DHPLC DENATURING HIGH-

PERFORMANCE LIQUID
CHROMOTOGRAPHY
Rute Morais n31483 LBCM

Gentica Molecular A

Fonte:http://coachwasche.com/second.html
DHPLC DENATURING HIGH-
PERFORMANCE LIQUID
CHROMOTOGRAPHY
Tcnica de separao
Sistema de fase reversa

Fonte: http://www.transgenomic.com/pd/Systems.asp?tab=2
 HPLC
completamente
70C-80C desnaturante
Estimar frequncias allicas e mapear
genes envolvidos em doenas complexas
HPLC
parcialmente
50C-70C desnaturante
Deteco de mutaes e SNPs
HPLC no
desnaturante
50C
Purificao de cidos nucleicos
CHROMOTOGRAPHY
PERFORMANCE LIQUID
DHPLC DENATURING HIGH-
DHPLC DENATURING HIGH-
PERFORMANCE LIQUID
CHROMOTOGRAPHY
HPLC parcialmente desnaturante

Fonte: http://www.transgenomic.com/pd/Systems.asp?tab=2
DHPLC DENATURING HIGH-
PERFORMANCE LIQUID
CHROMOTOGRAPHY
Outras aplicaes :
Investigao
microbiana

Quantificao
Aplicaes
da expresso
forenses
gnica

Purificao e Anlise do
Isolamento de estado de
cidos metilao do
nucleicos DNA
DHPLC DENATURING HIGH-
PERFORMANCE LIQUID
CHROMOTOGRAPHY
Referncias bibliogrficas
Keller, G. , Hartmann, A., Mueller, J., Hfler, H. 2001. Denaturing
high pressure liquid chromatography (DHPLC) for the analysis of
somatic p53 mutations. Laboratory Investigation , U.S.A. 81: 1735
Leung, K. H., Yip, S. P. 2008. Denaturing high-performance liquid
chromatography (DHPLC) for nucleic acid analysis In Molecular
Biomethods Handbook (Walker, J. M., Rapley, R. editors). Humana
Press, UK. pp 89-106
Premstaller, A., Xiao, W., Oberacher, H., OKeefe, M., Stern, D.,
Willis, T., Huber, C.G., Oefner, P.J. 2001.Temperature-modulated
array high-performance liquid chromatography. Cold Spring Harbor
Laboratory Press. 11: 1944-1951
Xiao,W.,Oefner,P. J. 2001.Denaturing High-performance liquid
chromatography: A review.HumMutat.17:439-474