/Brazil.
3
Department of Clinical and Toxicology Analyses- USP/So Paulo-SP-
Brazil.
Corresponding author: mgalmeida@digi.com.br

Introduction: Relationship between reactive oxygen species (ROS) and
diabetes is well established in literature. Genetic variations in antioxidants
enzymes such glutathione peroxidase (GPX) can result in reduction of their
antioxidant potential and predispose to development of diabetic
nephropathy (DN). Objective: Evaluate the influence of GPX1 599 C>T
polymorphism in erythrocyte activity of GPX and GSH content and their
implications in diabetic nephropathy in DM1 patients assisted at a pediatric
hospital (HOSPED/UFRN) in Natal-RN/Brazil. Methods: 77 DM1 patients
and 91 normoglycemic subjects (NG) between 6 and 20 years were
recruited. GPX1 (599 C>T) polymorphism was determined by allelic
discrimination technique in real-time PCR. GSH content was quantified as
described by Beutler. Erythrocity GPX activity was determined using
commercial kits by Randox. Albumin/creatinine (RAC) ratio was
determined using kits by Biosystems. Results and discussion: The
content of GSH was significantly lower in DM1 patients comparing NG
subjects (p=0,024). The red cell GPX activity was significantly higher in
DM1 group (p= 0,001). Eleven DM1 patients showed RAC> 30 g/mg
characterizing microalbuminuria recognized as early sign for detection of
DN. The presence of the 599T allele was associated with a decrease GSH
content (p= 0,002) and lower erythrocyte GPX activity (p=0,001) in DM1
patients, leaving them more vulnerable to oxidative damage. Conclusion:
These findings suggest a deleterious role of the 599T allele (GPX1) in
antioxidant defense of patients with DM1 leading to accumulation of ROS
which could contribute to the onset of diabetic nephropathy evidenced by
elevated RAC in patients studieds.

Financial supported from CNPq/Brazil.


146

CODE 141.1
PRELIMINARY STUDY FOR THERMAL CHARACTERIZATION OF
BIOIDENTICAL HORMONE ESTRADIOL

Pereira, T. M. M.
2
; Silva, M. M. C.
1
; Penha, S. L. T.
1
; Gomes, A. P. B.
3
;
Arago, C. F. S.
3

1
Undergraduate, Quality Control of Drugs Laboratory (LCQMed),
Pharmacy Department (DFAR), Federal University of Rio Grande do Norte
(UFRN).
2
Posgraduate LCQMed, DFAR, UFRN.
3
Professor, LCQMed, DFAR, UFRN, Brazil.
Corresponding author: thereza_mylene@yahoo.com.br

Introduction: Bioidentical hormones are compounds that have exactly the
same

chemical and molecular structure as endogenous human hormones.
Health care providers often prescribe hormones for menopausal women
symptoms. Studies about thermal behavior of Bioidentical Hormones using
thermal techniques are scarce in the literature. Objective: This study aims
to thermal characterization, through curves TG / DTA, to get the best event
for the quantification Estradiol. Materials and Methods: Bioidentical
Hormone Estradiol was analyzed by TG/DTA. The curves were obtained
on a SHIMADZU thermobalance, model TG/DTA, using an alumina
crucible, at heating speed of 20C/ min
1
, on a temperature interval of 25
900C, under an atmosphere of nitrogen at 50 mL/ min
1
. Sample weight
were around 0.1; 0.2; 0.5; 1; 2; 4; 6; 8; and 10 mg. Results and
Discussion: DTA curves of Bioidentical Hormone Estradiol, present four
events and TG curve only one thermo decomposition processes. Thermal
data of the Bioidentical Hormone Estradiol supply important information on
evaluation of stability of the materials, becoming an important tool on
quality control and pharmaceutical technology of on the development of
products. The Thermal event B showed the best Linearity correlation (R= -
0,99984) in DTA method between four events. Conclusions: TG/DTA
study of Bioidentical Hormone Estradiol show your thermal stability, and this
parameters can be used in the development and characterization of
pharmaceutical products.

Financial Support from PROPESQ-REUNI-UFRN, CNPq, CAPES and
FAPERN.


147

CODE 141.2
LINEARITY IN DTA METHOD: A COMPARISON OF TWO
QUANTIFICATION STRATEGIES OF BIOIDENTICAL HORMONE

Pereira, T. M. M.
2
; Silva, M. M. C.
1
; Penha, S. L. T.
1
; Gomes, A. P. B.
3
;
Arago, C. F. S.
3

1
Undergraduate, Quality Control of Drugs Laboratory (LCQMed),
Pharmacy Department (DFAR), Federal University of Rio Grande do Norte
(UFRN).
2
Posgraduate LCQMed, DFAR, UFRN.
3
Corresponding author: thereza_mylene@yahoo.com.br

Introduction: Bioidentical Hormone Replacement Therapy describes
supplementation of hormones that are molecularly identical to those
hormones produced in the human body such as Estriol Bioidentical. TG
DTA/DSC is the quick and convenient techniques and has been widely
used to characterize the changes of mass and enthalpy of materials during
the thermal decomposition. Objective: Conduct the study of thermal
characterization through DTA curves in order to get the best event for
quantification of Bioidentical Estriol. Materials and Methods: It was used
Estriol drug. Non-isothermal thermogravimetric curves of Estriol drug were
obtained using a Shimadzu model DTG-60 thermobalance at 20C.min
1

heating rates up to 900C in a nitrogen (flow rate: 50 mL.min
1
). The curves
were obtained on several of initial sample mass (0.1, 0.2, 0.5, 1.0, 2.0, 4.0,
6.0, 8.0 and 10.0 mg) in alumina crucible. Results and Discussion: In
DTA curves was observed two events, fusion (from 280 to 315C) and
degradation (from 350 to 430C). The Thermal event A (fusion) showed the
best Linearity correlation (R= -0,99965) in DTA method that the event B
(degradation). Conclusions: The linearity in DTA method showed
endothermic event A could be used between 0.142 and 10.041 mg to
quantification of pharmaceutics products with Bioidentical Hormone Estriol.

Financial Support from PROPESQ-REUNI-UFRN, CNPq, CAPES and
FAPERN


148

CODE 142
INTERFERENCE ON ADHERENCE OF Candida ssp IN CONTACT
LENSES BY MICROEMULSION SYSTEMS

Silveira, W. L. L.
1
; Silva, K. S.
1
; Ferreira, L. F.
1
; Farias, M. G. I.
2
; Shinobu,
C. S.
2
; Svidzinski, T. I. E.
2
; Egito, E. S. T.
1*

1
2
Laboratrio de Micologia, Universidade Estadual de Maring (UEM).

Introduction: Biofilms represents the most prevalent type of microbial
growth in nature. Investigations of fungal biofilms associated with contact
lenses are virtually nonexistent and the lack of robust in vitro models has
resulted in scant information about the pathogenesis of Candida keratitis.
Objectives: The aim of this work was to study the interference on the
adherence, first step for biofilm shaping by Candida strains, in contact
lenses, submitted to the exposition of a microemulsion system (ME).
Materials and Methods: Isolated and ATCC yeast of Candida albicans
and Candida tropicalis were incubated at 25C 2.5 over 24 hours. Fungal
cells were suspended, after the growth, and prepared at the concentration
of 10
2
cfu in saline solution (0.85%) or ME samples to guarantee their
viability. Four hours later the cells were inserted in 24 well plates
containing the contact lenses. To evaluate the fungal adherence capacity,
the Shins method was adapted and used. Results and Discussion:
Compared to the control, the ME completely impeached the adherence of
the strains. The interference induced by the ME on the biofilms formation
was probably due to the surfactants on the formulation, which disturbers
the cell membrane causing changes in the biofilms shaping. Conclusions:
The biofilm formation by Candida spp strains were inhibited by the
influence of the ME. The surfactants of the ME composition may be the
main reason of this interference because of they are able to compromise
the stability of the lipids contained in the membrane cells, turning them
unstable.

Financial Support from CNPq/Brazil and Procad NF 2008/ Capes.


149

CODE 143.1
CHARACTERIZATION OF THERMAL EVENTS OF HYDROQUINONE
DEPIGMENTANT BY DTA

Oliveira, T.S.
1
; Barros, D.M.C.
1
; Lima, I.P.B.
2
; Lima, N.G.P.B.
2
; FERRARI,
M.
3
; Arago, C.F.S.
3
*

1
Undergraduate,
2
Postgraduate and
3
Professor, Federal University of Rio
Grande do Norte UFRN, Brazil.
Corresponding author: ciceroaragao@ufrnet.br

Introduction: The depigmentant drug mostly used in the treatment of
melasma is hydroquinone (HQ)
[1]
. Materials and Methods: Differential
thermal analysis (DTA) has been used for characterization of the materials
[2]
.
The aim of this work was to characterize the thermal events of hydroquinone
by DTA. The HQ was analyzed by TG/DTA, on a SHIMADZU
thermobalance, in the 25-900C temperature range, under an atmosphere of
nitrogen, at heating speed of 10C min
-1
with different sample weights of 3, 5
and 7 mg. The DTA curves were used to identify thermal events as well as
the temperatures and energies involved in these events. Results and
Discussions: DTA curves present two thermal decomposition events and
shows that for the sample weight of 3mg the first event happens at T
onset
of
172C, T
peak
175C, T
endset
182C, 1180 J/g, 282 Cal/g and the second at
T
onset
192C, T
peak
205C, T
endset
212C, 1307 J/g, 312 Cal/g. For the weight
of 5mg the first event at T
onset
of 172C, T
peak
175C, T
endset
183C, 1162 J/g,
277 Cal/g and the second at T
onset
195C, T
peak
212C, T
endset
221C, 1664
J/g, 397 Cal/g. For the weight of 7mg the first event at T
onset
of 173C, T
peak
176C, T
endset
185C, 1270 J/g, 303 Cal/g and the second at T
onset
196C,
T
peak
219C, T
endset
229C, 1984 J/g, 473 Cal/g. The results show good
correlation between the thermal events and the change in sample weights.
Conclusion: DTA can be useful in the characterization of thermal events
of pharmaceutical materials.

FAPERN, CNPq and CAPES by financial support.

.


150

CODE 143.2
COMPATIBILITY STUDY OF TRETINOIN AND EMULSIFYING AGENT
IN PHYSICAL MIXTURES

Oliveira, T. S.; Barros, D. M. C.; Lima, I. P. B.; Lima, N. G. P. B.; Ferrari,
M.; Arago, C. F. S.

Laboratory of Quality Control, Department of Pharmacy, Federal University
of Rio Grande do Norte UFRN, Brazil.
Corresponding author: thayssan10@hotmail.com

Introduction: Tretinoin has been used in the management of skin disorder
to improve the appearance and texture of skin. Topically, tretinoin has
been used for treatment of ichtyosis, psoriasis, acne vulgaris, neoplasia
and other skin disease at various dosage forms, such as solutions, lotions
ointments and creams. (Shin et al., 2005). Objective: The aim of the
present work was to perform the compatibility study between tretinoin and
cetostearyl alcohol using differential thermal analysis (DTA). Materials and
Methods: The samples were analyzed by TG/DTA. The curves were
obtained on a SHIMADZU thermobalance, using an alumina crucible, at
heating speed of 10C.min
-1
, on a temperature interval of 35 900C, under
an atmosphere of nitrogen at 50 mL.min
-1
. The mixtures in a 1:1 (w/w) were
prepared by mixing the drug:excipient. Results and Discussions: The DTA
curve of tretinoin present a sharp endothermic event at 184
o
C indicating the
melting. A sharp melting endotherm was observed at 54
o
C in the DTA of
cetostearyl alcohol. The result of tretinoin in the presence of cetostearyl
alcohol was shifting in tretinoin-cetostearyl alcohol mixture, which
suggested incompatibility. DTA data showed incompatibility of tretinoin with
emulsifying agent. Conclusion: In this case, further studies will be
performed with other tecnhiques, in order to gain insight into the nature of
the interaction and to estabilsh an actual pharmaceutical.

Financial support from PROPESQ-REUNI-UFRN, CNPq, CAPES and
FAPERN


151

CODE 144
EVALUATION OF THE ANTINOCICEPTIVE EFFECT OF
HYDROETANOLIC EXTRACT OF Portulaca pilosa L
(PORTULACACEAE) in mice

Alexopulos, F. F.
1
; Melo, A. S.
1
; Dolabela, M. F.
1
; Sousa, P. J. C.
1

1
Laboratory of Pharmacodynamics, Faculty of Pharmacy/ Post-Graduate
Program in Pharmaceutical Sciences, Federal University of Par, UFPA.

Introduction: The specie Portulaca pilosa belong the family Portulacaceae
and genus Portulaca. Has wide distribution in northern Brazil and is
popuy ced mo-cescdo. Objective: To evaluate the
antinociceptive effects of the of hydroetanolic extract of Portulaca pilosa L.(
HEPp) in mice. Materials and Methods: In the writhing test the animals
were pre-treated orally 1 h before to the acetic acid 0,6% with doses of 50,
100, 200 and 400 mg / kg, vehicle and indometacin (5 mg/Kg).The
numbers of contortions was recorded for a period of 20 min. The Hot plate
test was used to evaluate the effect of the extract (200 e 400 mg/Kg) and
morphine (10 mg/Kg) on nociception centrally mediated.The formalin test
was assayed to study the effects of the extract (100, 200 and 400 mg/Kg)
on the nociception of neurogenic and inflammatory origin. To investigate
the opiod receptors involvement was administered the opioid antagonist
naloxone before the extract and morphine. Results: The doses of 100, 200
and 400 mg/kg inhibited significantly the abdominals contortions when
compared to control group. In hot plate test, the extract no change the
latency when compared to control, unlike morphine. In formalin test, the
doses of 200 e 400 mg/kg orally significantly inhibited the 1st and 2nd
phase of the assay. In addition, the opioid antagonist naloxone reversed
the effect of the HEPp in 1st and 2nd phases. Conclusions: The results
show that the HEPp orally exerts antinociceptive effect on nociception of
neurogenic and inflammatory origin and opioids receptors are involved in
this effects.

Finnacial Support from FAPESPA/Brazil and master scholarship from
CAPES /Brazil.


152

CODE 145
EVALUATION OF PHYSICOCHEMICAL PROPERTIES OF POORLY
WATER SOLUBLE DRUG (SIMVASTATIN) OBTAINED BY
RECRYSTALLIZATION

Vargas, M. R. W.
1
, Lima Neto, S. A.
2
, Torres, S. M.
3
, Moura, T. F. A. L.
1

1
Laboratory of Medicine Development Health Sciences Center /UFRN,
Natal, Brazil.
2
Unified Laboratory of Development and Testing Medicines - Health
Sciences Center/ UFPB, Joo Pessoa, Brazil.
3
Department of Mechanical Technology, Technology Center/UFPB, Joo
Pessoa, Brazil.
Corresponding author: mararubia38@hotmail.com

Introduction: The recrystallization of drugs is one of several strategies to
increase the solubility of Drug Class II (Biopharmaceutics Classification
System). Simvastatin is one this type of drug of such class that have low
water solubility as well as high intestinal permeability. However, the
formation of one or more simvastatins polymorph during the process of its
production can consequently change both physical and chemical
properties. Objective: To evaluate the physical and chemical properties of
simvastatin obtained by recrystallization with organic solvents. Materials
and Methods: Simvastatin was solubilized in different solvents
(dichloromethane, ethyl acetate, acetone, methanol). The samples were
dried in a rotary evaporator under reduced pressure, placed in a desiccator
and the new crystalline forms were evaluated by Infrared Spectroscopy
and X-ray powder diffraction (XRD). The results were compared with
simvastatin raw material (negative control). Results and Discussion: The
infrared spectra of the samples showed bands similar to the controls,
suggesting that there was not physical-chemical alteration of simvastatin
during the recrystallization. The XRD patterns of the same samples
presented similar peaks then the raw material, demonstrating that there
was no change in crystallinity and, consequently, no formation of
polymorphs. Conclusions: After recrystallization of the drug, the formation
of amorphous material was not observed. This observation might lead to
an improved solubility. Also, there was no change in the physicochemical
characteristics of the drug which gives evidence to its stability.

Financial support from CAPES/Brazil.


153

CODE 146
ANALYSIS AND CHARACTERIZATION OF OILS AND FATS

Silva, E. I. L.
1
; Valena, R. C. A.
1
; Gurgel, R. K. C.
1
; Oliveira, R. A.
1
;
Pagnoncelli, M. G. B.
1
; Assis, C. F.
1

1
Laboratory Bromatology, Department of Pharmacy, Universidade Federal
do Rio Grande do Norte.
Corresponding author: erlhane_ls@hotmail.com

Introduction: Lipids are macromolecules of biological importance that can
be synthesized in the organism except the essential fatty acids. They can
be found in solid form (fat) or liquid (oil). Transformations that can occur in
lipids affect its organoleptic qualities, it may cause toxic effects due to
intake of these foods. Objective: Check the quality of samples of oils
marketed by checking if they are within the standards of identity and quality
of Brazilian legislation. Methods: In the analysis we used five samples of
soybean oil called AO1, AO2, AO3, AO4 and AO5, these were subjected to
four tests: index of saponification, index iodine, index acid and index
peroxide. Results and Discussion: The tests were performed for five
samples and the values above the reference results were: 40% for the
index of saponification, 20% for iodine, 20% for the index of free acidity
and 20% for the peroxide. Two tests showed results below the reference
values: 20% for iodine and 20% for the rate of saponification. The results
show that the changes can be found from the improper storage causing
changes in reference values. Moreover, the difference in average weight of
fatty acids can occur due to tampering with other types of oil.
Conclusions: The samples showed mostly favorable results being
adequate for consumption. However some samples need further
investigation, due to the unsatisfactory results obtained, for this new
analysis can be performed with oils from the same batch for a proof.


154

CODE 148
EVALUATION OF ANTIOXIDANT ACTIVITY IN SEASONAL STUDY OF
PHASE ETHYL ACETATE Bauhinia cheilantha (Bong.) Steudel

Oliveira, A. M. F.
1
; Nbrega, F. F. F.
2
; Pereira, C. K. S.
1
; Penha, A. R. S.
1
;
Almeida, R. N.
1,2
; Assis, T.S.
3

1
Programa de Ps-Graduao em Produtos Naturais e Sintticos
Bioativos, Universidade Federal da Paraba (UFPB).
2
Programa de Ps-Graduao em Biotecnologia Rede Nordeste de
Biotecnologia (RENORBIO).
3
Departamento de Fisiologia e Patologia, Universidade Federal da Paraba
(UFPB).
Corresponding author: franklinnobrega@yahoo.com.br

Introduction: Reactive oxygen species (ROS), derived from the
metabolism of oxygen, molecules react with circulating molecules, being
essential in many biological systems. Excess ROS may contribute to
various diseases such as diabetes, cancer. Natural antioxidants can
increase the body's natural resistance, are important as defense
mechanisms of cells. The seasonal variation interferes with the production
of antioxidants substance. The leaves, stems and roots of Bauhinia
species are used for the treatment of diseases such as diabetes.
Objective: To determine the total phenolic content and evaluate the
antioxidant activity of Bauhinia cheilantha (Bong.) Steudel, collected in four
different seasons (col1, col2, col3, col4). Materials and Methods: The
total phenolic content was determined using the Folin Ciocalteau. The
antioxidant activity was determined using the DPPH radical, according to
the method of Silva et al. (2006). Results and discussion: The col4 had
the highest content of phenolic compounds (78.22 0.847 mg / EAG g), as
well as the best antioxidant activity (3.253 0.231 mg / mL) than the other
collections of the plant, with a good correlation between phenolic content
and antioxidant activity. Conclusion: The total phenolic content may be
responsible for the antioxidant activity of this species, moreover, that the
seasonal variation interferes with the production of secondary metabolites
such as phenolic substances. The antioxidant activity of plants depends on
growing conditions, soil conditions and water.



155

CODE 149.1
PHARMACEUTICAL ASSISTANCE THE ELDERLY: SHARES OF
COMMUNITY INTERVENTION IN BRASILIA TEIMOSA

Valena, R. C. A.
1
; Loureno, E. I. L.
1
; Gurgel, R. K. C.
1
; Oliveira, R. A.
1
;

Santos, K. C. L.
1
; Oliveira, A. R. A.
2
; Rezende, A. A.
2

1
Graduate Student of Pharmacy, Universidade Federal do Rio Grande do
Norte (UFRN).
2
Laboratory of Biochemistry Clinical, Department of Clinical and
Toxicological, Universidade Federal do Rio Grande do Norte (UFRN).
Corresponding author: renatavfarmacia@hotmail.com

Introduction: The pharmaceutical assistance aims to help ensure that
patients derive maximum benefit in the use of their drugs. Objective:
Develop pharmaceutical assistance activities in the community of Brasilia
Teimosa, municipality of Natal, in order to improve the response to drug
treatment and quality of life of elderly hypertensives and diabetics.
Materials and Methods: We analyzed 24 elderly for a period of six
months. They underwent a questionnaire relating to patient health, the
weekly visits to check blood pressure and monitoring of medication, as well
as lectures on generic drugs and tools to improve adherence to treatment.
Results and Discussion: With the analysis of questionnaires, it was
determined that 24 of the patients, 50% (12/24) have diabetes and
hypertension, 45.83% (11/24) hypertension and 4.17% (1 / 24) diabetes.
After the intervention was a significant improvement in blood pressure of
33.3% (8 / 24) elderly. One of the tools used to improve adherence to
treatment was the distribution of kits for the correct storage of medication,
45.83% (11/24) of patients received 41.66% (10/24) already had and 12.5
% (3 / 24) will receive it. Through weekly visits it was found that 75% used
medication correctly and 21% joined after the intervention, moreover, about
33.3% (8 / 24) came to use generic drugs after the intervention.
Conclusions: The patients treated had a significant increase from the
therapeutic response, as detected by checking blood pressure. It is
observed that the improvement in quality of life of elderly may be related to
pharmaceutical assistance.


156

CODE 149.2
PROTEIN ANALYSIS BY BRADFORD METHOD IN SAMPLES OF
CEREAL

Valena, R. C. A.
1
; Gurgel, R. K. C.
1
; Silva, E. I. L.
1
; Oliveira, R. A.
1
;
Pagnoncelli, M. G. B.
1
; Assis, C. F.
1

1
Laboratory Bromatology, Department of Pharmacy, Universidade Federal
Corresponding author: renatavfarmacia@hotmail.com

Introduction: Proteins are essential for the proper functioning of the
organism, exercising the most varied functions. There are many methods
for the determination of proteins, among them there is the method of
Bradford, based on the interaction of the dye BG-250 with high molecular
weight proteins with basic or aromatic side chains. Objective: To
determine the total protein concentration in samples of cereals.
Methodology: A total of five samples, two of wheat bran (A1 and A2), an
oat (A3) and two of corn flour (A4 and A5). In the first part of the method
was performed chemical extraction of proteins. In the second step the
samples were diluted in four different concentrations. In third was added
the Bradford reagent, with the subsequent reading of samples in the UV
visible spectrophotometer at a wavelength of 595 nm. To find the
concentration of protein was used a standard curve of albumin. Results
and Discussion: For samples A1 and A2 was obtained a value of 0,6 g of
proteins for 100 g of wheat bran. The sample A3, presented 0,3g
proteins/100g of oats. Sample A4 0,4 g proteins/100g presented corn flour.
Already the sample A5 showed a result of 0,2g proteins/100g of the
sample. Conclusion: In the observed data, it is verified that the cereals
are not direct sources of proteins, but when coupled with a balanced diet
can contribute to the daily intake of this nutrient, mainly wheat bran with the
highest protein concentration.


157

CODE 150
ANTIMICROBIAL ACTIVITY OF HYDROALCOHOLIC EXTRACT OF
Stryphnodendron adstringens Mart. AGAINST STRAIN OF
Staphylococcus aureus

Arajo, G. M. F.
1
; Almeida, C. L. F.
1
; Arajo, J. S. C.
1
; Ramos, K. R. L. P.
1
;
Eleamen, G. R. A.
1
; Cato, R. M. R.
2

1
Paraiba (UEPB).
2
University of Paraiba (UEPB).
Corresponding author: gabifarmacia@gmail.com

Introduao: Stryphnodendron adstringens Mart. Leguminosae-
Mimosaceae, conhecido como barbatimo so amplamente utilizadas na
medicina popular para o tratamento de diarrias, problemas ginecolgicos
e como a cicatrizao de feridas. Estudos anteriores mostraram
significativas propriedades cicatrizante, atividades anti-inflamatrias e
antimicrobianas. Objetivo: Os objetivos deste esudo fom v n
vo vdde nmcobn e Conceno Inb Mnm (MIC) do
extrato hidroalcolico de S. adstringens Mart. frente a cepas padres.
Materiais e Mtodos: Os microorganismos testados foram Escherichia
coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Pseudomonas
aeruginosa (ATCC 27853). O estudo foi realizado pelo mtodo de difuso
em disco (CLSI, 2005) dcondo 20L do extrato em discos de papel
filtro estril (6mm de dimetro), previamente identificados. As placas foram
incubadas a 37 C por 24h e posteriormente a leitura foi realizada. Foi
considerado ativo os halos de inibio do crescimento com dimetro
superior a 8 mm. Resultados e Discusso: a atividade antibacteriana do
barbatimo foi observada frente a S. aureus, e para as demais cepas no
foi observado a atividade. Para a cepa de S. aureus, o barbatimo foi ativo
at a concentrao de 12,5%. Concluses: O barbatimo apresentou-se
ativo nas condies analisadas apenas frente a cepa de S. aureus.

Financial support from CAPES / Brasil.


158

CODE 151
STUDIES OF MAGNETIC PARTICLES PREPARED BY CO-
PRECIPITATION UNDER MAGNETIC FIELD FOR DRUG DELIVERY

Silva, K. L.
1
; Freitas, E. L. S.
1
; Pontes, T. R. F.
1
; Araujo Neto, R. P.
1
;
Carvalho, J. F.
1
; Egito, E. S. T.
1
; Torres, M. M.
2
; Carrio, A. S.
3

1
Laboratrio de Sistemas Dispersos (LASID), Departamento de Farmcia,
2
Departamento de Cincias exatas e Naturais, Universidade Federal Rural
do Semirido.
3
Departamento de Fsica Terica e Experimental, Universidade Federal do
Rio Grande do Norte (UFRN).
Corresponding author: ascarrico@gmail.com

Introduction: Magnetic particles have been increasingly used in
biotechnology. Most applications, such as hyperthermia and magnetic drug
targeting, benefit from large values of the initial susceptibility and saturation
magnetization. Growing magnetite micro-particles by co-precipitation
involves the formation of small nano-crystallites and their aggregation. It is
thus possible to interfere in the magnetic structure of the micro-particles.
Objective: Tailoring the nano-structure of magnetite micro-particles,
particularly the relative orientation and size of nano-crystals, to obtain
particles with improved properties for drug targeting. Materials and
Methods: Magnetite particles were prepared by chemical co-precipitation
method of ferrous chloride and ferrous sulphate (2:1) with NaOH in both
the presence and absence of external magnetic field (MF), generated from
a pair of Nd-magnets. Moreover, the samples were analyzed by Mossbauer
Spectroscopy (MS) and Vibrating Sample Magnetometry (VSM). Results
and Discussion: MS and VSM revealed that the samples are
superparamagnetic. Also, the synthesis in the presence of an external MF
of 172mT favored an increase in the initial susceptibility of around 25%.
The saturation magnetization showed a larger increase (44%), from
40.27emu/g, for the control sample, to 59emu/g for the one grown in the
presence of a 172mT MF. Conclusions: It is possible to tailor the initial
susceptibility and the saturation magnetization of magnetite micro-particles
grown by co-precipitation by using an external MF during the synthesis. The
simple and easy MF assisted co-precipitation method may turn out to be
useful for preparing magnetic micro-particles for a wide range of biomedical
applications.

Financial support from CNPq/Brazil and CAPES/Brazil.


159

CODE 152
A STRUCTURAL AND ENERGETIC INVESTIGATION OF
PAROMOMYCIN-16S rRNA COMPLEX

Azevedo, J. L. C.
1
; Fulco, U. L.
1
; Albuquerque, E. L.
1
; Oliveira, J. I. N.
1
;
Costa, A. H. L.
1

1
Norte, 59072-970 Natal, RN, Brazil.
Corresponding author: jeh.lay@gmail.com

Introduction: The Paromomycin is an aminoglycoside antibiotic, which
binds to the 30S ribosomal subunit and promotes the inhibition of protein
synthesis by reducing the dissociation rate of tRNA site. From the binding
energy of the drug in the ribosome, it is possible to see which nucleotides
have the biggest interaction and which of them influence the effectiveness
of this connection. Objective: In this work, we implement a full quantum
mechanical calculation in the three-dimensional structure of 30S ribossome
subunit from T. thermophilus in contact with Paromomycin, and calculate
the 30SParomomycin binding energy to understand the details of the drug
interaction with their macromolecular target. Materials and Methods:
Using the Molecular Fractionation with Conjugate Caps (MFCC) method,
we calculate the binding energies of each individual nucleotide
encompassed within 16 sphere centered on the drug centroid. Results
and Discussion: We found A1408 (-18,3 Kcal/mol), G1491 (-16,9
Kcal/mol), G1405 (-15,4 Kcal/mol), U1495 (-14,7 Kcal/mol), G1494 (-11,8
Kcal/mol), C1407 (-11,2 Kcal/mol) and G1405 (-9,8 Kcal/mol) nucleotides
interacting strongly with the drug and, consequently, are indispensable for
drug-30S stability. Experimental data show the phenotype of drug
resistance of bacteria with single point mutations in these residues. The
present quantum sudy povdes mpon nsgh on specc bosome
drug interaction and should be quite useful in the design of new
mnogycosdes h hve supesong bndngs o he 30S.



160

CODE 153
SYNTHESIS AND CHARACTERIZATION OF AMOXICILLIN AND
METRONIDAZOLE-BASED CHITOSAN MICROPARTICLES

Carvalho, J.R.P.
1
; Freire, F.D.
1,2
, Morais, W.A.
3
; Raffin, F. N.
1,2

1
Universidade Federal do Rio Grande do Norte, Departamento de
Farmcia, Laboratrio de Desenvolvimento de Medicamentos (LDM)
Natal, RN.
2
Programa de Ps Graduao em Desenvolvimento e Inovao
Tecnolgica em Medicamentos (PPgDITM) UFRN.
3
Univesidade Federal do Rio Grande do Norte, Departamento de
Farmcia, Laboratrio de Farmacotcnica Natal, RN.
Corresponding author: joaoricardopc@hotmail.com

Introduction: Helicobacter pylori is a bacteria associated with diseases
such as peptic ulcer and gastric cancer. Amoxicillin and metronidazole are
antimicrobials of choice to treat H. pylori infections. The current treatment,
however, is made difficult mostly because of the insufficient gastric-
residence time of these drugs, in a way that effective concentrations are
not reached. Polymeric microparticulated systems are advantageous
because they are able to deliver drugs in a proper site of absorption and in
an appropriate amount. The use of natural polymers such as chitosan is of
great interest, because of its biocompatibility, degradability, non-toxicity
and mucoadhesion. Objetive: The aim of this study is to incorporate
amoxicillin and metronidazole in chitosan microparticles to be delivered to
the gastric mucosa, and also to evaluate drug entrapment efficiency and
granulometry. Methods: Simple coacervation method was used to
prepare the particles. Samples were prepared in different drug-polymer
ratios (1:1 and 1:2) and in different concentrations (0,25 and 0,5%). Drug
loading studies were carried out by UV/Vis spectrophotometry and size
distribution was done by optical microscopy (FERRET). Results: The
samples showed irregular surfaces and homogeneity in size distribution
(sizes varying from 5 to 20 m between all samples). Results for drug
entrapment efficiency showed that about 23% of amoxicillin was
successfully loaded into the particles (1:1 0,5%). For the metronidazole-
containing samples, the loading was about 16% (1:2 0,25%).
Conclusions: Results showed that higher proportions of the polymer as
well as higher concentrations led to better drug loading.



