Identificação do potencial bactericida

do extrato de Pterodon pubescens.

Integrantes;
 Kézia Hemily Sousa Brito
 Marcos da Silva Gabá
 Objetivos;
 Este trabalho tem como objetivo identificar,
isolar e avaliar o potencial bactericida do
extrato de Pterodon pubescens.
Obtenção dos extratos dos frutos e
sementes de Pterodon pubescens;
 Osfrutos de Pterodon pubescens passarão por
maceração em cadinho de porcelana e utilizando
como solvente o etanol 100% a extração será feita
por método de Soxhlet durante 8 horas. Todos os
extratos serão evaporados sob pressão reduzida,
embasados em vidro âmbar, conservados em
geladeira a 4±1 ºC e armazenados.
Padronização dos inóculos bacterianos;
 As culturas de micro-organismos serão mantidas a 4°C em
ágar nutriente (AN). As amostras serão recuperadas em caldo
Mueller-Hinton (MH) e incubadas sem agitação durante 24
horas a 36°C. Posteriormente, os inóculos serão replicados em
placas de ágar Mueller Hinton (AMH) 24 horas antes do teste.
Serão preparadas suspensões de cultura, diluídas em solução
salina 0,85% utilizando a escala de 0,5 de MacFarland até a
obtenção de aproximadamente 1,5 × 108 Unidades
Formadoras de Colônia (UFC.mL-1) de bactérias.
Pesagem;
Maceração;
Filtragem;
Armazenamento;
Rotoevaporação;
Temperatura;
Testes;
Resultados preliminares;
 Após o período de incubação, observou-se que não houve
inibição visível nos tratamentos utilizados como controle,
assim como nos tratamentos contendo 1mg/mL do extrato de
Pterodon pubescens, a vancomicina na concentração de 30µg
foi capaz de gerar halo inibitório.
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