The distribution and organization of 5S and 18S 5.

8S 26S (18S) rDNA were studied in 10 varieties of hexaploid Ipomoea batatas , five accessions of tetraploid Ipomeo a trifida , and six related species (five diploids, I. trifida, I. triloba, I. t iliacea, I. leucantha and I. setosa and one tetraploid, I. tabascana ), by using fluorescence in situ hybridization (FISH). A distribuio e organizao do 5S e 18S-5.8 S-26S (18S) de rDNA foram estudados em 10 variedades de Ipomoea batatas hexaplide , cinco acessos de trifida Ipomeoa tetraplides, e seis espcies afins (cinco diplide s, I. trifida, I. triloba, I. tiliacea, leucantha I. e I. setosa e uma tetraplide , tabascana I.), atravs de hibridizao fluorescente in situ (FISH). The FISH data ob tained indicated that polyploidization was followed by decrease in the number of 18S rDNA loci in higher ploidy level and provided evidence for major genomic re arrangements and/or diploidization in polyploid I. Os dados obtidos indicam que a FISH poliploidizao foi seguido por uma diminuio do nmero de loci de DNAr 18S em nvel de ploidia e forneceu provas de grandes rearranjos genmicos e / ou em diploidiza tion I. poliplides batatas . batatas. Among the five diploid species examined, I. Entre as cinco espcies diplides examinados, I. trifida appeared to be the most cl osely related to I. trifida parecia ser o mais estreitamente relacionado com I. batatas . batatas. By contrast, I. Em contrapartida, I. leucantha was closed to I. leucantha foi encerrada a I. tiliacea , but both species were distant from sw eet potato. I. tiliacea, mas ambas as espcies estavam distantes da batata-doce. I . triloba and I. triloba e I. setosa were distantly related to the rest of Ipomo ea batatas complex. setosa foram parente distante do resto da Ipomoea complexo. The close relationship between I. A estreita relao entre I. trifida and I. trifida e I. batatas was also demonstrated by the presence of one 18S and CMA marker in these two chromosome complements only. batatas tambm foi demonstrada pela presena de um marcador 18S e CMA nestes dois cromossomos apenas complementa. Based on c hromosome morphology, tetraploid I. Com base na morfologia cromossmica, tetraplide s I. trifida appeared to be more closely related to sweet potato than I. trifida parecia estar mais intimamente relacionado com a batata-doce do que eu tabascan a . tabascana. Taking all data obtained in this study, I. Tomando todos os dados obtidos neste estudo, I. trifida might be the progenitor of I. trifida pode ser o progenitor de I. batatas , and I. batatas, e I. tabascana , interspecific hyb rid between these two species. , Hbridos interespecficos tabascana entre estas dua s espcies.