161

CODE 154
VISCERAL LEISHMANIASIS: SURVEY OF NOTIFIED CASES OF
DISEASE IN THE CITY OF ASSU / RN

Salgado, T. M. M.
1,3
; Silva, A. M. S.
1,3
; Oliveira, H. F. A.
1,3
; Dantas, M. G.
1,3
;
Barbosa, V. S. A.
2,3

1
Acadmico,
2
Docente
3
Curso de Bacharelado em Farmcia, Unidade Acadmica da Sade,
Centro de Educao e Sade, Universidade Federal de Campina Grande
(UFCG).
Corresponding author: maiacn@hotmail.com

Introduction: Visceral leishmaniasis (VL) is a serious chronic disease
potentially fatal to humans in which mortality may reach 10% when no
proper treatment is instituted. In Brazil, the importance of LV occurs not
only in its high incidence and wide distribution, but also the ability to take
severe and lethal when combined with the picture of malnutrition and
concurrent infections. Objective: This study aimed to make an
epidemiological survey of cases of visceral leishmaniasis in the period
February 2007 to July 2011, the city of Assu, situated in the State of Rio
Grande do Norte. Materials and Methods: We analyzed clinical and
epidemiological information recorded in databases of the Information
System for Notifiable Diseases (SINAN), the Health Department of the
Municipality and investigation forms for patients. Results and Discussion:
We confirmed by serology by ELISA and RIFI, 25 cases of the disease
(71.42%) of 35 reported, equating to an average of 5.5 cases / year. Most
affected were children (n = 15, 60%), predominantly male (n = 8; 53.33%).
The drug of choice for treatment was the pentavalent antimony. All patients
lived on the outskirts of the urban area. Between 2010 and 2011 samples
were collected from 260 dogs and 109 of these were positive (41.9%).
Were removed 83 dogs with positive serology and 72 without confirmation.
Conclusions: The combined data suggest better attention to the control of
disease, environmental sanitation, vector control, the reservoir hosts and
treatment of those infected.


162

CODE 156
OZAWA CALCULATION APPLIED TO THE KINETIC STUDY OF
DECOMPOSITION OF FLUCONAZOLE BY THERMOGRAVIMETRY

Azevedo, W. M. de
1
; Almeida, V. D. de
1
; Silva, E. P. da
1
; Souza, F. C. de
1
;
Arago, C. F. S.
2
; Gomes, A. P. B.
2

1
Undergraduate, Laboratory Quality Control (LCQMed), Pharmacy
Department (DFAR), Federal University of Rio Grande do Norte (UFRN).
2
Corresponding author: wendelmedeyros@hotmail.com

Introduction: Several studies have shown that thermogravimetry is a rapid
and convenient technique for the determination kinetic study of
decomposition reactions. Arrhenius equation and model Ozawa have been
used to calculate energy of activation and order of reaction from
thermogravimetric curves. Objectives: In this study, fluconazole raw
material and capsules were analysed by Ozawa model for dynamic TG
curves. Materials and Methods: Fluconazole raw material obtained from
Galena Qumica e Farmacutica Ltda was used in this study. Dynamic
thermogravimetric curves of fluconazole raw material were obtained using
a Shimadzu model DTG-60 thermobalance at 10, 20, 40, 60 and 80C.min
1
heating rates up to 900C (n=3) in a nitrogen (flow rate: 50 mL.min
1
). It
was weighted 7.00.5 mg in alumina crucible. The curves TG were
analyzed using TASYS program from Shimadzu, TGA Kinetic Analysis
Program (Ozawa Model). The following kinetic parameters by Ozawa
model kinetic energy, order and frequency factor. Discussion and
Results: The fluconazole showed the following kinetic parameters:
order(0.0), kinetic energy (84.88 KJ/mol) and frequency factor(1.78 x 10
7

min-1) fo 0.1, as the fluconazole capsules presented the following
kinetic parameters: order(0.1), kinetic energy (94.40 KJ/mol) and frequency
factor(3.16 x 10
9
min-1) fo 0.1. Fluconazole raw material has greater
thermal stability than the fluconazole capsules due has greater activation
energy. This shows that the excipients of the formulation may interfere with
the stability. Conclusions: Ozawa model can be used to determine the
parameters kinetics and stability of raw material and formulations.

FAPERN


163

CODE 157.1
THERMAL CHARACTERIZATION OF ALKALOID SYNTETIC WE014 BY
TG/DTA AND DSC

Silva, E. P.
1
; Galvo, F. P.
2
; de Arajo

Jnior, J. X.
4
; Frana, P. H. B.
4
;
SnAn, A. E. G.
4
; Arago, C. F. S.
3
; Gomes, A. P. B.
3

1
2
Posgraduate, LCQMed, DFAR, UFRN.
3
4
Professor, LPqRN/UFAL,ESENFAR, UFAL, Brazil.

Introduction: Thermal analytical methods are precise and accurate
techniques with low sample requirements, and can provide detailed
information about new chemical entities even at the very earliest stages of
discovery and development of the new compositions and drugs. Objective:
In this work, the alkaloid synthetic WE014 was investigated by means of
differential scanning calorimetry (DSC) and simultaneous
thermogravimetry/differential thermal analysis (TG/DTA). The results
allowed us to acquire information concerning these compounds in the solid
state, including their thermal stability and thermal decomposition. Materials
and Methods: The alkaloid WE014 was analyzed by TG/DTA on a
SHIMADZU thermobalance, model DTG-60, using an alumina crucible, at
10 C/ min
1
heating rate up to 900C, under an atmosphere of nitrogen at
50 mL/ min
1
. It was weighted 7.00.5 mg. The DSC curves were obtained
for SHIMADZU calorimeter, model DSC-60, using an aluminum crucible, at
10C/min
-1
heating rate up 450C. The curves were analyzed using TASYS
program from Shimadzu. Results and Discussion: The alkaloid WE014
showed four steps of decomposition. The first step corresponds the weight
loss the humidy, with 6.35%. The second step corresponds the
decomposition and onset temperature was 215.82, with weight loss about
15.83%. The percentage of ash corresponds to 3.26% at 900 C.The DSC
data showed a melting peak at 210.64 C, with onset at 207.69 C, Heat at
175.47 J/g and purity 97.22%. Conclusions: The TG/DTA and DSC can
be used in the determination of the parameters stability and purity for
pharmaceuticals.

FAPERN.


164

CODE 157.2
DETERMINATION OF KINETIC PARAMETERS BY OZAWA OF
ALKALOID SYNTETIC WE014

Galvo, F. P.
2
; Silva, E. P.
1
; Jnior, J. X. de Arajo
4
; Frana, P. H. B.
4
;
SnAn, A. E. G.
4
; Arago, C. F. S.
3
; Gomes, A. P. B.
3

1
2
Posgraduate, LCQMed, DFAR, UFRN.
3
4
Professor, LPqRN/UFAL,ESENFAR, UFAL, Brazil.

Introduction: Thermal analytical techniques can provide important
information regarding storage and stability of pharmaceuticals. Also, kinetic
parameters obtained from thermoanalytical data are highly useful for
making predictions of performance parameters of drugs, for example,
shef fe. The samples used in the analysis are the type guanilhidrazonas
aromatic alkaloids that reports of several guanilhidrazonas studied as
antihypertensives (Foye, 1990; Hoffman, 2003), antidiabetic (Larsen, 2001,
Edmonton, 2000), antineoplastic (Andreani, 2000; Foye, 1990; Brzozowski,
2007). Objective: The aim of this study was to determinate by Ozawa
model the kinetic parameters for WE014. Materials and Methods: The
curves TG alkaloid WE014 were obtained in the thermobalance
SHIMADZU, model DTG-60, using an alumina crucible, at 10, 20, 40, 60
and 80 C/ min
1
heating rate up to 900C, under an atmosphere of
nitrogen at 50 mL/ min
1
. It was weighted 7.00.5 mg. The curves TG were
analyzed using TASYS program from Shimadzu, TGA Kinetic Analysis
Program (Ozawa Model). Results and Discussion: The alkaloid WE014
was analysed by TGA kinetic analysis in the heating rate 10, 20, 40, 60 e
80 C min-1.The following kinetic parameters were found: kinetic energy
(110.51 KJ/mo), SD (4.17), CV(3.77%) fo 0.1, in the analysis range:
213.92-225.95C, in the calculation range: 91.11-86.30%. Conclusions:
The thermal analysis obtain results quickly and realible from non-emprical
data, meanly kinetic parameters.

FAPERN


165

CODE 158
DEVELOPMENT OF GOLD NANOPARTICLES CONTAINING
DOCETAXEL

Oliveira, R.
1,2
*; Barratt, G.
1
; Moine, L.
1
; Santa Maria, L.C.
2

1
Universit Paris Sud, UMR CNRS 8612, Facult de Pharmacie,
Chtenay-Malabry, France.
2
Universidade do Estado do Rio de Janeiro, Instituto de Qumica, Brazil.
Corresponding author: rachel.oliveira@u-psud.fr

Introduction: Prostate cancer is the third most important cancer in terms
of incidence and the sixth in terms of mortality for men throughout the
world. At the moment, it is treated with hormone therapy but this is only
effective for 24-36 months before the patients develop resistance. One of
the few active cytotoxic molecules is docetaxel (DOC) or Taxotere. The
encapsulation of this molecule into organo-metallic nanoparticles which
could target the cancer cells directly would be an original therapeutic
approach. Objective: The aims of this project are to formulate gold
nanoparticles carrying DOC, PEG and folic acid are a targeting ligand for
PSMA and to evaluate them in vitro and in vivo. Materials and Methods:
The gold nanoparticles were made by the Institut des Sciences
Molculaires in Bordeaux.
Nanoparticle characterization
Measurement of size and zeta potential by quasi-elastic light
scattering
Measurement of size and morphology by TEM
Evaluation of the ability of the folic acid on the particle surface to
bind to PMSA using the Biacore technique available at Chtenay-
Malabry.
Evaluation in vitro: The MTT was used to measure cell viability.
Results:

Particles
size (nm)
Zeta
Potencial
(mV)
nanoAu1 nanoAu2
nanoAu2
filtered*
nanoAu3
nanoAu3
filtered*
296,0
-22,2
121,8
-15,9
84,7
- 12,5
28,7
-6,1
25,0
-7,4

* The suspension containing the nanoparticles was filtered with a sterile
syringe filter 0.2 mM VWR cellulose acetate.
Conclusions: The results obtained so far indicate that gold nanoparticles
are promising as drug carriers for prostate cancer treatment.

Financial support from CAPES Brazil


166

CODE 160
EVALUATION IN VITRO OF SCHINUS TEREBINTHIFOLIUS
ANTIOXIDANT ACTIVITY BY CAPTURE OF FREE RADICAL 2,2-
DIFENIL-1-PICRILIDRAZIL (DPPH)

Silva, J. B. R.
1
; Amorim, A. F. C.
1
;Caldas, G. F. R.
1
; Nbrega, R. F.
1
; Silva,
J. L.
1
; Wanderley, A. G. W.
1,2
; Lafayette, S. S. L.
1,2

1
Departament of Pharmaceutical Sciences, Federal University of
Pernambuco (UFPE).
2
Department of Physiology and Pharmacology, Federal University of
Pernambuco (UFPE).

Introduction: Schinus terebinthifolius Raddi (Anacardiaceae) is used in
traditional Brazilian medicine due its properties adstringent, antidiarrheal,
diuretic, antibacterial, antipyretic, anti-inflammatory and antioxidant. Many
of these properties are associated to the presence of polyphenols.
Objective: Evaluate antioxidant activity in vitro of hydroalcoholic extract of
stem bark of Schinus terebinthifolius (ST) by capture of free radical 2,2-
difenil-1-picrilidrazil (DPPH). Materials and Methods: The antioxidant
activity was determined by the hydrogen-donating ability of ST. Aliquots of
1 mL ethanolic solution of DPPH (30 mmol/L) was mixed with 2.5 mL of
different concentrations of ST (2.5-250 g/mL). The absorbance was
measured after 10 min at 517 nm (Brand-Williams et al., 1995). The IC
50

(concentration of sample required to inhibit 50% of the radical DPPH) was
calculated. Data were express as means.e.m. Differences between
samples were analyzed by ANOVA nd Tukeys es (p < 0.05). The
synthetic antioxidant butilaldehyde hydroxytoluene (BHT) was used as
positive control. Results and discussion: Significant DPPH radical
scavenging activity was evident in all concentrations tested. ST was able to
reduce the stable free radical DPPH with an IC
50
8.5 g/mL. The BHT, in
similar conditions showed IC
50
73.8 g/mL. Oxidative stress due to an
increase in free radical generation or an impaired endogenous antioxidant
mechanism is an important factor that has been implicated in various
diseases. Conclusions: Schinus terebinthifolius showed significant activity
in the capture of DPPH radical, showing a high antioxidant activity,
probably related to its content of flavonoids. The clinical relevance of these
findings in requires further studies.

Financial Support from FACEPE/Brazil.


167

CODE 161.1
ANTIMICROBIAL ACTIVITY OF Annona coriacea L. AGAINST ORAL
CAVITY BACTERIA

Silva, K. M. A.
1
; Alencar, L. C. B.
1
; Clementino, E. L. C.
1
; Santana, C. P.
1
;
Fernandes, F. H. A
1
; Brito, E. M. C.
1,2
1,2*

1
Laboratrio de Desenvolvimentos e Ensaios de Medicamentos, Centro de
Cincias Biolgicas e da Sade, Universidade Estadual da Paraba,
Campina Grande PB.
2
Estadual da Paraba, Campina Grande PB.
Corresponding author: anaclaudia@uepb.edu.br

Introduction: Annona coriacea L. is a plant popularly used in the treatment
of inflammatory processes, its leaves, seeds and roots are used for this
purpose. Although known its effect during inflammatory processes, there
are few studies of other medicinal properties of this plant. Objective: To
investigate the antimicrobial properties of this plant on cariogenic and oral
biofilm-forming bacteria. Methods: The hydroalcoholic extract of the A.
coriacea bark was tested by the broth microdilution method, against the
strains of oral biofilm: Streptococcus mutans (ATCC 25175), S. oralis
(ATCC 10557), S. salivarius (ATCC 7073) and Enterococcus faecalis
(ATCC 29212). The tests were performed in triplicate. Results and
discussion: The screening carried out showed that the concentration of
100% of the A. coriaecea L. extract was able to inhibit the growth of S.
oralis, S. salivarus and E. faecalis with the same concentration, which
showed bactericidal effect. The organism S. mutans, in turn, showed a
greater sensitivity to the extract, which was inhibitory at the concentration
of 25% and bactericidal at the concentration of 50%.
Conclusion: A. coriacea L. may interest to dentistry, since, in addition to
its known anti-inflammatory properties, also proved its antimicrobial
activity. Thus, other tests would allow the use of this plant in the treatment
of oral cavity injuries, optimizing its effectiveness and ensuring the safety of
treatment.

Financial support from FAPESQ/MCT/CNPQ and PROPESQ/UEPB


168

CODE 161.2
MINIMUM INHIBITORY CONCENTRATION OF Acanthospermum
hispidum DC FRONT OF Streptococcus oralis BYTHE
MICRODILUTION AND AGAR DIFFUSION METHODS

Silva, K. M. A.
1
; Santos, R. L.
1,2
; Chaves, T. P.
1
; Santana, C. P.
1
;
Fernandes, F. H. A.
1
; Brito, E. M. C.
1,2
1,2*

1
Campina Grande PB.
2

Introduction: Acanthospermum hispidum DC popuy known s Espnho
de Cgno s wdey used n northeastern Brazil, in the treatment of
asthma, bronchitis, as antipyretic, expectorant, vermifuge and against
intestinal aches.Objective: To determine the minimum inhibitory
concentration (MIC) of Acanthospermum hispidum DC extract by agar
diffusion method using the technique of the cylinder and microdilution in
broth front of Streptococcus oralis. Materials and Methods: Extracts were
obtained by maceration process followed by rotative evaporation. From
these extracts we obtained ten dilutions of the starting concentration of
1mg/ml, diluted in DMSO (10%). These were tested against Streptococcus
oralis (ATCC 10557) by the agar diffusion method and dilution in
microplates. Results and Discussion: The extract of Acanthospermum
hispidum DC showed no antimicrobial activity in any of the concentrations
on Streptococcus oralis by microdilution method, however, by the agar
diffusion method it was active with inhibition halos above 10 mm in all
dilutions tested, since the concentration of 1 mg / ml to 0,05 mg/ml.
Conclusions: The extract of Acanthospermum hispidum DC, when tested
against strains of Streptococcus oralis, showed inhibition of bacterial
growth only by agar diffusion method. Therefore, this method obeys Fick's
Law, and proved to be more efficient.

Financial support from FAPESQ/MCT/CNPQ and PROPESQ/UEPB.


169

CODE 162.1
ANTIMICROBIAL ACTIVITY OF Acanthospermum hispidum DC. BY
MICRODILUTION

Alencar, L.C.B.
1
; Chaves, T. P.
1
; Nbrega, F. P.
1
; Brando, D. O.
1
;
Barbosa, A. S.
1
; Arajo, R. M.
1
1,2*

1
Campina Grande PB.
2

Introduction: Acanthospermum hispidum is a widely used plant in
traditional medicine around the world to combat various types of infections.
Since there are differences in relation to parts of the plant to be used, it is
necessary to investigate if there are differences in the antimicrobial
potential of these parts.
Objective: To evaluate and compare the antimicrobial activity of root
extract of A. hispidum, in relation to the entire plant extract. Methods: The
hydroalcoholic extracts (70%) of the root and the whole A. hispidum,
obtained by maceration, were tested by broth microdilution method, in front
of microbial strains: Escherichia coli (ATCC 25922), Staphylococcus
aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853) and
Klesbisiela pneumoniae (ATCC 4352). Three repetitions were performed.
Results and discussion: The screening carried out showed that both
extracts were effective against S. aureus, P. aeruginosa and E. coli. The
root extract showed bactericidal effect against P. aeruginosa and inhibitory
against E. coli in a concentration less than the entire plant extract, while
the inhibitory concentrations and bactericidal against S. aureus were the
same in both extracts. There was no activity of the extracts against K.
pneumoniae. Conclusion: Considering the results obtained, we can affirm
that the root extract of A. hispidum was more effective against the strains
tested. It is recommended to carry out further tests with this plant, to
ensure its safe use and most effective way possible.



170

CODE 162.2
MICROBIOLOGICAL COMPARISON OF Hyptis mutabilis Briq ACTION,
BY THE MICRODILUTION AND AGAR DIFFUSION METHODS

Alencar, L. C. B.
1
; Chaves, T. P.
1
; Fernandes, F. H. A.
1
; Arajo, R. M.
1
;
Santos, R. L.
1,2
; Barbosa, A. S.
1
1,2*

1
Campina Grande PB.
2

Introduction: Hyptis mutabilis Briq belongs to Lamiaceae family, is
commony known s Afvc-de-Cboco nd popuy used s n-
inflammatory, antiseptic, antipyretic, antispasmodic and antiflatulent.
Objective: To compare the antimicrobial action of H. mutabilis Briq by the
method of agar diffusion and microdilution in broth. Materials and
Methods: The hydroalcoholic extract (70%) of the whole plant was
obtained by maceration. This extract was tested against the strains
Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923),
Pseudomonas aeruginosa (ATCC 27853) e Klesbisiela pneumoniae
(ATCC 4352), by agar diffusion (technique of cylinder) and microdilution in
broth. Results and Discussion: The hydroalcoholic extract of H. mutabilis
showed no microbiological activity against any of the strains tested by agar
diffusion, but was active against all strains by the method of dilution in
microplates, with a minimum inhibitory concentration (MIC) of 6.25% and a
minimum bactericidal concentration (MBC) of 12.50% to S. aureus, 50.00
% and 100.00% of MIC and MBC respectively for Klesbisiela pneumoniae
and Pseudomonas aeruginosa. To Escherichia coli MIC was 25.00 % and
MBC 50.00%. Conclusions: Although the agar diffusion method, using the
technique of the cylinder, is considered more analytical, this method did not
show satisfactory results against the strains mentioned, while the
microdilution tests showed a higher sensitivity.



171

CODE 163.1
DIAGNOSIS OF HYGIENIC-SANITARY CONDITIONS AND
COMPLIANCE WITH GOOD MANUFACTURING PRACTICES IN FOOD
SERVICE AT PUBLIC DAY CARE CENTERS IN CAMPINA GRANDE-PB

Ramos Jnior, F. J. L.
1
; Ribeiro, J. L.
2
; Brando, D. O.
3

1
Discente de graduao, Departamento de Farmcia, Universidade
Estadual da Paraba (UEPB).
2
3

Corresponding author: fjdlrjunior@gmail.com

Introduction: Food borne Diseases (FBD) are clinical events resulting
from ingestion of foods that may be contaminated, being the main sites of
outbreaks of FBD the day care centers and the schools. Objective:
Diagnose the sanitary-hygienic conditions in the services of food of day
care centers and public schools of Campina Grande-PB. Materials and
Methods: The study was carried out in a sample of some day care centers
and schools. It was elaborate a check-list based on the Resolution
RDC/ANVISA N. 275 and RDC/ANVISA N. 216. Besides, it was analyzed
the microbial contamination of the water used in the preparation of foods,
using the technique of the multiple tubes. The determination of mesophilic
aerobic bacteria, recommended by Ordinance No. 518, of the Ministry of
Health, was carried out according to standard methods by APHA (1955).
Results and Discussion: Only 16,66 % of the institutions attended more
than 75 % of the evaluated items being classified like Group 1; other 50 %
obtained service between 51-75 % being classified like Group 2 and 33,33
% attended less than 50 % fits into Group 3. It was noticed that the number
of samples in accordance with the microbiological criteria was higher in the
institutions that had better service to the checklist of checking of the
conditions sanitary-hygienic. Conclusion: The institutions reached a low
level of service regarding the Good Practices of Manufacture, according to
directives of the legislation, which demonstrates unsatisfactory sanitary-
hygienic conditions for food production in these institutions..



172

CODE 163.2
PREVALENCE OF INTESTINAL PARASITES AMONG STUDENTS OF
PUBLIC DAY CARE CENTER IN THE CITY OF CAMPINA GRANDE-PB

Ramos Jnior, F. J. L.
1
; Rodrigues, T. P.
2
; Medeiros, J. S.
3

1
2
3
Professor Doutor, Departamento de Farmcia, Universidade Estadual da
Paraba (UEPB).
Corresponding author: fjdlrjunior@gmail.com

Introduction: Intestinal parasitic diseases are caused by helminths and
protozoa that attack the intestinal tract of living things and, of the child,
interfere with the proper development. Objectives: Identify intestinal
parasites in children from a public day care center in the city of Campina
Grande-PB e verify the presence of parasitic helminth structures in soil
samples of the day care center. Materials and Methods: The study was
descriptive and experimental, conducted through the application form and
laboratory analisys on a sample of 79 students. The examinations were
performed at the Laboratory of Parasitology of State University of Paraba,
using direct methods, of Hoffman, Pons and Janer, Rugai, Mattos e Brisola
and the method of Graham. The soil was analyzed according to the
method of Willis, adapted for research into soil. The survey of nematode
larvae was performed using the technique of Rugai, Mattos and Brisola,
with the adjustment proposed by Carvalho et al. The data were submitted
to the Epi Info version 3.4. Results: Of the samples analyzed, 62% had
some type of parasite, where the male gender the highest positivity (65%).
The most prevalent species were Giardia lamblia, Blastocystis hominis,
Ascaris lumbricoides, Entamoeba histolytica/Entamoeba dispar,
Entamoeba coli and Trichuris trichiura. The soil samples had larvae that
were classified as larvae of nematodes. Conclusion: Due to the
epidemiological and strategic importance of this research, we suggest a
larger number of such studies, aimed to standardize methodologies for
gathering and analysis, targeting other areas at risk of environmental
contamination.



173

CODE 164
FORMULATION AND EVALUATION OF SUNSCREEN EMULSIONS
USING EXPERIMENTAL DESIGN

Neves, J. K. O.
2
; Apolinrio, A. C.
1
; Silva, D. T. C.
1
; Soares, B. S. A.
1
;
Santos, D. P. G.
1
; Vras, G.
3
; Silva, J. A.
1,2

1
Departamento de Farmcia /Universidade Estadual da Paraba/UEPB,
Campina Grande, PB.
2
Programa de Ps-Graduao em Cincias

Farmacuticas/Universidade
Estadual da Paraba/UEPB, Campina Grande, PB.
3
Programa de Ps-Graduao em Cincias

Agrrias/Universidade
Estadual da Paraba/UEPB, Campina Grande,PB.
Corresponding author: alexuepb@yahoo.com.br

Introduction: Exposure to sunlight provides beneficial and essential
effects, but excessive exposure to ultraviolet radiation can cause skin
photo aging, erythema and different skin cancer. However, those effects
can be prevented by the use of sunscreen emulsions. Objective: The aim
of this work was to apply factorial design methods to reduce the number
and time of the various steps required to formulate and evaluate stable
sunscreen emulsions. Materials and Methods: Eight formulations were
prepared according to a 2
3
factorial design employing the following factors
and levels: Emulsifying agent type (Polawax
and Cosmowax
),
emulsifying agent concentration (10 and 12 %) and emollient type
(isopropyl myristate and decyl oloeate). The emulsion stability was
estimated by the average size of the droplets, the emulsion spreadability
and the emulsion conductivity and pH. The emulsions were prepared by
the phase inversion technique. Results and Discussion: The average
size of the droplets was influenced by factors emulsifying agent
concentration and emollient type. According to the fact that minimum
droplet diameter corresponds to the most stable emulsion, the increase in
the amount of emulsifying agent and the use of emollient decyl oleate
resulted smaller droplets. The emulsion conductivity was influencied by
emulsifying agent type, emulsions made with Polawax
showed a higher
value. In case of the emulsion spreadability and pH, the factorial design
cannot be used for pharmacotechnique optimization of formulations
because the values found were not altered by the factorial matrix.
Conclusion: Experimental design allowed to optimize
important parameters involved in the formulation of sunscreen emulsions.


174

CODE 165.1
INTRINSIC DISSOLUTION: THE INFLUENCE OF THE TECHNIQUE ON
THE KINETIC PARAMETERS EVALUATION OF ANTIRETROVIRAL
EFAVIRENZ

Moura, M. L. C1; Vilarinho, A. C. S. G.2; Nunes, L. C. C. 2; Alves, L. D.
S.1; Soares, M. F. R.1,2; Sobrinho, J. L. S.1; Rolim-Neto, P. J.1

1
Federal University of Pernambuco. Recife, PE, Brazil.
2
Federal University of Piaui. Terezina, PI, Brazil.
Corresponding author: marialuizacmoura@hotmail.com

Introdution: The intrinsic dissolution rate (IDR) is defined as the
dissolution rate of pure substances under the condition of constant surface
area. This dissolution can be done by two ways: rotating and stationary
disk. Objective: This research aimed evaluates the difference between the
two forms of intrinsic dissolution in the evaluation of the kinetic parameters
of the antiretroviral drug efavirenz (EFV). Materials and Methods:
Weighed 20mg of EFV, which was inserted in the cavity of the die (intrinsic
dissolution apparatus) to be compressed for a hydraulic press. The intrinsic
dissolution were performed in different times and constant pressure. All
used the medium sodium lauryl sulfate (SLS) 0.5% and speed of 50 rpm.
Results and Discussion: From the results obtained it was noted that the
drug had a better dissolution profile in the method with stationary disk. We
can correlate the results with the possibility of air bubbles formation and
the differences on the hydrodynamic. Conclusion: Based on the above
can be said that the intrinsic dissolution by stationary disk measure more
accurately the drug dissolution of the EFV.


175

CODE 165.2
INTRINSIC versus APPARENT DISSOLUTION: INFLUENCE OF
COMPRESSION ON THE KINETIC EVALUATION OF

Moura, M. L. C.
1
; Vilarinho, A. C. S. G.
2
; Nunes, L. C. C.
2
; Alves, L. D. S.
1
;

Soares, M. F. R.
1,2
; Sobrinho, J. L. S.
1
; Rolim-Neto, P. J.
1

1
Federal University of Pernambuco. Recife - Brazil.
2
Federal University of Piaui. Terezina - Brazil.
Corresponding author: marialuizacmoura@hotmail.com

Introduction The dissolution test is a in vitro predictive method to measure
the drug availability in vivo. Objective To compare the effectiveness
between the apparent and intrinsic dissolution test using the antiretroviral
drug efavirenz (EFZ). Materials and Methods The EFV apparent
dissolution was realized with apparatus 2 (paddle) and 10 mg EFV
capsules. The intrinsic dissolution was realized with 20 mg EFZ tablets by
a hydraulic press, in a stationary die. Before and after of compression was
done x-ray diffraction (RXD) to evaluate the integrity of the EFV crystal
form, drug that has polymorphism, and with bioavailability variant due to
this. Both dissolution were done in the following conditions: 37C, 50 rpm,
medium with sodium lauryl sulfate 0.5%, sextuplicate and collect time of 15
and 60 minutes. Results and Discussion The results obtained showed
that the intrinsic dissolution evaluated the drug performance more
accurately when compared to the apparent dissolution (Qap=60.21% and
Qint=14.68%, 60 min), however from the XDR can be seen that the
compression interfered in the crystallinity of the drug, which could generate
polymorphic forms of pressure, which affects the outcome in vitro and,
consequently, in vivo of the EFV.


176

CODE 167
INVESTIGATION OF THE SPASMOLYTIC ACTION OF Solanum
stramonifolium Jacq. ON RAT AORTA AND GUINEA-PIG ILEUM

Silva, M. C. C.
1
; Pereira, J. C.
1
; Correia, A. C. C.
2
; Martins, I. R. R.
2
; Souza,
I. L. L.
1
; Oliveira, G. A.
2
; Travassos, R. A.
2
; Silva, T. M. S.
3
; Agra, M. F.
4
;
Silva, B. A.
4

1
Lboo de Tecnoog Fmcuc Pof. Deby Fenndes de
Medeos, Unvesdde Fede d Pb, Joo Pesso/PB, Bazil.
2
Programa de Ps-graduao em Produtos Naturais e Sintticos
Bioativos, Universidade Federal da Paraba, Joo Pessoa/PB, Brazil.
3
Departamento de Qumica, Universidade Federal Rural de Pernambuco,
Recife/PE, Brazil.
4
Departamento de Cincias Farmacuticas, Universidade Federal da
Paraba, Joo Pessoa/PB, Brazil.

Introduction: Solanum genus (Solanaceae) is constituted of several
species, including Solanum stramonifolium Jacq. which was chosen based
on chemotaxonomic criterion since other species already showed
spasmolytic action. Objective: perform a comparative study of crude
ethanolic extract of green (SS-EtOH
FV
) and ripe (SS-EtOH
FM
) fruits of S.
stramonifolium for a possible spasmolytic activity on rat aorta and guinea-
pig ileum. Methodology: organs were suspended in organ baths in
appropriate conditions. Isotonic and isometric contractions were monitored
and recorded. All the experimental protocols were approved by Ethical
Committee in Animal Research of LTF/UFPB (Protocol 0506/05). Results:
SS-EtOH
FV
and SS-EtOH
FM
(243 and 729 g/mL) showed no significant
spasmolytic activity on rat aorta pre-contracted by phenylephrine in both
absence and presence of functional endothelium. On guinea-pig ileum,
SS-EtOH
FV
and SS-EtOH
FM
(243 and 729 g/mL) inhibited significantly the
phasic contractions induced by histamine (E
max
= 64.3 1.0 and
61.1 4.7%, respectively). Interestingly, when the agonist used was
carbachol, a different effect was observed: whereas SS-EtOH
FV
(243 and
729 g/mL) inhibited significantly the phasic contractions
(E
max
= 67.2 10.5%), in the other hand SS-EtOH
FM
inhibited the phasic
contractions in a significant and concentration dependent manner
(IC
50
= 217.5 31.6 g/mL). Conclusions: Both extracts have not
vasorelaxant effect in rat aorta. However, SS-EtOH
FM
showed a better
spasmolytic activity than SS-EtOH
FV
on guinea-pig ileum. Thus, it is
suggested that the fruit ripeness degree affected the pharmacological
effect of the tested extracts, possibly due to qualitative and quantitative
changes of S. stramonifolium secondary metabolites.

Financial Support from CNPq/CAPES.


