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Detecção de Anticorpos
Precipitantes contra Antígenos
Solúveis
Ag
Metodologia
APLICAÇÃO DA TÉCNICA DE IDGA
PARA O SORODIAGNÓSTICO DA
ANEMIA INFECCIOSA EQUINA
Fig. 1. (A) Model of an EIAV particle showing probable locations of the major structural antigens. (B) EIAV proviral genome
organization. The structural proteins of EIAV are produced from polyprotein precursor molecules that are cleaved either by
a virally encoded Protease (Gag/Pol) or by cellular endoproteinases (SU/TM). LTR, long terminal repeat; PR, protease; RT-
RH, reverse transcriptase-RNase H; DU, dUTPase; IN, integrase; tat, transcriptional trans-activator; rev, regulator of viral
mRNA splicing and transport; SU, surface unit glycoprotein; TM, transmembrane glycoprotein.
Pathogenesis (chronic infection)
anemia
virus multiplies in immune
leukocytes complexes
thrombocytopenia
antigenic change
infectious phases
Fever and
viremia
Asymptomatic carriers
infectious
phase
stress
or
viremia steroids
Fig. - Kinetics of adaptive immune responses following experimental infection with EIAV. Humoral
immune responses in terms of relative neutralizing antibody (neut Ab), Env-specific and p26
specific IgG levels are shown along with immunoglobulin avidity index and conformational
dependence measurements (Hammond et al., 1997). Relative levels of Env-specific cytotoxic T-
lymphocyte (CTL) activity during the 36-month observation period are also included.
Atualmente, o único meio seguro de se diagnosticar a infecção pelo vírus
da anemia infecciosa equina (VAIE) é através de um exame de sangue.
Uma vez que o vírus da anemia infecciosa equina sofre alta variação
antigênica, resultando em mutantes com diferentes antígenos, o antígeno
escolhido na prova de Imunodifusão é a proteína principal do core viral: a
p26. Esta proteína mostrou ser altamente conservada em diferentes
variantes isoladas.
(B) (C) (D)
https://www.youtube.com/watch?v=uNVpcHu8jJo
https://www.youtube.com/watch?v=h-KptLVJpU0
https://www.youtube.com/watch?v=6MMKt95SX1w
https://www.youtube.com/watch?v=YH6Bph95lss
https://www.youtube.com/watch?v=yd5nL9iL-M0