177

CODE 168
KINETIC STUDIES FOR ITRACONAZOLE USING OZAWA MODEL BY
DYNAMIC THERMOGRAVIMETRY

Almeida, V. D. de
1
; Azevedo, W. M. de
1
; Silva, E. P. da
1
; Souza, F. C. de
1
;
Arago, C. F. S.
2
; Gomes, A. P. B.
2

1
2
Corresponding author: vanessanill@yahoo.com.br

Introduction: Thermogravimetry (TG) is a thermoanalytical technique
used in pharmaceutical studies for the characterization, purity, compatibility
of formulations, the identification of polymorphism, stability assessment. In
previous studies to characterize the kinetics of thermal decomposition were
used the Ozawa model for curves non-isothermal and Arrhenius equation
for isothermals. Objective: Evaluate application of the Ozawa model for
characterization of thermal decomposition kinetic of Itraconazole at
dynamic TG curves. Materials and Methods: The Itraconazole raw
material coated pellets analyzed was obtained from Farmafrmula
Manipulation Pharmacy. For kinetic determination, non-isothermal TG
curves were obtained using a Shimadzu thermobalance, model DTG60.
Alumina crucible with 10.5 mg (0.5) of sample under a nitrogen flow at 50
mL.min
1
and heating rates of 10, 20, 40, 60 and 80C.min
1
up to 900C
(n=3) was used for the process. The kinetic parameters were determined
via Ozawa model and Arrhenius equation. Discussion and Results: The
itraconazole showed the following kinetic parameters: order(0.3), kinetic
energy (99.09 KJ/mol), SD (1,27), CV(1,35%) and frequency factor(1.02 x
10
10
min-1) fo 0.1, as the itraconazole capsules presented the
following kinetic parameters: order(0.3), kinetic energy (95.56 KJ/mol), SD
(2,08), CV(2,18%) and frequency factor(1.02 x 10
10
min-1) fo 0.1.
Itraconazole raw material has greater thermal stability than the itraconazole
capsules due has greater activation energy. This may indicate interactions
with excipients in the formulation since they should improve stability.
Conclusions: Through the application of the Ozawa model can determine
the stability of pharmaceuticals and raw materials.

FAPERN


178

CODE 169
PHYTOCHEMICAL STUDY AND ANTI-INFLAMMATORY ACTIVITY OF
AQUEUS EXTRACTS AND FRACIONS OF MIMOSA TENUIFLORA
(JUREMA PRETA) AND HANCORNIA SPECIOSA (MANGABEIRA)

Bitencourt, M. A. O.
1
; Lima, M. C.
1
; Torres, M.
1
; Furtado, A.
1
; Langassner,
S. M. Z.
2
; Fernandes-Pedrosa, M. F.
1*

1
Laboratrio de Tecnologia e Biotecnologia Farmacutica, Departamento
de Farmcia, Universidade Federal do Rio Grande do Norte (UFRN).
2
Laboratrio de Farmacognosia, Departamento de Farmcia,
Corresponding author: mpedrosa@ufrnet.br

INTRODUCTION: The species Mimosa tenuiflora and Hancornia speciosa,
are plants used in traditional medicine for various diseases. Due to lack of
scientific studies, it is necessary to conduct studies to characterize the
active compounds of the species. OBJECTIVE: Within this context, the
objective of this work was the preliminary analysis of the majority
compounds and anti-inflammatory activity of aqueous extracts and
fractions of H. speciosa and M. tenuiflora. The decoction was subjected to
a liquid-liquid partition with solvents of increasing polarity: dichloromethane
(3x300mL), ethyl acetate (3x300mL) and n-butanol
(3x300mL). MATERIALS AND METHODS: The phytochemical fractions
were performed by Thin-Layer Chromatography (TLC). To evaluate the
anti-inflammatory activity, we essayed the model of against carrageenin-
induced paw edema and peritonitis. RESULTS AND DISCUSSION:
Analysis by TLC was observed flavonoid fractions of ethyl acetate and n-
butanol in H. speciosa, and dichloromethane and n-butanol in M.
tenuiflora. In the paw edema model were tested three doses (4, 20 and
40mg/kg), revealing a significant inhibition of edema in the fourth hour. For
the bioassay-guided fractionation is used the model of peritonitis where
hee ws sgnfcn educon eukocyes mgon n nms eed wh
the aqueous extracts at all three doses tested (20, 30 and 40mg/kg), and
fractions dichloromethane, and ethyl acetate. CONCLUSION: In parallel,
the bioassay-guided fractionation is being developing to characterize the
active compounds of the aqueous extract of H. speciosa and M. tenuiflora.

Financial Support from CNPq and CAPES.


179

CODE 170
THERMAL ANALYSIS MULTCOMPONET COMPLEX WITH -CD AND
METHOTREXATE

Barbosa, J. A. A.
1
; Oliveira, A. R.
1
; Melo, P. N.
1
; Rocha, H. C. S.
2
; Oliveira,
A. G.
3
1

1
Programa de Ps-Graduao em Cincias Farmacuticas. Universidade
Federal do Rio Grande do Norte, UFRN, Natal- RN.
2
Curso de Farmcia, UFRN, Natal- RN.
3
Faculdade de Cincias Farmacuticas de Araraquara, UNESP,
Araraquara-SP.

Introduction: Methotrexate (MTX) is a drug widely used in psoriasis, but
its systemic use may cause several side effects. An available alternative is
a topical drug delivery, which lead to increase the drug permeation and its
bioavailability in the region of damaged skin. However, topical formulation
requires good drug solubility.Thus, cyclodextrins are excipients that can
form soluble complexes and in association with hydrophilic polymers or
hydrophilic substances may present a synergistic effect in the icrement of
drug solubility. Objective: This study aimed to characterize the inclusion
complexes with MTX obtained by freeze-drying. Materials and Methods:
The TG curves were obtained using thermal balance in the range 0 to 450
C with a heating rate of 10 C min
-1
, submitted under an atmosphere of N
2

(50 ml.min
-1
) with aluminum pan. The samples were pure -CD, pure MTX,
physical mixture the compounds and the ternary complexes containing
polymers PEG 10000, Polaxamer 407, PVA, PVP and the hydrophilic
compound TEA. Results and Discussion: Accodng o TG of -CD occur
a first event at about 61- 103C with mass loss 10%, corresponding to
evaporation of adsorbed water and crystallization, and second event took
place at about 314-350 C with mass loss 66% , representing of
decomposition temperature. The curve of MTX the first event occurred at
about 75-117 C with mass loss 4%, characteristic of phase transition of
MTX due to loss of water of crystallization of its structure, and a second
event at 236-286 C with mass loss of 31%, characteristic of
decomposition of the drug. To suggest the formation of inclusion
complexes using a TG its necessary to compare the temperature of the
beginning of degradation of the drug alone and with the complex, and
complexes degradation occurs in higher temperatures. The SEM images
showed wo cys ypes fo MTX nd egu fom o -CD. The
inclusion complexes presented different forms, suggesting the obtention of
inclusion complexes. The XRD results demonstrated the different pattern of
the pure compounds suggesting the obtaining of binary and ternary
complexes with drug in amorphous phase. Conclusion: The events can
suggest complex form, however, it is important to complement various
other techniques that confirm the formation of complexes.

Financial support: CAPES and CNPq


180

CODE 171
PSEUDO-TERNARY PHASE DIAGRAMS AS A TOOL TO PRODUCE
EMULSION E MICROEMULSION

Silva, D.T.C.
1
; Apolinario, A.C.
1
; Souza, M.S.R.
1
; Fernandes, J.J.G.
1
,
Damasceno, B.P.G. L.
2
; Silva, J.A.
2

1
Universidade Estadual da Paraba (UEPB), Laboratrio de
Desenvolvimento e Caracterizao de Produtos Farmacuticos (LDCPF),
58429-600, Campina Grande-PB-Brazil.
2
UEPB, Programa de Ps-Graduao em Cincias Farmacuticas
(PPGCF), LDCPF, 58429-600, Campina Grande-PB-Brazil,
Corresponding author: alexuepb@yahoo.com.br

Introduction: The pseudo-ternary phase diagram construction (PTPD) is
an essential step to emulsions (E) and microemulsions (ME) production.
The PTPD describes the experimental condition in which it is possible to
obtain microemulsions and transition boundary
regions between emulsions, phases separated and microemulsions O/ A
and A / O. ME is isotropic, transparent and thermodynamically stable
systems. They are usually produced with 4 ingredients: water, oil,
surfactant and co-surfactant, in the PTPD, so the aqueous phase, oil
phase and mixture of surfactant / co-surfactant are represented at the
vertices of the triangle. This type of diagram also supports other mixtures,
such as oil, co-surfactant and water /surfactant. Objectives: Apply the
PTPD as a tool to obtain stable E and ME. Materials and Methods: The
PTPD were produced by the mixture of Plurol Oleique (CS) and
Labrasol (S) [2:8; 4:6; and 6:4, respectively], with the Captex 355 [oil
phase (O)] in the range from 1:9 to 9:1. To this S/CS/O mixture, the
aqueous phase (water Milli-Q) was titrate with an automatic pipette, and
shaken with magnetic stirrer. Results and Discussion: Two regions of
cosmetic and pharmaceutical interest were found: ME and semisolid E.
The optically transparent liquid systems were considered ME, on the other
hand, the viscous and opaque systems were called semisolid E.
Conclusion: These results allow us to conclude that PTPD is a good,
simple and reliable technique to characterize the area of E and ME
regions.


181

CODE 172
DETERMINATION OF MINIMUM INHIBITORY CONCENTRATION OF
HYDROALCOHOLIC EXTRACT PLANT ON CANDIDA SPECIES OF
MEDICAL INTEREST

Batista, R. S. A.
1
, Silva, G. S.
2
, Machado, S. E. F., Feitosa, I. L. F. F.,
Vieira, K. V. M.
3
, Dantas, I. C.
4
, Felismino, D. C.
5

Laboratrio de Microbiologia, Departamento de Farmcia, Universidade
Estadual da Paraba (UEPB);
Laboratrio de Microbiologia, Departamento de Farmcia, Universidade
Departamento de Farmcia, Laboratrio de Microbiologia, Universidade
4
Departamento de Farmcia, Laboratrio de Botnica, Universidade
5
Departamento de Biologia, Laboratrio de Botnica, Universidade
Corresponding author: e-mail: rayannesales@hotmail.com

Introduction: The use of medicinal plants has been widely seen as a
normative basis for the maintenance of health. Objective: To determine
the minimum inhibitory concentration (MIC) of hydroalcoholic extracts of
Lippia sidoides Cham, Punica granatum L. and Syzigium aromaticum L.
front of the Candida species related to vulvovaginal candidiasis (VVC) and
oral candidiasis (OC), Candida albicans (ATCC 18804), C. parapsilosis
(ATCC 22019), C. krusei (ATCC 34135), C. tropicalis (ATCC 13803), C.
guilhermondii (ATCC 6260). Materials and Methods: First, the strains
were grown; then, they were suspended in saline on the standard scale of
0.5 Mac Farland. The MIC was determined by agar diffusion technique,
cavity method. From the crude extract (100%), there were serially diluted in
sterile saline to 50%, 25%, 12.5%, 6.25%. In each hole, it was deposited
50L of ech duon. They wee ncubed 37C/24-48h. The extracts
were considered active when showed growth inhibition halos with
diameters bigger than 10mm. Results and Discussion: Of the extracts
tested, dilutions of the extract of Lippia sidoides Cham showed the
inhibition against strains of C. krusei and C. tropicalis, determining the 25%
dilution and the MIC (11mm halo), and the extract of Syzigium aromaticum
L. which showed inhibition against the strain of C. guilhermondii, dilution
50% (10.5 mm halo). Conclusion: It was observed that the plant species
Lippia sidoides Cham showed antifungal activity, prompting further
research on it, using preliminary data in determining the future of herbal
medicine against Candida species causing VVC and OC.

Financial support from PIBIC/UEPB


182

CODE 174
THE CURINE ANTIINFLAMMATORY EFFECT IS A CYTOKINE
DEPENDENT MECHANISM.

Carvalho, F. L. (PG); Alves, A. F*.; Ferreira, L. K. D. P.; Costa, H. F.,
Piuvezam, M. R. (PQ).

Departamento de Fisiologia e Patologia, Centro de Cincias da Sade.
UFPB/PB
Corresponding author: *adrianofalves@gmail.com

Introduction: Curine is a bisbenzylisoquinoline alkaloid from
Chondrodendron platyphyllum (Menispermaceae) and inhibited cell
migration to inflammatory site. Objective: The goal of this study was to
demonstrate the antiinflammatory mechanism of curine. Material and
Methods: Swiss mice (n=6-7) were treated with saline, curine (2.5 mg/kg)
or dexamethasone (2.0 mg/kg) and one hour later was administered
carrageenan or bradykinin in the left paw and the edema was measured by
paquimeter. The vascular leakage was evaluated by injection (i.p.) of acetic
acid solution into the peritoneum and amount of protein was measured by
spectrophotometer. The inflammatory cell migration and
cytokine/chemokine production (TNF-, IL-1, IL-6, CXCL-1 and MCP-1)
was analyzed into the pleural fluid of animals previously challenge with
LPS by citometric assay. Statistical analyses were performed by one-way
ANOVA. Results and Discussion: The curine treatment inhibited edema
formation induced by carrageenan but did not by bradikinin. Also curine
inhibited (p<0.05) vascular leakage and LPS-induced polymorphonuclear
migration (P<0.05) as compared with the control group. In addition the
secretion of TNF-, IL-6 and MCP-1 but not IL-1 was inhibited (P<0.05) by
curine. Dexamethasone reduced edema formation as well as inhibited the
cytokine production as expected. Therefore the present study
demonstrated that curine treatment regulates inflammation by inhibiting
cytokine and chemokine secretion as well as neutrophil migration to the
site of inflammation. Conclusion: The data suggest that curine is a potent
antiinflammatory drug by inhibiting the cell migration through a mechanism
dependent of MCP-1, TNF- and IL-6 production.

CAPES/Brazil


183

CODE 175.1
DTA/TGA/DTG ANALYSIS OF Euterpe oleracea (AA) GLYCOLIC
EXTRACT

Rodrigues, R. O.
[1]
; Daher, C. C.
[2]
; Fontes, I. S.
[1]
; Soares, D. S.
[1]
; Silva,
E.P.
[3]
; Gomes, A. P. B.
[3]
; Ferrari, M.
[2]
; Arago, C. F. S.
[3]

1
Laboratory of Research and Development of Cosmetic Products - Faculty
of Pharmacy, Federal University of Rio Grande do Norte.
2
Faculty of Pharmacy - Potiguar University
3
Laboratory of Quality Control Faculty of Pharmacy, Federal University of
Rio Grande do Norte.
Corresponding author: rayllanrodrigues@yahoo.com.br

Introduction: Euterpe oleracea is a specimen of Amazon composed of
polyphenols whose antioxidant activity is used in cosmetics preparations
with benefits for skin. Thermal methods and equipment related have been
improved in the past decade and logically their application is currently
much wider than previously. The thermals methods are tecnics used in
cosmetics for to charaterize actives, pre-formulation studies, thermal
stability studies and purity. Objectives: The aim of this work was to evaluate
the thermal behavior of aa glycolic extract. Materials and Methods: The
aa glycolic extract was analyzed by TG/DTA. The TG curves were
obtained with a Shimadzu thermobalance (DTG-60), under a N
2
flow 50
mL.min
-1
, at heating rate 10
o
C.min
-1
, up to temperature of 900C. Sample
mass about 8mg were packed in an alumina cell. TG/DTA curves was
analyzed with the aid of SHIMADZU software TASYS. Results and
Discussion: TGA curve reveled two stages in range of 45 - 73C (-35%)
and second at 119 - 138C (-62%). With 3% of the residue. DTG data
showed -0,85 mg and -3.34 mg. DTA curve show two endothermic event
(degradations) happens to aca glycolic extract at temperatures (T
onset
,
peak, T
endset
) in (33, 71, 99C) with an energy of -3.90 kJ/g, and second at
(117, 135, 149C) and of -1.65 kJ/g energy. Conclusions: A thermal
analysis study on decomposition processes of aa glycolic extract show
your thermal instability and these parameters can be used in the
development and characterization of cosmetics products.

Financial support from CNPq/Brazil and scholarship from
UFRN/Brazil.


184

CODE 175.2
KINETICS STUDIES OF UVB SUNSCREENS: ETHYLHEXYL TRIAZONE
AND ETHYLHEXYL METHOXYCINNAMATE

Rodrigues, R. O.
[1]
; Daher, C. C.
[2]
; Fontes, I. S.
[1]
; Soares, D. S.
[1]
;
Arago, C. F. S.
[3]
; Gomes, A. P. B.
[3]
; Ferrari, M.
[2]

1
2
Faculty of Pharmacy - Potiguar University.
3
Laboratory of Quality Control Faculty of Pharmacy, Federal University
of Rio Grande do Norte.
Corresponding author: rayllanrodrigues@yahoo.com.br

Introduction: Ultraviolet (UV) radiation in sunlight is divided in UVC, UVB
and UVA. This investigation focused on ethylhexyl triazone and ethylhexyl
methoxycinnamate used for protection against UVB radiation. Objectives:
The aim this work was avaliable and compares the kinetics behavior of
sunscreens. Materials and Methods: The TG curves were obtained with a
Shimadzu thermobalance (DTG-60), under a N
2
flow 50mL.min
-1
, at
heating rate 10
o
C.min
-1
, up to temperature of 900C. Sample mass about
10mg were packed in an alumina cell. For the decomposition, the
activation energy (Ea), the order of reaction (n) and the frequency factor
(Z) were obtained from first step of TG curves. The curves obtained were
analyzed for software Shimadzu TA60 TASYS. The kinetic parameters
were calculated with o values of 0.10.9 by utilizing the mathematical
equations of Coats-Redfern (CR), Madhusudanan (MD), Horowitz-Metzger
(HM), and Van-Krevelen (VK). The kinetic data were calculated by four
mathematics models. Results and Discussion: Presents the values of the
order of reaction (n), the Ea and the Z for ethylhexyl triazone and
ethylhexyl methoxycinnamate, with the best linear correlation coefficients
(r): ethylhexyl triazone (n / Ea - kJ.mol
-1
/ Z/s
-1
): CR
(0.55/211.42/6.75x10
13
); MD (0.66/220.57/3.90x10
14
); HM
(0.69/242.02/1.93x10
16
); VK (0.64/241.31/7.24x10
21
); ethylhexyl
methoxycinnamate CR (0.31/91.46/3.80x10
6
); MD (0.07/82.71/4.66x10
5
);
HM (0.29/107.56/1.36x10
8
); VK (0.29/100,55/9.28x10
12
). TGA data allowed
calculating kinetic parameters. The Ea by used methods have shown good
results correlation. Conclusions: Thermal data of the sunscreens supply
important information on evaluation of stability of UVB protection,
becoming an important tool on quality control and development of
cosmetics products.

Financial support from CNPq/Brazil and undergraduate scholarship
from UFRN/Brazil.


185

CODE 176
LECTIN ANTINOCICEPTIVE AND ANTI-INFLAMMATORY ACTIVITY
EXTRACTION, PURIFICATION AND EVALUATION OF Canna limbata
ROSCOE SEEDS.

Tenrio, A. P. M.
2
; Arajo, T. S.
1
; Falco, M. A. P.
2
; Maleski, A. L. A.
2
;
Melo, S. M. S.
2
; Da Silva, F. C.
2
; Silva, D. F.
1
; Correia, I. A. L.
1
; Machado,
S. S.
1
; Martins, J. L.
3
; Cavada, B. S.
4
; Da Rocha, B. A. M
4
; Moreira, M. S.
A.
5
; Mella, E. A. C.
5

1
Instituto de Qumica e Biotecnologia, Universidade Federal de Alagoas
(UFAL);
2
Universidade Federal de Alagoas (UFAL);
3
Instituto de Qumica e Geocincias, Universidade Federal de Pelotas;
4
Departamento de Bioqumica e Biologia Molecular, Universidade Federal
do Cear (UFC);
5
Instituto de Cincias da Sade, Universidade Federal de Alagoas (UFAL)
Corresponding author: priscilla.mag@hotmail.com

Introduction: Lectins are proteins that are capable of specific recognition
of carbohydrate and because of that have several actions, like in
immunology, cell recognition, histology, pharmacology and others.
Objective: Extract e purify lectin of the Canna limbata seed, followed by
the evaluation of the antinociceptive and antiinflammatory activity.
Materials and Methods: Proteics extracts were obtained by seeds milling
e through saline extraction followed by precipitation with ammonium
sulfate, as methodology adaptation described by OITA et al, 2000. Affinity
chromatology using chitin was used in isolation and purification of lectin
molecules. The purification accompaniment was made by hemagglutinating
activity and electrophoresis techniques (sds-page). The experiments were
made using swiss mice (20-30g) approved by Etic Commyte UFAL (n:
015102/2010-51). The nociception and inflamation experiments were:
abdominal writhing, nociception e peritonitis, induced by, respectively,
acetic acid, formalin and carrageenan. The results were analyzed by t test
in prisma(*p<0.05,*<0.01) tutorial. Results and Discussion: Thees
hint of a tetrameric protein (~50kda) consisting of subunit of about 12kda.
In writhing experiment, lectin presented significant inhibitory efect
(10mg/kg=76%, 5mg/kg=70% and 1mg/kg=53%). In formalin experiment,
the lower lectin concentration has the best nociceptive answer in fase 1
(1mg/kg=50%, 5mg/kg=41% and 10mg/kg=30%), and in fase 2
(1mg/kg=75%, 5mg/kg=60% and 10mg/kg=63%) resembling indomethacin.
The peritonitis experiment was able to inhibit in a static way the cell
migration. Conclusion: The Canna limbata seeds lectin structural aspects
resembling with the others recognizers lectins of monocotiledone purified
mannose, besides the properties antiinflammatory and peripheral
antinociceptive activity was observed.

Financial Support from CNP and FAPEAL


186

CODE 177.1
RHEOLOGICAL ANALYSIS IN PRELIMINARY STABILITY STUDIES OF
SUNSCREEN EMULSIONS CONTAINING AAI EXTRACT (Euterpe
oleraceae).

Fontes, I. S.
1
;

Daher, C. C.
2
; Rodrigues, R. O.
1
; Soares, D. S.
1
; Ferrari,
M.
1

Laboratory of Research and Development of Cosmetics Product,
Department of Pharmacy, Federal University of Rio Grande do Norte.
Faculty of Pharmacy - University Potiguar.
Corresponding author: ipojucan_fontes@hotmail.com

Introduction: The rheological properties study can be used to select
different formulations in the development of cosmetic emulsions.
Rheological measurements of the formulations play an important role in the
physical stability. Objective: The aim of this work was to evaluate the
influence of the both emulsifying agent and viscosity modifiers on the
rheological properties in the development of cosmetic emulsions.
Materials and Methods: Emulsions were prepared with different
concentrations of Acrylates/C10-30 Alkyl Acrylates Crosspolymer (0.1% -
P1, 0.2% - P2, 0.3% - P3). The stable emulsion was added of Carbomer
(0.1% - PC1, 0.2% - PC2, 0.3% - PC3). The organoleptic characteristics,
centrifugation test, freezing-thawing cycle (FTC) and thermal stress (TS)
were used to investigative the preliminary stability tests. The physical
preliminary stability was assessed through rheological determinations. The
minimal apparent viscosities and flow index values were evaluated.
Results and Discussion: The P1 and P2 formulations were unstable in
the centrifugation test. The addition of rheological modifier improved
preliminary stability. The PC2 and PC3, formulations have been found to
be preliminary stables. The PC1 formulation showed statistical differences
after TS and FTC tests. The viscosity modifier concentration used
influenced the rheological behavior of the formulations. The rheological
measurements showed that the samples exhibited non-Newtonian
pseudoplastic flow behavior. Conclusions: The P3, PC2 and PC3,
formulations were stables in the standard conditions and should be
conducted to accelerated stability tests. The authors thank Basf, Croda,
ISP and Mapric for the gift of sample materials.

from UFRN/Brazil.


187

CODE 177.2
EVALUATION OF SUNSCREENS THERMAL ANALYSIS BY
THERMOANALYTIC TECHNIQUES

Fontes, I. S.
1
; Daher, C. C.
2
; Rodrigues, R. O.
1
; Soares, D. S.
1
; Arago,
C.F.S.
3
; Gomes, A.P.B.
3
; Ferrari, M.
1

Laboratory of Research and Development of Cosmetic Products, Faculty
of Pharmacy, Federal University of Rio Grande do Norte (UFRN).
Laboratory of Quality Control, Faculty of Pharmacy, Federal University of
Corresponding author: ipojucan_fontes@hotmail.com

Introduction: Exposure to solar radiation has a variety of harmful effects
on human health. Some of these effects include the induction of
photosensitization, immunosuppression, photocarcinogenesis and
photoaging. However, the ultraviolet (UV) dose absorbed by the skin can
be reduced by limiting exposure to the sun through the use of
photoprotective agents such as sunscreens. Consequently a high chemical
stability is an important requirement for an effective UV filter. The thermal
analysis techniques have been used for the characterization of raw
materials and formulation studies. Objective: The aim of this work was to
evaluate the sunscreens thermal stability by thermoanalytic techniques:
thermogravimetry (TG) and differential thermal analysis (DTA). Materials
and Methods: The TG curves were obtained with a Shimadzu
thermobalance, model DTG-60, under a N
2
flow 50 mL.min
-1
, at heating
rate 10
o
C.min
-1
, up to temperature of 900 C. Sample mass about 10 mg
were packed in an alumina cell. The curves obtained were analyzed for
software Shimadzu TA60 TASYS. Results and Discussion: The following
results in descending order of the sunscreens studied and their heats of
reactions by DTA and onset temperature by TG: Bis-ethylhexyloxyphenol
methoxyphenyl triazine (-12, 02 KJ/g; 431,55C); Ethylhexyl triazone (-
12,89 KJ/g; 397,2C); Diethylamino hydroxybenzoyl hexyl benzoate (-19,94
KJ/g; 309C). Conclusions: According to the analyses we found that Bis-
ethylhexyloxyphenol methoxyphenyl triazine decomposed at temperatures
above and is more stable that the other sunscreens.

from UFRN/Brazil.


188

CODE 179
STUDY OF ASSOCIATION BETWEEN OPG POLYMORPHISMS
(- 163A>G; 1181G>C) AND mRNA EXPRESSION IN TYPE 1
DIABETES PATIENTS

Loureiro,M.B.
1
; Ururahy,M.A.G.
1
; Oliveira, Y.M.C.
1
; Souza, K.S.C.
1
;
FREIRE NETO, F. P.
1
; BEZERRA, J. F.
1
; SILVA, H. P. V.
1
; OLIVEIRA,
G.H.M
1
; Hirata,R.D.C.
2
; Arrais,R.F.
3
; Almeida,M.G.
1
; Hirata,M.H.
2
;
Rezende,A.A.
1

1
Department of Clinical and Toxicological Analyses-UFRN/Natal-RN/Brazil;
2
Department of Clinical and Toxicological Analyses-USP/So Paulo-
SP/Brazil;
3
Department of Pediatrics-UFRN/Natal-RN/Brazil

Introduction: Several studies suggest that children and adolescents with
type 1 DM present the risk in long term to suffer a reduction in bone mass,
which could limit the bone growth and increase the probability of
developing osteoporosis and its complications. Objectives: The objective
of present study was to investigate the association between OPG (-
163A>G; 1181G>C) polymorphisms and mRNA expression of OPG in
type 1 diabetic children and adolescents assisted at a pediatric hospital
(HOSPED/UFRN) in Natal-RN/Brazil. Materials and Methods: This study
included 100 type 1 diabetic patients (DM1 group) and 106 normoglycemic
subjects (NG group) aged between 6 and 20 years. Metabolic control was
evaluated by glucose and glycated hemoglobin. Expression of mRNA was
performed by PCR real-time. Polymorphism was determined by allelic
discrimination technique in real-time PCR using TaqMan

pre-designed
SNP assays from Applied Biosystems. Results and Discussion: Glucose
and glycated hemoglobin were significantly increased in diabetic
individuals compared to NG, indicating a poor metabolic control of these
patients. OPG polymorphism (- 163A>G) (NG: TT-71%, CT-28% and CC-
1%; DM1: TT-73%; CT-26% and CC-1,56%) was associated to increased
OPG mRNA expression in type 1 diabetics. No association was found
between OPG polymorphism ( 1181G>C) (NG: CC-38%, CG-47% and
GG-15%; DM1:CC-36%; CG-47% and GG-17%) and OPG mRNA
expression. Conclusions: The increased OPG mRNA expression in the
presence of the OPG (- 163A>G) polymorphism could reflect a possible
protection against bone resorption associated with type 1 diabetes.

Finnantial Support from CNPq, CAPES.


189

CODE 180

THIRD ORDER EXPERIMENTAL APPROACH FOR HUMIDITY DETERMINATION IN
THE LEAVES OF Azadirachta indica A. Juss

Emerenciano, D. P.
1*
, Rodrigues, C. J. F.
1
, Oliveira, W. A.
2
, Maciel M. A. M.
1
, Moura M. F.
V.
1
, LIMA, K. M. G.
1
.

1
Institute of Chemistry, Center of Exact Sciences and Earth - CCET, Federal University of
Rio Grande do Norte, UFRN, Natal / RN, Brazil.
2
Department of Pharmacy, Health Sciences Center - CCS, Federal University of Rio Grande
do Norte, UFRN, Natal / RN, Brazil.
Corresponding author: *deniseemerenciano@yahoo.com.br

Introduction: The medicinal species Azadirachta indica A. Juss (Meliaceae) is
widely cultivated in Asia, Africa and other tropical parts of the world. Scientific
studies developed throughout the world, led to the isolation and structural
elucidation of more than 100 special metabolites, among them terpenoid-types. In
this work a third order experimental approach was used to leaves obtained from A.
indica, aiming both of them punctual and effectiveness results to determine its
humidity. The applied experimental method revealed a succsseful and practical
methodology that could be utilized to any plant species work aiming subsequent
analysis, such as bromatologicals ones. Objective: Development of a third order
experimental approach to determine humidity in the leaves of Azadirachta indica
A. Juss for subsequent bromatologicals analysis. Material and Methods: The
leaves of Azadirachta indica A. Juss were daily collected (in May 2011) during four
days on the UFRN/Natal Campus University. The third order experimental
approach was realized according to ranging parameters such as: vegetal weight
(5 and 10 g), temperature (95 and 105C) and drying period (10 and 15 min.). For
that, the reference method Analytical Standards of the Institute Adolfo Lutz (1985)
was applied. In this method, the humidity of the tested vegetal sample (10 g
utilized in triplicate) was dried at 105C for 24 h. After this period, heating and
cooling (at room temperature in desiccator) were performed at intervals of 1 h,
followed by weighing (until constant weight measurement). In this, present work
the adapted analysis were carried out with plant mass, temperature and drying
period variants on OHAUS-MB200 balance apparatus coupled with infrared.
Results and discussion: Determination humidity analysis following the reference
methodology (Normas Analticas do Instituto Adolfo Lutz, 1985) afforded 68.03%
(+/- 0.02) data. The obtained results by the third order experimental approach
realized according to specifically ranging parameters, monitored via statistical
analysis, afforded variance average around 3.33 with a Tcritical (Tc) value of
5.43%, which was adopted as the reference data for the investigation of those
used parameters). According to the obtained results it was proved that the best
conditions for the modified method involves the following parameters: lower plant
mass (5 g), drying period of 15 min. at the higher temperature (105C). For the
whole daily analysis it was possible to have significant drying results ranging from
63.29% to 66.72%. Specifying, 66.72% (+/- 1.53) for the first day vegetal
collection, 63.29% (+/- 0.26) for the second one, 64.27% (+/- 0.36) and 66.39%
(+/-1.51) for those ones (values are the average of daily triplicates). Conclusions:
Based on the development of a third order experimental approach aiming to
determine humidity in the leaves of Azadirachta indica A. Juss for subsequent
bromatologicals analysis, it was proved that the reference result (68%) and the
obtained data from the approached method (63.29% to 66.72% observed in four
days experimental time) were similar. In that, it was concluded that the modified
method showed significant efficacy with a drastic reduction of analysis time
consumption, since the reference method involves analysis within 10 days time.

Financial support from CNPq / Brazil and CAPES / Brazil
References:


190

CODE 181
HUMAN LEUCOCYTE ANTIGEN REGION AND DIABETIC
NEPHROPATHY IN CHILDREN AND ADOLESCENTS WITH TYPE I
DIABETES OF RIO GRANDE DO NORTE, BRAZIL

Silva, H.P.V.
1
, Ururahy, M.A.G.
1
, Loureiro, M.B.
1
, Souza, K.S.C.
1
, Oliveira,
Y.M.C
.1
,Freire-Neto, F.P.
1
,Bezerra, J.F.
1
, Oliveira, G.H.M.
1
,Hirata, R.D.C.
2
,
de QuateliDoi, S.
3
, Donadi, E.A.
4
, Arrais, R.F.
1
, Almeida, M.G.
1
, Hirata,
M.H.
2
, Rezende, A.A.
1

1
Universidade Federal do Rio Grande do Norte (UFRN) - RN
2
Universidade Estadual de So Paulo (USP) So Paulo - SP
3
Uniformed Services University of the Health Sciences(USU) -Maryland -
USA
4
Universidade Estadual de So Paulo (USP) Ribeiro Preto - SP
Corresponding author: heglayne@yahoo.com.br

Introducion. The human leucocyte antigen (HLA) region has a major
influence on susceptibility to Type I diabetes (TDM1) and is possibly
implicated in susceptibility to diabetic complications as the diabetic
nephropathy. Objective. The purpose of this study was to examine the role
of human leucocyte antigen genes (HLA-DRB1,-DQA1, and -DQB1) in
susceptibility to Type 1 diabetes and diabetic nephropathy. Materials and
Methods. The study was conducted on 29 children and adolescents with
type 1 diabetes under treatment with insulin assisted at the pediatric
hospital (HOSPED/UFRN) in Natal/RN, Brazil, and 71 healthy volunteers
representing control group (CG), with mean 6 to 20 years old in the groups.
DNA was extracted from the peripheral blood mononuclear leukocytes of
patients and controls by use of the Qiagen Kit, Quiagen. The HLA-DRB1
and DQB1 gene alleles were examined by the polymerase chain reaction
(PCR SSO reverse). Results and discussion. The most significant
alleles associated with T1DM were DRB1*03:01 (p>0001; OR=11,79),
DQA1*05:01 (p>0001; OR=9,03) and DQB1*02:01 (p>0001; OR=4,81)). In
addition, the genotypes DRB1*03:01-DRB1*04:05 (p=0,0061; OR=25,24),
DQB1*02:01-DQB1*03:02, (p<0,0001; OR=88,89) and DQA1*03:01-
DQA1*05:01 (p<0,0001; OR=47,67) were positively associated with T1DM.
The haplotypes DRB1*03:01-DQA1*05:01-DQB1*02:01 (p<0,0001;
OR=11,79) and DRB1*04:05-DQA1*03:02-DQB1*03:02 (p=0,0084;
OR=13,30) had a stronger association with susceptibility to T1DM.
7 of 29 T1DM patients had microalbuminuria and presented the following
alleles: DRB1*04:05 (p=0,0095; OR=0,0094) and DQA1*03:02 (p=ns) and
genotypes: DRB1*03:01-DRB1*0405 (p=0,0419; OR=0,070) and
DQA1*03:02-DQA1*05:01 (p=0,0419; OR=0,070) but no significant
association with diabetic nephropathy was seen. Conclusions These
results strengthen the effect of HLA-Class II on susceptibility to T1DM in
children and adolescents of Rio Grande do Norte. No demonstrate the
major role of HLA in the susceptibility to nephropathy..



191

CODE 182
PREPARATION AND CHARACTERIZATION OF INCLUSION
COMPLEXES FORMED OF ESSENTIAL OILS OF Lippia sidoides
Cham AND Lippia origanoides HBK WITH -CYCLODEXTRIN.

Medeiros, M.G.F.
1
, Mouro, L.C.S.
2
, Silva, A.C.
3
, Figueiredo, R.C.B.Q.
3

Moura, T.F.A.L.
2
, Lopes, J.A.D.
4
.

1
Department of Biochemistry and Pharmacology, Federal University of
Piau (UFPI) Teresina Brazil.
2
Laboratory of Drug Development, Department of Pharmacy, Federal
University of Rio Grande do Norte (UFRN) Natal Brazil
3
Department of Microbiology, Research Center Aggeu Magalhes,
FIOCRUZ-PE. Recife Brazil.
4
Department of Chemistry, Federal University of Piau (UFPI) Teresina
Brazil.
Corresponding author: layanymourao@gmail.com

Introduction: Lippia sidoides Cham and Lippia origanoides HBK species
belong to the Verbenaceae family, being widely cultivated in Brazil. They
present biological activities, such as antimicrobial and antiparasitic
properties. The formation of inclusion complexes of CDs with essential oils
increases the stability of the oil and reduces its volatilization which allows
its handling and application. Objectives: The aim of this study was to
prepare and characterize inclusion complexes of essential oils of Lippia
sidoides and Lippia origanoides in -cyclodextrin aiming their application in
the treatment of leishmaniasis. Material and Methods: Inclusion
complexes were obtained by the technique of kneading. About 2.0 g of
essential oil was added to 10 g of a moist paste of |-cyclodextrin and a
solution of ethanol-water (1:2). During 30 minutes, the mixture was mixed
in a mortar until obtaining a solid product which was submitted to drying
(70 C, 16 hours) and sprayed. The samples were characterized by DSC,
SEM and H
1
NMR and the profiles of the oils in natura, the complexed
preparations and the patterns (carvacrol and thymol) were compared.
Results and Discussion: The DSC curves showed disappearance of the
characteristic peak of the essential oils in natura. SEM revealed changes in
the characteristic pen of -CD in the presence of essential oils. The
NMR presented chemical shifts to high field for the carbons 3 and 5 in the
pesence of -CD complexed with the standards and essential oils.
Conclusion: The physical-chemical characterization of preparations
suggests a probable formation of inclusion complexes between the
essen os nd -CD.

Master scholarship from CNPq CAPES/Brazil.


192

CODE 183
ASSOCIATION OF POLYMORPHISMS IN THE IGF-1, TGF-1 AND
LRP5 GENES WITH TYPE 1 DIABETES

Souza, K.S.C.
1
; Ururahy, M. A. G.
1
; Oliveira, Y. M. C.
1
; Loureiro, M. B.
1
;
Silva, H. P. V.
1
; Freire-Neto, F. P.
1
; Bezerra, J. F.
1
; Santos, F. M.
1
; Morais,
L. V. F.; Hirata, R. D. C.
2
; Arrais, R. F.
3
; Almeida, M. G.
1
; Hirata, M. H.
2
;
Rezende, A.A.
1

1
Department of Clinical and Toxicological Analyses-UFRN/Natal-RN/Brazil;
Department of Clinical and Toxicological Analyses-USP/So Paulo-
SP/Brazil;
Department of Pediatrics-UFRN/Natal-RN/Brazil
Corresponding author: Rezende, A.A.:

adrirezende@yahoo.com

Introduction: Type 1 diabetes (T1DM) is a multifactorial disease with an
ey ge of onse, n whch he nsun poducng ce of he pnces e
destroyed because of autoimmunity. Polymorphisms in genes, like IGF-1,
TGF-1 nd LRP5, coud be ssoced o ype 1 Dbees (T1DM) nd s
complications. Objectives: The objective of present study was to
investigate the association between IGF-1 (-1245G>A), TGF-1 (-509C>T)
and LRP5 (126188 C>T) polymorphisms in the development T1DM in
patients assisted at a pediatric hospital (HOSPED/UFRN) in Natal-
RN/Brazil. Materials and Methods: This study included 134 type 1
diabetic patients (T1DM group) and 181 normoglycemic subjects (NG
group) aged between 6 and 20 years. Metabolic control was evaluated by
glucose and glycated hemoglobin. Polymorphism was determined by allelic
discrimination technique in real-time PCR using TaqMan

pre-designed
SNP assays from Applied Biosystems. Results and Discussion: Glucose
and glycated hemoglobin were significantly increased in diabetic
individuals compared to NG, indicating a poor metabolic control of these
patients. LRP5 (126188 C>T) polymorphism was associated to T1DM (NG:
CC-83.4%, CT-16% and TT-0.6%; T1DM: CC-70.7%, CT-27.1% and TT-
2.2% - p=0.020). No association was found between IGF-1 (-1245G>A)
(NG: GG-45.52%, GA-47.59% and AA-6,90%; T1DM:GG-53.66%; GA-
39.02% and AA-7.32% - p=0.626) and TGF-1 (-509C>T) (NG: GG-
46.90%, GA-40% and AA-13.10%; T1DM:GG-41.46%; GA-45.12% and
AA-13.41% - p=0.998) polymorphisms and T1DM. Conclusions: These
results revealed an association between LRP5 (126188 C>T)
polymorphism on susceptibility to T1DM in children and adolescents,
showing their possible relationship in the pathophysiology of the disease.

Financial Support from CNPq/Brazil.


193

CODE 184
VALIDATION OF UV SPECTROPHOTOMETRIC ANALYTICAL
METHOD FOR THE DETERMINATION OF PRAZIQUANTEL IN
INCLUSION COMPLEXES WITH -CYCLODEXTRIN IN PRESENCE OF
HYDROXYPROPYLMETHYLCELLULOSE.

Mouro, L.C.S.
1
, Batista, D.R.M.R.
1
, Morais, W.A.
1
, Moura, T.F.A.L.
1

1
Laboratory of Drug Development, Department of Pharmacy, Federal

Corresponding author: layanymourao@gmail.com

Introduction: Praziquantel (PZQ) is the drug of choice for the treatment of
schistosomiasis, however, is classified as Class II in the Biopharmaceutics
Classification System due to its low solubility. The use of cyclodextrins is a
useful tool for increasing the solubility and bioavailability of class II drugs.
Objective: The aim of this study was to develop and validate a quantitative
UV spectrophotometric method for determination of praziquantel in
inclusion complexes with - cyclodextrin and
hydroxypropylmethylcellulose. Material and Methods: The validation
parameters were assessed according to The International Conference on
Harmonization (ICH) and American Pharmacopoeia guidelines. To
numerically evaluate the quality of fit of the models, we used analysis of
variance (one-tailed test, p <0.05). The means were evaluated by Student's
t test (two-tailed test, p <0.05). Results and Discussion: The linearity
range was 120-1020 g.mL
-1
, regression equation: absorbance = 0.00111
x PZQ concentration (g.mL
-1
) + 0.00508 and r = 0.9998. For the other
parameters were analyzed triplicate concentrations of 120, 570 and 1020
g.mL
-1
. The repeatability of the method was 2.66, 1.41 and 0.56% and
intermediate precision was obtained: 3.41, 1.70 and 0.30%. The method
proved to be selective, robust against changes in temperature, the solvent
and the wavelength used. The accuracy indicated that the difference
among mean percentages of recovery were no statistically significant (p
<0.05). The detection and quantification limits of praziquantel were found
to be 0.22 and 0.667 g.mL
-1
respectively. Conclusion: The proposed
method is accurate, precise and reproducible for estimation of praziquantel
as raw material and in pharmaceuticals dosage forms such as inclusion
complexes.

Financial support from Master scholarship from CNPq
CAPES/Brazil.


194

CODE 185
EVALUATION OF COMPONENTS OF METABOLIC SYNDROME IN
ADOLESCENTS WHO ARE OVERWEIGHT ACCORDING TO THE
NATIONAL CHOLESTEROL EDUCATION PROGRAM - ADULT
TREATMENT PANEL III

Melo, M.M.A.; Ramos, A.T.R.; Medeiros, C.C.M.; Farias, A.A.L; Flix,
B.F.

Department of Pharmacy, State University of Paraba (UEPB)
Center for Studies and Research and Epidemiology (NEPE), State
University of Paraba (UEPB)
Corresponding author: menillamelo@yahoo.com.br

Introduction: The Metabolic Syndrome (MS) features by a set of metabolic
disorders that if no treated and/or prevented can be initiate related
diseases to cardiovascular risk (CVR). Objective: Evaluate the
components of MS among overweight adolescents in order to raise
awareness of the current world problems. Materials and Methods: Cross
sectional study of 146 overweight adolescents conducted between April
2009 and March 2010 at the Center for Childhood Obesity, Campina
Grande-PB. To obtain the MS we used NCEP-ATPIII values. Has been
physical examination, biochemical evaluation and body composition. In the
proportions associated analysis of the diagnostic criteria with the patient
gender, we used the Qui-square, has been adopted a significance level
less than or equal to 5%. Results and Discussion: 81.5% of the sample
wee obese (pecene 95) nd 18.5% ovewegh (pecene 85 95).
The MS has been detected in 39 (26.7%) of patients, most of obese girls.
Low HDL-c was the most common component, in 127(87%) of the patients.
There wasnt significant difference between the genders (p>0.05) for the
values of triglycerides, glucose and blood pressure. However, the results of
abdominal circumference and HDL-c showed discrepancies between the
normal and abnormal proportion. For the first, 16% of boys and 55.2% of
girls presented abnormal values (x
2
=20.77; p<0.001). For the HDL-c 92.7%
of girls and 76% of boys(x
2
=8.10; p=0.004) presented changes.
Conclusion: The obesity degree influenced in the appearance of changes
that make up the MS, suggesting more predisposition of CVR in overweight
adolescents.


195

CODE 186
ENERGETIC ASPECTS OF CHEMICAL INTERACTION BETWEEN THE
VIOMICYN ANTIBIOTIC AND BACTERIAL RIBOSOME

Vianna, J. F; Fulco, U. L; Albuquerque, E. L; Oliveira, J. I. N; Neto, J. X.
L

1
Norte, 59072-970, Natal, RN, Brazil.
Corresponding author: viannajf@gmail.com

Introduction: The Mycobacterium tuberculosis is the causative agent of
tuberculosis, a disease that provoked 2 million deaths in 2007, and
estimates of 9 milion new recurrences. This bacterium has become
resistant to antibiotics commonly used, and thus triggered a major public
health problem. The Viomycin is a new anti-tuberculosis aminoglycoside
that inhibits protein synthesis by preventing the translocation step in
translation, due to stabilization of tRNA in site A. Objective: In this study,
we measure the energy of interaction of this drug on bacterial ribosome in
order to understand its pharmacological action. Materials and Methods:
From the viomycin-70S ribosome crystal, the energy calculations were
carried out by DFT-MFCC method. Calculations were made for each
nucleotide present at the binding site, considering a radius of 20 from the
center of mass of the ligand. Results and Discussion Thus, identify these
C1491 (-12,954Kcal/mol), C518 (-9,762Kcal/mol), G1494 (-8,38Kcal/mol),
A1408 (-8,085Kcal/mol) and C1409 (-4,886Kcal/mol) are residues that
interact more strongly with the drug. The calculated interaction energies
povde nsgh on specc dug bndng nd e vey usefu o ou
understanding of drug binding to ribosomes.



196

CODE 187
VALIDATION OF A HPLC METHOD FOR THE QUANTIFICATION OF
DIBUCAINE IN SOLUTION AND ENCAPSULATED IN SOLID LIPID
NANOPARTICLES OR NANOSTRUCTURED LIPID CARRIERS

Barbosa, R. M
1
, Marcato, P.
2
, Franz-Montan, M
1
, Bella, T.
1
, de Paula, E.
1
.

1
Department of Biochemistry, State University of Campinas (UNICAMP).
C.P. 6109, CEP 13083970, Campinas, SP, Brazil.
Chemistry Institute, Biological Chemistry Laboratory, State University of
Campinas (UNICAMP), C.P. 6154, Campinas, CEP 13083-970, S.P.,
Brazil.
Corresponding author: m.g.barbosafernandes@gmail.com

Introduction: Pain is the focus of many studies in the scientific community.
Local anesthetics (LA) relieve pain by reversibly blocking the process of
excitation-conduction in peripheral nerves. Dibucaine (DBC), used mainly
in topical formulations, is one of the most potent long-acting local
anesthetics, but is also one of the more toxic. Recently, Solid Lipid
Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC) have
attracted attention as a promising carrier for drug delivery. Objective: The
purpose of the present study was to develop and to validate an analytical
methodology for quantifying DBC into SLN and NLC formulations, prepared
through high pressure with different lipid matrixes (myristyl myristate, MM
or cetyl palmitate, CP) by HPLC. Materials and Methods: The
quantification was done using the reversed-phase C18 column, a mobile
phase consisting of a mixture of acetonitrile and triethylamine phosphate
buffer (pH 2.3, 0.04M, v/v) (55:45 v/v), a flow rate of 1.0 mL/min and UV-
Vis detector at 247 nm. Results and Discussion: The results show that
the analytical methodology is accurate, reproducible and robust; the
method was linear in the concentration range 1.5
correlation coefficient (r =0.999). The limit of quantification and detection
dibucaine was over 78% for SLN and NLC samples. Conclusions: The
developed method was applied in the analysis of DBC encapsulated in
SLN and NCL yielding good results. It will be used in future studies with
different nanoparticle formulations.

Financial support from Fapesp and Capes.


197

CODE 188.1
EVALUATION OF ANTIBACTERIAL POTENTIAL OF THE LEAVES OF
Senna occidentalis (L.) LINK

Feitosa, I. L. F.; Machado, S. E. F.
1
; Batista, R. S. A
1
; Felismino, D. C.;
Dantas, I. C.

Departamento de Farmcia, Universidade Estadual da Paraba (UEPB).
Departamento de Biologia, Universidade Estadual da Paraba (UEPB).
Corresponding author: layany_feitosa6@hotmail.com

Introduction: The use of natural resources as an auxiliary mechanism in
the treatment of different diseases has risen, with an emphasis in recent
times. Objective: This study aimed investigate the antimicrobial activity of
Senna occidentalis (L.) Link leaves in the form of fluid extract and lushness
in the face of standardized strains of Staphylococcus aureus,
Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae.
Materials and Methods: The evaluation of the antimicrobial activities of
extract and lushness were performed by disk diffusion technique on solid
space. In the screening, the disks were sterile filter paper, which were
impregnated with 20L fluid extract or lushness at concentrations of 100%.
As a positive control, antibiotics Gentamicin disks were used for the strain
of S. aureus and Aztreonam disks for the other strains; as a negative
control, paper disks of sterile filter, impregnated in water-alcohol solution at
70GL were used. The assays were performed in triplicate and incubated
at 37C/48 hours. Halos of growth inhibition equal or bigger than 8 mm in
diameter would prove the antibacterial effect of plant species. Results and
Discussion: The results showed that the leaves of Senna occidentalis
showed no antimicrobial activity against the strains tested, as opposed to
literature. Conclusions: We suggest using the fractionated extraction and
other methods of incubation in order to prove the effectiveness of this
plant.


198

CODE 188.2
EVALUATE OF THE ANTIFUNGAL ACTIVITY OF Senna occidentalis
(L.) LINK.S LEAVES FORWARD TO STRAINS OF Candida spp

Feitosa, I. L. F.; Machado, S. E. F.
1
; Batista, R. S. A
1
; Felismino, D. C.;
Dantas, I. C.

Departamento de Biologia, Universidade Estadual da Paraba (UEPB)
Corresponding author: layany_feitosa6@hotmail.com

Introduction: Recently the resistance to antimicrobial drugs prove its
lower efficiency, due to increased microbial resistance, overuse of these.
The research of new substances were intensified because of this fact. The
use of herbal extracts and forms of natural remedies has gained
importance in current therapy. The Senna occidentalis (Mangerioba) has
therapeutic effects as anti-inflammatory and antimicrobial. Objective: The
sudy evued he nfung of he Senn occdenss eves n
concentrated forms of fluid extract and lushness in the face of standardized
strains of Candida tropicalis, C. guillermondi, C. krusei, C. parapsilosis and
C. albicans. Materials and Methods: The antifungal activity of the extract
and the lushness were evaluated separately by the disk diffusion technique
on solid space. Paper discs impregnated were used with 20L sterile filter
and extract lushness. As a positive control, we used the Nystatin disks, and
the negative control were made using discs with water-alcohol solution at
70GL. The tests were made in triplicate and incubated at 37C/48 hours.
The halos of growth inhibition equal or bigger than 10 mm diameter
confirming the effect of antifungal Mangerioba. Results and Discussion:
There was no antimicrobial activity of this plant, being contrary to the
literature. The solvent (ethanol 70L) may not have been ideal to extract
the active ingredients in fungicidal effect against the strains. Conclusions:
It need to repeat the study using new methods such as fractionated
extraction and other alternative objective evidence of incubation activity.


199

CODE 189
CHITOSAN NANOPARTICLES FOR PROTEIN DELIVERY OBTAINED
BY IONIC GELATION

Soares, K. S. R
1
; Fonseca, J. L. C
2
; Santos, K. S. R
1
; Silva-Jnior, A. A
1
;
Fernandes-Pedrosa, M.F.
1

Graduate Program on Pharmaceutical Sciences, Federal University of Rio
Grande do Norte, UFRN, Natal-RN, Brazil.
Chemistry Departament, UFRN, Natal, RN.

Introduction: Anti-sera against the scorpions are routinely produced by
various institutions and despite its effectiveness depend on the quality and
speed with which treatment is started. Several studies have been
developed in the research for appropriate technologies to encapsulate and
release recombinant proteins capable of inducing antibody production.
Much attention has been given to chitosan to be a bioactive natural, non-
toxic, biocompatible and biodegradable. It has been extensively
investigated for formulating a nanocarrier for therapeutic macrosolutes,
particularly genes and protein molecules. Objective: This study aims to
obtaining nanoparticles based on chitosan by ionic gelation, to delivery
peptides / proteins of the venom of the scorpion T. serrulatus, able to
provide a new system of immunization in animals, in order to obtain a new
serum polyclonal anti T. serrulatus venom. Material and Methods:
Chitosan nanoparticles were prepared by ionic gelation methodology using
the crosslinker agent polyanion tripolyphosphate (TPP). The evaluation of
the size distribution of particles was performed using the Zeta Sizer.

Different parameters such as volume and concentration of reagents, pH
and zeta potential of the solutions were evaluated with aim to optimize the
methdodlogy. Results and Discussion: Chitosan nanoparticles were
successfully obtained using established parameters for ionic gelation
method. The best conditions optimized lead to obtaining nanoparticles with
size about 187,13+-5,11. Conclusions: The methodology was optimized
successfully. Chitosan nanoparticles prepared by ionic gelation technique
presented a very suitable particle size distribution. A direct correlation
among the parameters of the reagents concentration and zeta potential
was identified cleary.



200

CODE 190
CLINICAL-EPIDEMIOLOGICAL PROFILE OF PATIENTS WITH BREAST
CANCER IN TO THE COMBINATION CANCER CHEMOTHERAPY 5-
FLUOROURACIL, EPIRUBICIN AND CYCLOPHOSPHAMIDE

Falco, A.H.P.B.M.
1
, Cunha, G. H.,Leite, A.L.A.S.
,
*

Costa, M.A.R
2
,Jamacaru, F.V.F.
3;
Moraes,M.E.A.

Unidade de Farmacologia Clnica, Departamento de Fisiologia e
Farmacologia, Universidade Federal do Cear (UFC)
Acadmica de Farmcia, Faculdade de Farmcia, Universidade Federal
do Cear (UFC)
Unidade de Farmacologia Clnica, Departamento de Fisiologia e
Farmacologia, Universidade Federal do Cear (UFC)
Corresponding author: melissacosta14@gmail.com

Introduction: Breast cancer is one of the most frequent among women.
Risk factors include personal and family history of breast cancer, early
onset of menstruation and ending late, no pregnancies or pregnancies late,
among others. Objective: To identify the profile of patients with breast
cancer undergoing chemotherapy combination with 5-fluorouracil,
epirubicin and cyclophosphamide (FEC) in a clinic in Teresina-PI.
Materials and Methods: The research was through medical records. The
project was submitted to the Ethics Committee of the Federal University of
Cear, and approved on 14/05/2009 under protocol No. 127/09 COMEP.
Results and Discussion: Only 23 patients underwent chemotherapy with
FEC regimen. The age ranged from 39 to 76 years (mean: 54.3 years, SD:
9.29). With regard to reproductive life, the average age of menarche was
13.7 years and 47.2 years of menopause. The average number of
pregnancies and deliveries were 2.2 and 1.8, respectively. Regarding
marital status, 12 patients were married and 11 unmarried (single,
separated and widowed). Regarding lifestyle, 8.7% were smokers and
consumed alcoholic beverages. The study population, 10 women (43.4%)
reported family history of cancer. In addition, 21.7% said they breastfed
and 13.0% had a history of oral contraceptive use as a con traceptive
method. Conclusions: The study showed that the sample had some risk
factors which increase the possibility of a woman developing breast
cancer.


201

CODE 191.1
ATIMICROBIAL ACTIVITY OF BIOACTIVITY METABOLITES ISOLATED
FROM Streptomyces spp. FRONT OF PATOGENIC FUNGI YEAST

OLIVEIRA, G. N.
1
; OLIVEIRA, T. L.
2
; LIMA, E. O.
2
; FREITAS, F. I. S.
2

1
Departamento de Cincias Farmacuticas, Centro de Cincias da Sade
(CCS), Universidade Federal da Paraba (UFPB).
2

Introduction: Due to the prevalence of fungal infections with strains
multiresistant to antibiotics used in therapy, we seek to further treatment as
an alternative to the bioactive metabolites from natural sources. Among the
Actinobacteria is the genus Streptomyces are bacteria isolated from soils
with high potential for production of antibiotics. Objective: The objective of
the search for native strains of Streptomyces spp order to characterize the
antimicrobial activity against pathogenic fungi from yeast extracts of
Streptomyces spp. Materials and Methods: 03 strains were isolated from
soil of the city of Joao Pessoa-Pb, obtained by suspension of the soil
sample and streaked into a Petri dish. The identification was performed by
macro-morphological, micromorphological, and physiological tests. The
antimicrobial screening was determined by the agar block technique, and
the dry ethanolic extract was prepared from the species with the best
results in screening. The antagonism was determined using the disk
diffusion testing on solid medium plates and microdilution Elisa. Results
and Discussion: The inhibition halos obtained from the extract JPA and
JPA-S1-S2 showed an antagonistic effect with values between 10-20 mm
above the halos of inhibition promoted by the drug control. Conclusions:
The results of the microdilution minimum inhibitory concentrations were
significant between 10 mg and 5 mg. The results characterize an
antimicrobial potential of the extract against the Candida spp species of
clinical origin. It is appropriate and worthwhile to conduct further sampling
of soil, revealing study of Streptomyces spp. biotechnology as an important
source for obtaining new bioactive compounds.

Financial support CNPq/Brazil.


202

CODE 191.2
ACUTE TOXICITY OF CARVACROL

Lima, I. O; Oliveira, G. N
2
; Nbrega, F. M.
2
; Dias, G. E. N.
3
; Oliveira;
Thompson L.
4
; Diniz, M. F. F. M.
4
.

1
Programa de ps-graduao em Produtos Naturais e Sintticos Biativos /
Centro de Cincias da Sade (CCS) / Universidade Federal da Paraba
(UFPB)
2
3
Programa de ps-graduao em Desenvolvimento e Inovao
Tecnolgica em Medicamentos / UFPB/ Universidade Federal do Rio
Grande do Norte (UFRN) / Universidade Federal do Cear (UFC) /
Universidade Federal Rural de Pernambuco (UFRPE)
4

Introduction: Carvacrol is a monoterpene with antibacterial, antifungal,
antiinflamantory, antioxidant actions. The acute toxicity of mix of carvacrol
(93%) and thymol (7%) was studied in mice at 225mg/kg (v.o.), but there is
not registers about its effect on biochemical and hematological
paramenters (Fitoterapia, v. 75, p.801-3, 2004). Objective: The aim of this
work was to evaluate the toxicity of carvacrol (98%) in mice with a simple
dose. Material and methods: Mice of either sex (six females and males,
weight of 25-35g) received 250 mg/kg of carvacrol (v.o.) and another group
received the vehicle (control). The animals were observed during 14 days
and the last day, the mice were euthanized. The biochemical and
hematological paramenters was evaluated, the weight of organs and any
alterations were observed (Psicofarmacologia, p.131-7, 2006; RE
90/2004). Results and Discussion: The carvacrol caused the lost of
corneal reflex at 30 minutes after its administration. This phytoconstituent
decreased the water consumption in male and female mice. The
monoterpene increased the aspartate amino transferase (AST) in male
mice. There were not died mice. Conclusions: These results showed the
low toxicity of carvacrol, however it is necessary more studies with
phytoconstituent.

CAPES/Brazil


203

CODE 192
EVALUATION OF THE TOXICITY OF Pradosia huberi
HYDROALCOHOLIC EXTRACT ON THE HYPOPHYSIS-GONADAL
AXIS OF PREGNANT FEMALE RATS

Rocha, A. O. B
1
; Souza, L. Q. S.
1
; Sousa, T. M
1
; Figueiredo, R. R.
1
;
Potiguara, R. K. S.
1
; Pita, J. C. R
1
; Santos, E. C. S.
1
; Medeiros, A. A.
N.
2
;Diniz, M. F. F. M.
3
; S, R. C. S.
4

1.
Program of Post graduation in Natural and Syntactic Bioactive Products,
Center of Health Sciences LTF/UFPB, Brazil
2 .
Institute of Technological Research of Amap - IEPA, Brazil
3.
Department of Pharmaceutical Sciences, Center of Health Sciences -
UFPB, Brazil
4.
Department of Physiology and Pathology, Center of Health Sciences -
UFPB, Brazil
Corresponding author: lilianeufpb@gmail.com

Introduction: Pradosia huberi Ducke (Sapotaceae) is a medicinal plant
commonly found in the Amazonian forest and popularly used to treat
gastritis and gastric ulcer. The presence of saponins, flavonoids, steroids
and triterpenes has been identified in the stem bark of this species.
Objectives: In this study, the toxicological effect of the hydroalcoholic
extract (HAE) obtained from P. huberi barks was investigated on the
organs of the reproductive axis (hypophysis, ovaries and uterus) of
pregnant female rats. Materials and Methods: Female Wistar rats were
distributed into four groups (containing 15 or 16 animals each), in which
three of them were treated orally with HAE of P. huberi (T1 = 1,22; T2 =
6,1; T3 = 30,5 mg/kg) and one received distilled water, once daily, from
the first to the seventh day of gestation (preimplantation phase). The
animals were killed by an overdose of ketamine on the 8
th
day of
gestation. After laparatomy, the following organs were removed and
weighed (absolute and relative weight): hypophysis, ovaries and uterus.
Results and Discussion: The relative weight of the hypophysis of the
treated groups (T1 = 0,049 0,002*; T2 = 0,054 0,003* and T3 = 0,051
0,003*) increased significantly, when compared to the group control (C
=0,040 0,001) on the 8
th
day on gestation. Conclusions: The results
indicate a possible toxic effect of P. huberi HAE on the hypophysis, which
could interfere with the normal functioning of the hypophysis-gonadal
axis.



204

CODE 193.2
DRUG PROFILE OF ELDERLY DIABETES AND DYSLIPIDEMIA
ATTENDED BY PHARMACEUTICAL CARE PROGRAM IN A
COMMUNITY PHARMACY.

Brando, D. O.
1
; Palmeira, A. C.
2
, Fernandes, F. H. A.
1
; Clementino, E.
L. C. ; Simes, M. O. S.
1,2,3
, Medeiros, A. C. D.
1,3*

1
Departamento de Farmcia, Universidade Estadual da Paraba , Campina
Grande PB, Brasil,
2
Programa de Ps-graduao em Sade Pblica, Universidade Estadual
da Paraba, Campina Grande PB
3
Estadual da Paraba, Campina Grande PB
* Corresponding author: deysiane_farmacia@hotmail.com

Introduction: The knowledge of the profile of drug use of the geriatric
population is fundamental to the design of strategies for rational
prescription of drugs for the elderly. Research of population-based can
subsidize the professional development of health actions for improving
drug therapy in these patients. Objectives: To describe the
pharmacological profile of a population of diabetic elderly and dyslipidemic
served in a Community Pharmacy in Campina Grande-PB. Materials and
Methods: This was a cross-sectional study where 450 patients, consumers
of drugs for chronic diseases in a Community Pharmacy answered a
questionnaire based on the methodology Dder and in sheet reporting of
adverse reactions ANVISA. It was used Epi Info (version 6.0) for statistical
analysis. Results and Discussion: Of the accompanieds patients, 12.0%
used hypoglycemic and 8.0% used statins. Regarding to the antidiabetics,
68.0% used metformin (biguanide) and 38.0% used glibenclamide
(Sulfoniluria). Of the drugs used for dyslipidemia, the only available at the
pharmacy, the object of this research was simvastatin. The average
number of medications used was 4.0 drugs per patient. With relation to the
treatments adherence, it found that most patients had a regular
membership. It was observed that of the interviewed, 27.0% made use of
self-medication. It also observed that the average of RNMs found in
patients was 2.8 per patient. Conclusions: There was a high
polypharmacy in patients interviewed, as well as possibles drug
interactions in prescriptions, highlighting the importance of using some
monitoring tools in care pharmaceutical that make possible a constant
evaluation of these patients.

CAPES/Brazil.


205

CODE 194
EVALUATION OF HEMATOPOIESIS IN MALNOURISHED MOUSE
MODEL

Pereira, PRS
1
; Silva, BCO
1
, Avelino, CUR
1
; Borelli, P
2
; Schimieguel, DM
1

1
Laboratrio de Hematologia Clnica; Departamento de Anlises Clnicas e
Toxicolgicas, Centro de Cincias da Sade, Universidade Federal do Rio
grande do Norte (UFRN).
2
Faculdade de Cincias Farmacuticas, Departamento de Anlises
Clnicas e Toxicolgicas, Universidade de So Paulo (USP).
Corresponding author: pauloricardo306@yahoo.com.br

Introduction: Protein-energy malnutrition (PEM) is defined by WHO as a
set of pathological conditions that result from a lower intake of protein and
calories. The impact of PEM on hematopoiesis is associated with
decreased cell production, leukopenia, anemia and immune system
abnormalities. Objective: Evaluate parameters related to maintenance of
hematopoiesis in a murine model of malnutrition. Materials and Methods:
We studied 26 animals, 7 adult males and 19 females divided into two
groups: malnourished M (n = 12) which was fed with low protein diet and
control-C (n = 14), fed with normal diets. Samples of peripheral blood (SP)
were used for assessment of blood counts and serum total protein and
albumin. Samples of bone marrow (BM) were obtained by washing the
cavity of femurs and tibias and used in clonogenic assays for enumeration
of G-CFU and GM- CFU stimulated with growth factors and cytokines, and
cell proliferation in 24h, 48h and 72h. Results and Discussion: Weight
loss was observed significantly in group M compared to group C
(p=0,0002). Hematological parameters showed lower hematocrit in group
D (p=0,0025), hemoglobin (p=0,0086) and erythrocytes (p=0,0405).
Clonogenic assays showed a significant decrease in scores of G-CFU
(p=0,0386) of group D. Cell proliferation assays showed no differences
between two groups in the three periods studied. Conclusion:
Malnourished animals showed parameters that characterize malnutrition
process, which corroborates current literature showed that hematopoietic
progenitor cells was lower quantitatively and functionally due to delayed
maturation of colony forming unit in vitro, affecting balance of
hematopoiesis .


206

CODE 195
REPORTING OF DRUGS INDICATORS OF ADVERSE DRUG
REACTIONS: STUDY IN A UNIVERSITY HOSPITAL

Oliveira, R. A.
1
; Pinto, C. O.
1
; Medeiros, J. W. T.
1
; Irineu, M. M.
1
; Barbosa,
M. M. S.
2
; Brito, A. E.
2

1
Academic of Pharmacy, University Hospital Onofre Lopes (HUOL),
Federal University of Rio Grande do Norte (UFRN)

2
Pharmacist, University Hospital Onofre Lopes (HUOL), Federal University
of Rio Grande do Norte (UFRN)
Corresponding author: raquelaraujo_@hotmail.com

Introduction: World Health Organization (WHO) defines adverse drug
reaction (ADR) as "a response to a drug that is noxious and unintended
and occurs at doses normally used in man for the prophylaxis, diagnosis or
hepy of dsese. Abou 5% of hosp dmssons e due o ADR nd
at least 15% of inpatients have adverse reactions. One can check if there
are suspected ADRs by observing the prescription drugs used to treat
them. Objective: Evaluate the importance of reporting of drugs indicators
of ADRs by pharmacists. Materials and Methods: Data was obtained from
the reporting of suspected ADRs from patients as well as the forms used
for the investigation of them at University Hospital Onofre Lopes, a
teaching hospital, tertiary, with 189 beds, in the period from October 2010
to July 2011. The ADRs were classified according to the type of notification
(voluntary, active search and notification of drugs indicators) and according
to the causality and severity. Results and Discussion: 45 adverse drug
reactions were reported, of which 15 (33.4%) were reports of drugs
indicators, 18 (40%) volunteers and 12 (26.6%) by active search. As for
causality, 34 (75.5%) were probable, 9 (20%) possible, one (2.25%) was
defined and one (2.25%) was doubtful. In severity, 31 (68.8%) were
moderate, 9 (20%) mild, 3 (6.7%) severe and 2 (4.5%) lethal. Conclusion:
Through the observation of drug indicators of ADRs, one can find a greater
number of adverse reactions, which could often be imperceptible. Thus,
there was a significant contribution to the increase in ADRs reporting.


207

CODE 196
STUDY OF TAQI/TGF POLYMORPHISM IN NON-SYNDROMIC CLEFT
LIP AND/OR PALATE.

Oliveira, G. H. M.
1
, Cardoso, M. L.
1
; Bezerra, J .F.
1
; Soares, C. D.
1
; Silva,
H. P. V.
1
; Silbiger, V. N.
1
; Luchessi, A. D.
1
; Oliveira, A. M. L.
1
; Barbosa, A.
C. S.
1
; Fajardo, C. M.
2
; Hirata, R. D. C.
2
;

Almeida, M. G.
1
; Rezende, A.
A.
1
; Hirata, M. H.
2

1
Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil.
2
University of Sao Paulo (USP), So Paulo, SP, Brazil.

Introduction: Nonsyndromic cleft lip patients with or without cleft palate
(NSCL/P) are congenitally developmental disorders resulting from defects
in growth and patterning of the facial primordial. TGF is one of candidates
genes suggested to be associated with NSCL/P. Objectives: The aim of
this study was to standardize the methodology for High Resolution Melting
Real Time PCR for search the polymorphism TaqI/TGF and evaluate
allelic distribution in order to assess the association of alleles with
development of oral clefts. Materials and Methods: 140 patients NSCL/P
participated in this study. Blood samples were collected and genomic DNA
was extracted and screened by HRM - Real Time PCR (Applied
Biosystems) and confirmed by sequencing the ABI 3730 DNA Analyser
(Applied Biosystems), USP/SP. Results and Discussion: Most of the
patients studied showed a high frequency of C1C1 genotype independent
of the type of cleft. 65 individuals with Cleft Lip and Palate (CLP) (79.2%),
29 individuals with Cleft Palate (CP) (87%) and 23 individuals (92%) with
Cleft Lip (CL) revealed this genotype C1C1. The heterozygote genotype
C1C2 was present in 13 CLP individuals (15.5%), 4 CP individuals (5.3%)
and only 2 CL individuals (8%). The C2C2 genotype was less frequent and
only 4 patients with CLP had this genotype. Analyzing the alleles C1C1,
C1C2 and C2C2 no significant difference statistically significant (P=0,375)
associated with the type of cleft. Conclusions: The results identified the
pesence of poymophsm TqI n TGF by HRM-Real Time in our patients
although there is no association with the development of oral clefts. This
study need be deepened for a better understanding of the genetic factors
associated to NSCL/P in the population of Rio Grande do Norte.

Financial support from FAPESP/Brazil and CAPES/Brazil


208

CODE 197
Phyllantus niruri L. REDUCE DAMAGE IN THE MUCOSAL IN MODEL
OF COLITIS

Melo, M. N.
1
; Soares, L. A. L.
2
; Jnior, R. F. A.
3
; Ferreira, A. A. A
4
;
Teixeira, H. G. A.
4
; Camillo, C. S.
3
;

Guerra, G. C. B.
4

1
Graduate Program on Pharmaceutical Sciences, Federal University of
Pernambuco (UFPE);
2
University of Pernambuco (UFPE);
3
Laboratory of Histology, Department of Morphology, Federal University of
Rio Grande do Norte (UFRN);
4
Laboratory of Pharmacology, Department of Biophysics and
Pharmacology, Federal University of Rio Grande do Norte (UFRN);
Corresponding author: gerlaneguerra@hotmail.com

Introduction: The Inflammatory Bowel Disease (IBD) are chronic
diseases, which manifest as Crohn's Disease and Ulcerative Colitis, and
are characterized by alternating periods of intense intestinal inflammation
and periods of remission. The etiology remains unknown, although it is
believed that an alteration in the intestinal immune system contributes to
the inflammation that occurs. As in other inflammatory processes,
inflammatory bowel disease is characterized by an up-regulation in the
synthesis and release of different pro-inflammatory mediators, including
reactive oxygen and nitrogen metabolites, eicosanoids, platelet-activanting
factor and cytokines. The classical treatments for IBD are based on drugs
that act by inhibiting the production of prostaglandins and leukotrienes,
such as aminosalicylates, and the use of immunosuppressive agents.
Objective: To assess the activity of standardized dry extract of Phyllanthus
niruri (SDEPn) in model of acute ulcerative colitis. Materials and Methods:
Rats of the Wistar strain were used, which were divided into five groups:
colitis (vehicle), group I (SDEPn 100mg/kg), group II (SDEPn 200 mg / kg),
sulfasalazine group (500mg/kg) and non-colitis group (vehicle). Method
was used to induce acute colitis with acetic acid 10%. The results were
evaluated on macroscopic and histopathological findings. Results and
Discussion: The rate of macroscopic damage and histological analysis
have shown that preventive treatment with SDEPn significantly reduced the
intensity of intestinal inflammation caused by acetic acid compared with the
colitis group. Conclusions: The SDEPn exerts beneficial effects because
reduce damage in the intestinal mucosal in model of acute colitis.


209

CODE 198
COMPATIBILITY BETWEEN CAPTOPRIL AND EXCIPIENTS THROUGH
THERMAL ANALYSIS

Goes, J. S.
1
; Freire, F. D.
1
; Moura, T. F. A. L.
1
; Raffin, F.
1

1
Laboratrio de Desenvolvimento de Medicamentos, Departamento de
Farmcia, Universidade Federal do Rio Grande do Norte (UFRN).
Corresponding author: janainagoesfarm@yahoo.com.br

Introduction: Captopril was the first orally active potent and specific
inhibitor of the enzyme (ACE I), marketed in tablet form, which is adapted
to the liquid form in order to be administered to children causing the loss of
efficacy and safety of the drug. Objective: The objective of this study was
to evaluate the compatibility between the captopril and possible excipients
(citric acid anhydrous, carboxymethylcellulose,
hydroxypropylmethylcellulose, EDTA, potassium phosphate,
methylparaben, propylparaben and saccharin) by thermal analysis to
obtain a formulation captopril powder for reconstitution to be used as a
pediatric dosage form. Materials and Methods: About 4 mg of isolates
and 1:1 binary mixtures were placed in alumina pans and analyzed on a
Shimadzu DTG-60 apparatus, under nitrogen flow of 50 mL. min
-1
at a
heating rate of 10 C. min
-1
and the temperature range between 25 and
900C. Results and Discussion: Through the DTA curve of captopril, we
determined an endothermic event at 110C, corresponding to melting point
of captopril. Between 170 and 440C occurs the decomposition of captopril
accompanied by weight loss. According to the curves TG, DTG and DTA,
the excipients present in binary mixtures, for the most part, were
compatible with captopril. The DTA curves of mixtures containing
methylparaben and propylparaben suggest the presence of interaction
between the captopril and preservatives such as the melting point occurred
at 84C and 76C, respectively. Conclusions: Thus, we conclude these
excipients can be used in the formulation, but special attention should be
taken to the performance of the formulations containing the preservative
during the accelerated stability study.


210

CODE 199
INTESTINAL PARASITES IN CHILDREN AND FOOD MANIPULATORS
OF SCHOOL AND RESIDENTIAL OF A MUNICIPAL NURSERY IN
JOO PESSOA-PB

Pontes, Z. B. V.S.; Arajo, J.I. R. Branco U.V.C.; Jnior F.S.F.; Vidal,
E.P.
4
; Dantas I.F.
4
; Filho, W.L.N.;Leo A.D.; Freitas F.A.S.

Department of Pharmaceutical Sciences, Center for Health Sciences,
Federal University of Paraba (UFPB)
Undergraduate in Pharmacy, Department of Pharmaceutical Sciences,
Center for Health Sciences, Federal University of Paraba (UFPB)
Pharmaceutical-Chemistry, Department of Pharmaceutical Sciences,
Center for Health Sciences, Federal University of Paraba (UFPB)
4
Basic Health Unit Nova Conquista(4), the Municipal Health Secretariat of
Joao Pessoa-PB

Introduction: Children still are the most damage beings by intestinal
parasites, mainly because of their lack of hygiene, immunological
development, and, depending on the location, they are submitted to a low
basic sanitation range, and last but not least social and educational
measures. Objective: To diagnose intestinal parasites on children,
households, and school food of the municipal nursery Maria de Lourdes
Gomes at Alto do Mateus in Joo Pessoa PB. That way, promoting
educational attitudes to the community, contributing to reduction of the
prevalence of intestinal parasites, were the aims of this study. Materials
and Methods: The parasitological analysis (methods of Hoffman and
Faust) of 52 children between 3 and six years old, and 34 food
manipulators were made; after an exempt and clarified approval, in the
parasitological laboratory at the Universidade Federal da Paraba. Results
and Discussion: The fecal examinations of 52 children and 29 residential
manipulators showed 71.2% and 62% respectively were parasitized. The
existing school food manipulators not found to be infected. In children were
isolated (multi replication) protozoa: Endolimax nana (41%), Giardia
lamblia (25.5%), Entamoeba coli (17.6%), Entamoeba histolytica (14%)
and Iodamoeba butschlii (1.9%) and / or helminths: trichiura trichiura
(55.6%), Ascaris lumbricoides (33.3%), Enterobius vermicularis (5.6%) and
Schistossoma mansoni (5.6%).The residential manipulators were
parasitized, diagnosed with the following protozoa: : E. nana (45%), E. coli
(20%), E. histolytica (12.5%), G. lamblia (12.5%) and I. butschlii (10%) and
/ or helminths, T. trichiura (40%), A. lumbricoides (30%), Ancylostomatidae
(20%) and Trichostrongylus (10%).The frequency of intestinal parasites
was high in children and manipulator show the need for public policies.
Conclusions: The subjects are in a social context whose economic and
sanitary conditions are precarious, necessitating the continuation of
educational activities, diagnostic and treatment monitoring.

Financial support from UFPB


211

CODE 200.1
CHARACTERIZATION OF SPRAY-DRIED PRODUCT AND
LIOPHILIZED DRIED EXTRACT OF Bauhinia forficata LINK BY
INFRARED SPECTROSCOPY

Lyra, M.A.M.
1
; Peixoto, M.S.
1
; Angelos, M.A.
1
*; Monteiro, R.P.M.; Costa, S.
P.M
1
; Pina, E. M. L
1
; Santos, F. L. A
1
; Alves, L. D. S
1
; Souza, F.S.
2
; Rolim-
Neto, P.J
1
.

1
Universidade Federal de Pernambuco, Departamento de Cincias
Farmacuticas, Laboratrio de Tecnologia dos Medicamentos, Cidade
Universitria, Recife/PE, Brasil
2
Universidade Federal da Paraba, Departamento de Cincias
Farmacuticas, Brasil.

Introduction: The plant species Bauhinia forficata Link (paw-to-cow) is
widely used in the treatment of diabetes mellitus. Infrared spectroscopy
(IR) is a technique of inestimable importance in qualitative organic analysis
and is widely used in the area of chemistry of natural products, organic
synthesis and organic transformations. Objective: Characterized by IR
spectroscopy the spray-dried product and liophilized dried extract. Material
and Methods: The IR spectrum of B. forficata samples was obtained in
FT-IR spectrometer Spectrum 400 from PerkinElmer using an average of
10 sweeps from 650 to 4000 cm-1 with a resolution of 4 cm. Results and
Discussion: The analysis of the IV absorption spectra of the raw
vegetable material, spray-dried product and freeze-dried extract showed
similar bands, indicating the presence of these constituents and that there
were no changes in the spectra after drying the fluid extracts by different
drying techniques . Conclusion: Therefore, we can infer that the drying
processes are suitable for obtaining dried extracts for the development of
solid dosage forms, however, it is necessary to analyze the samples by
complementary analytical techniques and selective for this type of sample
which has a mixture of constituents.

Financial supporting from CNPq/FACEPE


212

CODE 200.2
EVALUATION OF INTERFERING LIPOPHILIC ON DETERMINATION OF
CONTENT OF TOTAL FLAVONOIDS BY SOLID PHASE EXTRACTION

Peixoto, M.S
1
*; Lyra, M.A.M.
1
; Pina, E.M.L.
1
; Angelos, M.A.
1
; Ferraz,
L.R.M.; Monteiro, R.P.M.
1
; Marques, G.S.
1
; Costa, S.P.M
1
.; Santos,
F.L.A
1
; Rolim-Neto, P.J
1
.

1
Laboratrio de Tecnologia dos Medicamentos, Departamento de Cincias
Farmacuticas, Universidade Federal de Pernambuco (UFPE), Recife/PE,
Brasil.
Corresponding author: monize85@hotmail.com

Introduction: Bauhinia forficata Link is a native of South America, used in
the treatment of diabetes mellitus due to antidiabetic activity attributed to
flavonoid compounds present in the leaves. Objectives: Evaluate the
presence of lipophilic interfering in determination of content of total
flavonoids (CTF). Material and Methods: The effect of interfering lipophilic
in the CTF of the extractive solutions was evaluated according to the
alcohol content of liquid extractor, comparing the contents of the
preparations at 50 and 70% (v/v) ethanol:water. O pretreatment of the
sample aiming the retention of interfering lipophilic, was done by solid
phase extraction (C
18
E 80mg/mL) and activated with 3 mL of hydro-
alcoholic solution. The samples were analyzed by spectrophotometry
according to the methodology developed and validated. The same
procedure was repeated to obtain both fractions, without the addition of
AlCl
3
, to obtaining of the compensationsolutions. The absorption spectra of
the fractions in the range 200 to 500 nm were obtained 30 min after the
addition of AlCl
3
.Results and Discussion:The extractive solutions of B.
forficata with 50 and 70% ethanol showed a proportional increase of
metabolites with higher alcohol content. However, this increase in the CTF
is not sufficient to explain the lack of linearity obtained from the
quantification of flavonoids by UV-Vis spectrometry and your marker,
kaempferitrin, quantified by HPLC. Conclusions: However, it was evident
that the extraction solutions analyzed showed no statistical differences in
relation to CTF after the treatment on extraction column on solid phase,
with no significant interfering lipophilic.

Financial supporting from CNPq/FACEPE


213

CODE 201.1
PHYSICAL CARACTERIZATION OF SPRAY-DRIED PRODUCT AND
LIOPHILIZED DRIED EXTRACT OF Bauhinia forficata LINK BY
SCANNING ELECTRON MICROSCOPY (SEM) AND X-RAY
DIFFRACTION.

Peixoto, M. S
1
; Lyra, M. A. M.
1
; Angelos, M. A.
1
; Monteiro, R. P. M.
1
;
Marques, G. S.
1
; Pina, E. M. L.
1
; Lima, A. A. N
1
.; Souza, F. S.
2
; Rolim-
Neto, P. J
1

1
Farmacuticas, Universidade Federal de Pernambuco (UFPE).
2
Departamento de Cincias Farmacuticas Universidade Federal da
Paraba (UFPB).

Introduction: The plant species Bauhinia forficata Link (cows pw) s
widely used in the treatment of diabetes mellitus. Dried extracts have been
used to obtain different dosage forms, in order to obtain products with
higher concentrations of chemical constituents and with better
technological properties. Objective: Caracterize spray-dried product and
freeze-dried extract with analytical techniques. Material and Methods: It
was used a microscope Joel JSM-5900 and a SIEMENS diffractometer
with copper anode, with angle from 1,5 to 90cm with an interval of 2 and
scan speed 0,02 2 / s. Results and Discussion: SEM showed that the
extracts dried by lyophilization showed the presence of resinous aspect of
continuous and irregular clusters. The spray-dried product had porous
spherical particles with sizes between 1 and 10 micrometers. In X-ray
diffraction, both dried materials showed to be typically amorphous, with the
cene of he mophous pek 2 = 21,51. Conclusion: Herbal dried
extracts are essentially amorphous and only spray dried product showed
better defined particles, allowing a better flow during pharmacotechnical
industrial development of solid dosage forms.

Financial support from CNPq, FACEPE/ Brazil.


214

CODE 201.2
EVALUATION OF HYGROSCOPIC IN SPRAY-DRIED PRODUCT (SDP)
and LYOPHILISATE DRIED EXTRACT (LDE) OF LEAVES OF Bauhinia
forficata Link

Peixoto, M. S.
1,2
; Lyra, M. A. M.
1
; Angelos, M. A.
1
; Costa, R. P. M.
1
; Pina,
E. M. L.
1
; Monteiro, R. P. M.
1,3
; Albuquerque, M. M.
2
; Rolim-Neto, P. J.
1

1
2
Ncleo de Controle de Qualidade de Medicamentos e Correlatos,
Pernambuco (UFPE).
3
Laboratrio de Farmacognosia, Departamento de Cincias

Introduction: The determination of the hygroscopicity in raw plant material
is an essay of great technological relevance for the production of herbal
medicines. The high water content promotes the growth of microorganisms
and influence pharmacotecnic quality of solid materials, which can affect
the rheological characteristics and physical and chemical stability.
Objective: To assess the hygroscopicity of SDP and LDE from Bauhinia
forficata Link. Material and Methods: The extraction solution was
prepared in the proportion of 1:5 mass plant: volume of solvent, with 50%
of hydroethanolic solution, heating at 40C and stirring at 200 rpm for 1
hour. The SDP were obtained with 20 and 30% of colloidal silicon dioxide,
while there was no addition of filler to the LDE. The hygroscopicity of the
products was evaluated in a desiccator saturated with appropriate solutions
(NaCl for RH 69% and BaCl
2
.2H
2
0 for 88% RH) every 2 days for 14 days.
Results and discussion: The SDP had lower moisture sorption that the
LDE. At RH 69% the moisture sorption was stabilized after 4 days, LDE
sipped 20.06% and SDP 20% sipped 26.64%. After 14 days at RH 88%
LDP drank 64.3% of moisture, SDP at 20% drank 47.66% and SDP at 30%
drank 43.73%, illustrating a difference in performance of products when
subjected to different humidity conditions . Conclusion: Among the
products analyzed, the SDP had lower hygroscopicity than the LDE, the
latter requiring the addition of adjuvant drying to reduce moisture
adsorption in plant materials.


215

CODE 202
SYNTHESIS, CHARACTERIZATION AND EVALUATION OF THE
LEISHMANICIDAL ACTIVITY OF MEGLUMINE ANTIMONIATE IN
PROMASTIGOTE FORMS OF Leishmania major

Omena, R. J. M.; Meneghetti, M. R.; Melo, G. M. de A. ; Cavalcante, A.
Q.

Grupo de Catlise e Reatividade Qumica, Instituto de Qumica e
Biotecnologia, Universidade Federal de Alagoas (UFAL).
Laboratrio de Farmacologia e Imunologia, Instituto de Cincias
Biolgicas e da Sude, Universidade Federal de Alagoas (UFAL).
Corresponding author: Rafael.Omena@hotmail.com.br

Introduction: Leishmaniasis anthropozoonosis are considered a major
public health problem. Ranked among the top five global parasitic
diseases, leishmaniasis is present in 88 countries on five continents. For
over six decades meglumine antimoniate (Glucantime) is used as a drug
of first choice for the treatment of leishmaniasis, despite its toxicity.
Objectives: This work aims to determine our ability to synthesize, and
evaluate the leishmanicidal and immunomodulating properties of
organometallic compounds of antimony. Materials and Methods: For the
syntheses of meglumina antimoniate, the methodology proposed by
Demicheli et al (2003). The characterization was made by analysis of 1H
NMR and infrared spectroscopies. To evaluate the leishmanicidal activity of
meglumine antimoniate we have carried out feasibility testes with L. major
promastigotes parasites in vitro. Results and Discussion: The data
obtained from the 1H NMR and infrared analysis confirm the formation of
meglumine antimoniate. The meglumine antimoniate failed to inhibit the
growth of forms of L. major promastigotes in any of the concentrations
tested. Conclusion: The synthesis methodology proposed by Demicheli et
al (2003) was quite satisfactory, as well as being simple and rapid
characterization demonstrated the formation of meglumine antimoniate. As
already expected, the product obtained was not able to inhibit the growth of
the parasite, but other biological essays are in progress.


216

CODE 203
INTERACTIONS OF ANTI-HYPERTENSIVE THERAPY DISPENSED BY
THE MUNICIPAL HEALTH NETWORK OF MACEIO / AL

Nascimento, N. M.; Magalhes, Y. M.; Filho, A. D. O.
1
; Vasconcelos, H.
A. L. M.
1

1
School of Nursing and Pharmacy, Federal University of Alagoas
Corresponding author: naty_bum20@hotmail.com

Introduction: The prescription of multiple medicines is a practice
commonly used in therapeutic regimens. Interactions are special types of
pharmacological responses, in which the effects of one or more medicines
are altered by concurrent administration of other or earlier, or by concurrent
administration with food. Several antihypertensive treatments using more
than one medication, and there may be associations with other therapies,
where patients may suffer the consequences of possible interactions
between these medicines. Objective:To identify possible interactions of
antihypertensive medicines present in the Municipal Register of Essential
Medicines (REMUME), provided by the public health service of Macei -
AL. Materials and Methods: It was a literature review about the possible
drug interactions between anti-hypertensive medications dispensed by the
Basic Health Unit Graciliano Ramos-Macei / AL to 198 hypertensive
patients.Results and Discussion: The combined use of atenolol /
propranolol and verapamil may depress myocardial contractility, causing
severe hypotension. Non steroidal Anti-inflammatory medicines reduce the
effects of thiazides, captopril, furosemide, and losartan. Captopril increases
the risk of lithium toxicity. Enalapril increases in serum potassium.
Furosemide causes kidney failure especially when associated with
enalapril, captopril and losartan, and increased toxicity of salicylate.
Thiazides affect on insulin requirements in diabetics, reduce effect of oral
hypoglycemic agents. Methyldopa can lead to manifestations of
idiosyncratic drug and verapamil increases serum digoxin levels and may
result in digitalis intoxication, among others.Conclusions: Should be given
greater attention as the prescription of combination therapy, because the
resulting interactions can be numerous, both positive and potentially
harmful and fatal to the patient.


217

CODE 204.1
USE OF STATISTICAL PROCESS CONTROL AS A TOOL IN
OPTIMIZING THE QUALITY OF THE PRODUCTION OF DIPYRONE 500
MG/ML ORAL SOLUTION DROPS

Malheiro, J. P. T.2; Souza, E. de J.3; Oliveira, A. D. de1; Oliveira, A. R.2;
Farias, S. R. Q de.1; Ramos, S. V. V.1; Oliveira, M. A. C. de1 ; Arajo, E.
S. de.1; Vasconcelos, F. V. C. de1

1
Laboratrio Farmacutico do Estado de Pernambuco Governador Miguel
Arraes (LAFEPE).
2
3
Universidade Estadual de Feira de Santana (UEFS).
Corresponding author: malheirojpt@hotmail.com

Introduction: The search for quality assurance and a good performance of
a production process, has fostered the need to use statistical methods as
tools to optimize the preceding steps in the manufacture and establish
measures to reduce losses, thus leading to improved processes
productive. The Statistical Process Control (SPC) has been used for this
purpose. Objective: Optimize the production process of the oral solution
Dipyrone 500mg/mL drops. Materials and methods: Were analyzed 21
batches of Dipyrone 500mg/mL produced in the LAFEPE in 2009/2010,
using the computer package WinCEP plus 5, to establish the upper and
lower control limit (UCL and LCL, respectively), for the parameters:
content, pH, density and filling volume. Next, were analyzed three
consecutive batches produced in 2010, comparing these results with the
limits established by retrospective analysis. Results and discussion: The
analysis of samples collected in the production process, all met the limits
for content and density, 8.5% of samples (n = 4) were out of the LIC for pH,
although 100% meet the specifications, and 50% remained above the LSC
for the average volume filling, not characterizing a deviation from the
requirements of the legislation. Conclusion: The study ensured that the
production process of Dipyrone meets the quality attributes. The tools of
the SPC revealed sensitivity to detect unusual events demonstrating that
the variables pH and volume filling may be better adjusted to produce more
uniform results.


218

CODE 204.2
ENVIRONMENTAL MONITORING IN THE FILLING LINE DROPS: AN
ANALYSIS OF THE ENVIRONMENT, PRODUCTIN PROCESS AND
FINISHED PRODUCT DIPYRONE 500MG

Malheiro, J. P.T.2; Souza, E. de J.3; Oliveira, A. D. de.1; Oliveira, A. R.2;
Farias, S. R. Q de.1; Ramos, S. V. V.1; Oliveira, M. A. C. de1 ; Arajo, E.
S. de.1; Vasconcelos, F. V. C. de1

1
Arraes (LAFEPE).
2
3
Corresponding author: malheirojpt@hotmail.com

Introduction: The quality and stability of a dosage form depends on their
physical and chemical characteristics as well as the quality control of its
manufacturing process. The presence of microorganisms in the product,
may compromise the final quality or patient safety. In non-sterile, it admits
the presence of microbial load, although limited, given the characteristics of
its use. Objective: Evaluate the environmental conditions in the
manufacture of Dipyrone 500mg/mL oral solution drops. Materials and
methods: We analyzed 03 batches of Dipyrone 500mg/mL sequence drops
produced in LAFEPE in October / November 2010, using as control
variables of temperature, moisture, and microbial counts. This was done
through Rodac Plates plates, enriched, broths, Petri dishes and air
sampling, to the environment, and products in process and finished. Those
who were considered critical points identified and monitored. Results and
discussion: The variables temperature and humidity remained within
specifications during handling and filling of the three lots in the study. The
counting of environmental microbiological load in place of filling in the UFC,
showed levels of contamination above the specified. However, analysis of
the product in process and finished product, quantified in less than 10 UFC
/ mL micro-organisms, fungi and yeast, and identified the absence of
pathogens in three batches. Conclusion: The liquid filling line control to
maintain a good temperature and humidity. The microbial load in the
environment, did not compromise the final product quality drops Dipyrone
oral solution.


219

CODE 205.1
PRELIMINARY EVALUATION OF THE ANTINOCICEPTIVE ACTIVITY
OF THE SESQUITERPENE NEROLIDOL

Carvalho, F. L.
1
; Fonsca, D. V.
1
; Salgado, P. R. R.
1
; Leite, F. C.
1
; Sousa,
D. P.
2
; Morais, L. C. S. L.
1
; Almeida, R.N.
1

1
Universidade Federal da Paraba (UFPB);
2
Departamento de Fisiologia, Universidade Federal de Sergipe (UFS).

Introduction: Medicinal plants are widely studied due to its therapeutic
properties, attributed to the various chemical constituents synthesized by
these plants, among which stands out the essential oils, as nerolidol for
example. Nerolidol is a sesquiterpene with some pharmacological
properties already described, such as antimicrobial, antifungal and anti-
leishmania. Objective: This study aims to evaluate the antinociceptive
activity of nerolidol using the acetic acid-induced writhing test. Material
and Methods: Swiss male mice weighing 25-30 g were divided into groups
of eight animals each and were treated orally (p.o.) with nerolidol (125, 250
and 500 mg/Kg), morphine (10 mg/Kg) and vehicle (Tween 80 and distilled
water). After one hour of initial treatment, the animals received an
intraperitoneal injection of acetic acid 1% to induce the abdominal
contortions, characterized by abdominal constriction followed by extension
of hind legs. During a period of 15 minutes were recorded the number of
abdominal constrictions and latency to the onset of the first twist. The
results were statistically analyzed by ANOVA followed by Dunn's multiple
comparisons test, and the results were considered significant when
p<0.05. Results and Discussion: Nerolidol was able to reduce
significantly the number of abdominal contortions at doses of 250 (21.1
3.1) and 500 (18.1 2.0) mg/kg in the control group (47.8 1.3). The
latency to the first contortion was not different between the experimental
and control groups. Conclusion: These results suggest that nerolidol has
a possible antinociceptive activity. Further tests are needed to confirm this
effect and to elucidate the antinociceptive mechanism.



220

CODE 205.2
SEROLOGICAL STUDY OF CHAGAS DISEASE IN THE CITY OF
SALGADINHO/PB

Carvalho, F. L.1; Fonsca, D. V.1; Vilar, A. C. Q.2; Vilar, A. P. 2; Lima, C.
M. B. L. 2;
Freitas, F. I. S. 2

1
2
Laboratrio de Parasitologia, Centro de Cincias da Sade, Universidade

Introduction: Chagas disease was first described and discovered by the
Brazilian physician Carlos Chagas in 1909. This infection is caused by the
protozoan parasite Trypanosoma cruzi, whose most important vectors are
the species Triatoma infestans, Rhodnius prolixus and Triatoma dimidata.
Almost a century after its discovery, Chagas disease remains a serious
public health problem in Brazil and Latin America. Objective: Given the
lack of data on the number of seropositive individuals for Chagas disease
in the city of Salgadinho, this study aimed to identify these patients and
refer them for treatment. Material and Methods: The study had the
participation of 215 volunteers, 74 from urban areas and 141 rural. Initially,
educational lectures on various topics in health care were performed. After
this step, blood was collected from these participants and submitted to the
indirect hemagglutination and ELISA tests. Results and Discussion:
From 215 volunteers, only 3 (1.4%) presented positive serology for both
techniques applied. Women were more affected with the disease. All
positive cases were aged between 51 to 60 years. The main occupation of
patients with Chagas was agriculture. In relation to schooling, two patients
had elementary school and one high school incomplete. Conclusion: The
identification of infected patients for Chagas disease is of vital importance
to no spread of this illness, making necessary the application of public
measures to prevent new cases.



221

CODE 206
EVALUATION OF PHYSICAL AND CHEMICAL COMPOSITION OF
THREE CULTIVARS OF PAPAYA SEEDS PRODUCED IN THE
NORTHEAST OF BRAZIL FOR POSSIBLE RECOVERY IN FOOD
INDUST

Moreira, A. L.
1
; Carvalho, L. M.
2
; Costa, M. H
3
; Santos, N. A.
4
; Melo, M. L.
S.
1

1
Department of Pharmaceutical Sciences, Federal University of Paraiba
(UFPB).

2
Department of Food Engineering, Federal University of Paraiba (UFPB).
3
Department of Industrial Chemistry, Federal University of Paraiba (UFPB).
4
Master in Chemistry from UFPB;
Corresponding author: alanpb@hotmail.com

Introduction: Brazil is the second largest producer of papaya and Paraba
occupies the fifth place in national production. Utilization of seeds to obtain
a vegetable oil can use food and/or your pie for animal feed. Materials and
Methods: We investigated the physical and chemical composition of seeds
of three cultivars of papaya: Sunrise soil (Hawaii), Golden and Formosa.
Consideration was also to oils obtained, as the fatty acid composition,
physico-chemical and thermal stability. Results and Discussion: Cultivars
Golden and Sunrise solo show in their physical composition a higher seed
yield of 2.54 and 1.43% respectively against 0.615 cultivar Formosa. The
chemical composition of the seeds in g/100 g showed a high protein
content (23.58 to 25.46) fibers (16.07 to 19.19) and minerals (5.12 to 6.48)
among the three cultivars, especially minerals Fe and P, with the greatest
values, respectively, from 104.18 mg / g and 634.12 mg / g for cultivars
Golden and Solo Sunrise. The oil content of three cultivars was 28%
majority being oleic fatty acids with percentages of 69.78, 70.13, and
72.04% and palmitic acid with percentages of 18.95, 18.89 and 18.20,
respectively for the Golden cultivar, soil and Formosa Sunrise. Indices in
the same order of acidity: 1.0, 0.98 and 1.03, Iodine 71.30, 7.26 and 70.45
and saponification: 190,2,190,14 and 189.98. The test of thermal stability
of the oil in an oven (65 C) for a period of 25 days showed a variation of
peroxide value between (1 and 47.8 meq / kg). Conclusions: The results
indicate the high nutritional potential of the pie and oil, whose chemical
composition resembles qualitatively and quantitatively the olive oil,
considered ideal for health maintenance.


222

CODE 207.1
EXTRACTION AND CHARACTERIZATION OF CHITIN FROM SHRIMP
WASTE OF RIO GRANDE DO NORTE - BRAZIL

Santos, S. R. A.
1
; De Souza, I.
1
; Pedrosa, M. F. F.
1
; Fonseca, J. L.
3
; Silva-
Jnior, A. A.
1

1
Department of Pharmacy, Federal University of Rio Grande do Norte
(UFRN).
2
Department of Chemistry, Federal University of Rio Grande do Norte
(UFRN).

Introduction: Rio Grande do Norte (RN) is the both first and largest
shrimp producer of Brazil. The main component of waste from shrimp is its
shell, which is rich in composition of the polymer- (1-4)-N-acetyl-D-
glucosamine, usually called by chitin. Chitin may be used as flocculating
agent in wastewater treatment, as adsorbent in clarification of oils and
mainly for the production of chitosan, a biodegradable copolymer which
has many industrial applications. Objectives: This study aimed to obtain
and characterizing chitin in a laboratory scale from waste of shrimps
produced in RN. Materials and methods: The extraction procedure of
chitin was conducted in several steps: material collection, pretreatment,
demineralization, deproteinization, deodorizing and drying. After obtaining
the biopolymer, it was characterized by thermal analysis
(Thermogravimetry - TG), X-ray diffraction, absorption spectroscopy in the
infrared (IR) and scanning electron microscopy (SEM). Results and
discussion: The thermogravimetric analysis curves showed three stages
of degradation. An inorganic residual material content about 10.19% was
identified. SEM micrographs of chitin showed a homogeneous, compact,
fibrous and is consistent particles The X-ray analysis demonstrated the
obtaining of a crystalline domains polymer. The characteristic stretching
bonds at 3444cm-1 (OH axial stretching OH); 3270cm-1 e 3107cm-1
(NH axial stretching) were observed in IR spectrum. Conclusion: The
physicochemical characterizing confirmed the obtaining of the polymer-
(1-4)-N-acetyl-D-glucosamine.

Financial support from NEPGN, CNPq and PROPESQ-UFRN


223

CODE 207.2
EVALUATION OF THE AUTHENTICITY OF A SAMPLE OF SENNA
MARKETED IN NATAL-RN.

Santos, S. R. A.
1
;

Carvalho, J. R. P.
1
; Mesquita, P. C.
1
; Souza, I.
1
;
Zucolotto, S. M.
1

1
Corresponding author: sarahrafaelly@hotmail.com

Introduction: Senna alexandrina Mill (Leguminosae), popularly known as
Senna, is widely used as a carminative, laxative, purgative and vermifuge
mainly because of the presence of anthraquinones. Frequently, S.
alexandrina is adulterated with other Senna species such as S. auriculata.
Objetive: Then, the aim of this study was to verify the authenticity of a
Senna sample marketed in the city of Natal, Rio Grande do Norte.
Materials and Methods: The quality control analysis (identification,
macroscopic and microscopic tests) of the Senna leaves were done
according with 4
th
edition of the Brazilian Pharmacopoeia (1988). Chemical
identification of anthraquinones was carried out by three methods:
Borntrger Reaction, micro-sublimation test and Thin Layer
Chromatography (TLC). Additionally, reactions to detect flavonoids and
saponins were performed. Results and Discussion: In relation to the
purity tests, the sample showed 1,2% of foreign materials (Pharmacopoeial
standard: 5%). Macro and microscopic analysis showed similar results to
the ones described in the Brazilian Pharmacopoeia, only the size of the
leaves and the presence of essential oils was not in accordance. In the
results obtained by chemical reactions, it was not detected the presence of
tannins and flavonoids, only the presence of saponins and anthraquinones.
In the TLC analysis, it was identified the senoside A and B, according with
the color spots (brown-red) and Rfs (0.57 and 0.65, respectively) observed.
Conclusion: According to our results, the commercial sample of Senna
showed satisfactory results regarding the parameters established for
identification of the drug plant in the Brazilian Pharmacopoeia, which
contributes to the safe use of the drug plant.


224

CODE 208.1
EVALUATION OF QUALITY OF SAMPLES OF Baccharis trimera
MARKETED IN DIFFERENT CAPITALS OF THE BRAZILIAN
NORTHEAST THROUGH HPLC ANALYSIS

Angelos, M. A.
1
;

Ferreira, P. A.
1
; Santos, F. L. A.
1
; Rolim, L. A.
1
; Alves, L.
D. S.
1
; Lyra, M. A. M.
1
; Ferraz, L. R. M.
1
; Peixoto, M. S.
1
; Rolim-Neto, P. J.
1

1
Farmacutica, Universidade Federal de Pernambuco (UFPE).
Corresponding author: matheus_alves7@hotmail.com

Introduction: Baccharis trimera, popularly known as Broom is a native
plant of South and Southeast regions of Brazil widely used in folk medicine
against gastrointestinal problems. Among various secondary metabolites
existent in the plant, flavonoids derivatives of caffeic acid stand out as they
serve as chemical markers of the species. This study aims to determine
the amount of caffeic acid by High Performance Liquid Chromatography
(HPLC) of three commercial samples of "broom" from Recife, Joo Pessoa
and Fortaleza. Objectives: To verify the legitimacy of commercial samples
obtained from "Broom" through the determination of caffeic acid by HPLC.
Materials and Methods: The sample solutions were obtained from 1 g of
dried and milled drug performing extracts with ethanol: water (50:50) at a
temperature of 40C under stirring. The extracts were filtered through
cotton. A rate of 1.2 mL was taken and diluted to 10 mL of an eluent
system. The final solutions were injected into the equipment and the
chromatograms were obtained with UV detector at 325 nm. Results and
Discussion: The chromatograms obtained were similar to the profiles of
caffeic acid in the samples and pattern, differing only in the concentration
of each one. Conclusions: The analysis of samples sold in different
markets demonstrated the legitimacy of products obtained from Baccharis
trimera through the determination of caffeic acid, demonstrating the
popular use of the correct species. The analytical methods used were
appropriate for the determination of caffeic acid in the raw plant material.

Financial support from CNPq, FACEPE/Brazil.


225

CODE 208.2
ANALYSIS OF EXTENDED RELEASE TABLETS BENZNIDAZOLE BY
RAMAN SPECTROSCOPY AND MICROSCOPY

Angelos, M. A.; Lima, A. A. N. ; Almeida, E. C. L.; Silva, C. C. A. R.;
Lyra, M. M. L.; Santos, F. L. A.; Rolim Neto, P. J.

Laboratrio de Tecnologia Farmacutica, Faculdade de Cincias
Farmacuticas, Universidade Federal do Amazonas (UFAM).
Laboratrio de Microscopia, Centro de Tecnologias Estratgicas do
Nordeste (CETENE).
Corresponding author: matheus_alves7@hotmail.com

Introduction: In drug development, the characterization of the raw material
is needed, especially to know the physicochemical properties of the drug to
optimize the development of the pharmaceutical form. The benznidazole
(BNZ) is the only drug used to treat Chagas disease. Objective: To
analyze the drug and tablets by Raman BNZ to check the influence of the
drug and excipients in solid dosage form. Methodology: Was analyzed by
Raman spectroscopy and microscopy, the BNZ, raw material and
extended-release tablets of the drug, two lots of tablets, B1 (25% HPMC)
and B2 (35% HPMC). Results and Discussion: In the analysis of the
Raman spectrum of BNZ, is observed a broad band covering the whole
spectrum, because the fluorescence of molecules in the compound that
absorbs the laser excitation (514 nm). Two characteristic peaks are
observed at 1130 cm -1 and 1360 cm-1. We observed the same peaks in
the spectrum of tablet B1, noting a characteristic fluorescence, since there
are other ingredients in the tablet that showed that fluorescence in the
Raman spectra of each isolated component. This high fluorescence is
more evident still in the Raman spectrum of compressed B2, where the
highest concentration of this polymer further contributed to the high
fluorescence observed in the spectrum. Conclusion: This procedure
allowed obtaining micrometer-resolution spectra, important to evaluate the
behavior of the drug alone and in front of the tablet excipients at different
concentrations, demonstrating a promising technique in the evaluation of
solid dosage forms.

Financial support from CNPq, FACEPE/Brazil.


226

CODE 209.1
DETERMINATION OF KINETIC PARAMETERS BY OZAWA FOR
ALKALOID SYNTETIC WE010

Galvo, F. P.
1
; Silva, E. P.
1
2
; Frana, P. H. B.
2
;
SnAn, A. E. G.
2
; Arago, C. F. S.
1
; Gomes, A. P. B.
1

1
Laboratory Quality Control (LCQMed), Pharmacy Department (DFAR),
Federal University of Rio Grande do Norte (UFRN).
2
Universidade Federal de Alagoas (UFAL).

Introduction: Thermal analysis is applied in the pharmaceutical industry
for determination of the stability of a drug and formulations. The sample
used in this analysis is the type guanilhidrazonas aromatic alkaloids. This
alkaloids were prepared by direct reaction of the respective aldehydes with
aminoguanidine hydrochloride in 95% ethanol reflux using an adaptation of
the method described by Ulrich and Cerami (1984). Objective: The
purpose of this study was to determinate by Ozawa model the following
kinetics parameters: order, kinetic energy and frequency factor for WE010.
Materials and Methods: The curves TG alkaloid WE010 were obtained in
the thermobalance SHIMADZU, model DTG-60, using an alumina crucible,
at 10, 20, 40, 60 and 80 C/ min
1
heating rate up to 900C, under an
atmosphere of nitrogen at 50 mL/ min
1
. It was weighted 7.00.5 mg. The
curves TG were analyzed using TASYS program from Shimadzu, TGA
Kinetic Analysis Program (Ozawa Model). Results and Discussion: The
alkaloid WE010 was analysed by TGA kinetic analysis in the heating rate
10, 20, 40, 60 e 80 C min-1.The following kinetic parameters were found:
9

min-1) fo 0.2. The following kinetic parameters were found: order(0.3),
kinetic energy (90.54 KJ/mol) and frequency factor(9.56 x 10
8
min-1) fo
0.3. Conclusions: Ozawa model equation can be used a suitable
method for evaluate the stability of pharmaceutical, in this case in solid
state.

FAPERN/Brazil.


227

CODE 209.2
THERMAL CHARACTERIZATION OF ALKALOID SYNTETIC WE010 BY
TG/DTA AND DSC

Galvo, F. P.
1
; Silva, E. P.
1
2
; Frana, P. H. B.
2
;
SnAn, A. E. G.
2
; Arago, C. F. S.
1
; Gomes, A. P. B.
1

1
2
Universidade Federal de Alagoas (UFAL).

Introduction: The application of thermal analysis, especially DSC
(Differential Scanning Calorimetry) and TG (Thermogravimetry), has been
of fundamental importance in the study of characterization of the raw
materials and pharmaceutical products, the purity determination and
testing of stability and kinetics of decomposition. Objective: This study
aims to thermal characterization, through curves TG/DTA and DSC of the
alkaloid WE010. Materials and Methods: The alkaloid WE010 was
analyzed by TG/DTA on a SHIMADZU thermobalance, model DTG-60,
using an alumina crucible, at 10 C/ min
1
heating rate up to 900C, under
an atmosphere of nitrogen at 50 mL/ min
1
. It was weighted 7.00.5 mg.
The DSC curves were obtained for SHIMADZU calorimeter, model DSC-
60, using an aluminum crucible, at 10C/min
-1
heating rate up 450C. The
curves were analyzed using TASYS program from Shimadzu. Results and
Discussion: The alkaloid WE010 showed four steps of decomposition.
The first step corresponds the weight loss the humidy. The second step
corresponds the decomposition and onset temperature was 211.36, with
weight loss about 18%. The DSC data showed a melting peak, with onset
189.46 C, Heat 70.08 J/g and purity 97.97%. Conclusions: The thermal
techniques can be used in the characterization and purity determination of
pharmaceuticals.

FAPERN/Brazil.


228

CODE 210.1
PARTICIPATION IN CLINICAL PAIN, FOLLOWING THE TREATMENT
OF ANTIINFLAMMATORY NON-STEROIDAL ONCOLOGY WARD
ASSISTANCE FOUDATION OF PARAIBA FAP

Janurio, M. D. A.
1,2
; Freitas, D. E.
1,2
; Morais, F. L. L.
1,2
; Quirino, T. F.
1,2
;

Santos, A. M. F.
1,2
; Sette, I. M. F.
1
; Belm, L. F
1
.

1
Department of Pharmacy, State University of Paraiba (UEPB).
2
Center of drug information, Campina Grande PB.
Corresponding author: moniquedielly@hotmail.com

Introduction: The oncology develops, so very dynamic, and pharmacists
are challenged to keep informed about new therapies for the development
of an appropriate pharmaceutical care. It is important to the monitoring of
hospitalized patients who make use of therapy with nonsteroidal anti-
inflammatory drugs (NSAIDs), to be a pharmacological class of drugs
widely used in hospitalized patients in order to provide greater patient
comfort by relieving symptoms and especially pain and inflammation.
Objective: To evaluate the use of NSAIDs in patients hospitalized in the
oncology center promoting the rational use of medicines. Materials and
Methods: Cross-sectional and quantitative approach in patients
hospitalized in the oncology ward through the analysis of medical records.
Results and Discussion: We analyzed 134 patients in the ward at the
Hospital da Fundao Assistencial da Paraba - FAP during the period
January to July 2011. There are diverse types of diagnoses, the most
prevalent cancer of the prostate (17%) and cervical cancer (14.8%). Of the
800 drugs used, distributed across 18 therapeutic categories, NSAIDs
appear to 17.4% between these Dipyrone appears as the most prescribed
NSAIDs most prescribed on the oncology ward with 95%. 9.7% of the
patients studied had at least one RAM. Conclusions: The monitoring and
drug safety are essential to the effective use of medicines and
pharmaceutical care provision, particularly also in cancer patients, who due
to their compromised physical state sets as a patient in risk to predispose
Adverse Reactions Medicines (RAM).



229

CODE 211
THE QUEST FOR ION CHANNEL MEMORY USING A LIPID
MEMBRANE

Egito, E. M. N.
1, 2
; Escorel-Neto, A. C.
2
; Andrade, C. A. S.
3
; Cassia-Moura,
R.
1, 2, 4

1
Escola Politcnica, Universidade de Pernambuco (UFPE).
2
Laboratrio de Modelagem Biolgica, Instituto de Cincias Biolgicas,
Universidade de Pernambuco (UFPE).
3
Centro Acadmico de Vitria, Universidade Federal de Pernambuco
(UFPE).
4
International Centre for Theoretical Physics, Trieste, Italy.
Corresponding author: rcassiamoura@hotmail.com

Introduction: Dynamic activation of ion channels formed by colicin Ia
incorporated into a planar bilayer lipid membrane (BLM) was investigated
by the voltage clamp technique using different step voltage stimuli.
Objective: To control the dynamic activation of the ion channels by a
specific sequence of pulses used for electrical stimulation. Materials and
Methods: Lipid monolayers were formed using azolectin (L- -
phosphatidylcholine type II - Sigma Chemical Co.) dissolved in hexane, so
that solutions of 1% concentration were obtained. The aqueous solution
consisted of 500mM KCl + 5mM CaCl
2
+ 5mM HEPES + 1mM EDTA (final
pH 7.00). Colicin Ia was added directly to the aqueous solution in one half
of the device attaining a final concentration of 1-
electrodes were used to connect the equipment to the solutions (one
electrode in each half) via salt bridges (2.5% agar in the chamber medium,
electrodes immersed in 3M KCl). Results and Discussion: We have
c
, between two identical
c
, it
produces a predictable current response. On the contrary, if the second
c
, the current response cannot be reliably
predicted. Conclusions: The behavior of the ion channels, when the
c
, may be interpreted as a transient gain or loss
or resetting of memory, as revealed by a specific sequence of electrical
pulses used for stimulation.

Financial support from CNPq/Brazil, FACEPE/Brazil; master
scholarship from CAPES/Brazil.


230

CODE 213
PREPARATION OF DAPSONE-SILICATE NANOCOMPOSITES

Silva,T. A.
1
; Grillo, L. A. M.
2
; Dornelas, C. B.
1

1
Laboratrio de Tecnologia e Controle de Medicamentos (LabTCoM),
Escola de Enfermagem e Farmcia, Universidade Federal de Alagoas
(UFAL).
2
Laboratrio de Biocincias Farmacuticas (LabBFar), Escola de
Enfermagem e Farmcia, Universidade Federal de Alagoas (UFAL).
Corresponding author: tamires_a@hotmail.com

Introduction: According to OMS, Brazil is the second country with the
ges numbe of Hnsen s dsese cses. Dpsone s used as a drug of
choice for its treatment, having a high permeability and low solubility in
aqueous media, so currently formulations to increase its oral solubility are
being sought. In this context, nanotechnology is already being successfully
used. The aim of this work is the use of nanocarrier (silicate) associated
with dapsone, aiming the improvement of the formulation. Objective: To
prepare dapsone-silicate nanocomposites. Materials and Methods: The
preparation of the nanocomposites followed the solvent method.
Throughout the process, were evaluated: the type of silicate (organophilic
or sodium clay), the dapsone-silicate relation (2:1,1:1,1:2) and the time of
agitation (2, 24, 48 and 72hs) to establish the best condition. For that, the
intercalation/adsorption efficiency indirect was calculated by
spectrophotometry. Results and Discussion: In the studied conditions,
the sodium clay yielded better results for dapsone than the organophilic
clay. The result suggests that the used solvent, destilated water ph 2.0,
leads to the drug ionization, with consequent increase in its affinity for the
nanocarrier. Still, the best dapsone-silicate proportion was 1:2 by agitation
for 24hrs, showing a 77.44% 2.85 intercalation/adsorption efficiency.
After this time, in hs popoon, he effcency doesn show ny sgnfcn
increase. Conclusions: The results indicate the formation of sodium
dapsone-clay composites with a relevant value of intercalation/adsorption
efficiency. As a perspective, we intend to evaluate the type of the obtained
nanocomposite by techniques such as XRD and TEM.



231

CODE 214
INFLUENCE OF HIGH GLUCOSE CONCENTRATION ON BONE
METABOLISM IN MICE C57BL/6 INDUCED WITH HIGH-FAT DIET

Bortolin, R. H.
1
; Santos, F. M.
1
; Oliveira, A. M. L.
1
; Freire-Neto, F. P.
1
;
Bezerra, J. F.
1
; Morais, L. V. F.
1
; Ururahy, M. A. G.
1
; Barbosa, A. C. S.
1
;
Lemos, B. S.
1
;

Brito, F. A. P.
1
; Almeida, M. G.
1
; Himelfarb, S. T.
2
; Hirata, R.
D. C.
2
; Hirata, M. H.
2
; Rezende, A. A.
1

1
Department of Clinical and Toxicological Analyses, Federal University of
2
Departament of Clinical and Toxicological Analyses, University of So
Paulo (USP).
Corresponding author: adrirezende@yahoo.com

Introduction: T2DM is a progressive disease caused by both impaired
insulin secretion and by insulin resistance. The diet-induced obesity in
C57BL/6 has common characteristics of the human metabolic syndrome.
Epidemiological studies have suggested that skeletal fragility is increased
in T2DM. Objective: Evaluation of bone profile in male C57BL/6 mice
induced to develop high glucose concentrations in a period of 32 weeks.
Materials and Methods: Adults male C57BL/6 mice were divided in two
groups: control (C, n=4) and diabetic induced by high-fat diet (DH,n=4). We
evaluated body weight, capillary glucose concentration, activity of ALP total
serum, and gene expression of RANK,RANKL,OPG PPAR-,IL-6,TNF-
and PTH in animals femurs. Results and Discussion: The result showed
that body weight was increased for DH (p=1.0e
-3
) compared C and similarly
capillary glucose concentration was increase in DH (p=3.0e
-2
) compared to
C, indicating a possible induction of insulin resistance characteristic of
T2DM. In bone metabolism evaluation the ALP activity for DH was
increased when compared to C (p=2.4e
-3
) and genes expression of
RANK,RANKL,IL-6 and TNF- showed eeved o DH comped wh C
(p=4.0e
-2
;p=3.0e
-4
;p=1.0e
-2
;p=1.0e
-2
respectively), suggesting an onset of
inflammatory process and unbalance on bone metabolism in favor of bone
reabsorption. The PTH, OPG and PPAR- expesson dd not show
significant difference. Conclusions: These results suggest that high fat-
diet may increase the concentrations of glucose leading to an inflammatory
process and consequently to possibly increasing bone reabsorption.

Financial support from FAPESP and CNPq; Scholarship from CNPq
and CAPES/Brazil.


232

CODE 215.1
CLINICAL AND EPIDEMIOLOGICAL ANALYSIS OF SCORPION
STINGS IN CAMPINA GRANDE: PART II

Costa, D. B.
1
; Melo, M. M. A.
1
; Cartaxo, N. A. O.
1
; Soares, N. S. C.
1
; Maris,
S. R.
2
; Fook, S. M. L.
1

Toxicological information Service Center of Campina Grande (Ceatox
CG)/ Departamento of Pharmacy, State University of Paraba (UEPB).

2
Ceatox CG/ Health Sciences Academic Unit, Federal University of
Corresponding author: dayaneb.costa@hotmail.com

Introduction: Scorpionism is a public health problem in Brazil due to its
high incidence rate in certain regions, and its ability to cause severe injury
especially in children. Objective: Outline the epidemiological and clinical
profile of scorpion stings notified at Ceatox-CG in 2009 and 2010.
Methodology: Cross sectional retrospective study following a qualitative
and quantitative approach. Data were collected through records provided
by the Sistema Nacional de Notificao de Agravos (Sinan). Results: The
sample considered 1168 patients, 61% (n=712) females. Prevalent age
group consisted of individuals between 20 and 29 years old (20,5%). Most
patients (88,8%) live in Campina Grande, 89,2% belonging to urban areas
whilst 9,3% were in the countryside. The time elapsed between the bite
and medical care was registered between 0 to 1 hour in 54,9% of the
cases, being the foot the most frequent anatomic area affected (30,9% of
the cases). A total of 97,3% of the patients presented local manifestations,
55,5% pain and 22,6% swelling. An amount of only 11,1% of the cases
presented systemic reactions like headache (30%), vomiting (26,9%) and
diarrhea (10%). Serotherapy was prescribed in eleven cases, nine being
moderated and two being severe. All cases evolved to cure. Conclusion:
Treatment in the first hours is critical in determining the severity of the
cases. It is necessary to effectively combat the areas that enable the
proliferation of the scorpion, especially the urban area, by raising public
awareness through educational campaings.


233

CODE 215.2
SCORPIONIC AND OPHIDIAN ACCIDENT EPIDEMIOLOGIC PROFILE
NOTIFIED IN CAMPINA GRANDE CITY.

Costa, D. B.
1
; Santos, R. L.; Fook, S. M. L.; Mariz, S. R.; Melo, M. M. A.
1
;
Costa, M. M. C.

Toxicological information Service Center of Campina Grande (Ceatox
CG)/ Departamento of Pharmacy, State University of Paraba (UEPB).

2
Ceatox CG/ Health Sciences Academic Unit, Federal University of
Corresponding author: dayaneb.costa@hotmail.com

Introduction: Accidents by snakes and scorpions are problems of
Epidemiological Surveillance, Health Monitoring and Health Care. In Brazil,
these accidents are mostly seen and reported by the Centers and Poison
Information Service. Objective: To determine the epidemiological profile
accidents between the years 2006 to 2008, in the Ceatox-CG.
Methodology: Transversal retrospective study, quantitative and
descriptive of all cases of snakebites and scorpion in Ceatox-CG, between
the years 2006 to 2008. Data were collected through records provided by
the Sistema Nacional de Notificao de Agravos (Sinan). The variables
evaluated were: gender, age, area of occurrence, time between the event
and the service and causing the specimens. Results: In the Analysed
period, had notified 723 ophidian accidents cases and 948 scorpion stings
cases. The ophidian accidents are prevalent in the rural area with 77%
male gender habitants and 55% female gender. About the scorpionics
accidents, its events that happen so often in the urban area being 93.7%
male gender and 58.7% female gender cases. The ophidian and scorpions
accidents its often in persons in the age of 20 to 29 years old. With
respect to gravity are most lightweight type and, the patients are attended
in the hospital unity until 3 hours. The responsible specimens for the big
occurrence, its Bothropoides erythromelas (jararaca) e o Tityus stigmurus
(scorpion). Conclusion: The obtained results in this research its tools
that make possible a strategic formulation of prevention, improvement in
the Health Assistance Services and notified systems of evaluation.


234

CODE 216
HYPERTENSION: EDUCATIONAL ACTIVITY IN PUBLIC HEALTH

Bastos, M. L. C
1
; Silva, M. C. M. da
1
; Campos, D. M.
1
; Cardoso, E. T. C.
1
;
Luz, D. A. da Pinheiro, A. M
1
; Pontes, A. C. A. A
1
; Santos, C. C. dos
1
;
Sarmento, R. M
1
; Silva, E. D. V; Silva, J. V. S.
1
; Silva, L. C. e
1
; Silva, M. L.
da; Dolabela, M. F.
2

1
Pharmacodinamic Laboratory, Federal University of Par (UFPA).
Corresponding author: mirian.c.bastos@hotmail.com

Introduction: The World Health Organization defined hypertension as "a
disease characterized by a chronic elevation of systolic blood pressure and
/ or diastolic blood pressure" The Ministry of Health of Brazil show that the
cardiovascular disease are the first cause of mortality in the country
Because it is a public health problem of high prevalence and low control
rates, and has risk factors (RF) the PET-Pharmacy bothered to do
educational action with hypertension topic. Objective: Promove
educational activities about Hypertension and its prevention. Materials and
Methods: The activity in UBS in a neighborhood on the outskirts of Belm
city of was held with formulary designated to population for check your
knowlege on topic and guidance with brochure explanatory delivering.
Results and Discussion: Of 36 patients, 72,2% cm o know wh is
hypeenson, 33.3% are unaware of their symptoms and only 22.7% know
more than three symptoms. Related to RF, 75% are unaware and 15%
"somewhat knowledgeable" less than 35% are aware of more
than three factors. About controlling hgh bood pessue, e knowedge
were aware, because of the 58.33% who said they know how to keep
constant, the majority cited the reduction of salt as a factor in improving
treatment. Conclusion: It was apparent the need for knowledge about
hypertension, which is worrying, as 66.6%respondents have relatives with
hypertension, which worsens when only 25% say they receive guidance
from health professionals. Was obtained orientation and collaboration of
population by generating discussion questions on the subject.

Financial support from PROEG,UFPA/Brazil.


235

CODE 218.1
ANALYSIS OF DIFFERENT BATCHES OF BENZIDAZOLE BY HPLC
AND FTIR

Ferraz, L. R. M.
1
; Lima, A. A. N.
1,2
; Santos, F. L. A.
1
; Lyra, M. A. M.
1
;
Soares-Sobrinho, J. L.
1
; Rolim-Neto, P. J.
1

1
2
Laboratrio de Tecnologia Farmacutica, Faculdade de Cincias
Farmacuticas, Universidade Federal do Amazonas (UFAM).
Corresponding author: lrapha@hotmail.com

Introduction. The benznidazole (BNZ) is an antiparasitic drug used in the
treatment of Chagas disease. The characterization of the active ingredient
should be the first step in obtaining pharmaceutical forms. One of the
echnoogc boenecks n hs obnng s he dffeence beween
batches of physical-chemical properties inherent to the drug. Objective.
The aim was to characterize the different batches and investigate possible
differences between them, using the techniques of Fourier Transformed
Infra-Red (FTIR) and High Performance Liquid Cromatography (HPLC) as
identification methods. Materials and Methods. Was analyzed, by FTIR and
HPLC, the BNZ three different batches. The LC method involved a C18
column and a mobile phase comprising of ACN:water (1:1 v / v) at 1mL.min-
1 (321m). Results and Discussion. The spectrogram was analyzed relating
the characteristic peaks, particularly the typical bands of the amide (N-H
stretching vibration), carbonyl stretching (amide I band) and N-H deformation
(amide II band), and the vibrations due to the benzyl group and imidazole.
The results of the HPLC chromatograms showed a characteristic
chromatographic peak of BNZ in a retention time around 4.1 minutes, for the
3 lots analyzed. Was also determined the purity of the three lots, which was
99.99%. Conclusions. The analysis of FTIR served as a test for
identification of the lots, proving that it is the same substance, due to all the
bands was placed on the same wavelength. The results obtained by HPLC
showed that each lot has the quality assured and are ready for the
development of pharmaceutical forms.

Financial support from CNPq and FACEPE/Brazil.


236

CODE 218.2
UTILIZAO DE TCNICAS ESPECTROMTRICAS PARA A
CARACTERIZAO DO 3-(4-CLORO-BENZIL)-5-(4-NITRO-
BENZILIDENO)-IMIDAZOLIDINA-2,4-DIONA (LFPS/FZ4)

Ferraz, L. R. M
1
; Costa, S. P. M.
1
; Medeiros, G. C. R
1
; Maia-Junior, R. T
1
;
Rolim, L. A
1
; Rolim-Neto P. J
1
; Silva K. E. R.
1
; Lima M. C. A.
3
, Pitta I. R.
3
;
Galdino S. L.
3

1
Laboratrio de Tecnologia dos Medicamentos (LTM), Departamento de
Farmcia, Universidade federal de Pernambuco (UFPE).
2
Faculdade de Farmcia da Universidade Federal do Par (UFPA).
3
Laboratrio de Planejamento e Sntese de Frmacos (LPSF),
Departamento de Antibiticos, Universidade federal de Pernambuco
(UFPE).
Corresponding author: lrapha@hotmail.com

Introduction. The prototype 3-(4-chloro-benzyl)-5-(4-nitro-benzylidene)-
imidazolidine-2 ,4-done (LPSF/FZ4) s n mdzodne devve nd s
a part of a huge class of bioactive compounds that presents an important
activity schistosomicidal. Objectives. The objective of study was to
characterize by spectrometric techniques a novel molecule (LPSF/FZ4)
aiming at the standardization of raw materials. Materials and Methods.
The LPSF/FZ4 (Batch: 1, 98.00%) was provided by Laboratrio de Sntese
de Frmacos - UFPE. Spectrometric analysis were performed in the infrared
(IR), in the ultraviolet (UV), hydrogen nuclear magnetic resonance (
1
H NMR)
and mass spectrometry (MS / IT-TOF). Results and Discussion. The IR
data identified the presence of major functional groups: the typical bands of
amides (1685 cm
-1
and 1661 cm
-1
) representing the imidazole ring present in
the molecule that gives the anthelmintic activity in addition to the nitro group,
considered parasitophorous, observed at positions between 1543 cm
-1
and
1342 cm
-1
. The structural elucidation by analysis of
1
H NMR was
complementary to IR, identifying the hydrogens of the aromatic groups that
presents chemical shifts in the range of 7.3 to 8.2, which confirms the
position para of the substituents to the molecule. The molecule showed a
specific pattern of absorption in the UV-Vis at 346nm. The mass spectrum
was peculiar, and can detect the gross mass of the prototype in [M-1] =
356.0250 m/z. Conclusions. Therefore, the characterization was of
fundamental importance, because it allowed the structural elucidation of
LPSF/FZ4, providing relevant information for rational drug design.

Financial support from CNPq and FACEPE/Brazil.


237

CODE 220
IDENTIFICATION AND ANTIMICROBIAL SUSCEPTIBILITY OF
ENTEROCOCCI ISOLATED IN THE CITY OF NATAL / RN, BRAZIL

Costa, M. K. C. M.
1
; Melo, M. C. N.
2
; Cidral, T. A.
2
; Silva, S. S. P.
2
;
Merquior, V. L. C.
1

1
Laboratrio de Bacteriologia, Departamento de Microbiologia, Imunologia
e Parasitologia, Universidade do Estado do Rio de janeiro (UERJ).
2
Laboratrio de Bacteriologia Mdica, Departamento de Microbiologia e
Parasitologia, Universidade Federal do Rio Grande do Norte (UFRN).
Corresponding author: mizia.martins@gmail.com

Introduction: Enterococci are ubiquitous organisms, common
gastrointestinal and genitourinary tract. However, they are opportunistic
pathogens causing mainly urinary tract infections, bacteremia and
endocarditis.Objective: Identify isolates of enterococci at the species level
and evaluate the susceptibility to antimicrobial agents commonly used in
clinical practice. Materials and Methods: Were analyzed 68 clinical
samples, collected from 04 hospitals in the city of Natal/RN, Brazil, from
October 2010 to June 2011. The identification at the species was made by
the conventional tests. Evaluation of susceptibility to most antimicrobials
was performed by disk diffusion method (CLSI, 2011) and vancomycin
resistance by E-test. The antimicrobials tested were: vancomycin,
teicoplanin, ampicillin, tetracycline, fosfomycin, ciprofloxacin, and high
levels of aminoglycosides (gentamicin/HLR and streptomycin/HLR).
Results and discussion: Of the 68 enterococci isolates, 63 (92.65%)
were identified as Enterococcus faecalis and 05 (7.35%) Enterococcus
faecium. In relation to antimicrobials was detected low percentage of
resistance to ampicillin (1.47%), fosfomycin (1.47%) and gentamicin/HLR
(8.82%) and the higher percentage for ciprofloxacin (66.17%), tetracycline
(54.41%) and streptomycin/HLR (32.35%). For vancomycin there was
resistance from 11,76%. However, by E-test were sensitive. The high
antimicrobial sensibility observed is due to the fact that E. faecalis is still a
very sensitive species, but it has a high potential of virulence.
Conclusions: The identification at the species levels and resistance
pattern of these isolates are important for establishing the epidemiology of
infections this microorganism in hospitals surveyed well as, to assist
possible preventive measures to control infections clinically important.

Financial support from master scholarship from CAPES/Brazil.


238

CODE 222
HUMAN LEUKOCYTE ANTIGEN-G AND -E EXPRESSION IN GASTRIC
SPECIMENS IN PATIENTS WITH Helicobacter pylori

Souza, D. M. B. O.
1
; Reginaldo, F. P. S.
1
; Giabardo, T.
2
; Soares, C. P.
2
;
Rocha, K. B. F.
3
; Oliveira, C. N.
3
; Jatob, C. A. N.
3
; Donadi, E. A.
4
;
Crispim, J. C. O.
1

1
Department of Toxicology and Clinical Analysis, School of Pharmaceutical
Sciences, (UFRN).
2
Department of Clinical Analysis, School of Pharmaceutical Sciences,
University of So Paulo State (UNESP).
3
Department of Pathology, School of Medicine of University Federal Rio
Grande do Norte State (UFRN).
4
Division of Clinical Immunology, School of Medicine of Ribeiro Preto,
University of So Paulo (FMRP-USP).
Corresponding author: dalianas@gmail.com

Introduction: Human leukocyte antigen-G and -E (HLA-G and HLA-E) are
a non-classical major Histocompatibility complex class Ib molecules that
acts as a specific immunosuppressor. Some studies have demonstrated
which HLA-G and HLA-E seems to be involved in inflammatory diseases,
cancer and viral infections. However, yet nothing is known about the
relationship of these molecules in bacterial infections. The present study is
the first to explore the HLA-G and HLA-E expression profile in gastric
tissue of H. pylori -infected patients. Objective: Determine if HLA-G and
HLA-E are expressed in gastric specimens and if the expression is
associated with clinicopathological data involved in H. pylori infection.
Materials and Methods: We performed a cross-sectional study,
systematically comparing the qualitative expression of the HLA-G and
HLA-E in individuals harboring H. pylori correlating with clinical variables.
Fifty biopsies of the patients with a diagnosis of infection by H. pylori
were evaluated by immunohistochemistry using the primary
monoclonal antibody anti-HLA-G and anti-HLA-E (EXBIO). Results and
Discussion: We detected HLA-G molecules in 46 cases (92%) of 50
patients with H. pylori. HLA-E molecules were detected in 42 cases (84%).
50 (100%) specimens presented chronicity, 49 out of 50 (98%) presented
changes in repair, 45 out of 50 (90%) presented inflammatory activity.
Conclusions: HLA -G and -E status plays an important role in the clinical
outcome of H. pylori infection. These findings are important; however,
additional studies are necessary to investigate the possible involvement of
the molecules HLA-G and HLA-E in the pathogenesis of infection with H.
pylori.


239

CODE 223
COST-EFFECTIVENESS OF ANTIHYPERTENSIVE THERAPIES IN
BASIC HEALTH SYSTEM

Silva, J. P. R.
1
; Flix, B. F.
1
; Lacerda, D. P
1
; Batista, R. S. de A.
1
; Ferreira,
E. V. G.
1
; Barros, A. R. A.
1
; Barbosa, F. O.
1
; Viana, M. de S.
1
; Soares, R.
A.
1
; Silva, P. H. A. da
1
; Alves, H. da S.
1
; Queiroz, I. R. de
3
; Oliveira, M. F.
de
3
.

1
State University of Paraiba (UFPB).
2
Federal University of Campina Grande (UFCG).
3
Nurses, Basic Health System, City of Campina Grande-PB
Corresponding author: farm.joanda@hotmail.com

Introduction: Systemic Arterial Hypertension (SAH) is a multifactorial
clinical condition characterized for high and sustained levels of Blood
Pressure (BP). It is commonly associated to functional alterations and/or
structural alterations in specific organs (heart, brain, kidneys and blood
vessel) and metabolic alterations, with a bigger cardiovascular risk, fatal or
not. SAH has a high prevalence, reaching 22, 3% to 43, 9% of Brazilian
population and it is responsible for high medical and socioeconomic
coasts. Objective: To evaluate cost-effectiveness of antihypertensive
therapies in basic health system. Materials and Methods: Transversal
study, realized from July to September, 2011, in two public basic health
unities, in Campina Grande, Paraba. 359 hypertensive patients were
evaluated. The Blood Pressure was obtained and evaluated by Brazilian
guidelines. Data was typed twice and analyzed by the use of software Epi
Info 3.5.2. Results and Discussion: After analyzing data, 72% patients
were in polytherapy and 56% had BP controlled satisfactorily. The most
used therapeutic diagram were association between diuretic and
angiotensin-converting enzyme inhibitors (ACEI) and monotherapy with
diuretic or ACEI. The mensal coast with therapy was R$ 757,00.
Conclusions: As the patients are in the third age and use polytherapy,
analyzing the farmacotherapy is necessary and correcting some problems
related to medications, wich can result in collateral reactions and bad
control of BP levels, increasing the risk of a cardiovascular disease.


240

CODE 224.1
SURVEY OF RISK FACTORS FOR PREVALENCE OF CASES OF TYPE
2 DIABETES MELITTA IN THE CITY OF ARARA-PB

Freitas, W. S.
1
; Alves, R. D.
1
; Alves, L. D.
2
; Abreu, V. M.
1
; Pedrosa, A. R.
S.
1
; Pedrosa, R. S.
1
; Venceslau, E. M.
3
; Filho, G. V. P.
4

1
Departamento de Farmcia, Universidade Federal de Campina Grande
(UFCG).
2
Departamento de Farmcia, Universidade Federal do Rio Grande do
Norte (UFRN).
3
Departamento de Enfermagem, Universidade Federal de Campina
Grande (UFCG).
4
Graduado em Farmcia.
Corresponding author: wendeberto@gmail.com

Introduction: Diabetes mellitus (DM) is a chronic metabolic disease
characterized by high blood glucose (hyperglycaemia) resulting from
defects in secretion and or insulin action, affecting much of the population
worldwide and is associated with morbidity and responsible for acute and
chronic complications. It is worth noting that most of the subjects with DM,
unaware of their condition, and thus do not perform any type of treatment.
Objective: This study aimed to evaluate the quality of life of patients with
diabetes who live in the city of Arara, PB, listing the key risk factors.
Materials and Methods: According to data from the municipality
HIPERDIA there are about 221 diabetic patients enrolled in the program,
they receive monthly monitoring by primary care through medical and
nursing, and made periodic visits by Community Health Agents (CHA). It a
study of exploratory and descriptive approach with qualitative and
quantitative, which were made periodic visits to health clinics and watched
closely as they were made the calls from patients. Data were collected
from January to March 2011, using the free and systematic observation.
Results and Discussion: The results showed that the prevalence of the
disease is directly related to risk factors, where the eating habits and
physical activity exerts a powerful influence on the energy balance.
Conclusions: Diets with high energy density associated with a sedentary
lifestyle are appointed as the main etiological factors of the increasing
prevalence of obesity in the world.


241

CODE 224.2
EPIDEMIOLOGICAL SURVEY IN CASES OF HEPATITIS TOWN OF
CUITE - PB, BRAZIL, 2011

Freitas, W. S.
1
; Alves, R. D.
1
; Alves, L. D.
2
; Abreu, V. M.
1
; Pedrosa, A. R.
S.
1
; Pedrosa, R. S.
1
; Venceslau, E. M.
3
; Filho, G. V. P.
4

1
Departamento de Farmcia, Universidade Federal de Campina Grande
(UFCG).
2
Norte (UFRN).
3
Departamento de Enfermagem, Universidade Federal de Campina
Grande (UFCG).
4
Graduado em Farmcia e Secretrio Municipal de Sade de Cuit-PB
Corresponding author: wendeberto@gmail.com

Introduction: Viral hepatitis comprises a serious public health problem,
both at the level of Brazil, the world and is thus considered an important
public health issue. There are at least six virus, named A through E, G, and
a newly discovered virus. Primary care teams have an important role in the
diagnosis and monitoring of people with hepatitis, the major route of
transmission of the virus is fecal-oral, inter-human contact or through
contaminated food and water. The spread is related to the socioeconomic
status of the population, there are regional variations in endemicity
according to the degree of sanitation, hygiene education and hygiene
conditions of the population. Objective: The study aimed to survey a given
unit of health of the city of Cuite-PB approached a profile of incidence of
this problem in this region, being an enabling environment for expression of
this virus. Materials and Methods: The methodology was based on
quantitative research, conducted from January to June 2011. Results and
Discussion: The Municipal Health observed that this region had only two
cases of viral hepatitis, although the region is little developed and people
are more exposed the virus. Conclusions: Epidemiological surveillance of
viral hepatitis in Brazil uses the universal system and liabilities, based on
the reporting of suspected cases of confirmed cases and outbreaks of viral
hepatitis, so it should be noted that this reporting is being done correctly to
see whether the data collected is in fact the index profile of hepatitis in the
city of Cuite.


242

CODE 225
USE OF BABASSU IN THE ADSORPTION OF CHEMICALS FOR
WASTE TREATMENT IN INDUSTRY CHEMICAL AND
PHARMACEUTICAL

Silva, G. M. S
1
; Lima, H. R. S.
1
; Cruz, E. T. L.
1
; Nunes, L. C. C.
2

1
Laboratory of pharmaceutical technology, Department of Pharmacy,
Federal University of Piau (UFPI).
2
Nucleus of Pharmaceutical Technology, Department of Pharmacy, Federal
University of Piau (UFPI).

Introduction: The simple presence of residual chemicals/pharmaceuticals
in the aquatic environment can cause noticeable adverse physiological
effects in humans and animals. In view of this, needs some methods to try
control its elimination, among these methods highlight the adsorption.
Objective: Due to the adsorption capacity, as reported, of the derivatives
of babassu and its geographical abundance, this study was carried out in
order to verify the adsorption capacity of the mesocarp, coal and activated
charcoal from babassu. Materials and methods: For preparation of
activated carbon, took a sample of crushed coal, added phosphoric acid
0,1 N later the system was neutralized with potassium hydroxide 0.1 N and
for full activation, took it to the hothouse at 85 C. Each procedure lasted
24 hours and always under agitation and changing a procedure for the
other held a filtration and a wash to stabilize the pH. To check the
adsorption of activated charcoal compared to the crushed coal and
babassu, took an aliquot of each component and added a standard
solution of methylene blue, agitating the new systems for 24 hours. Then,
filtered systems and performed the spectrophotometric scan. Results and
discussion: The potential for adsorption of the three systems was
growing. According to the spectrophotometric reading, the system with
babassu mesocarp resulted in a percentage adsorption equal to 89%, the
system containing crushed coal, 94% and containing activated charcoal,
97%. Conclusion: The activated charcoal from babassu is a powerful
adsorbent and may be useful in cleaning up industrial waste.



243

CODE 226
HUMAN LEUKOCYTE ANTIGEN-G AND TH17 EXPRESSION IN
CERVICAL INTRAEPITHELIAL NEOPLASIA

Miranda, L. N.
1
; Soares, E. G.
2
; Souza, D. M. B. O.
1
; Oliveira, A. E. F.
1
;
Rocha, K. B. F.
3
; Soares, C. P.
4
; Silva, T. G. A.
4
; Donadi, E. A.
5
; Crispim, J.
C. O.
1

1
Department of Toxicology and Clinical Analysis, School of Pharmaceutical
Sciences, University Federal Rio Grande do Norte (UFRN).
2
Department of Pathology, School of Medicine of Ribeiro Preto, University
of So Paulo, (FMRP-USP).
3
Department of Pathology, School of Medicine of University Federal Rio
4
Department of Clinical Analysis, School of Pharmaceutical Sciences,
University of So Paulo (UNESP).
5
Division of Clinical Immunology, School of Medicine of Ribeiro Preto,
University of So Paulo (FMRP-USP)
Corresponding author: lidyaneneves@hotmail.com

Introduction: Cervical intraepithelial lesions are one of the most frequent
causes of cancer among women worldwide. The discovery of early
diagnostic markers has been a central subject. Human leukocyte antigen-G
(HLA-G) is a non-classical major histocompatibility complex class Ib
molecule that acts as a specific immunosuppressor. A new class of T cells
production IL-17 has been identified which plays a critical role in numerous
inflammatory conditions and autoimmune diseases. The present study is
the first to explore the HLA-G and IL-17 expression profile in cervical
biopsies. Objective: Determine if HLA-G and IL-17 are expressed in
cervical biopsies with intraepithelial cervical neoplasia. Materials and
methods: We have evaluated the expression of the HLA-G and IL-17 by
immunohistochemistry in 71 cervical histological samples including 38 low-
grade cervical intraepithelial neoplasia (NIC I), 4 high-grade cervical
intraepithelial neoplasia (NIC II and NIC III) and 29 only with inflammation.
Results and discussion: HLA-G molecules were detected in 38 cases
(53,5%). Among specimens that presented HLA-G expression, 24 (33,8%)
was classified as NIC I, 2 (2,8%) classified as NIC II and NIC III and 12
(16,9%) classified as inflammation. In relation to IL-17 cytokine were
detected in 55 cases (77,3%); 31 (43,6%) was classified as NIC I, 2 (2,8%)
was classified as NIC II and NIC III and 22 (30,9%) classified as
inflammation. Conclusions: In this review we focus utility of specific
biomarkers in identifying true cancerous precursor lesions, additional
studies are necessary to identify the influence of HLA-G and IL-17
expression on cervical neoplasia.


244

CODE 227.1
POSSIBLE ANTINOCICEPTIVE EFFECT OF Lippia microphylla
(VERBENACEAE) ESSENTIAL OIL IN MICE

Penha, A. R. S
1
; Mota, V. G.
1
; Monte, L. O.
1
; Carvalho, F. L.
1
; Tavares, J.
F.
1
1
; Alencar, J. L.
2
; Almeida, R. N.
1

1
Laboratory of Pharmaceutical Technology, Center of Health Sciences,
Federal University of Paraiba (UFPB).
2
Department of Internal Medicine, Federal University of Paraiba (UFPB).
Corresponding author: vanvan_mota@hotmail.com

Introduction: The natural products including medicinal plants have been
established as a main source for obtaining new drugs with potential
therapeutic effect. Species of the Lippia genus are widely used in folk
medicine for treating pain and other ailments. Objective: This study
investigated the antinociceptive effects of the Lippia microphylla essential
oil (LMEO) in animal models. Materials and Methods: All experimental
protocols were approved by the local Ethics Committee for Animal
Research (CEPA N: 0304/08). Swiss mice, albino, male, weighing
between 30-40g, 3-month-old, divided into groups (n= 8 animals) were
treated intraperitoneally (i.p.) to perform: 1) formalin test
1
and 2) hot plate
test
1
: LMEO (50, 100, 150 mg/kg), vehicle, 10 mg/kg morphine. Results
and Discussion: In the formalin test, mice treated with LMEO in the three
doses tested (50 mg/kg: 44.3 5.8; 100 mg/kg: 26.6 2.2; 150 mg/ kg:
12.7 4.6 s.) decreased the licking-time in the first phase of the test,
compared to control animals (65.2 5.5 s.). In the second phase, doses of
50 mg/kg (64.7 25.2 s.), 100 mg/kg (30.3 18.1 s.) and 150 mg/kg (7.3
7.3 s.) reduced the licking-time compared to control (139.3 17,4 s.). The
time of response to the thermoceptive stimulus in hot plate test was longer
in LMEO-treated animals (150 mg/kg) (10.2 2.0 s.) than control group
(4.1 0,8 s.) at 30 minutes. Conclusions: LMEO presents
psychopharmacological effects suggestive of psycholeptics drug with
promising antinociceptive activity, indicating that this activity may probably
be mediated in the level of CNS.

Financial support from LTF, CNPq/Brazil.


245

CODE 227.2
INVESTIGATION OF ACUTE TOXICITY AND MIORRELAXANT EFFECT
OF Lippia microphylla (VERBENACEAE) ESSENTIAL OIL IN MICE

Penha, A. R. S
1
; Mota, V. G.
1
; Monte, L. O.
1
; Carvalho, F. L.
1
; Tavares, J.
F.
1
1
; Alencar, J. L.
2
; Almeida, R. N.
1

1
Laboratory of Pharmaceutical Technology, Center of Health Sciences,
Federal University of Paraiba (UFPB).
2
Department of Internal Medicine, Federal University of Paraiba (UFPB).
Corresponding author: vanvan_mota@hotmail.com

Introduction: The genus Lippia has yielded a great number of medicinal
and economically important species that are frequently used in folk
medicine. Psychopharmacological effects, such as sedative and
myorelaxant, have already been characterized for some species
3,4
.
Objectives: This study investigated the acute toxicity and the myorelaxant
effect of the Lippia microphylla essential oil (LMEO) in animal models.
Matherial and Methods: Swiss male mice (30-40g, 3 months old) were
divided into groups (n=8-10 animals) and treated intraperitoneally (i.p.) to
perform: 1) LD
50
1
: 100, 200, 300, 400, 500, 1000 mg/kg LMEO (used as
reference for the choice of relatively safe doses for the subsequent tests)
and 2) Rota rod test
2
: vehicle, 50, 100 and 150 mg/kg of the LMEO and
diazepam (2 mg/kg). All experimental protocols were approved by the local
Ethics Committee for Animal Research (CEPA N: 0304/08). Results and
Discussion: The LMEO lethal dose 50% was calculated by nonlinear
regression and this was estimated at 385.3 mg/kg with confidence limits
from 298.2 to 497.8 mg/kg. Thirty minutes after treatment with LMEO,
animals treated with a dose of 150 mg/kg showed a significant decrease in
time spent in the revolving bar at rota rod test (113.8 19.8 seconds
versus 179.1 0.8 seconds of control group). The other tested doses did
not promote significant changes in this parameter. Conclusions: These
results show that LMEO present low DL
50
, which may be an indicative of
toxicity, but it did not affect muscular coordination in mice, except in the
first minutes after treatment with higher doses.



246

CODE 228
CHARACTERIZATION OF THE OPUNTIA FICUS-INDICA (L.) MILL
DRIED EXTRACT BY THERMAL ANALYSIS AND INFRARED
SPECTROSCOPY

Souza, C. M. P.
1,2
; Soares, B. S. A.
2
; Santos, D. P. G.
2
; Polinario, A. C.
2
;
Silva, J. A.
3
; Freitas Neto, J. L.
1
; Leite, R. S.
1
; Santana, D. P.
1

1
Universidade Federal de Pernambuco (UFPE).

2
Grupo de Pesquisa Sistemas de Liberao de Frmacos e Biofarmcia,
3
Corresponding author: cinthyampsouza@hotmail.com

Introduction: The technique of spray drying has been widely applied in
obtaining dry extracts with better technology characteristics and greater
concentration of constituents with biological activities important for the
pharmaceutical area. Objectives: To characterize physico-chemically the
dried extract of Opuntia ficus-indica (L.) Miller, using thermoanalytical
techniques and infrared spectroscopy (IR). Materials and Methods: The
hydroalcoholic extract of cladodes of Opuntia ficus-indica was used to
obtain the nebulized extract in a Spray Dryer Lab Plant SD-05. The drying
temperature was 160C and used up 20% of aerosil. This dried extract
was thermally characterized by DSC, TG and DTA, using a sample of 2 mg
and a heating rate of 10C/min in thermoanalyticals equipments
(Schimadzu). It also applies to the IV spectroscopy (Perkin Elmer
Spectrum 400). Results and Discussion: The thermograms observed
indicate that there is no defined stages of transition from matter (DSC),
thermal degradation (TG) and physical transformations (DTA). This is due
to the complexity of the sample, which is a plant extract. The IR spectrum
showed absorptions in the region of 3200cm
-1
and 1650cm
-1
suggesting
the presence respectively of hydroxyl and carbonyl. The strong absorption
in the region of 1100cm
-1
(ethers), suggests the presence of a large class
of secondary metabolites, including flavonoids. Conclusions: The
thermoanalytical techniques and IR spectroscopy allowed to characterize
the dry extract of Opuntia ficus-indica, for subsequent use of this plant
material in the pharmaceutical area.

Financial support from Capes and CNPq


247

CODE 229
EXPRESSION STUDY OF THE GENES INVOLVED ON FOLIC ACID
METABOLISM IN ASSOCIATION WITH ORAL CLEFT DEVOLOPMENT

Soares, C. D.
1
; Oliveira, G. H. M.
1
; Cardoso, M. L.
1
; Bezerra, J. F.
1
; Souza,
Santos, F. M
1
. K. S.
1
; Morais, L. V. F.
1
; Oliveira; S. R.
1
; Farjado, C. M.
2
;
Silbiger, V. N.
1
; Luchessi, A. D.
2
; Hirata, R. D. C.
2
; Almeida, M. G
1
;
Rezende, A. A.
1
; Hirata, M. H.
2

1
2
Molecular Biology Laboratory, Toxical and Clinical Analisys Departament
,
State University of Sao Paulo.

Introduction: There is considerable evidence suggesting that folate-
related genes play a role in the etiology of oral facial clefts. Clefts are
known to have a strong genetic component. The expression profile of
genes involved on the pathway and folic acid metabolism which is linked to
oral clefts requires appropriate environmental causes in combination with
genetic factors that culminate in the failure of fusion of facial processes.
Objective: The aim of the present study was to analyze the gene
expression profile of MTHFR, MTR, MTRR, and RFC1, in association with
the occurrence of oral clefts. Materials and Methods: Were studied 80
subjects, 40 nonsyndromic oral cleft individuals, and 40 control subjects
without oral cleft. Serum biochemical measurements were performed of
folic acid, vitamin B12 and homocysteine. A questionnaire was given to
mothers about the use of cigarettes, alcohol and family history. Total RNA
was extracted from peripheral blood leukocytes. We perform the qPCR
with applied inventoried gene expression assays. Results and
Discussion: Individuals with oral cleft had reduced serum folic acid. The
alcohol use was reported as significant by mothers of affected individuals.
Syndromic oral cleft individuals had a significant reduction in the
expression of mRNA for the genes of MTR, p = 0.013, and MTRR, p =
0.003. The same result was not observed in MTHFR and RFC1 genes, p =
0.143 and 0.490, respectively. Conclusions: The significant reduction in
mRNA expression of enzymes MTR and MTRR in children with oral clefts
associated with the reduction of folic acid and alcohol use by mothers,
suggests that these patients have a genetic predisposition and the
environmental factors influence on the appearance of malformations such
as oral clefts.

Financial support from FAPESP/Brazil and master scholarship from
CNPq/Brazil.


248

CODE 230
PARAMETERS KINETICS BY OZAWA MODEL FOR NICOTINAMIDE BY
DYNAMIC TG

Souza, F. C. de
1
; Silva, E. P. da
1
; Almeida, V. D. de
1
; Azevedo, W. M. de
1
;
Arago, C. F. Soares
1
; Gomes, A. P. B.
1

1
Federal University of Rio Grande do Norte (UFRN)

Introduction: Thermal analysis methods are well-established techniques
in research laboratories of pharmaceutical industry, and thermogravimetry
(TG) has also been used to elucidate the kinetics of decomposition
reactions. Arrhenius equation and model Ozawa have been used to
calculate energy of activation and order of reaction from thermogravimetric
and volumetric curves. Applies the method of Ozawa for dynamic TG
curves and Arrhenius equation for the isotherms. Objective: Comparative
study between the use of the method of Ozawa and Arrhenius equation for
dynamic thermogravimetric curves of nicotinamide. Materials and
Methods: Nicotinamide raw material and capsules were obtained from
Farmafrmula Manipulation Pharmacy. It was weighted 10.50.5 mg in
alumina crucible. For kinetic determination, dynamic TG curves were
obtained using a Shimadzu thermobalance, model DTG60, under a
nitrogen flow at 50 mL.min
1
and heating rates of 10, 20, 40, 60 and
80C.min
1
up to 900C (n=3). The following kinetic parameters by Ozawa
model kinetic energy, order and frequency factor. Results and
Discussion: The nicotinamide showed the following kinetic parameters:
7

min-1) fo 0.1, as the nicotinamide capsules presented the following
kinetic parameters: order(0.5), kinetic energy (63.93 KJ/mol) and frequency
factor(2.93 x 10
6
min-1) fo 0.1. Conclusions: Nicotinamide capsules
have a lower thermal stability than the raw material due to nicotinamide
raw material has lower activation energy, which shows that there may be
some interaction in the formulation.

FAPERN/Brazil.


249

CODE 231
DEVELOPMENT AND VALIDATION OF A STABILITY INDICATIVE
METHOD OF ASCORBIC ACID (VIT C) FOR 500 MG TABLETS

Oliveira, A. R.2,4; Santana, A. K. M.1; Santos, B. A. dos1; Medeiros, F. P.
M. de1; Silva, M. J. da1; Dias, M. E. de S.3

1
Arraes (LAFEPE).
2
3
Universidade Federal de Pernambuco (UFPE).
Corresponding author: arsenio3000@hotmail.com

Introduction: Official compendia do not comprise a method for the
concentration quantification of active pharmaceutical input (API) Vit C by
HPLC, which allows evaluating related substances and potential synthesis
impurities.Objective: Develop an indicative analytical methodology for
stability study of ascorbic acid. Materials and Methods: The degradation
studies were conducted with API, utilizing H2O and solutions of HCl 1,0M,
NaOH 1,0M and H2O2 at 3,0%, at temperature (between 15 and 30C),
protected from light, during 15 days. The sample-solutions were diluted
with the degradation solution. At each analysis time, an aliquot was diluted
to final concentration of 500 ppm, with mobile phase
acetonitrile:ammonium acetate buffer. The developed quantification
method by HPLC was validated to the API and co-validated to the Vit C
500 mg tablet according to the RE 899/2003 ANVISA. Results and
Discussion: When submitted to HCl the active degraded 99,3% in 15
days. With NaOH was of 93,16% in 24 hours of exposition. In H2O2 did not
present a peak of the active at time zero, that is, it was degraded
immediately. With H2O, the reduction after 15 days was of 46,73%. The
utilized method was evaluated, showing to be linear, selective, precise,
exact and robust. The method for the concentration quantification of Vit C
in tablet was co-validated, thus presented linear, specific and precise.
Conclusion: The forced degradation study indicated a new method to Vit
C quantification by HPLC. The utilized method has been sensitive to
detection of possible degradation products, it was selected as an indicative
method of stability.


250

CODE 232
DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD
FOR QUANTIFICATION OF SIMVASTATIN IN MICROEMULSION BY
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Ferreira, L. F.
1
; Silveira, W. L. L.
1
; Xavier Jnior, F. H.
1
; Damasceno, B. P.
G. L.
2
; Egito, E. S. T.
1

1
2

Introduction: Unreliable analytical data can lead to incorrect decisions and
irreparable financial damage. Validation is a method that ensures the
specificity, accuracy and precision of an analytical test and thus ensures
reliable and reproducible results of a sample. Objectives: To develop and
validate an analytical method for quantification of simvastatin (SIV) in
microemulsion (ME) by high performance liquid chromatography (HPLC),
in accordance with the regulation 899/2003 of the National Sanitary
Surveillance Agency. Materials and Methods: The HPLC column was a
BDS Hypersil C18, measuring 150 x 4.6 mm i.d and the mobile phase
consisting of mixture acetonitrile and methanol (90:10, v/v), at a flow rate of
0.9 mL min
-1
. Analytes were measured by a UV detector set at 238 nm.
The calibration curve was obtained from solutions of SIV in acetonitrile at
predefined concentrations. The SIV was incorporated at a concentration of
10 mg.mL
-1
into the ME. The samples SIV-ME were subsequently diluted in
acetonitrile for the test assay. Results and Discussion: After the
chromatografphic analysis, the results obtained for precision
measurements (coefficient of variation) did not exceed 5%. The average
percentage recovery was between 98% and 102%. The coefficient of
regression linear (R
2
) and the values of limit of detection and quantitation
obtained proved the linearity and sensitivity of the method, respectively.
Conclusions: The results revealed that the method was specific, precise,
accurate, robust and linear in the range from 4 to 18 g.mL
-1
. Therefore, it
can be safely used to quantitation of SIV in microemulsion.

Financial Support from CNPq/Brazil.


251

CODE 232.2
RETROSPECTIVE AND CONCURRENT VALIDATION OF THE
MANUFACTURING PROCESS OF ZIDOVUDINE 300MG + LAMIVUDINE
RETROSPECTIVE AND CONCURRENT VALIDATION OF THE
MANUFACTURING PROCESS OF ZIDOVUDINE 300MG + LAMIVUDINE
150MG

Oliveira, A. R.
2,4
; Magalhes, L. C. de
3
; Oliveira, A. D. P. de
1
; Malheiro, J.
P. T.
2
; Monteiro, D. B.
1
; Granjeiro Jr, S
1
; Vasconcelos, F. V. C. de
1
; Arajo,
E. S. de
1

1
Arraes (LAFEPE).
2
3
Corresponding author: arsenio3000@hotmail.com

INTRODUCTION: In the pharmaceutical industry, validation is a practice
directly related to Good Manufacturing Practices (GMP), and a component
of quality assurance associated with a particular product or process. The
Statistical Process Control is a tool whose main purpose optimize
production, bringing higher quality products and cost reduction.
OBJECTIVE: Validate the manufacturing process of Zidovudine (AZT)
300mg + Lamivudine (3TC) 150mg. MATERIALS AND METHODS: The
study was conducted on the production line of the antiretroviral of the
LAFEPE. First, the records using batch produced in 2010, were developed
control letters and histograms, using the software WinCEP plus 5, with
data on weight, friability, hardness, disintegration, content and uniformity of
dosage units. The second part consisted of a concurrent validation of all
steps of the production process of thee lots, as is recommended in the
RDC 17/2010 ANVISA, and compared against the limits established in
the retrospective validation. RESULTS AND DISCUSSION: The
retrospective validation process characterized as capable in all
parameters. The results presented for samples collected in the production
process were mostly within the limits established in the letters controls,
minor deviations in the uniformity and content of some samples, but not
running away from the specifications laid down by law. CONCLUSIONS:
The production process of AZT + 3TC met the requirements of GMP, and it
can be said that the drug provides security in the final product. The
statistical analysis of historical data proved to be important because the
values were significantly validation alert, which diverted the limits, even
meeting the specifications.


252

CODE 233
A STUDY OF CYTOTOXICITY BY BIOASSAY TECHNIQUES OF THE
HOMEOPATHIC PRODUCTS CONTAINING Momordica charantia

Espsito, R. C.
1
; Andrade, M. G. M.
1
; Micali, I. A.
3
; Navarro, M. V. M.
3
;
Rocha, L. G.
2
; Arago, C. F. S.
1

1
Quality Control of Drugs Laboratory, Department of Pharmacy, Federal
2
Laboratory of Antiparasitic Testing, Department of Microbiology and
Parasitology, Federal University of Rio Grande do Norte (UFRN).
3
Laboratory

of Homeopathic Pharmaceuticals, Department of Pharmacy,

Corresponding author: rcesposito@yahoo.com.br

Introduction: The quality control of homeopathic preparations using
common analytical techniques is challenging because its characteristic of
high dilution. Biological assays in vitro have been shown positive results to
evaluate the mechanism of action of homeopathics, able to act on a
specific system such as homeopathic equalizers (BELLAVITE et al 2006).
Objective: This study aims evaluated the quality of homeopathic products
containing Momordica charantia leaf and fruit of 3DH and 6CH with
biological assay in vitro. Materials and Methods: The cytotoxic activity of
homeopathic products containing Momordica charantia leaf and fruit of
3DH and 6CH potencies have been dinamized with milli-q water and their
mother tinctures (TM) according to the Brazilian Homeopathic
Pharmacopoeia (FHB) against procyclic promastigotes cells of Leishmania
(L) chagasi. The samples (n=5) were prepared and incubated for 24 hours
in a Qualxtron biological camera at 27C and the viable cells was
quantified in a Neubauer chamber through the optical microscope. Results
and Discussion: Mother tinctures showed cytotoxicity to cells during
incubation and TM leaf showed higher cytotoxic activity than homeopathic
products. The dynamized medicines 6CH showed more cytotoxicity than
3DH. Conclusions: These results indicate that homeopathy products
containing Momordica charantia have cytotoxic properties.

Financial support from CAPES/Brazil and PROPESQ-REUNI-
UFRN/Brazil.


253

CODE 234
UV-VIS ANALYSIS OF CHEMICAL MARKERS OF THE LEAVES OF
Passiflora edulis Sims VARIETY edulis

Soares, T. C; Siqueira, E. M. S.
1
; Reginatto, F. H.; Ramos, F. A.;
Castellanos, L.; Duque, C.; Schenkel, E. P.; Zucolotto, S. M.
1

1
Norte (UFRN).
Universidade Federal de Santa Catarina (UFSC), Florianpolis-SC, Brasil.
Departamento de Qumica, Universidad Nacional de Colombia (UNAL).

Introduction: Brazil has a large variety of species of Passiflora genus.
Known as maracujs, the species Passiflora edulis variety flavicarpa
Degener (yellow passion fruit/maracuj-amarelo) and Passiflora edulis
Sims variety edulis (purple passion fruit/maracuj-roxo) occur widely in the
South America and are popularly used in the treatment of anxiety,
migraine, nervousness and insomnia. The glycosilated flavonoids are the
main compounds related of these species. Objective: The aim of this
study was to isolate the main compounds of the leaf extract from Passiflora
edulis Sims variety edulis to differentiate the varieties of P.edulis.
Materials and Methods: The leaves of P. edulis Sims variety edulis were
collected in Colombians Andes. The aqueous extract of leaves was made
by infusion and partitioned with ethyl acetate (EtOAc) and n-butanol
(BuOH). The thin layer chromatography (TLC) analysis was performed
using different systems, NP reagent A as detector and observation on UV
254/365 nm. The BuOH fraction was submitted to many chromatographic
processes as flash column, chromatographic column using silica gel, and
Sephadex LH 20. These processes resulted in a purified fraction, that was
submitted to UV analysis. Results and discussion: The compound
showed a Rf 0.85 and a spot deep purple (UV 254 nm), but no developing
color after revelated with NP reagent A. In the UV spectrum was observed
UV maximum absorption at 261 nm (band II) and lacks the band I,
characteristic in the flavonoids Conclusion: These results showed that the
comparison between UV data of the literature and the characteristics
observed by TLC suggested that the compound present similarity UV
absorption of a chrysin.


254

CODE 236
ANTICONVULSIVANT AND ANXIOLITIC ACTION EVALUATION OF
INDIGOFERA SUFFRUTICOSA MILLS AND INDIGO CARMINE IN MICE.

Soares, P. H. S.
1
; Almeida, E. R.
2
; Chaves, T. M.
3
; Silva, A. R.
2
; Arago-
Neto, A. C.
2
; Couto, G. B. L.
2
; Luna, R. L. A.
2
, Silva, R. P.
2

1
Pernambuco (UFPE).
2
Departamento de Antibiticos, Universidade Federal de Pernambuco
(UFPE).
3
Departamento de Odontologia, Universidade Federal de Pernambuco
(UFPE).
Corresponding author: phdssoares@gmail.com

Introduction: The use of plants in he ced fok medcne s
characteristic of Brazilian cultural heritage. Among the numerous plants
popularly used Indigofera suffruticosa M s found. Known s ne nd
indigo field, is used in the treatment of renal and prostate diseases.
Objective: The present study had as an objective the evaluation of the
anticonvulsant and anxiolytic action of Indigofera suffruticosa Mill and
indigo carmine molecule. Materials and Methods: Swiss albino mice (Mus
musculus) aged between 45-60 days and weighing 22-32 g were used.
The mice had been submitted to doses of 100, 300 and 500mg/kg of the
methanol extract and 30 mg/kg of indigo carmine dissolved in saline
solution. To anticonvulsivant action evaluation, after one hour, the groups
of animals had suffered the reaction from following convulsivants,:
Pentylenetetrazole, Picrotoxin, Strycnine, Pilocarpine and Lidocaine. To
anxiolytic study, the mice had been submitted to the Plus-Maze and
Marble-Burying tests. As standard drug, Diazepam was used in all the
experimental models. Results and Discussion: The methanol extract
presented excellent anticonvulsant action, increasing the time of latency for
the beginning of the first convulsion in all the tests, an effect similar to that
presented by the indigo carmine. Conclusions: The results of this study
demonstrate that this is a promising plant for future studies in the
development of new anticonvulsants drugs, and indigo carmine as an
isolated molecule.


255

CODE 237
DEVELOPMENT OF NEW METHODS OF ANALYSIS AND CONTROL
OF METRONIDAZOLE TABLETS USING NEAR INFRARED

AZEVEDO, L. F.
1
*; Dias, S. B. T.
1
; Rocha, V. N.
2
; Frana, D. M. M.
2
; Brito,
K. G. S.
1
; Silva, E. L.
1
; Nascimento, T. G.
1

1
Laboratrio de Anlises e Controle de qualidade de Frmacos e
Medicamentos, Grupo de Pesquisa em Tecnologia e Controle de
Qualidade de Medicamentos,Curso de Farmcia, Escola de Enfermagem e
Farmcia, Universidade Federal de Alagoas(ESENFAR/UFAL) Av.
Lourival Melo Motta, s/n, Campus A.C. Simes, Tabuleiro dos Martins,
Macei Alagoas- CEP: 57072-970.
2
Laboratrio Industrial Farmacutico do Estado de Alagoas, Setor de
Control de Qualidade de Medicamentos(LIFAL)
Corresponding author: fariaslais@hotmail.com

Introduction: The development of new methods of quality control with
low-cost and reproducible for drugs, bulks and medicines are essential to
establish new criteria and quality specifications. Assays of medicines using
near-infrared (NIR) can be a viable alternative in the development stage of
new products LIFAL. Objective: The work aimed to evaluate the
compatibility of metronidazole and excipients used in the manufacture of
tablets and identify possible excipients used in a standardized composition
of metronidazole using the near infrared technique. Materials and
Methods: The compatibility study was conducted based on the analysis of
binary mixtures, which consisting in physical mixtures of metronidazole
(active ingredient) and pharmaceutical excipients (lactose, cellulose MC
101, talc, magnesium stearate and aerosil) in pre-determined proportions.
The raw materials, binary mixtures and standardized tablets of
metronidazole were subjected to analysis of near-infrared. Results and
Discussion: Using NIR analysis, it observed that binary mixtures
metronidazole-excipients do not present pharmacotechnical
incompatibilities, because the NIR bands of the raw material of
metronidazole are maintained. Comparison of infrared spectrum of binary
mixtures and the standardized tablet of metronidazole revealed the
appearance of stretch at 4750cm-1, 5150 cm-1and 6850 cm-1 which are
derived from cellulose MC 101 without possibility of these bands, mainly
(5150 cm-1), to occur in the lactose excipient. Conclusion: The infrared
spectrum was very useful to analyze the compatibility of excipients in
formulations of metronidazole tablets, besides to provide technological
information to detect the presence of specific composition in standardized
formulation of metronidazole.



256

CODE 238.1
CARACTERIZATION OF RIFAMPICIN AND THEIR DEGRADATION
PRODUCTS BY CHEMICAL STRESS

Amorim, M.V.P.; Assis, W.R.S; Costa, A. F. O; Arajo, A. A; Arago,
C.F.S.

Laboratrio de Controle de Qualidade, Departamento de Farmcia,
Laboratrio de Farmacologia, Departamento de Farmacologia,
e-mail: marcelo@nuplam.ufrn.br

Introduction: The drugs have been more and more researched. According
to ANVISA, the procedures have to present identification and quantification
results of degradation products, followed by analytical method
correspondent. For this, the procedures have to present initial studies
using the drugs in some stress conditions. Rifampicin is one of the main
drugs chose to treat the tuberculosis, an illness considered negligible in
many countries. Objective: The objective was to characterize the
Rifampicin and its degradation products after a chemical stress. Materials
and Methods: A chemical stress was carried out using 0.1N HCl and 0.1N
NaCl. Furthermore, they were analyzed using a C8 (125mm x 4.6mm)
column at a flow rate of 1.6 mL.min
-1
, with a run time of 15 min at 254nm.
The mobile phase was a mixture of phosphate buffer, water, acetonitrile,
1.0 M citric acid and 0.5 M sodium perchlorate (100:350:510:20:20 v/v).
Results and Discussion:Two samples were prepared consisting of
Rifampicin and their degradation products, separately, containing about 0.2
mg of each per mL in a mixture of water and acetonitrile (50:50 v/v).
Injections of equal volumes (50 L) of both solutions were inserted at the
chromatograph and the peaks could be compared. Both chemical stress
(acid and basic) were successful, due a complete degradation, once
compared to the solution containing Rifampicin. Also, the visual aspect of
the both degradation products solutions and the Rifampicin were different.
Conclusion:Further studies will be conducted in order to carry out others
stress tests, as photolytic, heat, moisture and oxidative.

Financial Support from Master scholarship from CAPES/Brazil.


257

CODE 238.2
COMPARISON STUDY OF TWO ANALYTICALS METHODS OF 4-FDC
USING A SINGLE COLUMN

Amorim, M.V.P.; Costa, A. F. O; Arajo, A. A; Arago, C.F.S.

Laboratrio de Controle de Qualidade, Departamento de Farmcia,
Laboratrio de Farmacologia, Departamento de Farmacologia,
Corresponding author: marcelo@nuplam.ufrn.br

Introduction: Tuberculosis is one of the illnesses most dangerous, but can
be curable in practically 100% of new cases, since the principles of modern
chemotherapy are obeyed. The drugs considered first class in the
treatment are: Isoniazid, Rifampicin, Pirazinamide and Ethambutol (4-Fixed
Dose Combination or FDC). Objective: The aim is a comparison of two
screenings involving acetonitrile and methanol to obtain the 4-FDC in a
single column. Materials and Methods:Two screenings were done with a
C18 (250 mm x 4.6 mm) column at a flow rate of 1,5 mL.min
-1
with a run
time of 60 min and DAD range 200 800 nm. The buffer solution
(ammonium acetate and copper II acetate monohydrate in water) was used
on the mobile phase with acetonitrile in the first screening and with
methanol in the second. A sample containing the 4 drugs and 4 samples
containing the drugs separately were prepared into volumetric flasks. Both
screenings obtained the 4 drugs into the same analyze. Results and
Discussion: The retention time with acetonitrile presented 7.34, 7.91,
10.59 and 32.30 min, while methanol 8.11, 8.98, 14.33, 43.86 min of
Isoniazid, Pirazinamide, Ethambutol and Rifampicin, respectively.
However, the screening with acetonitrile showed system suitability (tailing
factors, theoretical plates, resolutions and widths) better than that
consisted of methanol. Many wavelengths were analyzed and the best was
270 nm. Conclusion: Further studies will be conducted in order to improve
this analytical method.

Financial Support from Master scholarship from CAPES/Brazil.


258

CODE 239
EVALUATION OF ANALYTICAL METHODS APPLIED TO
QUANTIFICATION OF LIPID PEROXIDATION IN LIVER
HOMOGENATE OF RATS

Morais, L.
1
, Arajo-Silva, G.
1
, Solon, L.G.S.
2
, Almeida, MG.
1

1
LABMULT, Department of Clinical and Toxicological Analyses -
UFRN/Natal-RN/Brazil
2
Quality Control of Drugs Laboratory, Department of Pharmacy-
UFRN/Natal-RN/Brazil;

Corresponding author: leandro_moraisvinicius@hotmail.com

Introduction: The lipid peroxidation (LP) is one of the main results of
oxidative stress. It occurs in polyunsaturated fat acids, starting with the
dc OH, leading to formation of alcooxil and peroxyl radicals. The
malondialdehyde (MDA), which is the most abundant product in LP,
induces structural modifications in biological molecules. This aldehyde is
the main marker of LP. It is commonly evaluated by spectrophotometry
through reaction with Thiobarbituric Acid (TBA) thus due to numerous
interfering it is evaluate as reactive substances of this chromogenic
reagent (TBARS). Objective: The purpose of this work was to set a
comparison between analytical methods enshrined in the scientific
literature about determination of lipid peroxidation marks in liver
homogenate of rats (control and intoxicated groups) by CCl4. Materials
and Methods: At first was used reaction TBARs, N-metil-2-fenilindol
(MDA-MFL) reaction, and quantificacion of complex TBA-MDA by HPLC-
DAD. Results: In control animals group the parameters of lipid
peroxidation (TBARS = 23,72 1,4 mol/L ; MDA-MFL = 19,63 2,6
mol/L) were decreased in comparison with intoxicated animals by CCl4
(TBARS = 78,83 12,4 mol/L ; MDA-MFL = 65,54 8,6 mol/L ). The
results MFL-MDA were lower than TBARS in the same group, suggesting
absence of interfering. Conclusion: The comparison of TBARS and MDA-
MFL methods showed that the second one was more specific than the first
one. Other results will be obtained by HPLC-DAD analysis, also for
comparison, due its specific methodology for quantification of MDA.

Finnantial supported from CNPq/Brazil.


259

CODE 240
VALIDATION OF AN UV SPECTROPHOTOMETRIC METHOD FOR
DETERMINATION OF PROPRANOLOL IN CAPSULES USING
QUITOSAN AS EXCIPIENT

Fonseca, G. D.
1,
*; Medeiros, A. S. A.
1
; Fernandes-Pedrosa, M. F.
1
; Silva
Junior, A. A.
1
; Santos, K. S. C. R.
1

1
Laboratrio de Tecnologia e Biotecnologia Farmacutica (TecBioFar),
Departamento de Farmcia (DFAR),Universidade Federal do Rio Grande
do Norte (UFRN).
Corresponding author *gabrieladiniz17@hotmail.com

Introduction: Chitosan is a linear polysaccharide composed by
copolymers of glucosamine and N-acetyl glucosamine obtained from partial
deacetylation of chitin. Objective: To study chitosan as excipient in solid
dosage forms, propranolol was used as probe drug to evaluate the
chitosan performance as diluent and disintegrant in capsules. Materials
and Methods: A UV spectrophotometric method for determination of
propranolol in capsules containing quitosan was validated. The parameters
specificity, linearity, precision and accuracy were studied according to
validation guidelines. Results and Discussion: The method showed
specificity and linearity in the concentration range of 32 to 48 mg.mL
-1
,
presenting a good correlation coefficient (r = 0,9994). The intra and
interday precision data demonstrated the method has good reproducibility
(relative standard deviation < 2,0 %). The accuracy determined from
recovery test presented satisfactory recoveries percentual of propranolol
(103,4 - 109,4 %), which indicates there was no interference of the
excipients in the determination of the active pharmaceutical ingredient.
Conclusions:These results show that the method can be applied to assay
propranolol in capsules using chitosan as excipient.

Financial support from CNPq and FAPERN/Brazil


260

CODE 241
CHROMATOGRAPHIC PROFILE OF THE CRUDE EXTRACT AND
FRACTIONS FROM K. brasiliensis Cambess (SAIO) LEAVES

Fernandes, J. M.; Negreiros Neto, T. C.; Oliveira, A. C.; Arajo-Silva,
G.; Morais, L. V. F.; Soares, L. A. L; Zucolotto, S. M.

Federal do Rio Grande do Norte, Natal, RN, Brasil.
LABMULT, Departamento de Farmcia, Universidade Federal do Rio
Grande do Norte, Natal, RN, Brasil.
Departamento de Farmcia, Universidade Federal de Pernambuco,
Recife, PE, Brasil.
Corresponding author: jumorais07@yahoo.com.br

Introduction: The specie Kalanchoe brasiliensis Cambess,
(Crassulaceae), known popularly as saio, is widely used in folk medicine
as anti-inflammatory and healing. There are not works about the
chromatographic profile of the chemical markers of this species.
Objectives: The aim of this study was to evaluate the chromatographic
profile of the crude extract and fractions from the leaves of K. brasiliensis
by High Performance Liquid Chromatography (HPLC) with Diode Array
Detector (DAD) to characterize the chemical markers of this species.
Materials and Methods: The hydroethanolic extract (HE) 50% was
obtained by turboextraction from fresh leaves. The HE was submitted a
partition, resulting in the CHCl
2
and AcOEt fractions. The HPLC analysis
was performed using a Lichrospher RP 18-5 column, in a gradient system-
water:formic acid (99.7:0.3, v/v) and MeOH:formic acid (99.7:0.3, v/v), flow
(0.8 mL/min) and UV detector (254 and 370 nm). Results and
Discussion: The chromatogram of the HE showed six main peaks. All
peaks had similar UV spectrum with three maximum absorption (259 nm
and 271 nm- band II and 355 nm- band I), characteristic of the flavonoids
nucleous. The chromatogram obtained of the AcOEt showed six main
peaks, with had similar UV spectrum with two maximum absorption at 262
nm (band II) and 358 nm (band I). The chromatogram of CH
2
Cl
2
fraction
showed five main peaks with two different spectrum profiles (1. 262 nm-
band II and 358 nm- band I; 2. 232 and 268 nm- band II and 324 and 362
nm- band I). According with literature data, suggests that the main
compounds of the HE are patuletin-3-O-glycoside derivatives.
Conclusions: This method showed a chromatographic profile satisfactory,
with good resolution and symmetrical peaks. The fraction AcOEt fraction
showed the main compounds of the HE. Further studies are been
conducted to characterize these patuletin-3-O-glycoside derivatives.


261

CODE 242.1
KINETCS STUDIES OF Euterpe oleracea (AA) EXTRACTS

Daher, C. C.
1
; Rodrigues, R. O.
2
; Fontes, I. S.
2
; Soares, D. S.
2
; Ferrari,
M.
2
; Arago, C. F. S.
3
. Gomes, A. P. B.
3

1
2
3
Laboratory of Quality Control Faculty of Pharmacy, Federal University
of Rio Grande do Norte.
Corresponding author: daher@unp.br

Introduction: Euterpe oleracea is an Amazon palm tree. Their extract can
be used in cosmetics preparations. The thermals methods are used to
characterize actives and excipients and kinetics studies. Objectives: The
aim of this work was to evaluate and compare the kinetics behavior of
glycolic extract and dried extract as raw materials. Materials and
Methods: The TG curves were obtained with a Shimadzu thermobalance
(DTG-60), under a N
2
flow 50mL.min
-1
, at heating rate 10
o
C.min
-1
, up to
temperature of 900C. Sample mass about 10mg were packed in an
alumina cell. For the decomposition, the activation energy (Ea), the order
of reaction (n) and the frequency factor (Z) were obtained from first step of
TG curves. The curves obtained were analyzed for software Shimadzu
TA60 TASYS. The kinetic parameters were calculated with o values of
0.10.9 by utilizing the mathematical equations of Coats-Redfern (CR),
Madhusudanan (MD), Horowitz-Metzger (HM), and Van-Krevelen (VK).
The kinetic data were calculated by four mathematics models. Results and
Discussion: Presents the values of the order of reaction (n), the activation
energy (Ea) and the frequency factor (Z) for glycolic extract and dried
extract, with the best linear correlation coefficients (r). Glycolic extract (n /
Ea - kJ.mol
-1
/ Z/s
-1
): CR (1.57/56.98/4.29x10
6
); MD (1.61/57.93/6.77x10
6
);
HM (1.91/71.77/1.03x10
9
); VK (1.79/ 64.73/9.48x10
12
); Dried extract: CR
(4.82/73.73/6.52x10
9
); MD (4.62/72.15/3.64x10
9
); HM
(4.88/99.26/6.04x10
12
); VK (4.80/85.95/7.31x10
16
). Conclusions: It was
found that the integral methods (CR and MD) and approximation methods
(HM and VK) yielded kinetic data that were reasonably comparable with
each other.

from UFRN/Brazil.


262

CODE 242.2
SUNSCREENS O/W EMULSIONS STABILIZED BY RHEOLOGICAL
MODIFIERS: PRELIMINARY STABILITY TESTS

Daher, C. C.
1
; Fontes, I. S.
2
; Rodrigues, R. O.
2
; Soares, D. S.
2
; Ferrari,
M.
2
.

1
2
Corresponding author: daher@unp.br

Introduction: The emulsions are systems thermodynamically instable. The
effect of some additives to increase the viscosity can be used to improve
the stability. Objective: The aims of this work were to development and
evaluate the preliminary stability of sunscreens formulations containing
rheological modifier. Materials and Methods: Emulsions were prepared
with Acrylates/C10-30 Alkyl Acrylates Crosspolymer (AAAC) (0.3%). To
improve the stability the different concentration of Polyacrylamide and C13-
14 Isoparaffin and Laureth 7 (PCIL) (0.5% PS1, 1.0% PS2 and 1.5% PS3)
were used. The centrifugation test, freezing-thawing cycle (FTC) and
thermal stress (TS) were used to investigative the preliminary stability
tests. The formulations were assessed through stability was assessed
through the organoleptic characteristics, pH and electrical conductivity
values, macroscopic analysis and rheological behavior determinations.
Statistical analyses were performed (p<0.05). Results and Discussion:
The O/W emulsions were prepared. The samples did not show any
changes in aspect after the centrifugation test. The PS1 was unstable in
the preliminary stability tests. The PS3 formulation showed statistical
differences in the electrical conductivity values after TS and FTC tests. An
improvement in the stability was shown when the emulsion was formulated
with PCIL (1.0% - PS2). Rheological measurements showed that these
emulsions exhibited non-Newtonian pseudoplastic flow behavior.
Conclusions: O/W emulsions containing 0.3% of AAAC and PCIL (1.0%)
have been found to be preliminary stables and should be conducted to
accelerated stability tests. The authors thank Basf, Chemyunion, Croda,
ISP and Mapric for the gift of sample materials.

from UFRN/Brazil.


263

CODE 243
STUDY BY QUANTUM MECHANICAL OF INTERACTIONS BETWEEN
AMPA RECEPTORS AND FLUORINE WILLARDIINE

Lima Neto, J. X.
1
, Ourique, G. S.
2
,

Oliveira, J. I. N.
1
, Albuquerque, E.L.
1
,
Freire, V. N.
3
, Fulco, U. L.
1

1
Department of Biophysics e Pharmacology (DBF), Federal University of
Rio Grande North (UFRN).
2
Department of Biochemistry (DBQ), Federal
University of Rio Grande North (UFRN).
3
Department of Physics, Federal
Corresponding author: neto.xavier01@gmail.com

Introduction: AMPA receptors are essentials in the development and
homeostasis of in the nervous system, the deficiencies in normal functions
of that kind of receptors are the major causes of damages in CNS.
However, the responsible mechanism for this function aren't completely
well knowledge. Objective: To elucidate this question, four willardiine
partials agonists were obtained with fluorine willardiine (FW) if the major
efficiency. Recent researches have indicated the QM methods like an
effective way in the achievement of the receptor-ligand interaction values.
In this work, we aim, the achievement of the AMPA-FW interaction energy,
with the major importance residues. Materials and Methods: Using the
crystallographic dates and the QM techniques named Molecular
Fractionation with Conjugate Caps (MFCC), based in the proteic system
fractionation, in which all of this amino acids fragment passed by quantum
treatment. Results and Discussion: By the complexed crystallographic
structure of the GluA2-FW we identified the amino acids Glu705 and
Arg485 with the larger attraction (-68 kcal/mol and -50 kcal/mol) and
Lys656 and Lys449 (10 and 12 kcal/mol) with the major repulsion. The
residues Thr655, Leu650, Thr480, Leu479 were showed as important
residues responsible for the maintenance of the receptor functions, the
total energy of the system is -330 kcal/mol. Conclusions: With the
obtainment of this dates, is possible a detailed understanding of AMPA and
the creation of the new drugs against neurodegenerative diseases.



264

CODE 244

EVALUATION OF THE ANTI-INFLAMMATORY POTENTIAL
ETHANOLIC EXTRACT FROM VERNONIA POLYANTHES

Temponi, V.S.
1
; Silva, J. B.
1
; Fernandes, F. V.
1
; Alves, G. A. D
1
; Matos, D.
M.
1
; Gasparetto, C. M.
1
; Pinho, J. J. R. G., Ribeiro, A.
2
; Alves, M. S.
1
;
Sousa, O. V.
1

1
Departamento de Cincias Farmacuticas, Faculdade de Farmcia,
Universidade Federal de Juiz de Fora
2
Departamento de Bioqumica, Instituto de Cincias Biolgicas,
Corresponding author: vanessatemponi@hotmail.com

Introduction: Vernonia polyanthes, found in South America, is traditionally
used as anti-rheumatic, healing and anti-inflammatory. Objective: The aim
of this study was to investigate the anti-inflammatory activity of ethanolic
extract from V. polyanthes. Materials and Methods: Dried and powdered
leaves were extracted with ethanol by static maceration. The anti-
inflammatory activity was assessed by paw edema and pleurisy methods.
Statistical significance was determined by analysis of variance and Student
Newman-Keuls test (p < 0.05). Results and Discussion: 1 and 2 h after
the application of carrageenan, the paw edema was reduced by 19.05 and
22.03% at a dose of 400 mg/kg, respectively. The inhibition of edema was
observed 3 and 4 h after carrageenan injection at the doses of 200 mg/kg
(16.41 and 27.87% respectively) and 400 mg/kg (25.78 and 31.97%). The
volume of exudate was reduced at the doses of 200 and 400 mg/kg in
13.83 and 43.08%, respectively. The total number of leukocytes was also
inhibited at the doses of 200 (17.87%) and 400 mg/kg (28.39%). Based on
these results, the anti-inflammatory effect of ethanol extract may be
mediated by inhibition of prostaglandin synthesis. Conclusion: the data
suggest that V. polyanthes can be an active source of substances with
anti-inflammatory activity, which justifies the popular use.

Financial support from CAPES/Brazil, FAPEMIG/Brazil and UFJF/Brazil.


265

CODE 245
EVALUATION OF THE ANTI-INFLAMMATORY POTENTIAL
ETHANOLIC EXTRACT FROM Vernonia condensata

Silva, J. B.
1
; Temponi, V. S.
1
; Fernandes, F. V.
1
; Alves, G. A. D
1
; Matos, D.
M.
1
; Gasparetto, C. M.
1
; Pinho, J. J. R. G.
1
; Ribeiro, A.
2
; Alves, M. S.
1
;
Sousa, O. V.
1

1
Departamento de Cincias Farmacuticas, Faculdade de Farmcia,
2
Departamento de Bioqumica, Instituto de Cincias Biolgicas,
Corresponding author: juceliabs@yahoo.com.br

Introduction: Vernonia condensata has been used in folk medicine as
analgesic agent. Objective: The present study investigated the anti-
inflammatory activity of ethanolic extract from V. condensata. Materials
and Methods: Dried and powdered leaves were extracted with ethanol by
macerating static. The anti-inflammatory activity was assessed by paw
edema and pleurisy in rats. Statistical significance was determined by
analysis of variance and Student Newman-Keuls test (p < 0.05). Results
and Discussion: The inhibition of edema was observed from 2 hours after
administration of carrageenan at doses of 200 and 400 mg/kg (0.46 0.04
and 0.45 0.07, respectively). 3 hours after injection, the doses of 100,
200 and 400 mg/kg reduced paw edema in 21.50, 22.58 and 32.26 %,
respectively. Doses of 100, 200 and 400 mg/kg (0.85 0.05, 0.70 0.09
and 0, 74 0.06) after 4 hours of application also produced inhibition of
edema in 22.72, 36.36 and 32.72%, respectively. The effects on pleurisy
showed that the doses of 200 and 400 mg/kg reduced the volume of
exudate (17.30 and 35.13%, respectively) and the inhibition of leukocyte
migration occurred at doses of 100, 200 and 400 mg/kg (10.18 0.24, 9.47
0.10 and 8.17 0.14, respectively). Based on these results, the anti-
inflammatory effect of ethanol extract from V. condensata leaves may be
mediated by inhibition of prostaglandin synthesis. Conclusion: this study
showed that the crude ethanolic extract of the plant has anti-inflammatory
activity, which justifies the popular use.

Financial support from CAPES/Brazil, FAPEMIG/Brazil and
UFJF/Brazil.


266

CODE 245
EVALUATION OF MUTAGENICITY AND ACUTE TOXICITY IN MICE OF
Spondias purpurea (Siriguela) ETHANOLIC EXTRACT.
Dantas, AM
1
; Coutinho, AC
1
; Luz, SMD
1
; Lima, MS
1
; Lira, WM
2
; Santos,
VL
1*
1
Laboratory of Pharmacological Assays, Department of Pharmacy, State
University of Paraiba (UEPB),
2
Laboratory of Genetics, Department of
Biological Sciences, State University of Paraiba (UEPB).
Corresponding author: vandals@ccbs.uepb.edu.br

Introduction: The Spondias species, belonging to Anacardiaceae family,
is the second most important gender in Brazil and many species are
scattered throughout the country, some with wide occurrence in the
Northeast. Many of these species are used as medicines. The species
Spondias purpurea, known as Siriguela, is employed against renal pain,
such as antidiarrheal, antispasmodic, diuretic, analgesic and antianemic.
Objective: Evaluate the acute toxicity of S. purpurea ethanolic extrat and
its potential mutagenic effects using the standard micronucleus. Materials
and Methods: To determine the toxicity were used adult mice of both
sexes who received through the oral route 2000 mg/kg of the extract and
they were observed after 30 min., 1, 2, 3 and 4 hours daily until 14 days. In
the model for mutagenicity evaluation of the extract was administered at
doses: 500, 1000 and 2000 mg / kg orally. 24 hours after treatment blood
was taken from tails of mice. Results and Discussion: Acute
administration of S. purpurea extract (2000mg/Kg) did not promote
behavioral changes, mortality, and no difference in body weight gain and
vital organs and no mutagenic effect was found in the micronucleus test in
peripheral blood cells of mice. The micronucleus test evaluates in vivo
chromosome damage in rodent hematopoietic cells. Conclusion: The
ethanolic extract, at the doses used, did not show acute toxicity nor
promote change or genetic damage or damage to the DNA repair
mechanism which would result in an altered cell.
Financial support from CNPq/UEPB/Brazil

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